According to the base complementation, the principle antisense technology is the sequence specific binding of complementary antisense nucleic acids or their chemical modification to the nucleic acids in cells, resulting in the suppression or prevention of gene translation. For the treatment of cancer, a lot of antisense drugs have been designed, which target the genes involved in the pathogenesis of cancer. And some of them have been in clinical trials. The author reviewed the progress of the clinical trials of these antisense drugs.

Aged ; Female ; Foreign Bodies ; pathology ; Humans ; Laryngeal Diseases ; Larynx ; pathology ; Vestibule, Labyrinth

Chemical constituents of the essential oil in above-ground and root of Bupleurum malconense and root of B. chinense were investigated by GC-MS compiled with automated mass spectral deconvolution and identification system (AMDIS) and retention index. The results showd that the components of essential oil in B. malconense have some similarities with the one in B. chinense, and both of them have the higher content of caryophyllene oxide which is an active component of anti-inflammatory and analgesic. These results suggested that as a local substitute, B. malconense has a certain scientific basis of the treatment for cold fever.
Bupleurum ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; therapeutic use ; Fever ; drug therapy ; Mass Spectrometry ; Oils, Volatile ; chemistry ; Plant Roots ; chemistry

Objective To analyze the detection rate of lung cancer during 2009-2013 in Shaoxing of Zhejiang province and its time trend. Methods The data of lung cancer in Shaoxing from 2009 to 2013 with a total coverage population of 21 970 825 person-year (male 10 993 657 person-year, female 10 977 168 person-year), was collected to calculate the detection rate, age adjusted detection rate, percentage change of incidence (PC), etc. The annual percentage change (APC) of incidence was estimated by linear regression based on the logarithm of the incidence rates observed. Results During 2009-2013, a total of 9 973 new cases were diagnosed as lung cancer with the sex ratio of male to female being 2.44∶1. The detection rate of lung cancer was 45.39/100 000 (male 64.35/100 000, female 26.41/100 000), age-standardized detection rate by Chinese standard population (ASR China) was 20.09/100 000 (male 28.37/100 000, female 11.66/100 000), age-standardized detection rate by world standard population (ASR world) was 27.26/100 000 (male 37.83/100 000, female 15.73/100 000). The cumulative rate and truncated rate of those aged 0-74 and 35-64 were 3.40% (male 4.80%, female 1.91%) and 39.90/100 000 (male 54.44/100 000, female 24.81/100 000). The detection rate of lung cancer increased from 34.29/100 000 in 2009 to 59.91/100 000 (APC=13.88%, P=0.006<0.05), among males the detection rate increased from 50.60/100 000 to 82.91/100 000 (APC=12.52%, P=0.011<0.05), and the female detection rate increased from 17.81/100 000 to 36.78/100 000 (APC=17.70%, P=0.005<0.05). In Shaoxing, the detection rate of lung cancer increased obviously among the residents over 45 years of age. For each age group except 15-and 20-, the male detection rate was higher than the female incidence. Conclusion The men who were 45 years and older were at a higher risk of lung cancer in Shaoxing. The detection rate of lung cancer increased year by year from 2009 to 2013 in Shaoxing, especially among women over 65 years of age.

Objective To analyze the detection rate of thyroid cancer from 2009 to 2013 in Shaoxing of Zhejiang province and its time trend.Methods The data of thvroid cancer in Shaoxing from 2009 to 2013 was collected to calculate the detection rate,age adjusted detection rate and percentage change of incidence,and the annual percentage change (APC) of incidence was estimated by linear regression based on the logarithm of the incidence rates observed.Results lDuring 2009-2013,a total of 3 290 new cases were diagnosed as thyroid cancer with the sex ratio of male to female being 1 ∶ 3.69.The detection rate of thyroid cancer was 14.98/100 000 (male 6.39/100 000,female 23.59/100 000),with age-standardized detection rate by Chinese standard population (ASR China)9.13/100 000(male 3.94/100 000,female 14.31/100 000),and age-standardized detection rate by world standard population(ASR worll) 10.39/100 000(male 4.47/100 000,female 16.32/100 000).The detection rate of thyroid cancer increased from 7.26/100 000 in 2009 to 27.26/100 000 (APC =39.65％,P＜0.05),with the detection rate among males increasing from 2.42/100 000 to 12.65/100 000(APC =48.44％,P＜0.05),and the detection rate among female increasing from 12.15/100 000 to 41.96/100 000(APC =37.58c％,P＜0.05).In Shaoxing,most of the new thyroid cancer cases were from 25 to 59 years old,and the highest incidence happened in the group of 50-54 years.For each age group,the detection rate in female was almost over two-folls higher than the incidence in male.Conclusion The detection rate of thyroid cancer was increased year by year from 2009 to 2013 in Shaoxing,especially among the young and middle-aged women.The corresponding measures must be taken to control the risk factors of thyroid cancer.

