1.Food matching based on herbal properties of formulas in "Treatise on Febrile Diseases".
Su-rong YAN ; Hu-zhan ZHENG ; Su-fen MIAO ; Yun WANG
China Journal of Chinese Materia Medica 2015;40(18):3664-3666
Based on databases for herbal properties of formulas and foods recorded in "Treatise on Febrile Diseases", a case study was conducted for the food matching method according to herbal properties of formulas in "Treatise on Febrile Diseases". The result show that the method was technically feasible once the herbal properties of foods were determined. Moreover, according to herbal properties of target formulas, the compositions of foods were effectively defined. In this study, researchers determined the similarity between the food matching scheme and the target formulas in function and efficacy, provided a quantitative method for food formulation and promote the development of application technology of the herbal property theory and the compatibility theory.
Books
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history
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China
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Diet Therapy
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history
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Drugs, Chinese Herbal
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chemistry
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history
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metabolism
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Food
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history
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History, Ancient
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Medicine in Literature
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Plants, Medicinal
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chemistry
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metabolism
2.Study in correlation of anxiety and depression condition with social support of patients with pulmonary hypertension
Fen GU ; Min HU ; Juan YAO ; Yan ZHU ; Yijun LU ; Xin JIANG ; Zhicheng JING
Chinese Journal of Practical Nursing 2011;27(14):20-23
Objective To investigate the effects of social support intervention on anxiety and depression of pulmonary hypertension(PH)patients,then provide a scientific basis for nursing of patients with pulmonary hypertension.Methods The general condition of PH questionnaire,self-rating anxiety scale (SAS),self-rating depression scale(SDS),social support rating scale(SSRS)were distributed to 131 patients with PH.Then make statistical analysis of patients'anxiety,depression and social support conditions.Results The score of anxiety and depression psychological conditions of patients with pulmonary hypertension was significantly higher than normal population,the difference wag statistically significant.Among 131 patients,16 patients with anxiety,accounting for 12.21% ;21 cases of patients with depression,accounting for 16.03% .28 patients at a high level of social support,92 patients at a medium level of social support,11 patients at a low level of social support,a total of 91.60% of the patients in the middle and higher levels of social support.Anxiety and depression scores had significant negative correlation with social support,objective support points and subjective support points.The anxiety,depression difference among different types of pulmonary hypertension was statistically significant.The difference of anxiety and depression scores between patients with idiopathic pulmonary arterial hypertension and patients with pulmonary hyper tension caused by congenital heart disease were significant.The depression scores between pulmonary hy pertension caused by pulmonary veno-occlusive disease and congenital heart disease were significantly different.Conclusions When nurses care pulmonary hypertension patients.those with different types of PH should be given targeted social support.Attention should be paid to transfer the source of social support to help them adopt a positive attitude to face the diseabe,then improve the treatment and care compliance of patients.
3.Mapping of BRCT1 domain of BRCA1 with chromatin unfolding activity.
Qi-Nong YE ; Yan-Fen HU ; Hong-Jun ZHONG ; Rong LI ; Cui-Fen HUANG
Chinese Journal of Biotechnology 2002;18(6):656-661
Breast cancer susceptibility gene 1(BRCA1) plays an important role in breast cancer development and progression. BRCA1 encodes a 1863-amino acid protein with two BRCA1 C-terminal (BRCT) domains at its C-terminus, BRCT1 and BRCT2. Many cancer-predisposing mutations are located in the BRCT domains, which have been shown to induce chromatin unfolding by use of an approach that allows visualization of large-scale chromatin structure through lac repressor/lac operator recognition. To map the important region of BRCT domain (amino acid residues 1642-1736), six deletion mutant constructs were made. The chromatin structure assay showed that amino acid residues 1691-1721 are involved in the induction of chromatin unfolding. To further localize the critical amino acid residues, ten alanine scanning mutant constructs were made. The chromatin structure assay demonstrated that the 1707IAGGK1711 region is critical for the chromatin unfolding activity. Based on the mapped important region, Blast analysis identified a novel homologous protein. Mapping of the BRCT1 domain may aid in the presymptomatic risk assessment and provide a valuable tool for further study on the BRCT1 structure and function.
BRCA1 Protein
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chemistry
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physiology
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Base Sequence
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Chromatin
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chemistry
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Cloning, Molecular
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Female
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Genes, BRCA1
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Humans
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Molecular Sequence Data
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Mutation
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Protein Folding
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Structure-Activity Relationship
4.Basal levels for gonadotropins in 318 children from birth to 6 years of age.
