Angiogenesis after cerebral ischemia iS increasingly receiving attention.The expression of angiogenesis growth factor increases after cerebral ischemia.It promotes endothelial proliferation and neovascularization,and thus improves blood supply in ischemic regions and decreases the volume of infarction.The combination of exogenous angiogenesis growth factor.stem cell transplantation and therapeutic angiogenesis to promote angiogenesis was mainly achieved by promoting the expression of angiogenesis growth factor."Ihe further study of the mechanism of angiogenesis after ischernia will provide theoretical basis for the treatment of ischemic stroke.

Objective To evaluate the influence of nursing intervention on the self-care of patients with high risks of diabetic foot. Methods Among 60 diabetic patients who have risks of indeed getting diabetic foot. Those risks include peripheral vascular disease and neuropathy, we gave them self-care ability education and nursing intervention. Each patient was given personal self-nursing education. Those patients were followed for 6 months, and were instructed with everyday foot nursing, and self-monitoring management, etc. A year later, we investigated the self-management capability of those patients, and came out with the statistical data of those who go hospital again or catch diabetic foot. Results Before the education, only 16.6％ patients can recognize the risks of diabetic foot, 15％ patients nurse the feet by themselves daily, 13.3％ patients grasp the methods to discern and process the normal foot problems in all 60 patients. After nursing intervention, 84.3％ patients can recognize the risks of diabetic foot, 85％ patients nurse the feet by themselves daily, 86.6％ patients grasp the methods to discern and process the normal foot problems. Diabetic patients with high risks got more nursing knowledge and self-nursing ability after nursing intervention. (t =22. 71,-19.90;P ＜0. 05). 0nly one sample caught diabetic foot. Conclusions Nursing invention can promote the serf-care ability d patients. It has an important role in controlling the diabetic foot risk factors and preventing disease.

Emerging data indicated that HCV subverts the antiviral activity of interferon (IF); however,whether HCV core protein contributes to the process remains controversial. In the present study, we examined the effect of HCV core protein on interferon-induced antiviral gene expression and whether the effect is involved in the activation and negative regulation of the Jak/STAT signaling pathway. Our results showed that, following treatment with IFN-α, the transcription of PKR, MxA and 2'-5'OAS were down-regulated in HepG2 cells expressing the core protein. In the presence of HCV core protein,ISRE-dependent luciferase activity also decreased. Further study indicated that the core protein could inhibit the tyrosine phosphorylation of STAT1, whereas the level of STAT1 expression was unchanged.Accordingly, SOCS3, the negative regulator of the Jak/STAT pathway, was induced by HCV core protein. These results suggests that HCV core protein may interfere with the expression of some interferon-induced antiviral genes by inhibiting STAT1 phosphorylation and induction of SOCS3.

Objective To summarize the clinical characteristics and make genetic diagnosis in the patients with hereditary spinocerebellar ataxia type 7 (SCA7).Methods Pedigree analysis and clinical examination were performed in one family with SCA7 by clinical findings,of which retinal morphology and visual electrophysiology were available on part numbers.The polymorphic cytosine adenine guanine (CAG) repeats in the encode region of SCA7 gene were detected by combining polymerase chain reaction with deoxyribonucleic acide (DNA) sequencing on 19 familial numbers and 12 controls.Results 6 patients were identified,who manifesting cerebellar ataxia,decreased visual acuity and colour vision defect,as was pigmentary retinopathy on fundoscopy;The 6 patients had not only extinction of the electroretinogram (ERG) but also remarkably reduced amplitudes of oscillatory potentials and flash-visual evoked potentials. On normal alleles CAG repeat size ranges from 8 to 25 repeats,wherease on mutated alleles of the 6 numbers it ranges from 50 to 97 repeats.The 6 numbers were diagnosised as SCA7 patients.One asymptomatic individual of this family,who displayed a normal allele with 18 CAG repeats and another containing abnormal expantion of 56 repeats,was diagnosised as a asymptomatic carrier whose age maybe still below the age of onset.Conclusion The clinical manifestations of SCA7 are heterogeneous,and the detection of CAG repeats can provide an effective way for the gene diagnosis and the prediction of asymptomatic patients.

Objective To analyze the epidemiological characteristics of Japanese encephalitis (JE) in Zhejiang Province from 2007 to 2016 for developing the measures of control and prevention. Methods The data were collected from national notifiable diseases registry system (NNDRS) and case-based JE surveillance system (JESS) from 2007 to 2016, and they were analyzed through descriptive epidemiological method and Microsoft Office Excel 2010. Results From 2007 to 2016, a total of 595 JE cases were reported in Zhejiang Province, with an average annual incidence of 0.114 per 100000 population, among which, 559 (93.95%) were laboratory confirmed. During the ten years, 20 cases died and the average annual case fatality rate was 3.36% . Cases were distributed mainly in Wenzhou, Ningbo and Taizhou Cities, which accounting for 54.79% of the total.The peak months were July, accounting for 86.89% of all the cases.And 85.71% of all the cases were in the age of 0-14 years and 47.73% were scattered children.And 22.77% of the JE cases were vaccinated but 14.01% of them did not complete the whole course.And 77.23% of the cases did not have the experience of vaccination or unknown.Conclusion The incidence of JE is decreasing. The occurrence of JE is sporadic with distinct seasonal peak and mainly concentrating in young-age children.

OBJECTIVETo analyze and compare the constituents of the essential oils from the fresh and dried pericarps of Citrus sinensis.

METHODThe essential oils from the fresh and dried pericarps of C. sinensis were extracted by water steam distillation method and analyzed by GC-MS.

