2.Effects of trichostatin A on HDAC8 expression, proliferation and cell cycle of molt-4 cells.
Jing, HE ; Hongli, LIU ; Yan, CHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2006;26(5):531-3
The effects of Trichostatin A (TSA) on histone deacetylase 8 (HDAC8) expression, proliferation and cell cycle arrest in T-lymphoblastic leukemia cell line Molt-4 cells in vitro were investigated. The effect of TSA on the growth of Molt-4 cells was studied by MTT assay. Flow cytometry was used to examine the cell cycle. The expression of HDAC8 was detected by using immunocytochemistry and Western blot. The results showed that proliferation of Molt-4 cells was inhibited in TSA-treated group in a time- and dose-dependent manner. The IC50 of TSA exposures for 24 h and 36 h were 254.3236 and 199.257 microg/L respectively. The cell cycle analysis revealed that Molt-4 was mostly in G0/G1 phase, and after treatment with TSA from 50 to 400 microg/L for 24 h, the percents of G0/G1 cells were decreased and cells were arrested in G2/M phase. Treatment of TSA for 24 h could significantly inhibit the expression of HDAC8 protein in Molt-4 cells (P<0.01). It was concluded that TSA could decrease the expression of HDAC8 in Molt-4 cells, which contributed to the inhibition of proliferation and induction of cell cycle arrest in Molt-4 cells.
3.Analysis the complications after trabeculectomy in 405 cases in department of ophthalmology
Xing-Yun, YAN ; Ping, HE ; Jing, LIU
International Eye Science 2015;(4):707-708
AIM: To analyze and summarize the effect of glaucoma trabeculectomy in the department of ophthalmology in basic hospital.
METHODS: Postoperative intraocular pressure ( IOP ) , filtering bleb and complications of 316 cases (405 eyes) of patients with glaucoma after trabeculectomy were analyzed.
RESULTS: After follow- up 12mo, 76. 5% IOP was controlled in normal level. 42. 5% was filtration bleb typeⅠ, 33. 1% typeII, 14. 6% in typeⅢand 9. 9% in typeⅣ. Intraoperative complication rate was 2. 5%, that was 31.4% at postoperative early stage ( before discharge ) , and 6. 7% at postoperative long - term ( 6mo after discharge) .
CONCLUSION:Trabeculectomy for glaucoma can better solve the problem of high IOP. It is more mature in primary hospitals, but there are still a variety of intraoperative and postoperative complications.
4.Supervision on Measurement of Quality and Safety on Medical Treatment Facility
Liangcai ZHANG ; Jing LI ; Yan HE
Chinese Medical Equipment Journal 2003;0(12):-
To combine "the rule of military medical measurement" with the current situation of medical treatment quality control, the foundation, important component and necessary condition of medical treatment quality control was described. Some idiographic advices of intercoordination and communication in medical measurement institution, the direction and training of special technique, and how to develop compulsion coerciveness checking in base course were given. These are valuable for insuring medical treatment facility in hospital.
5.Optimization of Ethanol Extraction Technology for Xiaoluo Granules by Orthogonal Experiment
Yan WANG ; Weimin CHEN ; Yan HE ; Jing DU ; Yinghua FU
China Pharmacist 2014;(9):1486-1488
Objective:To optimize the ethanol extraction technology for Xiaoluo granules. Methods:The extraction technology for the ethanol dissolvable ingredients in 10 kinds of medicinal materials was optimized by orthogonal experiment. The weighted sum of ba-icalin content and the dry extract rate as the index, 4 influencing factors with 3 levels each were optimized by L9 (34 ) orthogonal table. Results:The optimal ethanol extraction conditions were as follows:the medicinal material was soaked by 10-fold 50% ethanol solution for 45min, and then extracted by heating reflux for 3 times with 1. 5h each time. Conclusion: The method can effectively extract the ethanol dissolvable ingredients in 10 kinds of medicinal materials, which can be used as the ethanol extraction technology for Xiaoluo granules.
