Adolescent ; Blood Glucose ; analysis ; Female ; Humans ; Kidney Transplantation ; Male ; Sodium ; blood ; Sodium Chloride ; administration & dosage

NF-?B, a group of important transcription factors, are introduced and discussed here on several aspects: their component and molecular structure; their activity control by I?B and IKK, the mechanism of their activation; their important roles in transcriptional regulation for large numbers of genes; and their importance in immunity, inflammation, cell survival, proliferation and apoptosis. The analysis of the relation between NF-?B and disease occurrence, the analysis of the relation between NF-?B and disease therapy, and the application prospect of the new strategy regarding the novel drug design and correlative diseases therapy on the basis of NF-?B as the target, are also included.

Human rhinoviruses (HRVs) are the causative pathogens in more than half of viral upper respiratory tract infections. Currently, no antiviral agents that are active against HRVs are available for clinical use. Because only higher primates are susceptible to HRVs, the screening of new drug is most commonly based on the cell line model. In this study, isolated rabbit airway smooth muscles (ASM) tissue model has been established, and the airway responsiveness with different treatment has been examined. Relative to control tissues, the maximal constrictor (Tmax) response to ACh increased significantly 150% in ASM inoculated with HRV, and relaxation to isoproterenol has been attenuated to 63%. And the abnormal responsiveness can be inhibited in presence of pretreatment with several new compounds which have been exhibited effective anti-HRV activity on cell lines. The results demonstrate that the established ASM model will be applied to screening the anti-HRVs drugs.

OBJECTIVE:To establish a RP-HPLC method for the determination of imperatorin in Qingwen jiedu tablets(heat-clearing and detoxifying agent).METHODS:The chromatographic separation was performed on Diamonsil-C18(250 mm?4.6 mm,5 ?m)with column temperature kept at 35 ℃.The mobile phase consisted of methanol-water(70∶30)at a flow rate of 0.8 mL?min-1 with detection wavelength set at 300 nm.RESULTS:The linear range of imperatorin was 2.7～27.0 ?g?mL-1(r=0.999 9)with its average recovery at 98.9%(RSD=1.44%,n=6).CONCLUSION:The established method was proved to be well-separated,sensitive,accurate and applicable for the quality control of Qingwen jiedu tablets.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Ankle Injuries ; therapy ; Female ; Humans ; Male ; Sprains and Strains ; therapy ; Treatment Outcome ; Young Adult

Humans ; Protein Kinase Inhibitors ; therapeutic use ; Protein-Tyrosine Kinases ; antagonists & inhibitors

Objective To investigate the characteristic immunophenotype of B cell chronic lymphoid leukemia in china. Method Single and multiparameter flow cytometry were used to analysis 163 cases of B cell chronic lymphoid leukemia. Results 71.8%(117/163) of cases co-expressed CD5 and B cell markers. The patients were classified into category of B cell chronic lymphocytic leukemia(B-CLL), hairy cell leukemia(HCL) and other B-cell lymphoproliferative disorders(LPDs) by using the scoring system that was recommended by world health organization (WHO). The B-CLL typically display the composite phenotypes: CD5+,CD23+,CD20+,CD19+,HLA-DR+,but the CD22,CD11c,CD25 and FMC7 were variable present in some B-CLL cases.CD103 seems the most specific marker for HCL.To differentiate diagnosis of atypical B-CLL with B-prolymphocytic leukemia(B-PLL) or mantle cell lymphoma(MCL), one must not rely exclusively on immunophenotypic dates, cytogenetic or molecular biology detection would be helpful. The index of froward scatter( FSC) and antigens expression of tumor B cells could be calculated by dividing the relevant value of residual normal T cell within same sample as internal control, so the cell size and the intensity of antigen expression could be comparable each other and quantitative between different investigations. Conclusion immunophenotypic analysis is an extremely useful adjunct in the diagnosis of chronic lymphoid leukemia.

Silent information regulator protein 1 (SlRT1) is one of the sirtuins and belongs to histone deacetyase. lts activity depends on nicotinamide adenine dinucleotide ( NAD+) and is regulated by NAD+. Experimental and clinical studies have shown the neuro-protective effect of SlRT1 in the pathogenesis of age-related eye diseases. ln this review, the relationship between SlRT1 activator and apoptosis in the retinal cells were discussed. The review also points to SlRT1 as a potential therapeutic target for the clinical management of age-related retinal disease.

Objective To explore the relationship between asthma exacerbation and respiratory tract infections of viruses,Mycoplasma pneumoniae(MP),Chlamydia pneumoniae(CP)in children.Methods Seven viruses including respiratory syncytial virus(RSV),adenovirus(AdV),influenza virus A(IFVA),influenza virus B(IFVB) and parainfluenza virus Ⅰ,Ⅱ,Ⅲ(PFVⅠ,Ⅱ,Ⅲ) from the nasopharyngeal aspirate of 74 patients with asthma were rapidly diagnosed by direct immunofluorescence assay,as well as the serum MP-IgM,CP-IgM were detected by the granule agglutinating method and indirect solid-phase enzyme immunoassay(EIA),respectively.Results Pathogens were detected in 29 out of 74 cases(39.2%) with asthma exacerbation.Of whom 14 cases(43.8%) were in infant and another 15 cases(18.8%) were in preschool and school children.RSV was the leading pathogen in infant,it was discovered in 6 cases(accounting for 18.8%).The se-cond pathogen was PFVⅢ which was discovered in 4 cases(12.5%).MP,AdV and CP accounted for 6.25%,3.1%,3.1%,respectively.But in preschool and school children,MP was the most common pathogen which were discovered in 9 cases(21.4%),the following pathogen was CP which was discovered in 3 cases(7.1%),PFVⅢ and RSV only accounted for 4.8%,2.4%,respectively.There was significant differences statistically between two groups in viral respiratory tract and atypical-microorganism infections rate(Pa

OBJECTIVE: To test the technical parameters of GlobalFiler® PCR Amplification Kit for its application to forensic application value and to investigate the genetic polymorphisms. METHODS: The validation was conducted in sensitivity, mixed samples, species specificity, adaptability, survivability, consistency, peak height balance and stability. The amplification and detection of the genomic DNA from 373 unrelated individuals from Beijing Han nationality were extracted by automation workstation. RESULTS: Global-Filer® PCR Amplification Kit was adaptive to some mixed, degraded and inhibited samples. The power of sensitivity and adaptability and peak height balance showed well. The distributions of genotype frequencies for 21 STR loci in the population were all in accordance with Hardy-Weinberg equilibrium (P > 0.05). The PIC value of the 21 STR loci was among 0.536 to 0.940; the H value was among 0.558 to 0.933; the DP value was among 0.783 to 0.992; the PE value was among 0.243 to 0.874. CONCLUSION GlobalFiler® PCR Amplification Kit is suitable for criminal cases and DNA database in forensic practice. And 21 STR loci in Beijing Han nationality have high polymorphism, which have application value in forensic practice and population genetics.
Asian People/genetics* ; Beijing ; Databases, Nucleic Acid ; Ethnicity ; Gene Frequency ; Genetic Loci/genetics* ; Genetics, Population ; Genotype ; Humans ; Polymerase Chain Reaction/standards* ; Polymorphism, Genetic ; Reproducibility of Results ; Species Specificity