Objective To analyze projects funded by NSFC and SCI paper publication in orthopedic research field (Code:H06) from 2010 to 2016,the current research status and development trend in this field were reviewed and discussed.Methods The data of the projects and funding subsidized by NSFC in orthopedic research field from 2010 to 2016 were collected and analyzed;the papers published were collected by searching in the Web of Science core collection by orthopedics as keyword (article included,conference abstract and book chapters excluded;data collected by December 25,2016) and compared for analyzing the global and China's SCI papers publication in orthopedic field and the role of NSFC in it.Results During the period of 2010 to 2016,1603 projects were funded by NSFC in orthopedic field and the total subsidy fund reached ￥ 730 850 000 (direct funding reported in 2015 and 2016);821 projects of General Program were funded and subsidy fund amount to ￥481 490 000;567 projects of Young Scientists were funded ￥ 118 130 000;130 projects of Fund for Less Developed Regions were funded ￥ 56 040 000;12 projects of Key Program were funded ￥ 33 560 000;3 projects of Distinguished Young Scholars were funded ￥ 7 500 000;5 projects of Outstanding Young Scholars were funded and funding up to ￥ 5 900 000.In the past seven years,the volume of SCI papers published in the world exceed 1 million per year and the papers from the orthopedics field accounted for 0.7％ to 0.8％,ranked 54.The number of SCI papers published by Chinese institutions in the field increased year by year and country ranking climbed to No.3 in 2016 from No.8 in 2010.Among these SCI papers from Chinese institutions,the number of the papers from NSFC grants also increased year by year,ranked Top One in Chinese founding agency for 7 consecutive years.Conclusion In recent years,with the increasing of NSFC budget,the orthopedic research has been developed rapidly,but it still needs to be further strengthened in the advanced talents and high-level research.

Adult ; Cranial Nerve Neoplasms ; diagnosis ; Diagnostic Errors ; Facial Nerve ; pathology ; Hemangioma ; diagnosis ; Humans ; Male

0.2 g/L)with normal diets. Conclusions Living donor liver transplantation for hepatic complications of Wilson's disease can cure and correct the underlying metabolic defect. It is a lifesaving therapy in children with fulminant Wilsonian hepatitis and has many unsurpassed advantages.

Objective To evaluate the therapeutic effects and dose-effect relationships of different doses of the blood-cooling and blood flow-promoting drugs(凉血活血方) on radiation-induced lung injury of rat.Methods Seventy-two Wistar rats were randomly allocated into four groups: irradiation group(group A),small-dose group(group B,9 g?kg-1?d-1),middle-dose group(group C,18 g?kg-1?d-1) and high-dose group(group D,36 g?kg-1?d-1).All groups were repeatedly exposed to small dose of X-ray in the right hemi-thorax,and then the mice were sacrificed at different time points.The living animal features,the macro-changes of lung were observed,and pulmonary histopathological changes in all the groups were investigated,and the results of observation were compared.Results The symptoms of red patches around the nose and dry stool in groups C and D were less than those of groups A and B.After 26 weeks,lung coefficient in groups B,C and D was the same as that in group A,but right lung wet weights and lung coefficients at each time point in groups B,C and D were significantly lower than those in group A,and during 5th week,the right lung wet weights in groups C and D were obviously lower than the weight in group B(all P

Objective To investigate the change of serum transforming growth factor(TGF)-?1 and vascular endothelial growth facter( VEGF) in children with different types of primary nephrotic syndrome( PNS). Methods Children involved in the experiments were divided into simple type group, 16 cases; nephrit was type group, 16 cases, collecting blood sample in prednison - pretreated stage and in prednison- treated stage;control group, 14 cases. Monoclonal EUISA detected TGF-?1 and VEGF. Results Serum level of TGF-?1 and VKGF in active stage of simple type were higher than those of remission stage. The level in nephritis type was no signifi cant difference between prednison- treated stage and prednison - pretreated stage. The level in nephritis type was significantly higher than that in simple type. Conclusion Monitoring the dynamic change of serum TGF-?1 and VEGF can assess the effect of prednison treatment,and evaluate the prognosis of nephrotic syndrome.

ObjectiveTo study the protective effects of tumor necrosis factor-α(TNF-α) antibody on pancreatic encephalopathy in rats. MethodsSixty SD rats were randomly divided into the normal control group, acute necrotizing pancreatitis induction group and TNF-α antibody treated group. Acute hemorrhage necrotizing pancreatitis model in rats were induced by retrograde injection of 5% sodium taurocholate into the pancreatobiliary duct. Serum TNF-α was detected and animals were killed 12 h after drug administration. The changes of brain water contents, leucocyte accumulation and adhesion were measured, and pathological studies of pancreas and brain were performed. ResultsIn group of TNF-α antibody treated, serum TNF-α level decreased, brain water contents and leucocytes accumulation and adhension decreased significantly than that of acute necrotizing pancreatitis induction group (P<0.05). The histopathological change of pancrea was alleviative. ConclusionTNF-α antibody can alleviate the brain damage in acute hemorrhage necrotizing pancreatitis.

