1.Proteomic study of formalin-fixed and paraffin-embeded tissues.
Chinese Journal of Pathology 2009;38(10):718-720
2.Effects of Folic Acid on Human T Lymphoid Leukemia Cell Line CEM Cells
li-jun, CHEN ; tai-e, ZHANG ; hong-mei, WANG ; fei, GAO ; yan, GU
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To investigate the effects of the folic acid on human T lymphoid leukemia cell line CEM cells. Methods 1. MTT method was used to detect the proliferation of CEM cells co- cultured with folic acid of different concentrations and time;2. E-xamine the changes of morphology by light microscopy with Giemsa stain;3. Detect the percentage of apoptosis and cell cycle distribution as well as the expression of the apoptosis protein(Bcl- 2,C- myc) by flow cytometry;4. Detect DNA fragments by Agaiose elec-trophoresis;5. Detect the influence of folic acid to the anticancer effects of methotrexatc(MTX) by MTT methods. Results 1 Folic acid could inhibit the proliferation of CEM cells, and the optimal inhibitive concentrations range from 0. 4 ? 10-4 ?g/L to 3. 0 ? 10 -4 ?g/L,the inhibition rate was about 30% - 40% ; 2. Co - cultured with folic acid at 24,48, 72 hours, examined by light microscopy with Giemsa stain, apoptosis cells were found in all study groups but the higher apoptosis rate was found co - cultured with folic acid at concentration of (0.4 - 3.0) ? 10-4?g/L;3.The highest apoptosis rate was 6. 19% found at the concentration of 3 ? 10-4 ?g/L, but the cell cycle distribution had no statistical difference with control group, the expression of apoptosis related protein Bcl - 2 and C-myc was decreased;4 DNA was extracted from CEM cells co - cultured with 0.4? 10 -4 ?g/L and 3 ? 10-4 ?g/L folic acid for 48 hours. UNA ladders were visible by agarose electrophoresis of DNA fragments; 5. Folic acid did not affect the antitumor effect of MTX at the concentration from 0 2? 10-4 ?g/L to 12.0?10-4 ?g/L. Conclusion Folic acid may suppress proliferation and induce apoptosis of CEM cell
3.Normal sperm morphology and the outcomes of routine in vitro fertilization.
Bing HE ; Jun-ping CHENG ; Qi PAN ; Yan CHI ; Tai-shuai HUANG ; Xian-bao MAO ; Jie QIN ; Wei-hong TAN
National Journal of Andrology 2016;22(1):32-36
OBJECTIVETo explore the application value of morphology assessment of sperm from fresh semen in routine in vitro fertilization (IVF).
METHODSWe analyzed the morphology of the sperm from fresh or optimized semen samples and, based on the sperm morphology of the raw semen, allocated 908 IVF cycles due to the pure tubal factor to different groups: morphologically normal sperm (MNS) ≤ 4%, > 4% - ≤ 15%, and > 15% in Trial 1 and MNS ≤ 1%, > 1% - ≤ 2%, > 2% - ≤ 3%, and > 3%-- ≤ 4% in Trial 2. We compared the rates of fertilization, cleavage, high-quality embryo, -blastocyst formation, and pregnancy among different groups.
RESULTSThe total fertilization rate was significantly lower in the MNS ≤ 4% than in the MNS > 4% - ≤ 15% and >15% groups (74.40% vs 78.61% and 80.03%, P < 0.01). Compared with the MNS ≤ 1%, > 1% - ≤ 2%, and > 2% - ≤ 3% groups, the MNS > 3% - ≤ 4% group showed remarkably increased rates of 2PN normal fertilization (77.23%, 78.97% and 78.99% vs 85.47%, P < 0.01), cleavage (95.71%, 96.01% and 97.27% vs 98.73%, P < 0.05), and blastocyst formation (53.85%, 49.01% and 49.55% vs 63.41%, P < 0.01). No statistically significant differences were observed in the rates of clinical pregnancy, implantation, early abortion, live birth, or malformation at birth among different groups (P > 0.05).
