Accidents, Traffic ; statistics & numerical data ; Adolescent ; Adult ; Female ; Humans ; Injury Severity Score ; Male ; Maxillofacial Injuries ; epidemiology ; physiopathology ; prevention & control ; Young Adult

OBJECTIVE: To study the bacteriostatic action of Yiqing capsule against propionibacterium acnes and staphylococcus epidermidis in vitro.METHODS: Propionibacterium acnes and staphylococcus epidermidis were cultured by microplating method and routine plating,and the bacteriostatic action of different concentration of Yiqing capsule and its main components against propionibacterium acnes and staphylococcus epidermidis were determined by measuring their MICs.RESULTS: The MICs of Yiqing capsule and Scutellaria baicalensis extractum against propionibacterium acnes and staphylococcus epidermidis were 0.98 mg?mL-1 and 1 mg?mL-1,respectively,whereas the MICs of Radix et Rhizoma Rhei and Rhizoma Coptidis Scutellaria extractum against propionibacterium acnes and staphylococcus epidermidis were 1.96 mg?mL-1 and 1 mg?mL-1,respectively.CONCLUSION: Yiqing capsule showed satisfactory bacteriostatic action against the major pathogenic bacteria of acne.

Objective:To investigate Bcl-2 and Bax gene expression in acute myelogenous Leukemia and the relationship between Bcl-2/Bax ratio and drug resistance.Method:Expression of Bcl-2、Bax was detected by immunohistochemical method while drug resistance of AML was detected by cell culture and MTT assay.Results:A high expression of Bcl-2 and a low expression of Bax were detected in AML,respectively,and the ratio between Bcl-2 and Bax was significantly higher than that in normal control (P＜0.01). The ratio in those who are resistant to chemotherapy was significantly higher than that in those sensitive to chemotherapy (P＜0.05).The responsiveness to chemotherapy of those with a high ratio was poorer than thos with a low ratio (P＜0.05).Conclusion:The alteration of Bcl-2 and Bax played certain role in the development of drug resistance in AML,the test of Bcl-2/Bax ratio may have great significance in choice of chemotherapeutic agents and also provide important information for outcome prediction.

Objective: To investigate the effect of ceramic bovine bone (CBB) combined with hydrogel complex (CBB/HG), ?-tricalcium phosphate (?-TCP) combined with hydrogel complex(?-TCP/HG) on the osteogenesis of bone marrow stromal cells (MSCs) in vitro. Methods: MSCs(induced by 50 mg/L ascorbic acid,10 -8 mol/L dexamethasone and 0.01 mol/L ?-sodium glycerylphosphate) were cultured on CBB/HG , ?-TCP/HG,CBB and ?-TCP respectively. 5 and 10 days after set up, marrow stromal cell attachment and morphology were observed by scanning electronic microscope, the proliferation of the cells was evaluated by cell counting, Alkaline phosphatase(ALP) activity was tested,typeⅠcollagen synthesis were examined by immunohistochemistry stainning. Results: On day 5 and 10 the marrow stromal cells on CBB/HG and on ?-TCP/HG were significantly more than those on CBB or ?-TCP. TypeⅠcollagen synthesis and ALP activity of the cells on CBB/HG and ?-TCP /HG were not different but significantly higher than those on CBB or ?-TCP. Conclusion: CBB/HG and ?-TCP /HG may stimulate bone marrow stromal cell proliferation, differentiation and extracellular matrix synthesis.

ObjectiveTo explore the effect of psychological care and community revisit on preven-tion of postpartum depression. Methods300 cases of pregnant women were randomly divided into the intervention group and the control group with 150 cases in each group. The intervention group took psycho-logical care and community revisit, the control group received conventional care. The incidence of missing follow- up and depression in the two groups were compared using χ2 test. ResultsThe incidence of miss-ing follow-up and depression in the intervention group was significantly lower than those of the control group. ConclusionsPsychological care and community revisit can significantly reduce the occurrence of postpartum depression.

Objective To understand the protection knowledge and practice to cope with common respiratory infection in senior middle school students in Chengdu ,and to provide basis for carrying out school health education and the establishment intervention model of respiratory infection .Methods A self-administered questionnaire was conducted among 2 237 senior one students in two senior middle schools in Chengdu .Results In terms of basic knowledge of common respiratory diseases ,47 .47% of the students had adequate total awareness scores .Respiratory diseases cognitive knowledge was not comprehensive enough .Especially for the under-standing of certain knowledge ,there was misunderstanding .The awareness rates for prevention of respiratory diseases and the treat-ment of behavior were high ,and they were 87 .54% and 71 .43% ,respectively .Conclusion The senior middle school students in Chengdu have some education demand of respiratory disease ,and schools should strengthen publicity and health education .