Background:Glioblastoma is one of the most common malignant brain tumors.Conventional clinical treatment of glioblastoma is not sufficient,and the molecular mechanism underlying the initiation and development of this disease remains unclear.Our study aimed to explore the expression and function of miR-873a-5p in glioblastoma and related molecular mechanism.Methods:We analyzed the most dysregulated microRNAs from the Gene Expression Omnibus (GEO) database and examined the expression of miR-873-5p in 20 glioblastoma tissues compared with ten normal brain tissues collected in the Zhejiang Tongde Hospital.We then overexpressed or inhibited miR-873-5p expression in U87 glioblastoma cell lines and analyzed the phenotype using the cell counting kit-8 assay,wound healing assay,and apoptosis.In addition,we predicted upstream and downstream genes of miR-873-5p in glioblastoma using bioinformatics analysis and tested our hypothesis in U87 cells using the luciferase reporter gene assay and Western blotting assay.The differences between two groups were analyzed by Student's t test.The Kruskal-Wallis test was used for the comparison of multiple groups.A P ＜ 0.05 was considered to be significant.Results:The miR-873-5p was downregulated in glioblastoma tissues compared with that in normal brain tissues (normal vs.tumor,0.762 ± 0.231 vs.0.378 ± 0.114,t =4.540,P ＜ 0.01).Overexpression of miR-873-5p inhibited cell growth (t =6.095,P ＜ 0.01) and migration (t=3.142,P ＜ 0.01) and promoted cell apoptosis (t=4.861,P ＜ 0.01),while inhibition of miR-873-5p had the opposite effect.Mechanistically,the long non-coding RNA HOTAIRM1 was found to act as a sponge of miR-873-5p to activate ZEB2 expression in U87 cells.Conclusions:We uncovered a novel HOTAIRM1/miR-873-5p/ZEB2 axis in glioblastoma cells,providing new insight into glioblastoma progression and a theoretical basis for the treatment of glioblastoma.

The total quantity control of pollutant emissions for an industrial district is determined by coefficient (A) method (Qian, 1990). It is suggested that average daily concentrations of air pollutants should be estimated in relation with metrological parameters, such as wind directions, wind speed and atmospheric stability in the period of monitoring by Gaussian model (SEPB, 1991), and that the sources of pollution should be redistributed on the basis of the result of monitoring with a view improving local atmosphere environment.

METHODS:A computer-based online research of PubMed, CNKI and VIP databases was performed with the key words of “epigenetic; inflammatory bowel disease; Crohn’s disease; ulcerative colitis; DNA methylation; histone modification; miRNA; moxibustion” in Chinese and English. RESULTS AND CONCLUSION:The correlation of epigenetic modification with inflammatory bowel disease occurrence and development is elaborated. The mechanisms in inflammatory bowel disease have been related from DNA methylation, histone modifications and miRNA targets. The mechanism of moxibustion on inflammatory bowel disease is primarily associated with immune regulation, and its anti-inflammatory mechanism may be affected by epigenetic regulation of inflammatory cytokines.

L-sorbose/L-sorbosone dehydrogenase from Ketogulonigenium vulgare S2 can transform L-sorbose to 2-KLG, which is widely used in production of Vitamin C. In order to obtain the engineering strain producing L-sorbose/L-sorbosone dehydrogenase and simplify the fermentation technology, firstly, this enzyme was purified by the methods of ammonium sulfate precipitation, DEAE Sepharose Fast Flow and Q Sepharose High Performance. Then, the purified L-sorbose/L-sorbosone dehydrogenase was injected to rabbit to obtain antibody. Next, the genomic library of Ketogulonigenium vulgare S2 was constructed by inserting the restriction fragments of chromatosomal DNA digested with Sau3A I into cosmid pKC505 vector digested by Hpa I and Pst I, which were packed with lamda phage package protein and transferred into E. coli DH5alpha in vitro. Finally, the positive strain K719# was selected from more than 12,000 clones via Dot-ELISA. Through the test of SDS-PAGE and thin layer chromatography, the results showed that the engineering strain K719# had the same biological activity as Ketogulonigenium vulgare S2 after adding coenzyme PQQ.
Carbohydrate Dehydrogenases ; genetics ; isolation & purification ; metabolism ; Cloning, Molecular ; Escherichia coli ; genetics ; metabolism ; Genomic Library ; Gluconobacter oxydans ; enzymology ; genetics ; growth & development ; Sorbose ; metabolism ; Sugar Acids ; metabolism ; Transformation, Bacterial