1.Effect evaluation of application of intravenous indwelling needle and infusion connector joint in children undergoing chemotherapy
Chinese Journal of Practical Nursing 2011;27(34):35-36
Objective To discuss the advantages of combined use of intravenous indwelling needle and infusion connector joint.Methods 120 cases of children in our hospital receiving chemotherapy through intravenous infusion were randomized into the control group and the experimental group with 60 cases in each group.The control group used heparin cap,after each infusion was completed,with 12.SU/ml 3~5ml of heparin saline pulse-type sealing.The experimental group adopted infusion connector joint,after the infusion completed,3~5 ml normal saline was injected into the joint to flush the blessing sealed tube.The complications rate was calculated in two groups.Results The experimental group and the control group showed differences in the incidence of complications and the patients' approval to nurses.Conclusions Combination of intravenous indwelling needle and infusion connector joint can reduce the complications significantly,and improve patients' approval to nurses.
2.Effects of early enteral feeding containing medium and long-chain triglyceride on improving protein catabolism of burned patients
Parenteral & Enteral Nutrition 1997;0(02):-
Obfectives:The purpose of the present study was to compare the effects of different early enteral nutrition formula (containing either MCT/LCT or LCT only) on improving nutritional status of burned patients. Methods:This was a prospective,randomized,single blind and controlled study.Thirty cases of burned patients injured by varous causes with total burn surface area (TBSA) more than 30% were collected and randomized into two groups:①Group F:enteral feeding with Fresubin 750 MCT containing MCT/LCT as fat source,②Group N:enteral feeding with Nutrison,containing only LCT as fat source.All of the thirty patients received isocaloric,isonitrogenous,and isolipidic enteral feeding,with intragastric tube and enteral feeding pump,started within 24 h postburn.The several parameters were observed on postburn day(PBD) 1,4,7 and 10,such as serum concentrations of transferrin and prealbumin and plasma concentrations of prostaglandin E 2(PGE 2) and insulin.Nitrogen in urine per day and excretion of 3 methylhistidine in urine on day 1,7 and 10 postburn were determined. Results:The results showed that the patients of F group had an increased blood concentration of transferrin,prealbumin and insulin,and a decreased plasma concentration of PGE 2.Excretion of nitrogen and 3 methylhistidine in urine was also markedly reduced in patients fed with nutrients containing MCT/LCT compared with LCT. Conclusions :We have shown that an enteral diet containing MCT/LCT can improve the nutrition status of burned patients more effectively.
3.Effects of advanced glycation end -products on thioltransferase expression in human lens epithelial cells
International Eye Science 2015;(4):588-591
AIM:To observe the effects of advanced glycation end-products (AGEs) on thioltransferase (TTase) expression and activity in human lens epithelial cells.
METHODS: Human lens epithelial cells B3 ( HLE B3 ) were treated with 1. 5mg/mL AGEs - BSA as the experimental groups cultured by fetal bovine serum of volume fraction 10% dulbecco modified eagle medium ( DMEM) and bovine serum albumin ( BSA) was added at the same concentrations as the negative control. The level of reactive oxygen species ( ROS ) was evaluated. Cells were collected at 0, 1, 2, 3, 4d and total RNA or protein was extracted. TTase mRNA levels were detected by qRT-RCR. TTase expression was detected by Western blot and its activity was measured.
RESULTS: Compared with the control group, AGEs-BSA up-regulated the expression of ROS (P<0. 01), ROS content increased in a time-dependent manner. BSA had no effects on ROS expression. The expression of TTase increased after treatment with AGEs-BSA for 1d, peaked at 2d (nearly 5. 06-fold increase, P<0. 01), then decreased gradually. No change was observed between BSA and control group (P>0. 05). Similarly, TTase activity peaked at 3d (nearly 2. 01-fold increase, P<0. 01). Western blot test found that TTase protein expression was increased
gradually, starting from the 3d TTase expression was reflected that there was statistically significant difference compared with control group (P<0. 05).
CONCLUSION:AGEs-BSA significantly increases the production of ROS in human lens epithelial cells, and it then induces the oxidative stress which may promote the expression of TTase and enhances the activity of TTase.
4.Introduction to nurse-staffing ladder and compensation system in NHS UK
Chinese Journal of Practical Nursing 2015;31(11):844-847
Objective To explore ladder and compensation system of nurse-staffing which is suitable for the situation of our country.Methods The ladder management and compensation system to nursestaffing of National Health Service (NHS) in UK were given detailed introduction and analysis through the official documents,references and personal study experiences,and summarize its scientific and referenced aspects.Results NHS has formed a set of mature competency-based ladder system,and linked the ladder system to compensation system,made a relative scientific performance appraisal system,realized the scientific competency matches the level,salary changes along with the level model.Conclusions It provides references and thinking to the in-depth reform of public hospitals in our country,and the development of high quality nursing service,as well as the implementation of nursing ladder management and compensation reform.
5. A correlation study on magnetic resonance imaging features and nuclear DNA contents in meningiomas
Tumor 2008;28(8):694-698
Objective: To study the correlation of magnetic resonance imaging (MRI) features with the tumor nuclear DNA contents, and evaluate the biological behavior and proliferative potential of meningiomas using MRI. Methods: The DNA contents and the incidence of aneuploid of 45 patients with meningiomas were measured by means of photodensity method with image cytometry. The data were compared with MR findings for the statistical correlation analysis. Results: Tumors with the presence of peritumoral edema, irregular tumor shape, and ambiguous brain-tumor border had remarkable difference in DNA contents compared with those with negative peritumoral edema, smooth tumor shape, and clear brain-tumor border (P < 0.01). Using Spearman' s correlation coefficient analysis, we found a significant correlation (r = 0.696, P = 0.000) between three of MRI scores calculated for each patient and DNA contents. Conclusion: DNA contents and the incidence of aneuploid are higher in the tumors with the presence of peritumoral edema, irregular tumor shape, and ambiguous brain-tumor border. All of the imaging appearances can be used to estimate the proliferation potency and malignant biological behavior of the meningiomas.
