Objective To study the clinical effects of breast-preserving surgery in early-stage breast cancer. Methods From March 2002 to March 2007, 27 cases of early-stage breast cancer were treated with breast-preser-ving surgery. The control group had 28 cases, treated with radical mastectomy. Survival rate and recurrence rate were observed. Results The short-term progress of breast-preserving surgery in early-stage breast cancer had no significant difference with that of radical mastectomy. Plastic effects of breast were satisfied. Conclusion Breast- preserving surgery in early-stage breast cancer had favorable effects both in progress and in plastic. Therefore, it is warranted for wide use.

411 subjects were divided into six groups according to their FPG levels.And OGTT was carried out.Result showed that along with the FPG increasing, HOMA ?-cell index and ?I30 /?G30 were decreased progressively, and the fasting serum proinsulin level and ratio of proinsulin /insulin were increased markedly.The area under insulin curve decreased gradually when FPG was above 8 mmol/L. In conclusion, FPG is a reflection of islet ? cell function.

The microenvironment after peripheral nerve injury becomes complicated for axon regeneration, which can be promotion, inhibition, or both. This paper summarized the researches about the effect of microenvironment on axon regeneration after peripheal nerve injury.

Objective To investigate the resistance-related genes of Shigella sonnei with decreased susceptibility to fluoroquinolones.MethodsA total of 131 strains of Shigella sonnei were analyzed for their antimicrobial susceptibility.Mutations within the quinolone resistance determining regions (QRDR) of gyrA and parC were detected by polymerase chain reaction (PCR) and PCR products were then sequenced. Meanwhile, the plasmid-mediated quinolone resistance (PMQR) genes,qnr and aac(6')-Ib-cr were screened by PCR.ResultsResistance rates of 131 Shigella sonnei isolates to nalidixic acid,tetracycline,ampicillin and trimethoprim-sulfamethoxazole were 100.0％,93.9％,93.2％ and 92.8％,respectively.All strains were susceptible to norfloxacin,ciprofloxacin,levofloxacin,while 94％ nalidixic acid-resistant Shigella sonnei strains showed reduced susceptibilities to fluoroquinolones.All of nalidixic acid-resistant Shigella sonnei strains presented a single mutation at codon 83 (Ser→Leu) of gyrA genes,but no mutations were detected in parC gene.And PMQR genes qnr and aac (6’)-Ib-cr were not detected.Conclusions The nalidixic acid-resistant Shigella sonnei strains with reduced susceptibility to fluoroquinolones are common in the clinical practice,which may mainly due to a single mutation at codon 83 (Ser→Leu) of gyrA genes.

ABSTRACT:Objective To validate that relaxin can resist hepatic fibrosis at the cellular level and explore its molecular mechanism in order to provide experimental basis for the treatment of liver cirrhosis.Methods Cultured HSC-T6s were treated with different concentrations (20,50 and 100 ng/mL)of recombinant human relaxin-2 (RLX-2).The proliferation of HSC-T6 was measured by MTT colorimetric assay.The content of type Ⅰcollagen in the cell culture supernatant of each group was detected by ELISA at 48 h of drug intervention;RT-PCR was used to detect the mRNA expressions of CTGF and TGF-β1 in HSC-T6 at 48 h of drug intervention.Results RLX-2 inhibited the proliferation of HSC and reduced type Ⅰ collagen content of HSC cells.It also inhibited the CTGF mRNA expression of HSC,but did not have a significant effect on the expression of TGF-β1 mRNA. Conclusion In the experiment of culturing HSC-T6 in vitro,RLX-2 may play a role in rat liver fibrosis by inhibiting cell proliferation and type Ⅰ collagen and CTGF mRNA expressions.