6.Modulatory effect of curcumin on the adhesion and migration of Raji cells
Qiuling WU ; Yan CHEN ; Chun ZHANG ; Jing HE
Journal of Chinese Physician 2000;0(12):-
Objective To study the effect of SDF1/CXCR4 and FN/?1 integrin modulated by curcumin on the adhesion and migration of B lymphoma Raji cell and to explore the mechanism of curcumin to inhibit the invasiveness of lymphoma.Methods The experiment consisted of eight groups: Control,FN,SDF,FN+anti-?1 integrin,SDF+anti-CXCR4,curcumin+FN,curcumin+SDF.Adhesion assay and migration assay were used to detect the difference of invasiveness among different groups.Results In vitro,SDF1 and FN can increase the adhesion and migration of Raji cells,which can be blocked by ?1 integrin antibody and CXCR4 antibody.Curcumin can inhibit the adhesion and migration of Raji to FN in a dose-dependant manner.Low dosage curcumin had minimal effect on the adhesion and migration of Raji induced by SDF except that 25?mol/L curcumin can inhibit the adhesion and migration of Raji.Conclusion FN/?1 integrin and SDF1/CXCR4 pathway play important roles in the adhesion and migration of Raji cells.Curcumin can decrease the invasiveness of Raji cells through inhibiting FN/?1 integrin.It has good prospect of clinical application.
7.Effects of Meliorated Renal Failure Decoction on Renal Function and Inflammatory Cytokines in Patients with Chronic Kidney Disease at 3-4 Stage
Jing YANG ; Ruijun YAN ; Chen WANG ; Liqun HE
Chinese Journal of Information on Traditional Chinese Medicine 2014;(12):15-18
Objective To investigate the effects of meliorated renal failure decoction (MRFD) on renal function and inflammatory cytokines in patients with CKD 3-4 stages. Methods Sixty-two patients with CKD 3-4 stages were divided into treatment group and control group through random number table method. Both groups were given conventional treatment of western medicine, and the treatment group was given MRFD additionally. After 6 months of treatment, the changes of TCM symptom scores, Hb, Scr, BUN, 24 h UP, IL-6, IL-8, TNF and eGFR were observed. Meanwhile, the clinical efficacy was evaluated. Results The total clinical effective rate in treatment group was 65.63%(21/32), and 30.00%(9/30) in the control group (P<0.01);the effective rate of TCM syndromes in treatment group was 96.88%(31/32), and 53.33%(16/30) in the control group (P<0.01). The symptoms were improved better in the treatment group than the control group except for the symptoms of dry mouth and throat, and diarrhea. The level of eGFR in treatment group increased obviously (P<0.05), while the levels of Scr, 24 h UP and BUN decreased (P<0.05, P<0.01), with statistical significance between the two groups ( P<0.01). The level of eGFR increased, and the levels of Scr decreased in the control group (P<0.01). The levels of IL-6, IL-8, and TNF in both groups were reduced, while the level of Hb increased in the two groups, without statistical significance (P>0.05). Conclusion MRFD can improve TCM syndrome and renal function, delay the progress of patients with CKD at 3-4 stage by means of reducing the inflammatory cytokines and inhibiting micro-inflammatory state.