RESULT28 and 25 components were identified from the essential oils of the fresh and dried pericarps of C. sinensis respectively, 37 components were identified from both samples totally, among which 26 components were identified in the essential oils of the pericarps of C. sinensis for the first time.

CONCLUSIONThe most components in both oil samples were similar and main component of the both oils was limonene (90. 16% and 77. 34%).

Citrus sinensis ; chemistry ; Cyclohexenes ; analysis ; chemistry ; isolation & purification ; Fruit ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods ; Oils, Volatile ; analysis ; chemistry ; isolation & purification ; Plant Oils ; analysis ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Terpenes ; analysis ; chemistry ; isolation & purification

This study was aimed to investigate the immunomodulatory ability of human umbilical cord mesenchymal stem cells (UB-MSC) along with prolonging of culture time and increasing of passages in vitro. Mesenchymal stem cells (MSC) were isolated from human umbilical cord and cultured in vitro. The morphological changes and nucleocytoplasmic ratio of MSC were observed using Giemsa staining. MSC of the 5th passage were selected as control group, and MSC of the 13th passage were taken as senile group. The degree of cell senescence was detected by aging cells in situ test kit. Cell Counting Kit-WST-8 was used to determine the proliferation of lymphocytes in mixed lymphocytes coculture system with different passages of MSC. The expression of immunomodulation-related genes was detected by RT-PCR. The results showed that the length-breadth ratio of MSC increased and nucleocytoplasmic ratio decreased along with the increasing of passages. The senium degree of cells of the 13th passage was higher than that of the 5th passage cells. The capacity of suppressing lymphocyte proliferation of the 13th passage MSC was enhanced, compared with the 5th passage. Moreover, the expression of immunosuppression-related genes of senile MSC increased and the expression of most anti-inflammation associated genes declined as compared with young MSC by RT-PCR. It is concluded that the degree of MSC senescence gradually develops with increasing of culture passage, but the immunosuppressive ability of MSC strengthens with continuous culture.
Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Cellular Senescence ; Humans ; Lymphocytes ; cytology ; immunology ; Mesenchymal Stromal Cells ; cytology ; immunology ; Umbilical Cord ; cytology

Aorta ; pathology ; surgery ; Aortopulmonary Septal Defect ; therapy ; Balloon Occlusion ; methods ; Catheterization, Swan-Ganz ; methods ; Child ; Female ; Humans ; Pulmonary Artery ; pathology ; surgery ; Treatment Outcome

BACKGROUND: Phenylephrine has been proved to exert a protective effect on radiant-induced salivary gland and epithelial cell injuries, but its effect on hydrogen peroxide (H2O2)-induced oxidative stress in osteoblasts are not fully understood. OBJECTIVE: To explore the effect of phenylephrine on H2O2-induced oxidative stress in osteoblasts, and to explore the mechanism underlying the regulation by the expression level of nicotinamide phosphoribosyltransferase (Nampt). METHODS: Primary osteoblasts were cultured and randomly divided into four groups: blank control group, H2O2group, phenylephrine group, and combination group (0.5 hour pretreatment of 1×10-5mol/L phenylephrine, and then given 300 μmol/L H2O2). The morphology of osteoblasts was observed at different time points. Osteoblasts were collected after 24-hour culture, and total RNA and protein were then extracted to detect the mRNA and protein expression levels of Nampt by RT-PCR and western blot assay, respectively. RESULTS AND CONCLUSION: Compared with the blank control group, reduced osteoblasts and evident cell shrinks were observed in the H2O2group, while the number of osteoblasts significantly increased in the combined group compared with the H2O2group at 12, 24 and 48 hours of culture. RT-PCR results showed that the mRNA level of Nampt in the H2O2group was reduced by 31.23% of that in the blank control group, while the mRNA level of Nampt in the combination group was dramatically increased by 206.20% of that in the H2O2group at 24 hours of culture (both P < 0.05). Furthermore, western blot assay findings revealed that the protein level of Nampt in the H2O2group was reduced by 67.98% of that in the blank control group, while the protein level of Nampt in the combination group was increased by 152.25% of that in the H2O2group at 24 hours of culture (both P < 0.05). Our results indicate that phenylephrine can alleviate the shrink and atrophy of osteoblasts caused by H2O2, thereby exerting protective effect by up-regulating the mRNA and protein levels of Nampt that may be a regulatory gene.

OBJECTIVETo analyze the mutations of candidate POU3F4 gene in the Chinese pedigree with Y linked hereditary hearing impairment.

METHODSPolymerase chain reaction (PCR) reactions were performed with five pairs of primer in the coding sequence of POU3F4 gene. PCR-single-strand conformation polymorphism (PCR-SSCP) was subsequently applied in the 43 individuals of DFNY1 family for screening the gene mutations.

RESULTSThe PCR amplification fragments showed well quality in the five pairs of primer and further analysis with PCR-SSCP showed no any polymorphism and mutations in the members.

CONCLUSIONSThe possibility of the deafness gene POU3F4, which locates on the translocation region on X and Y chromosome, contributed to the Y linked family deafness was successfully ruled out. It may imply that the causal gene of the DFNY1 family locate on the Y chromosome.

Adolescent ; Adult ; Aged ; Asian Continental Ancestry Group ; genetics ; Child ; DNA Primers ; Deafness ; genetics ; Female ; Genetic Diseases, Y-Linked ; genetics ; Humans ; Male ; Middle Aged ; POU Domain Factors ; genetics ; Pedigree ; Point Mutation ; Polymorphism, Single-Stranded Conformational ; Young Adult