Zuang-jian XU ; Yu HU ; Gui-zhen WANG ; Yao-ming WANG ; Li-fen MAO ; Jing-yue ZHOU ; Xu-yan CHEN
Chinese Journal of Pediatrics 2003;41(2):148-148
Child
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Child, Preschool
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Female
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Follicle Stimulating Hormone
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blood
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Gonadotropins
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blood
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Humans
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Infant
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Infant, Newborn
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Male
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Sex Factors
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Time Factors
5.Safety management of 15 patients with severe pulmonary arterial hypertension treated by aerosolized iloprost
Fen GU ; Min HU ; Juan YAO ; Ying YU ; Xin JIANG ; Xiaoxia YAN ; Jie CHENG ; Xiqi XU ; Zhicheng JING
Chinese Journal of Nursing 2010;45(5):425-426
This paper summarizes the key points of safety management for 15 patients with severe pulmonary arterial hypertension treated by aerosolized iloprost. All patients achieved significant improvements and none of them suffered any severe side effect. Complete safety management during the therapeutic procedure improved the patients' treatment confidence and compliance,and thereafter strengthened the efficacy of treatment.
6.Etiological analysis of 1977 patients with acute liver failure, subacute liver failure and acute-on-chronic liver failure.
Xiao-yan LIU ; Jin-hua HU ; Hui-fen WANG ; Ju-mei CHEN
Chinese Journal of Hepatology 2008;16(10):772-775
OBJECTIVETo investigate the etiology of 1977 patients from northern China with acute (ALF), sub-acute (SALF) or acute-on-chronic liver (ACLF) failures.
METHODThe age, gender, etiology, pathogenesis, and prognosis of the 1977 patients with liver failures were retrospectively analyzed.
RESULTSOf the 1977 cases, the three most common causes of ALF were HEV (33.96%) or HBV (13.21%) infections or those caused by medicines (9.43%). The three predominant causes of SALF were medicines (31.53%), HEV (16.22%) or HBV (9.91%) infections, but those of the ACLF were HBV (90.29%) infection, alcoholic hepatopathy (2.65%), and HBV super infected with HEV (2.26%) infections. 90.09% (1781) patients were infected by hepatotropic viruses. Of these 1781 patients, the most common cause of their liver failures was HBV infection (92.93%). In these HBV infected patients, 77.10% were from 26 to 55 years old. From 2005 to 2007, there were 39 patients with alcoholic liver failure. In the past two years, there were 23 patients with drug induced liver failure. The improvement rate of the 1977 patients after their treatments was 35.56%. The improvement rate of HEV infected liver failure was higher than drug induced liver failure (P less than 0.05); no statistical significance was found between other groups (P more than 0.05).
CONCLUSIONDifferent types of liver failure have different predominant causes. HBV infection is the most common cause in our 1977 patients. In the past two years, the number of drug induced liver failures and alcoholic liver failures have been increasing.
Acute Disease ; Adult ; Chemical and Drug Induced Liver Injury, Chronic ; etiology ; Chronic Disease ; Female ; Hepatitis B ; complications ; Hepatitis E ; complications ; Humans ; Liver Diseases, Alcoholic ; etiology ; Liver Failure ; chemically induced ; classification ; etiology ; virology ; Male ; Middle Aged ; Prognosis ; Retrospective Studies
7.Clinicopathologic study of aortic valves in children.
Ping HUANG ; Hong-wei WANG ; Zhen-lu ZHANG ; Xiu-fen HU ; Yan-ping LI ; Pei-xuan CHENG ; Jian-ying LIU
Chinese Journal of Pathology 2006;35(10):623-624
Adolescent
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Aortic Valve
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abnormalities
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Aortic Valve Insufficiency
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complications
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pathology
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surgery
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Aortic Valve Stenosis
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complications
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pathology
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surgery
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Child
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Endocarditis
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complications
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pathology
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surgery
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Female
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Heart Defects, Congenital
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complications
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pathology
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surgery
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Heart Valve Prosthesis Implantation
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Humans
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Male
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Rheumatic Heart Disease
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complications
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pathology
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surgery
8.Analysis of prognostic factors for patients with acute-on-chronic liver failure.
Xiao-yan LIU ; Jin-hua HU ; Hui-fen WANG
Chinese Journal of Hepatology 2009;17(8):607-610
OBJECTIVETo analyze the prognostic factors for patients with acute-on-chronic liver failure, and to build a scoring system for assessment of the prognosis of liver failure.
METHODS480 patients with acute-on-chronic liver failure in our hospital from January 2006 to June 2008 were enrolled in this study. The patients were divided into improved group and deteriorated group. The clinical data were analyzed by using chi square test, independent-Samples T Test and Binary logistic regression.
RESULTSThe factors that significantly affected the prognosis of Acute-on-chronic Liver Failure included age, hepatitis or liver cirrhosis, Staging, Hyponatremias, alpha-fetoprotein (AFP), the prothrombin time activity (PTA), total bilirubin (TBil), creatinine (Cr), albumin (ALB) and Hepatic encephalopathy, ascites, alimentary tract hemorrhage (P less than 0.05, P less than 0.01). PTA, Hyponatremias, hepatitis or liver cirrhosis, Hepatic encephalopathy and alimentary tract hemorrhage were independent risk factors of prognosis.