Objective To analyze the incidence of malignant tumor in Shaoxing of Zhejiang Province in 2012.Methods Descriptive analysis was carried out based on our chronic disease monitoring information management system.Age-standardized incidence was calculated according to Chinese standard population in 1982 and the Segi's world population.Results The crude incidence of malignant tumor was 291.34/100 000,age-standardized rate by Chinese standard population (ASR China) and by world population was 137.29/100 000 or 176.72/100 000,respectively.The cumulative rate of the 0-74 years old group was 20.27％ and the cut rate of the 35-64 years old group was 301.49/100 000.Male crude incidence of cancer and ASR China were 330.46/100 000 and 149.28/100 000,respectively; for female,the figure was 251.93/ 100 000 and 124.79/100 000,respectively.The crude incidence and ASR China were 364.85/100 000 and 171.77/100 000 in urban area residents,and 277.86/100 000 and 130.93/100 000 for those living in rural areas.Age-specific incidence of the 0-29 years old group remained low,although the incidence was increased after 30 years old and reached the peak in the 80-84 years old group.The most common malignancy were lung cancer,gastric cancer,colorectal cancer,liver cancer,breast cancer,thyroid cancer,esophageal cancer,pancreatic cancer,cervical cancer and leukemia (76.77％).Conclusions Lung cancer,gastrointestinal cancer and female reproductive system cancer were most common malignancy in Shaoxing of Zhejiang Province in 2012.The incidence of thyroid cancer,leukemia and brain tumor is significantly increased over the past few years,which may need effective control and prevention strategies.

Objective: To study the antioxidant activity of Clematis chinensis Osbeck polysaccharide(CCP).Methods:① To assay the scavenging activity of CCP on hydroxyl radical and superoxide anion in vitro.② To analyze the effect of CCP on red blood cell autoxidation hemolysis induced by H2O2 by colorimetry.③ To study the effect of CCP on acute hepatic injury of the mice induced by carbon tetrachloride.Results: CCP could eliminate the hydroxyl free radical and the superoxide anion free radical,reduce red blood cell autoxidation hemolysis induced by H2O2,raise the activities of SOD and GSH-Px in hepatic injury mice's serum and liver apparently,reduce the content of MDA and liver index notably.Conclusion: CCP has the significantly action of anti oxidation activity in vitro and in vivo which relates to removing oxygen free radical.

Objective To observe the function changes of right ventricular in chronic obstructive emphysema after lung volume reduction surgery. Methods Twenty-six patients with chronic obstructive emphysema undergoing lung volume reduction surgery were examined by color Doppler echocardiography to determine systolic function and diastolic function of right ventricle in preoperative days and postoperative days of 5～7; 12～14; 1 month and 3 months. Results Contraction of right ventricle: comparing with preoperative parameters, there were significant decrease (P0.05) of those above mentioned in postoperative days 12～14. It recovered at 3 month after operation. Relaxation of right ventricle: the swift filling peak flow-rate in earlier relax period (E) and E flow-rate integra (ETVI) decreased meanwhile swif filling peak flow-rate in late relax period (A) and A flow-rate integra (ATVI) increased in the postoperative days 5～7 (P

Objective To evaluate the apphcation of wireless mobile infusion system in the outpatients and emergency patients.Methods A total of 566 outpatients and emergency patients with infusion were divided into the observation group and the control group according to the order of infusion.The observation group with 297 cases was used wireless mobile infusion system,and the control group with 269 cases was used traditional infusion method.The reception time,infusion waiting time and satisfaction for nurses' job of patients between two groups were compared.The satisfaction for job of nurses among different work models were compared,too.Results The reception time,infusion waiting time of patients in the observation group were less than those in the control group:(2.07±0.26) min vs.(3.17±0.26) min,(9.89±0.31) min vs.(13.49± 0.21) min,and there were significant differences,P＜0.01.The satisfaction for nurses' job of patients in the observation group were better than those in the control group,x2=8.921,7.064,5.251,and there were significant differencs,P＜0.05.The satisfaction for job of nurses in the observation group was better than that in the control group,and there was significant difference,P＜0.05.Conclusion Wireless mobile infusion system can improve work efficiency,guarantee the safety of infusion,improve patients and nurses' satisfaction.

Objective To investigate the effect of tannic acid on glomerular mesangial cells (GMC),and to clarify the mechanism of tannic acid in improving the pathological changes of diabetic nephropathy (DN)from the aspect of oxidative stress and micro-inflammation. Methods The glomerular mesangial cells were treated with glucose (30 mmol·L-1 )or advanced glycosylation end-products (AGEs)bovine serum albumin(BSA)(250 mg·L-1 )and then different concentrations of tannic acid (10,20,40 and 80μmol·L-1 )were added into the GMC.The cells cultured by normal glucose or treated with BSA were used as control groups and then the level of malonic dialdehyde (MDA), glutathione peroxidase (GSH-Px ), superoxide Dismutase (SOD ), CAT (Catalase ) activities and 8-hydroxy-2′-deoxyguanosine(8-OHdG)levels in the culture supernatant 48 h after culture were determined by colorimetry and ELISA method. The expressions of intercellular cell adhesion molecule-1 (ICAM-1 ) protein, monocyte chemotactic protein 1 (MCP-1 ) and ICAM-1 mRNA in GMC were detected by immunohistochemical staining and RT-PCR method.Results Compared with high glucose and AGEs groups,the MDA levels in tannic acid groups were reduced significantly(P<0.05);the activities of GSH-Px,SOD and CAT were increased significantly(P<0.05 or P<0.01);the 8-OHdG levels in annic acid groups were significantly reduced (P<0.05). Compared with high glucose and AGEs groups,the expressions levels of ICAM-1 protein in 40 and 80μmol· L-1 tannic acid groups were decreased (P<0.05 ). The mRNA expressions levels of MCP-1 and ICAM-1 were significantly lower than those in high glucose group (P<0.01 ).Conclusion Tannic acid could protect GMC against the damage of oxidative and inflammatory mediators,thereby delaying and improving the glomerular lesions of DN.