6.Relationship between pre-pregnancy body mass index and preterm birth
Lifang WANG ; Hong ZHOU ; Yan ZHANG ; Yan WANG
Journal of Peking University(Health Sciences) 2016;48(3):414-417
Objective:To study the relationship between pre-pregnancy body mass index (BMI)and preterm birth.Methods:A case-control study was conducted in Haidian Maternal and Child Health Hos-pital in Beijing from January to April in 201 3.This study contained 1 74 preterm births in the case group and 382 term deliveries in the control group.The height,pre-pregnancy body weight,body weight before delivery,gestational weeks,history of diseases,family history of diseases,and complications during pregnancy of the subjects were collected.Multivariate Logistic regression was used to estimate the odds ratio and 95% confidence intervals (CI)after adjustment by maternal age,education,smoke during pregnancy,primiparous,mean income,and mean family living space.Results:After analyzing the rele-vant risk factors of preterm birth,the multivariate Logistic regression showed that pre-pregnancy obesity was a risk factor for preterm birth,the adjusted odds ratio was 2.461 (95% CI:1 .1 74 -5.1 59,P =0.01 7).The associations between pre-pregnancy overweight and preterm birth or pre-pregnancy under-weight and preterm birth were not found.The gestational diabetes mellitus,pregnancy-induced hyperten-sion,and family history of preterm birth were risk factors for preterm birth,the adjusted odds ratios were 1 .781 (95% CI:1 .025 -3.095,P =0.040),3.831 (95% CI:2.044 -7.1 80,P <0.001 ),and 3.675 (95% CI:1 .358 -9.942,P =0.01 0),respectively.Conclusion:Pre-pregnancy obesity ap-peared to be a risk factor for preterm birth.To decrease the incidence of preterm birth,women should improve preconception care and keep their BMI in a normal range before pregnancy.
8.Diagnostic tests for thyroid function and disorders.
Yan LIANG ; Hong WEI ; Mu-ti WANG
Chinese Journal of Pediatrics 2006;44(9):662-665
9.Functional examination of growth hormone-insulin-like growth factor axis in short stature children.
Hong WEI ; Yan LIANG ; Mu-ti WANG
Chinese Journal of Pediatrics 2005;43(2):99-103
Body Height
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Child
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Female
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Ghrelin
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pharmacology
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Growth Disorders
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physiopathology
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Growth Hormone-Releasing Hormone
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pharmacology
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Human Growth Hormone
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blood
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physiology
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urine
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Humans
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Insulin-Like Growth Factor Binding Protein 3
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blood
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Insulin-Like Growth Factor I
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physiology
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Male
10.Prognostic value of immunophenotype in patients with diffuse large B-cell lymphoma in the immune chemotherapy era
Yan LI ; Hong LIU ; Li AN ; Xiaomin WANG
Cancer Research and Clinic 2015;27(11):748-753
Objective To study the prognostic value of immunophenotypes in the patients with diffuse large B-cell lymphoma (DLBCL) and its prognostic significance of immunohistochemical markers for rituximab.Methods The data of 186 initial DLBCL patients were retrospectively analyzed.According to the international prognostic index,all patients were divided into relatively high-risk group and relatively low-risk groups,and the patients in both groups were divided into chemotherapy (CHOP) group and immune chemotherapy (R-CHOP) group,respectively,hnmunohistochemical method and Hans typing method were applied to immune classification in all of groups.The prognostic significances of the immunological subtypes in different prognosis group were compared by Log-rank test.Results The 5-year OS rate of the GCB subtype patients in the CHOP group was significantly higher than that of the non-GCB subtype (58.82 % vs 32.00 %,X2 =8.482,P=0.004),while that was no significant difference between GCB and non-GCB subtypes patients in the R-CHOP group (72.97 % vs 61.54 %,X2 =2.694,P =0.101).Further analysis results showed that non-GCB subtype patients treated with R-CHOP had significantly higher 5-year OS rate than those treated with CHOP (61.54 % vs 32.00 %,X2 =7.385,P=0.007).In the 107 cases of low risk group,there was no significant difference in 5-year OS rate between GCB and non-GCB subtype patients in the CHOP group or in the R-CHOP group (all P > 0.05),and there was no significant difference in 5-year OS rates between the CHOP and R-CHOP groups in the GCB group or in the non-GCB group (all P > 0.05).In the 79 cases of high risk group,the 5-year OS rate in the GCB subtype group was significantly higher than that in the non-GCB subtype in the CHOP group (40.00 % vs 22.72 %,X2 =3.978,P =0.045),while there was no significant difference in 5-year OS rates between GCB and non-GCB subtype patients in the R-CHOP group (62.50 % vs 42.31%,X2 =2.072,P =0.150).Conclusions By immunohistochemistry,DLBCL can be divided into two immunophenotype subgroups,which have guiding significance for early prognosis and therapeutic strategies,but which are no longer or weak in prognostic significance in the era of post-rituximab.R-CHOP improve significantly survival of non-GCB subgroup patients.However,its function needs to be further explored in the GCB subgroup.