To investigate the effects of Xuefu Zhuyu Decoction, a compound Chinese herbal medicine, on the proliferation and extracellular matrix synthesis of cardiac fibroblasts (CFs) from neonatal rats induced by angiotensin II, and to explore the mechanism of Xuefu Zhuyu Decoction in improving myocardial fibrosis.

Targeting characteristic information processing of TCM pulse diagnosis, this article studied the application of fuzzy mathematics in objectifying pulse diagnosis. By analyzing limitations of traditional analytic methods, this article offered the scientific evidence of using mathematical algorithm in pulse diagnosis. At the same time, combined with actual clinical data, it also verified the correctness of introduction mathematical algorithm in TCM pulse diagnosis.

Objective:To investigate the feasibility of adipose tissue-derived stem cells(ADSCs) as the seeding cells of skin engineering for skin defect repair. Methods :Adipose tissues were isolated from a SD rat groin, the fat was cut and incubated in a collagenase solution, and then the ADSCs were cultivated in culture medium. The amplified cells in vitro were inoculated in collagen gel to form tissue engineered skin. Fifteen SD rats were randomly divided into three groups: experimental group was treated with ADSCs collagen tissue engineered active skin substitute, and single collagen and blank were served as two control groups. The materials were grafted onto total skin defect wounds on the back of rats in the corresponding group respectively. The effect of wound repair was observed histologically. Results:The time of wound healing in the ADSCs and collagen experimental group, the single collagen control group and the blank control group were (14.3?1.7) days,(16.9?2.5)days,and (21.2?4.2)days respectively, and showed significant differences(P

Objective: To investigate the cause and mechanism of oral lichen planus (OLP). Methods: The expression of cyclins dependent kinases (CDK4) in epithelial cells in the samples in 40 cases of OLP and in 10 cases of healthy controls was observed by immunohistochemical method. Results: The expression indexes of CDK4 in the epithelial basal layer and spinous layer in OLP were 0. 5637 ? 0. 0201 and 0. 5388? 0. 0120, those in the healthy control were 0. 4020? 0. 0155 and 0. 3480? 0. 0163, respectively(p

An actinomycete B37 was isolated from an intertidal marine sponge Hymeniacidon perleve, which has strong activity against Gram positive bacteria and moderate activity against tumor cells. The mycelium and spore morphology, physiological properties and 16SrDNA sequence suggested that B37 is Streptomyces pseudogriseolus. The fermentation conditions of this strain were investigated for the biosynthesis of bioactive metabolites.

To assess the impact of the operational simulation training system on the cultivation of ophthalmic students.
●METHODS: Four hundred and eighty ophthalmic students in China Medical University from 2009 to 2011 were chosen as objects and divided into two groups at random. The students in the experimental group used operation simulation training system combined with traditional teaching methods, while the students in the control group only used traditional teaching methods. We examined the operation skills and the confidence of all the students half a year later.
●RESULTS: The improvement of the operation skills during the suture technique examination, and the confidence for operation technology were significantly different in two groups. The basic skills of the students in the examination groups improved apparently during the operational simulation training and they were full of confidence to their operational technique.
●CONCLUSlON: The operational simulation training is useful for promoting the clinical ability for ophthalmic students and should be widely popularized.