CONCLUSIONPTA, Hyponatremias, hepatitis or liver cirrhosis, Hepatic encephalopathy and alimentary tract hemorrhage are important to build a scoring system to assess the prognosis of Acute-on-chronic Liver Failure and may be useful to guide clinical treatment.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers ; blood ; Child ; Child, Preschool ; Chronic Disease ; Female ; Hepatic Encephalopathy ; complications ; Hepatitis, Viral, Human ; complications ; epidemiology ; Humans ; Hyponatremia ; complications ; Infant ; Liver Failure, Acute ; blood ; etiology ; pathology ; Logistic Models ; Male ; Middle Aged ; Prognosis ; Prothrombin Time ; Retrospective Studies ; Risk Assessment ; Risk Factors ; Severity of Illness Index ; Young Adult
9.A novel primary culture and identification method of human retina gliocyte
Shao-fen, LIN ; Yu-xiang, MAO ; Bin, LI ; Ping, ZHANG ; Jian-liang, ZHENG ; Yan, LUO ; Jie, HU ; Shi-bo, TANG
Chinese Journal of Experimental Ophthalmology 2012;30(1):17-19
BackgroundHuman retinal gliocytes play an important role in proliferative diseases,which are the basis of in vitro studies.Researchers have cultured human retinal gliocytes in the past.In our study,we found that the cells we cultured presented a unique shape different from those by other researchers.ObjectiveThis study was to design to produce a new culture and purification method for retinal gliocyte in vitro.Methods Retinal tissue was isolated from human eyeballs and digested using the two-step digestion method (2% pancreatin and 0.133%collagenase Ⅵ) to harvest the retinal glio cytes.The cells were collected and cultured in endothelial cell-targeted nutrient culture containing 10% fetal calf serum and supplemented with β-endothelial cell growth factor (ECGF) and sodium heparin,and the culture dishes were coated with fibronectin(FN) to promote the attachment of retinal gliocyte.During the culturing process,the gliocytes were identified by the observation of morphological characteristic and regular histological examination.The identification of the cells also was performed by immunochemistry targeting glial fibrillary acidic protein (GFAP),Vimentin,neuron specific enolase ( NSE ),S-100,CD34,and Ⅷ factor.Results Retinal gliocytes were isolated successfully from the human retina by the two-step digestion method.Primary cultured cells attached after 72 hours and achieved confluency between day 9 and 10 that were aligned petaliform in shape.Regular histological examination after H&E staining showed blue cell nuclei and light red cytoplasm.The target cells presented with strong responses for GFAP and Vimentin and no response for NSE,S-100,CD34 and Ⅷ factor.ConclusionsLarge amount of purified human retinal gliocytes can be obtained by two-step digestion and cultured in endothelial cells-targeted culture medium supplemented with β-ECGF and sodium heparin in plates coated with FN.The cultured cells expressed markers for retinal gliocytes.However,specific features of these cells remain to be further elucidated.
10.The short-term efficacy of nucleoside analogue on the treatment of acute-on-chronic liver failure.
Xiao-yan LIU ; Hui-fen WANG ; Jin-hua HU ; Wei-ping HE ; Hai-qing WANG ; Na LIU
Chinese Journal of Hepatology 2010;18(11):845-848
OBJECTIVETo discuss the short-term efficacy of nucleoside analogue on the treatment of hepatitis B virus induced acute-on-chronic liver failure (HBV-ACLF).
METHODS348 patients with HBV-ACLF in our hospital from January 2006 to June 2008 were selected. According to the stages of patient's condition and whether or not with nucleoside analogue administration, The patients were divided into early stage therapy group, early stage control group, middle stage therapy group and middle stage control group. Groups were compared on the basis of stages. The clinical data were analyzed using chisquare test and independent-Samples T Test.
RESULTSAfter 2 weeks of therapy no significant difference found between the therapy group and the control group. the total bilirubin (TBil) and alanine transaminase (ALT) showed no significant difference between the middle stage therapy group and the control group in 4 weeks of therapy. However significant differences existed in the HBV DNA negative rate, PTA, the model for end-stage liver disease (MELD) score and the improvement rate between the two groups (P<0.05, P<0.01). Only the 4 week survival rate and HBV DNA negative rate showed significant difference in patients who received anti-virus therapy on the early stage as compared to the control group.
CONCLUSIONAnti-virus therapy with nucleoside analogue is an effective way for the treatment of those patients with HBV-ACLF and can increase the survival rate.
Adult ; Antiviral Agents ; therapeutic use ; End Stage Liver Disease ; drug therapy ; virology ; Female ; Hepatitis B virus ; drug effects ; Hepatitis B, Chronic ; drug therapy ; Humans ; Male ; Middle Aged ; Nucleosides ; therapeutic use ; Treatment Outcome