3.Effects of enamel matrix proteins on the proliferation and protein synthesis of ectomesenchymal stem cells in vitro
Feng CHAI ; Yan JIN ; Yimin ZHAO
Journal of Practical Stomatology 2001;0(01):-
Objective: To investigate the effects of enamel matrix pr ot eins(EMPs) and purified EMPs(EMD) on the proliferation and protein synthesis act ivity of rat ectomesenchymal stem cells in vitro. Methods: C ell culture technique and 3H-leucine label assay were used to measure the pr oliferation and protein synthesis activity of rat ectomesenchymal stem cells exp osed to the EMPs- or EMD-conditioned culture media with different concentratio n. Results: Both EMPs and EMD at 50~200 mg/ml increased the pro l iferation and protein synthesis of the cells in 10-day-culture. EMPs and EMD a t 150 mg/ml showed the strongest effects(P
4.On Cultivating Teamwork among Medical Students
Huimin LIANG ; Wei ZHAO ; Feng ZHAO ; Bing YAN
Chinese Medical Ethics 1996;0(01):-
Modern medical staff are facing the challenge to survive in competitions and develop through cooperation.Thus it is of great importance for the future medical staff,that is,medical students to cultivate and enhance their teamwork for future demand.Cultivating approaches mainly include creating a collaborative environment,carrying out cooperative scientific research,creating teamwork in the curriculum,and launching community service activities.
5.A possible mechanism linking hyperglycemia and reduced high-density lipoprotein cholesterol levels in diabetes.
Feng, GAO ; Tong, YAN ; Yan, ZHAO ; Fan, YIN ; Cuining, HU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(3):318-21
This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol (HDL-C). Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco's modified Eagle's medium (DMEM) containing glucose of various concentrations. The cells were divided into 3 groups in terms of different glucose concentrations in the cultures: Control group (5.6 mmol/L glucose), high glucose concentration groups (16.7 mmol/L and 30 mmol/L glucose). ATP-binding cassette transporter A1 (ABCA1) mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24, 48 and 72 h after glucose treatment. The results showed that ABCA1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points (P>0.05 for each). In the 30 mmol/L glucose group, macrophage ABCA1 mRNA expression was not changed significantly at 24 h (P=0.14), but was substantially decreased by 40.4% at 48 h (P=0.009) and by 48.1% at 72 h (P=0.015) as compared with that in the control group. It was concluded that ABCA1 is of vital importance for HDL-C biogenesis. High glucose may hamper HDL-C biogenesis by decreasing ABCA1 expression, which contributes to low HDL-C level in diabetes.
6.Antibody to Ro-52 in patients with autoimmune liver disease
Dantong ZHAO ; Huiping YAN ; Yufen TAN ; Yan LIU ; Yan ZHAO ; Xia FENG ; Daijun XIANG
Chinese Journal of Microbiology and Immunology 2009;29(7):656-659
Objective To investigate the significance of antibody to Ro-52 in patients with autoim-mune liver disease(AILD). Methods One hundred and fifteen patients with abnormal liver functions, who had anti-Ro-52 detection by immunological blotting, were reviewed retrospectively. According to types of AILD, the clinical features were compared between patients with and without anti-Ro-52, respectively, κ test of concordance was used to provide a chance-corrected valve for immune-serological results. Results The rates of anti-Ro-52 in autoimmune hepatitis( AIH), primary sclerosing cholangitis(PBC) and AIH/PBC o-verlap syndrome groups were 32.43%, 24.56% and 33.33%, respectively, there were no significant differ-enees among three groups ( x2 = 0. 949, P >0. 05). The rate of anti-soluble hver antigen/liver-pancreas ( an-ti-SLA/LP) in AIH patients with anti-Ro-52 (58.33%) was higher than AIH patients without anti-Ro-52 ( 16.00% ,P < 0.05 ). The rate of anti-SLA/LP in AIH/PBC overlap syndrome patients with anti-Ro-52 (85.71%) was also higher than that of control group (28.57% ,P <0.05). Anti-Ro-52 and anti-SLA/LP had concordance according to κ test( κ >0.40, P <0.05). The average level of IgG in AIH/PBC overlap syndrome patients with anti-Ro-52 was higher than patients without anti-Ro-52 ( t = 2. 508, P < 0.05 ). Conclusion The rates of anti-Ro-52 in AIH, PBC and AIH/PBC overlap syndrome were of no significant differences. Anti-Ro-52 may have correlation with anti-SLA/LP. AIH/PBC overlap syndrome patients with anti-Ro-52 shewed higher IgG level than patients without anti-Ro-52.