Background Streptozotocin (STZ)-induced type 1 diabetic model is an acceptable model and is often used to the study of diabetic retinopathy (DR).However,the model is often established using retinal digest preparation in vitro in albino rats,and therefore it is difficult to evaluate the change of retinal vessels by fundus fluorescein angiography (FFA) in vivo.Recently,pigmented rats are being used as the DR model.But the study on the comparison between albino rat model and pigmented rat model is seldom.Objective This study was to observe and compare the manifestations of FFA and retinal digest preparation in early diabetic pigmented diabetic rat and albino diabetic rat,and to select the right model of DR using FFA.Methods Type 1 diabetic models were induced in 15 six-week-old health male BN rats and 15 six-week-old health male SD rats by the injection of STZ (55 mg/kg) via tail vein.The models were thought to be successful in the rats with the blood glucose level ≥ 16.7 mmol/L.The right eyes of the rats were extracted 6 weeks after injection of STZ.Lens were examined by slit lamp microscope.Retinal vascular changes were examined by fundus photography,FFA and periodic acid Schiff staining of retinal digest preparation.The manifestations of FFA and retinal digest preparation were contrasted between BN rats and SD rats.The number of eyes with lens opacification was compared by Chi-square test and the ratio of vascular endothelial cells and perithelial cells (E/P) was compared between BN rats and SD rats.The use and care of experimental animals complied the Statement of Ethic Committee of Tianjin Medicine University.Results Six weeks after injection of STZ,11 BN rats and 10SD rats were included in this study.The blood levels were (24.73±2.98) mmol/L and (22.36±3.65) mmol/L in BN rats and SD rats,respectively,without significant difference between the 2 types of rats (t =7.873,P＞0.05).Lens opacification occurred in 6 BN rats and in 5 SD rats (P=0.717).FFA showed the clear retinal vascular under the brown background.Evident vessel disorder and fluorescence leakage like background stage of DR were seen in 9 eyes.However,in the SD rats,retinal vessel abnormality could not exhibited owing to the interference of choroid fluorescent light from choroidal vessels and vortex vein.Retinal digest preparation exhibited that unevenness of vessel diameter,decrease of perithelial cells and increase of endothelial cells in both types of rats.The E/P values were 11.50±3.68in the BN rats and 12.86±3.94 in the SD rats,without significant difference between them (t=9.785,P＞0.05).Conclusions The abnormality of fundus vascular morphology can be better displayed in pigmented diabetic rats than albino rats by FFA in vivo.

Objective To explore the pathogenic bacteria′s distribution and their drug resistance of urinary tract infection in children.Methods A total of 555 pathogen strains in urinary tract infection for children from inpatients and outpatients from Jan.2005 to Dec 2006 were identified and the drug resistance test was preformed.Results In the 555 strains of bacteria,80.7% were Gram-negative,17.7%were Gram-positive and 1.6%were fungi.Most of Gram-negative bacteria were E.coli,among 300 strains of identified Escherichia,69.3% of them(208 strains) produced extended speetrum ? lactamases(ESBLs);among 41 strains of identified Klebsiella pneumoniae,78.1% of them(32 strains) produced ESBLs.Most of Gram-positive bacteria were Enterococcus,there were 70 strains(12.6%),sensitivity rates of nitrofurantion and vancomycin were 100%.Conclusion Gram-negative is the main infection bacterium in urinary tract infection for children and the most of them are very serious drug resistant.The clinicians should pay more attentions to idstream urin bacteriology culture and choose the suitable antibiotic according to the results of antibiotic susceptibility tests.

Objective:To study the influence of Wnt5a gene silence on the proliferation,migration and invasion of lung squamous carcinoma cells.Methods:A recombinant plasmid pHI-siRNA~(Wnt5a-)was constructed and used to deliver small interference RNA (siRNA)targeting Wnt5a in SK-MES-1 cells;the transfected cells were screened to establish a stable transgenic cell line.MTT,cell cycle and Transwell assays were employed to evaluate the effect of Wnt5a gene silence on the proliferation,migration and invasion of lung squamous carcinoma cells.Results:Western blotting assay revealed that Wnt5a was lowly expressed in SK-MES-1~(Wnt5a-)(13.6%).The proliferation index(PI)of transgenic cell line was slightly lower than that of the control cell line([28.3?3.8]% vs[30.5?5.2]%). The migration and invasion capabilities of SK-MES-1~(Wnt5a-)cells were(47.3?9.2)% and(39.7?11.7)% of the control cells, respectively.Conclusion:Low Wnt5a expression can significantly inhibit the migration and invasion capabilities of SK-MES-1 cells, indicating that Wnt5a might be a potential target for gene therapy of lung squamous carcinoma.

Objective To observe the effects of ultrasound medium frequency electrotherapy on pain and content of enkephalins. Methods The pain threshold of the rat was measured before and after the application of ultrasound medium frequency electrotherapy. The content of met enhephalin(MEK) and leu enkephalin(LEK) in the glandula hypophysis and hypothalamus was also measured. A linear regression analysis based on the pain threshold and the content of enkaphalin was conducted to reveal the pain relieving mechanism of the ultrasound medium frequency electrotherapy. Results The level of MEK and LEK in the glandula hypophysis and hypothalamus was significantly increased after a single use action ultrasound medium frequency electrotherapy as compared with that before the treatment ( P