Objective To summarize the strategies for nursing of skin flaps after 72 hours' transplantation of femoral skin flap for repairing soft tissue defect in lower extremity.Method One hundred and sixty trauma patients with lower extremity soft tissue defect underwent transplantation of femoral skin flap for repairing.The skin flaps were nursed within 72h after transplantation.Results The skins flaps in 160 patients receiving to repair tissue defect of lower limbs all survived.Within 72 hours after operation, 12 patients suffered from vascular crisis, which were cured after pertinent treatment.Conclusions For the patients receiving to repair tissue defect, the skin flaps should be carefully nursed. Meanwhile, preoperative psychological nursing, postoperative pain nursing and health education are critical for the survival of skin flaps.

Purpose To explore the value of ultrasound contrast time-intensity curves in hepatic tumor qualitative diagnosis and evaluation of tumor ablation effect. Materials and Methods Forty-two patients who were clinically diagnosed as hepatic cancer or cirrhosis underwent contrast enhanced ultrasound for the tumor lesions within the liver, dynamic analysis of the lesions were executed with software SonoLiver CAP, the nature of the tumors were determined and the results were compared with those of the enhanced MRI. Ultrasound guided biopsy was followed with radiofrequency ablation treatment, two-dimensional and contrast enhanced ultrasound were executed two weeks after the ablation, and the results were compared with enhanced MRI findings during corresponding period. Results 52 tumor nodules were found with two-dimensional and color Doppler ultrasound, 65 (33 hyperplastic nodules, 32 malignant lesions) with CEUS, 65 (21 hyperplastic nodules, 44 malignant lesions) with ultrasound contrast time-intensity curves, and 61 (20 hyperplastic nodules, 41malignant lesions) with contrast enhanced MRI; pathological examination revealed that 26 of the lesions were benign and 39 were malignant;there was statistically significant difference (χ2=4.561, P<0.05) between the results of ultrasound contrast time-intensity curves and simple contrast enhanced ultrasound for the assessment of the nature of tumor;no statistically significant difference (χ2=0.003, P>0.05) was found between ultrasound contrast time-intensity curves and enhanced MRI for the assessment of the nature of tumor;there was statistically significant difference (χ2=5.298, P<0.05) between ultrasound contrast time-intensity curves and simple contrast enhanced ultrasound for the assessment of whether the tumor was malignant or benign, when taking biopsy results as the reference standard. Conclusion With ultrasound contrast time-intensity curves, dynamical, intuitive, and quantitative evaluation can be obtained for the perfusion differences between the lesion and the surrounding liver parenchyma, which provides more accurate information for qualitative diagnosis of hepatic tumors thus contains great application value in the evaluation of the effects of radiofrequency ablation for hepatic tumors.

China ; Occupational Exposure ; Threshold Limit Values ; Ultraviolet Rays

Objective To summarize the experience of ultrasound-guided percutaneous nephrostomy during percutaneous nephrolithotomy(PCNL) under B-ultrasound guidance. Methods A ultrasound-guided percutaneous nephrostomy was performed in 43 cases by using a 18-gauge needle.Then the percutaneous puncture was expanded for establishing a working tunnel. Under nephroscope,stone fragmentation was completed in the renal pelvis and the upper segment of the ureter.Results The success rate of percutaneous puncture was 100%.Of the 43 cases,the percutaneous working tunnel was successfully established in 42 cases(97.7%),while a conversion to open surgery was needed in 1 case because of uncontrolled hemorrhage after puncture.The overall stone clearance rate was 100%(42/42),including 38 cases of stone removal on one session and 4 cases of two-staged clearance.No serious complications,such as massive hemorrhage or neighboring organ injuries,were encountered.Re-examinations with B-ultrasonography or KUB at 1 month after operation showed no residual stones.Conclusions Ultrasound-guided percutaneous nephrostomy is minimally invasive,safe,and reliable.Proper use of this technique is crucial for a successful performance of percutaneous nephrolithotomy in the treatment of upper urinary tract calculus.