Production of bioethanol using starch as raw material has become a very prominent technology. However, phytate in the raw material not only decreases ethanol production efficiency, but also increases phosphorus discharge. In this study, to decrease phytate content in an ethanol fermentationprocess, Saccharomyces cerevisiae was engineered forheterologous expression of phytase on the cell surface. The phy gene encoding phytase gene was fused with the C-terminal-half region of α-agglutinin and then inserted downstream of the secretion signal gene, to produce a yeast surface-display expression vector pMGK-AG-phy, which was then transformed into S. cerevisiae. The recombinant yeast strain, PHY, successfully displayed phytase on the surface of cells producing 6.4 U/g wet cells and its properties were further characterized. The growthrate and ethanol production of the PHY strain were faster than the parent S. cerevisiae strain in the fermentation medium by simultaneous saccharification and fermentation. Moreover, the phytate concentration decreased by 91% in dry vinasse compared to the control. In summary, we constructed recombinant S. cerevisiae strain displaying phytase on the cell surface, which could effectively reduce the content of phytate, improve the utilization value of vinasse and reduce the discharge of phosphorus. The strain reported here represents a useful novel engineering platform for developing an environment-friendly system for bioethanol production from a corn substrate.
6-Phytase ; metabolism ; Biofuels ; Ethanol ; chemistry ; Fermentation ; Industrial Microbiology ; Saccharomyces cerevisiae ; metabolism ; Starch ; chemistry ; Zea mays ; chemistry

Objective To investigate the level of serum retinol-binding protein 4 (RBP4) and related factors in chronic hepatitis C (CHC). Methods Fifty-six patients with CHC (CHC group) and 35 healthy volunteers (control group) were selected. Serum RBP4 level was measured by ELISA method.Fasting blood glucose ( FBG ), triglycerides (TG), total cholesterol ( TC ), alanine aminotransferase (ALT),γ-glutamyl transpeptidase (γ-GT) were measured, HCV-RNA level was tested by qualitative polymerase chain reaction(q-PCR). Results There were no significant difference in FBG, TC,TG, γ-GT between two groups (P ＞ 0.05 ). Serum RBP4 level in CHC group [(33.38 ± 6.43 ) mg/L] was higher than that in control group [(26.11 ± 3.35) mg/L](P＜ 0.01),the CHC patients with ALT normal (26 cases) had significantly higher RBP4 level [( 38.96 ± 4.09) mg/L] compared with ALT abnormal [30 cases, ( 28.53 ± 3.43 ) mg/L](P ＜ 0.01 ). ALT level was negative with RBP4 in CHC group (r = -0.6368, P ＜ 0.05 ). Conclusion Serum RBP4 level is significantly associated with CHC and negatively correlated with ALT level,but not associated with FBG, TC,TG, γ-GT and HCV-RNA.

With the increasing demand for liver transplantation,some previously abandoned donors,called marginal donor,started to be involved in clinical liver transplantation,which raises higher requirement on the organ preservation methods.Normothermic perfusion is regarded to be superior to the currently adopted hypothermic perfusion,and plays an important role in the organ preservation of marginal donors.This review will summarize the clinical applications of liver normothermic perfusion in transplantation and repair,as well as its roles in basic research.

Objective To investigate training of the ultrasound-guided modified Seldinger technique(MST)for cannulation of PICC.Method Twenty-nine nurses trained with the traditional training on ultrasound-guided MST during December 2009 to December 2011 were set as control group and another 29 nurses trained with the systematic training during December 2011 to December 2013 were as experiment group.The training effect were compared between the two groups after 2 months training.Results The theoretical knowledge in the experiment group was improved significantly compared with the control group. Moreover, the success rate of catheterization in the experiment group was 100.0%,significantly higher than that in the control group(both P<0.01).Conclusion Systematic training system is more effective than the traditional training.It can improve the nurses’technical and theoretical knowledge on ultrasound-guided MST for PICC.

Objective To investigate the clinical significance of ultrasound guided biopsy of peritoneum in diagnosis of unex-plained ascites .Methods A total of 95 patients with unexplained ascites after conventional examination were collected ,then under-went ultrasound inspection which showed thickened peritoneum and the relations to surrounding tissues around the planned perito-neum puncture point ,and conducted the peritoneal biopsy .Results A total of 95 were successfully biopsied with 2 -4 peritoneal tissues .Pathological examination showed that 37 cases (38 .9% ) were diagnosed as peritoneal metastatic carcinoma ,41 cases (43 . 1% ) with peritoneal tuberculosis ,5 cases (5 .2% ) with peritoneal mesothelioma ,1 case (1 .1% ) with eosinophilic granuloma ,4 ca-ses (4 .2% ) with fibrous hyperplasia ,and 7 cases (7 .4% ) with chronic inflammatory cell infiltration .The positive rate of ultra-sound guided peritoneal biopsy was 88 .4% .Conclusion The ultrasound guided biopsy of peritoneum is of clinically great signifi-cance in diagnosis of unexplained ascites .