7.Cloning of the Coding Region and 3′Non-coding Region of Calmodulin 2 in Guinea Pig
Rui FENG ; Yan LIU ; Lei YANG ; Huiyuan HU ; Feng GUO ; Meimi ZHAO ; Jinsheng ZHAO ; Liying HAO
Journal of China Medical University 2015;(2):123-126
Objective To clone the coding region and 3′non?coding region of calmodulin 2(CaM2)in guinea pig,to provide the genetic informa?tion for studying the gene function of Calmodulin 2. Methods Total RNA was extracted from heart tissue of guinea pig,the coding region and 3′non?coding region of CaM2 were amplified by RT?PCR and 3′?RACE PCR methods,and the recombinant plasmid was constructed by inserting cDNA of the coding region and 3′non?coding region of CaM2 into the cloning vector by genetic engineering technology followed by DNA sequencing and se?quence analysis. Results The cloned coding region of CaM2 was 450 bp,and the 3′non?coding region of CaM2 was 660 bp. The amino acid se?quences of the coding region of CaM2 was consistent with those of other CaM subtypes,and the 3′non?coding region of CaM2 had low homology with those of other subtypes. Conclusion The cloning of CaM2 coding region and 3′non?coding region in guinea pig was the foundation for further study on the gene function of CaM2 and its role in related diseases.
8.Application of diffusion-weighted imaging in the diagnosis and treatment of cervical cancer
Chun FU ; Xiaoyan FENG ; Xiangquan WANG ; Yan ZHAO
Journal of International Oncology 2012;39(6):449-452
Diffusion-weighted imaging (DWI) is sensitive to identification of cervical lesions and lymph node metastasis.DWI can be used to predict and evaluate the therapeutic effect of cervical cancer.Using magnetic resonance imaging and DWI scans in the process of diagnosis and treatment of cervical cancer,which may contribute to the personalized treatment program and improve prognosis for patients.
9.The comparative analysis of survival rate and etoposide plus cisplatin treatment outcome in small-cell lung cancer patients with different hyponatremia
Liang ZHAO ; Jinglu SHAN ; Yan FENG ; Nan DAI ; Dong WANG
Chongqing Medicine 2016;45(27):3814-3816
Objective to investigate the outcome and EP treatment outcome of small‐cell lung cancer (SCLC) patients with different hyponatraemia .Methods This retrospective study analyzed the relationship between the serum sodium ,serum osmolality , urine sodium ,urine osmolality and survival time of 51 patients .Moreover ,we analyzed the survival time and chemotherapy outcome of SCLC patients in hypovolaemic and euvolaemic hyponatraemia .Results The data indicated that the serum sodium and osmolality correlated with the survival time positively ,and the pearson correlation coefficient are 0 .48 [95% CI:(0 .23 to 0 .67)]and 0 .61 [95% CI:(0 .40 to 0 .76)] ,respectively .urine sodium and osmolality correlated with survival time negatively ,and the pearson corre‐lation coefficient are -0 .6 [95% CI:(-0 .75 to -0 .38)] and‐0 .31 [95% CI:(-0 .54 to -0 .04)] ,respectively .Etoposide plus cisplatin treatment showed less effectiveness to the SCLC patients in euvolaemic hyponatraemia (29 .17% VS .66 .7% ,P<0 .05) , and the survival time of SCLC patients in euvolaemic hyponatraemia is shorter (33 .3% VS .92 .6% ,P<0 .05) .Conclusion Euvol‐aemic hyponatraemia could be a risk factor for poor outcome in SCLC .
10.Effects of patient-related and stroke-related factors on post-stroke aphasia recovery
Yanlong ZHAO ; Zhongrui YAN ; Zuoji ZHANG ; Feng MEI ; Shuyin SUN
Chinese Journal of Behavioral Medicine and Brain Science 2016;25(7):667-670
Post-stroke aphasia is an acquired language disorder caused by stroke.It impaired the quality of life of patients and brought a heavy burden to family and the society.Unfortunately,the highly variable predictive factors make the prognosis of aphasia recovery difficult.Much of the researches indicated that the post-stroke aphasia recovery is associated with the patient-related and stroke-related factors.We searched the recent advances on the influence of patient-related factors and stroke-related factors on aphasia recovery.It showed that patient-related factors have no obvious effect on predicting aphasia recovery while the lesion (stroke)-related factors appeared close correlation with post-stroke aphasia recovery.The clinicians should pay more attention on lesion (stroke)-related factors when evaluate the outcome and give the intervention measures.