OBJECTIVE:To determine the serum concentration of Deoxyschisandrin by RP-HPLC.METHODS:The chr-omatographic separation was performed on Phenomenex C18(250mm?4.6mm,5?m) column with column temperature at 30℃.The mobile phase consisted of methanol - water (80∶20) at a flow rate of 1mL?min-1. The detection wavelength was set at 252nm. RESULTS:The calibration curve of Deoxyschisandrin was linear in the concentration range from 0.52 to 33.28?g?mL-1 (r=0.999 6). The average recovery of deoxyschisandrin was (97.27?4.11)%(RSD=4.23%,n=9).CONCLUSION:The method is simple, accurate and sensitive, and it can be used for the determination of serum concentration of Deoxyschisandrin.

To compare posterior choroidal thickness in high myopia amblyopia eyes at different points to high myopia and normal eyes of Chinese children and investigate the relationships between choroidal thickness, axial length and age.METHODS: Fifty Chinese children (65 eyes) with age 4~15 years ( mean 9. 91 ± 3. 41 years) were recruited. By atropine optometry they were divided into high myopia amblyopia group ( 24 eyes ) , high myopia group ( 19 eyes ) , and normal group ( 22 eyes ) . Choroidal scans were obtained for all eyes using enhanced depth imaging spectral-domain optical coherence tomography ( EDl-OCT) . Subfoveal choroidal thickness (SFCT), macular thinkness, choroidal thickness and retinal thickness at 0. 5, 1. 0, 1. 5, 2.0, 2.5, 3.0mm superior (S, 12:00 position), temporal ( T, 9:00 position) , inferior ( l, 6:00 position) , nasal ( N, 3:00 position) were measured. Meanwhile, axial lengths of all eyes were measured by A-Scan. RESULTS: Compared high myopia group and emmetropia group, SFCT and the thickness of choroids on each position were thinnest in high myopic amblyopia group, with statistically significant differences (P<0. 05). There was a significant negative correlation between SFCT and axial length in high myopic amblyopia group (r=-0. 531, R2 =0. 282, F=7. 476, P=0. 013), with no relative in age (r=-0. 292, R2=0. 085, F=2. 044, P=0. 167).CONCLUSlON: The choroidal thickness thinning in high myopic amblyopia shows a negative correlation with axial length.

·AlM:To investigate the retinal thickness change of high myopia amblyopic children, so as to discuss the relationships between the retinal thickness of central fovea of macula and the factors of axis oculi and age. · METHODS:Thirty-nine children ( 65 eyes ) with the average age of ( 9.91 3.41 ) years were recruited.All eyes were ruled out the pathological changes of fundus diseases and front section. After a tropine optometry, they were divided into three groups: high myopia amblyopic group ( 24 eyes ) , high myopia group ( 19 eyes) and normal group ( 22 eyes ) .Retinal scans were obtained for all eyes using Heidelberg optical coherence tomography ( OCT ) . Subfoveal macular thickness, retinal thickness at 0.5mm, 1.0mm, 1.5mm, 2.0mm, 2.5mm, 3.0mm superior ( S, 12∶00 position), temporal (T, 9∶00 position), inferior (l, 6∶00 position) and nasal (N, 3∶00 position) from the fovea were measured and axial length was also surveyed by A -ultrasound. Statistical analyses were performed to evaluate retinal thickness at each location and to correlate subfoveal macular thickness with axial length and age.
·RESULTS:The average subfoveal macular thinkness of the high myopia amblyopic group was thinner than high myopia group but thicker than normal group.There was no statistical difference between three groups (P>0.05). Retinal thickness inferior to the fovea at 0.5mm temporal and superior to the fovea in the high myopia amblyopic group at 1.0mm temporal were both thinner than normal group which had statistically significant ( P <0.05 ). Retinal thickness on nasal, superior, temporal, and inferior at 1.5mm, 2.0mm, 2.5mm, 3.0mm from the fovea were measured, high myopia amblyopic group were the thinnest in the three groups, and there was statistically significant between three groups ( P<0.05). There was no correlation between the average subfoveal macular thickness and axial length, age in high myopia amblyopic group.
· CONCLUSlON:There are significant abnormalities of macula retinal structure in high myopia amblyopic children.