CONCLUSIONMNS ≤ 4% affected the total rate of fertilization while MNS ≤ 3% reduced the rate of normal fertilization in IVF. However, even MNS ≤ 1% did not result in fertilization disorder or failure. Therefore, teratozoospermia alone was not an indicator of ICSI and sperm mor- phology assessment had no obvious value for predicting the rates of embryo quality, clinical pregnancy, and live birth in IVF.
Female ; Fertilization in Vitro ; Humans ; Male ; Pregnancy ; Pregnancy Outcome ; Spermatozoa ; cytology
4.Design and clinical application of a guiding instrument for anteversion of femoral neck.
Zhen-kui WANG ; Jie GAO ; Tai-an SUN ; Cheng-tao ZHU ; Hong-yan ZHAO
China Journal of Orthopaedics and Traumatology 2008;21(9):705-705
Adult
;
Aged
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Aged, 80 and over
;
Equipment Design
;
Female
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Femur Neck
;
surgery
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Fracture Fixation, Internal
;
instrumentation
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Humans
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Male
;
Middle Aged
5.Quantitative study of effects of cinnamaldehyde on levels of endotoxin in root canals in rats' periapical periodontitis model.
Hong-ying LIU ; Wen-yan LI ; Tai-guo HAO ; Hong-yan GAO ; Yue MA
West China Journal of Stomatology 2007;25(3):233-236
OBJECTIVETo provide experimental evidence for the exploitation of cinnamaldehyde as a kind of root canal disinfectant through studying the effect of cinnamaldehyde on endotoxin in root canals.
METHODSThis experimental model of periapical periodontitis was established with Wistar rats. The 75 rats were divided randomly into 3 groups: Group of cinnamaldehyde, group of formaldehyde cresol formocresol, group of physiological saline. The level of endotoxin was measured by quantitative chromogenic tachypleus amebocyte lysate method before and after sealing the drugs in the root canal.
RESULTSThe level of endotoxin in the group of cinnamaldehyde and formaldehyde cresol formocresol decreased obviously (P < 0.05), and the difference between them was of no significance (P > 0.05), the group of physiological saline was of no significant difference (P > 0.05).
CONCLUSIONCinnamaldehyde can decrease the level of endotoxin obviously.
Acrolein ; analogs & derivatives ; Animals ; Dental Pulp Cavity ; Endotoxins ; Formocresols ; Periapical Periodontitis ; Rats ; Rats, Wistar ; Root Canal Irrigants ; Root Canal Therapy
6.Histopathology research of Cinnamaldehyde as pulp-cap of pulpotomy in rats.
Hong-yan GAO ; Wen-yan LI ; Tai-guo HAO ; Hong-ying LIU
West China Journal of Stomatology 2007;25(5):429-431
OBJECTIVETo investigate the stimulation of Cinnamaldehyde to the pulp tissue and the periapical tissue of rats' teeth, to provide evidence for developing Cinnamaldehyde as a pulp-cap of pulpotomy in primary teeth.
METHODSUsing Cinnamaldehyde as pulp-cap in pulpotomy of rats' teeth, set up Cinnamaldehyde group, formaldehyde cresol formocresol group and blank group. After different treatment according to the empirical procedure, rats were killed in the 4th week and on the 12th week. Then the experiment teeth and the periodontal tissue were made into HE slides and observed using light microscope.
RESULTSThe 4" week, internal absorption, external absorption, and calcification were of no significance among all the groups. Inflammation in blank group was far more severe than that in other two groups (P<0.05), while the latter two were of no significance. The 12th week, internal absorption, external absorption, and inflammation between the former two groups were of no differences, but the indexes were significantly different from them of the blank group (P<0.005). Calcify was not obvious in all the slides.
CONCLUSIONAs a pulp-cap, Cinnamaldehyde stimulates the pulp tissue and the periapical tissue at a very low level. The research provides histopathology rationale for Cinnamaldehyde as pulp-cap of pulpotomy in primary teeth.
Acrolein ; analogs & derivatives ; Animals ; Dental Pulp ; Formocresols ; Pulpotomy ; Rats ; Tooth, Deciduous
7.Severe liver injury induced by repeated use of hair dye.