8.Liver cancer stem cell isolation and biological characteristics
Yan LIU ; Yuesu ZHOU ; Jing HE ; Peng LIU
Chinese Journal of Tissue Engineering Research 2014;(28):4549-4554
BACKGROUND:Normal stem cells and tumor stem cells have differences in cellsignaling pathway of gene expression and dependence. How to find a therapeutic method of selectively kil ing tumor stem cells is a topic that should be studied greatly. OBJECTIVE:To isolate human liver cancer stem cells MHCC97 and to investigate the biological characteristics of them. METHODS:Tumor stem cells were selected from highly metastatic human hepatoma cellline MHCC97 by flow cytometry. CD133-CD34-MHCC97 from normal human liver stem cells and CD133+CD34+MHCC97 from tumor stem cells were isolated. Their phenotypes, growth curve, cellcycle and multi-lineage differentiation were measured. RESULTS AND CONCLUSION:Phenotypes of CD133+CD34+MHCC97 cancer stem cells were CD133+and CD34+and they had the same growth curve and cellcycle with the liver stem cells CD133-CD34-MHCC97, besides, they had the differential ability to epithelial cells and endothelial cells that proved to be stem cells. These results indicated that human cancer stem cells CD133+CD34+MHCC97 had the specific characteristics of tumor stem cells, could differentiate into epithelial cells and endothelial cells, had the biological property of tumor stem cells, was an origin of tumor relapse and metastasis, and a target of clinical therapy.
9.On the treatment of chronic obstructive pulmonary disease from qi-blood-body fluid
Shuqiang LI ; Jing YAN ; Yange CAI ; Qiyang HE
International Journal of Traditional Chinese Medicine 2013;(6):523-525
The pathogenesis of chronic obstructive pulmonary disease is the dysfunction of qi-blood-body fluid.By the analysis of the pathogenesis of different stages of COPD,qi deficiency,phlegm,blood stasis is the key to COPD.According to the pathogenesis of syndrome differentiation,a good curative effect can be achieved by the selection of different treatment methods like tonifying qi,eliminating phlegm and removing blood stasis.
10.Effect of Electroacupuncture on the INS Level and CCK Content in a Rat Model of DGP
Fenge HE ; Quanquan WAN ; Yaping LIN ; Yan PENG ; Jing SHEN
Shanghai Journal of Acupuncture and Moxibustion 2016;(1):81-84
Objective To investigate the effect of electroacupuncture on serum insulin (INS) levels and gastric antral cholecystokinin (CCK) content in rats with diabetic gastroparesis (DGP).Methods Sixty SD rats were randomized into groups A, B, C, D and E, 12 rats each. Group A is a normal control. A DGP model was made by an intraperitoneal injection of 2% streptozocin (STZ) plus an irregular high sugar and fat diet in groups B, C, D and E. group B is a model one and did not receive treatment. Group C received electroacupuncture at points Zusanli, Sanyinjiao and Liangmen; group D, electroacupuncture at the control points of Zusanli, Sanyinjiao and Liangmen. Group C, an oral gavage of metoclopramide tablet solution. Blood sugar and urine sugar were determined using a OneTouch lood glucose meter and Tes-Tape, respectively. The gastric emptying rate and the intestinal migration rate were measured by an oral gavage of phenol red. Serum INS levels and gastric antral CCK content were measured by ELISA in every group of rats before and after treatment.Results There were statistically significant differences in blood and urine sugar values between group B, C, D or E rats and group A (P<0.01) and between group C rats and group B or E (P<0.05). There was a statistically significant difference in the gastric emptying rate between group B, C, D or E rats and group A (P<0.01) and between group C rats and group B or D (P<0.01,P<0.05). There was a statistically significant difference in the intestinal migration rate between group B or D rats and group A (P<0.01) and between group C rats and group B or D (P<0.01). There were statistically significant differences in serum INS levels and gastric antral CCK content between groups B and A rats (P<0.05,P<0.01). There was a statistically significant difference in gastric antral CCK content between groups D and A rats (P<0.01). There were statistically significant differences in serum INS levels and gastric antral CCK content between groups C or E rats and group B (P<0.05,P<0.01). There was a statistically significant difference in gastric antral CCK content between groups C and D rats (P<0.05).Conclusions Electroacupuncture can markedly improve gastrointestinal functions and promote gastric emptying in DGP rats. Its therapeutic effect may be related to electroacupuncture raising serum INS levels and reducing gastric antral CCK content.