AIM: To research the peripapillary retinal nerve fiber layer ( RNFL ) thickness change in high myopia amblyopic children and to discuss the relationships among RNFL thickness, axial length and age.
METHODS:Thirty-five Chinese children (59 eyes) with a mean age of ( 9. 59 ±2. 90 ) years were recruited. All eyes were ruled out the pathological changes of fundus diseases and front section. By atropine optometry after they were divided into: high myopia amblyopia group (22 eyes), high myopia group (15 eyes), normal group (22 eyes) . RNFL scans were obtained for all eyes using optical coherence tomography and axial length was also surveyed by A - ultrasound. Statistical analyses were performed to evaluate RNFL thickness at each location with axial length and age.
RESULTS:The peripapillary RNFL thickness in temporal of high myopia amblyopia group was thinner than that in
high myopia group, and thicker than that in normal group. The peripapillary RNFL thickness in nasal, superior, inferior and the average thickness of high myopia amblyopia group were thinner than those in high myopia and normal gruops. The peripapillary RNFL thickness in inferior and average thickness of high myopia amblyopia group were significantly thinner than those of high myopia (P<0. 05). The peripapillary RNFL thickness in nasal, superior, inferior and the average thickness of high myopia amblyopia group were significantly thinner than those of normal (P<0. 01). The peripapillary RNFL thickness in temporal of high myopia group was significantly thicker, and in nasal, superior, inferior and the average thickness were significantly thinner than those of normal (P<0. 05). The thickness of peripapillary RNFL in inferior showed a negative correlation with axial length in high myopia amblyopia group (R=0. 474, R2=0. 225, F=4. 933, P=0. 040). The thickness of peripapillary RNFL in superior showed a negative correlation with axial length in high myopia group (R=0. 642, R2=0. 412, F=9. 104,P=0. 010). These were no correlation between the peripapillary RNFL thickness and age in high myopia amblyopia, myopia amblyopia and normal.
CONCLUSION:There are significant abnormalities of retinal structure in high myopia amblyopia.

Objective:To study the pupils' anxiety and related factors.Methods:6994 pupils and their were investigated using Conners Behavior Checklist(PSQ).Results:The pupils above 8 years old were more anxious.The economic state of family played an important role in pupils' anxious.Their existed significant age difference in boys but not in girls.Level of farther's and mother's education has obvious influences in their anxiety.Conclusion:The effect facts of anxiety include the age of students,economic state of family,the level of parent's education.

97 cases of jaw bone fractures were treated with rigid internal fixation.16 cases had malocclusion postoperation.The etiology was analyzed and the prevention methods were proposed.Intermaxillary elastic traction or fixation and craniomaxillofacial fixation or craniomentum elastic traction were performed to treat the malocclusion.By this way,14 cases were cured.1 case was operated again to resume the normal occlusion,1 case received occlusal adjustment.Incomplete reduction,incorrect operative performance,deficiency of postoperative intermaxillary fixation and incorrect intermaxillary fixation were the main causes to malocclusion.So anatomic in time reduction,correct operative performance,intermaxillary fixation,application of craniomaxillofacial fixation or craniomentum elastic traction instead of intermaxillary fixation for some special cases are effective methods to correct postoperative malocclusion.