Feng-Qin HOU ; Xiao-Hong LIN ; Yan-Yan YU ; Tai-Ling WANG ; Gui-Qiang WANG
Chinese Medical Journal 2009;122(7):875-877
8.Research progress and literature review of animal models of osteoporosis
Yan-Hong LI ; Xiao-Qian DANG ; Fu-Tai GONG ; Wen-Rui BAN ; Jun MA ; Yao-Wu SHI ; Hong-Lei ZHANG
Chinese Journal of Tissue Engineering Research 2018;22(12):1956-1961
BACKGROUND: Osteoporosis, known as "a quiet epidemic disease", is an "invisible killer" that impairs the health of the elderly. Fracture as the most dangerous complication of osteoporosis is not only a medical problem, but also a serious social problem. OBJECTIVE: To summarize the selection, establishment methods, characters and evaluation of the animal model of osteoporosis. METHODS: A computed-based search of CBM, WanFang, VIP, CNKI and PubMed databases was conducted for the relevant articles published from January 1986 to December 2016 using the keywords of "osteoporosis, animal models" in English and Chinese, respectively. Finally 39 articles were included for result analysis after reading the title and abstract. RESULTS AND CONCLUSION: Animal experiments play an important role in the studies of osteoporosis, so a desirable animal model should hold similarity, reliability, repeatability, applicability, controllability, efficacy and economy. Osteoporosis is a progressive and long-lasting disease that has different characteristics at different stages. But an animal model only simulates one etiology, one stage, major symptoms and pathophysiological changes, and cannot exhibit the whole pathological changes. The current study suggests that animal species and modeling methods can affect the characters of models, and there is a lack of standards for animal age, feeding methods, modeling time and assessment.
9.Application of intelligent consumables management belt in ward unit of emergency medical team
Tai-Hong GUAN ; Lei ZHAO ; Yan LIU ; Hong-Min LIU ; Yi LI
Chinese Medical Equipment Journal 2023;44(12):69-73
The current situation and problems of the consumables management in the ward unit of the emergency medical team were introduced.An intelligent consumables management belt was prepared based on two-dimensional code technology to solve the problems of the ward unit in consumables positioning,storage,inconvenient access,management process and support cost,which enhanced the efficacy of the emergency medical team greatly.
10.The infection status of anisakid larvae in marine fish and cephalopods from the Bohai Sea, China and their taxonomical consideration.
Hong Wei MA ; Tai Jing JIANG ; Fu Shi QUAN ; Xiao Guang CHEN ; Hui dong WANG ; Yun Shu ZHANG ; Ming Shan CUI ; Wen Yan ZHI ; Dian Chen JIANG
The Korean Journal of Parasitology 1997;35(1):19-24
The infection status of anisakid larvae was examined in 290 marine fish of 25 species and in 108 cephalopods of 3 species purchased in Bayuquan region, Yingko city nearby the coast of the Bohai Sea from may to August 1992. A total of 7,327 larvae were collected from 156 fish of 19 species and 8 squids of one species. The 3rd-stage larvae of Anisakis simplex were collected from 121 fish (63.4%) of 15 species (N = 191) and from 8 squids (14.8%) of one species (N = 54), and they were total, 5,992 (81.8%). Out of remaining 1,335 larvae, 154 (2.1%) were classified as Thynnascaris type B from 23 fish of 4 species, 1,013 (13.8%) as Thynnascaris type C from 79 fish of 13 species. 164 (2.2%) as Hysterothylacium China type V from 20 fish of 4 species, 3 (0.04%) as Raphidascaris from 3 fish of 2 species and one was Pseudoterranova decipiens larva.
Animal
;
Anisakiasis/veterinary*
;
Anisakiasis/parasitology
;
Anisakiasis/epidemiology
;
Anisakis/isolation & purification
;
Anisakis/classification*
;
China
;
Fish Diseases/parasitology*
;
Fish Diseases/epidemiology
;
Fishes
;
Larva
;
Seawater
;
Squid/parasitology*