Objective To observe the expression of osteoprotegerin (OPG), receptor activator of nuclear factor κβ ligand(RANKL) and receptor activator of nuclear factor κβ (RANK) in bone tissue of rats with chronic fluorosis and to explore the relation between OPG/RANKL/RANK system and bone damage in chronic fluoride poisoning rat and the antagonism effects of Danlan Xianpeng capsule. Methods SD rats were randomly divided into six groups according to body weight (equal male and female in each group): fluorosis group, high dose drug group, medium dose drug group, low dose drug group, control group, borax group(positive control), 12 rats in each group. The control group drank tap water and the remaining 5 experimental groups consumed 50 mg/L fluoride water, and high, medium and low doses drug group took Danlan Xianpeng capsule at doses of 0.8,0.4,0.2 g/kg,borax group took borax at dose of 0.8 g/kg. OPG, RANKL, RANK protein in rat tibial metaphysis was detected by immunohistochemistry at the 6 month. Results Compared with the control group(173.79 ± 5.23, 174.17 ± 5.01,155.63 ± 7.11), the expressions of OPG, RANKL were increased and the expression of RANK was decreased in fluorosis group(156.83 ± 5.80, 157.74 ± 6.70, 173.92 ± 4.37), the difference was statistically significant (all P ＜ 0.05). Compared with the fluorosis group, the expression of OPG and RANKL were decreased and the expression of RANK was increased in high-dose drug group, middle-dose drug group(169.67±5.07, 168.08 ± 5.05,162.12 ± 4.24, 170.78 ± 5.01, 168.41 ± 7.19, 166.69 ± 5.78, all P ＜ 0.05). Compared with the borax group (167.27 ± 4.08, 167.85 ± 5.01, 166.14 ± 3.95), the expression of OPG and RANKL was increased in the low-dose drug group (163.40 ± 4.11, 159.49 ± 5.78), the expression of RANK was increased in the high-dose drug group (162.12 ± 4.24) and decreased in the low-dose drug group(171.54 ± 8.06), the difference was statistically significant (all P ＜ 0.05). Conclusions Chronic fluoride poisoning can cause increased bone turnover and enhance the activity of osteoelastic absorption by increasing RANKL. Danlan Xianpeng capsule can affect bone remodeling through the OPG/RANKL/RANK system, and antagonises bone damage caused by fluoride.

Hematologic Diseases ; Hemolysis ; Humans ; Infant, Newborn

Objective To assess the relationships between bile reflux gastritis (BRG) and Helicobacter pylori infec-tion in children. Methods A total of 258 BRG cases were included in BRG group and 1 749 gastric mucosal inflammation cases without bile reflux were used as control group. Children with BRG were divided into three groups according to the dis-ease classification and they were also sub-divided into three groups according to the bile reflux indexing. The infection rates of Helicobacter pylori were analyzed in different groups. Results Compared to control group (34.02%), the infection rate of Helicobacter pylori was significantly higher in BRG group (46.12%). The infection rates of Helicobacter pylori infection were 40.86%(38/93), 45.53%(56/123) and 59.52%(25/42) in patients with mild, moderate and severe infections. There was no significant difference between them (χ2=4.089, P>0.05). The positive rates of Helicobacter pylori infection were 37.32%(53/142), 53.68%(51/95) and 71.43%(15/21) in children withⅠ,ⅡandⅢreflux indexing, and there was significant differ-ence between them (χ2=12.022,P<0.01). Conclusion Helicobacter pylori infection is one of the causative factors leading to BRG in children. The bile regurgitation increased with the increasing of Helicobacter pylori infection.

Objective To select the calibrator for the conventional measurement system of serum a-Amylase (Amy).Methods The Amy levels of forty frozen serum samples were detected by the IFCC reference method (reference system),the conventional system A which used the Bioassay routine reagent and Randox calibrator,and was calibrated by the Roche PNPG7 method,and the conventional system B which used the Bioassay routine reagent and Randox calibrator,and was calibrated by the Rondox liquid stable PNPG7 method,respectively,and the acceptability of the two conventional systems was evaluated.Results The regression equations of the measurement values between the IFCC reference method and the conventional systems A and B were Y =0.964X +0.376 and Y =1.095X + 3.131,respectively.Among them,X and Y represented the results of the IFCC reference method and the conventional system,respectively.Compared with the IFCC reference method,the results of the conventional system A was reliable.Condusion With the guidance of the IFCC reference method,the domestic biochemical reagents matched with the suitable calibrators may provide the acceptable results.