Objective:To explore the effect of zuoguiyin and its partial prescriptions on cellular immune function in senile mice.Methods:Healthy male ICR senile mice were divided into 4 groups at random: old contrast group, ZGY group, prepared rhizome of rehmannia(PROR) group and none prepared rhizome of rehmannia(NPROR) group. The senile mice had been drunk zuoguiyin and its partial prescriptions for 75 days, then observing the differences through thymic index, spleen index, activity of T lymphocyte and the level of IL-2 after using different drugs.Results:Acted by ZGY, ZGY had enhanced cellular immune function of senile mice, appearing that thymic index, spleen index, activity of T lymphocyte and the level of IL-2 were distinctly higher than those of the old contrast group. Compared to ZGY,prepared rhizome of rehmannia also enhanced the activity of T lymphocyte. However, it had no effect on thymic index, spleen index and the level of IL-2.So its effect on anti-senility was not better than ZGY. None prepared rhizome of rehmannia group had no distinct effect on those indexes.Conclusion:Zuoguiyin had an action of antisenility by improving cellular immune function.

BACKGROUND: The method of replenishing kidney has becoming one of the therapies for anti-senility in gerontology in Chinese medicine.OBJECTIVE: To probe into the effects of the formula for nourishing kidney yin-zuoguiyin on serum malondialdehyde (MAD) content and superoxide dismutase (SOD) activity in metabolism of free radical.DESIGN: Complete randomized control experiment.SETTINGS: Microbiology and Immunology Department of Guangdong Medical College.PARTICIPANTS: The experiment was performed in Immunology Research Room of Wannan Medical College from September 2002 to July 2004. Forty male senile ICR mice were employed, provided by Institute of Experimental Animal of Medical Scientific Academy in Sichuan. They were randomized into senile control, zuoguiyin group, single shudi (Radix Rehmanniae Praeparata) group (single group) and shudi (Radix Rehmanniae Praeparata) removal group (removal group), 10 mice in each one. Zuoguiyin composes of shudi (Radix Rehmanniae Praeparata), shamyao (Rhizoma Dioscoreae), gouqi (Fructus Lycii), zhi gancao (Radix Glycyrrhizae Praeparata),fuling (Poria) and shangyurou (Fructus Corni) at the ratio of 9:6:6:3:4:5. After decocted routinely, the solution in zuoguiyin group was concentrated to 0.577 5 g/mL of raw herbs contained; that in single group was to 0.157 5 g/mL and that in removal group was to 0.42 g/mL. The mixed medical solutions of 100 g/L were prepared successively in each group.INTERVENTIONS: In zuoguiyin group, single group and removal group,solutions of zuoguiyin and shudi (Radix Rehmanniae Praeparata) and solution with shudi (Radix Rehmanniae Praeparata) removed were administrated successively, 4 times/day, 1.5 mL/time, totally for 75 days. No any drink was provided during the medication. The mice in senile control drank pipe water. After the eyeball extracted, blood of 2 mL collected and serum preserved, the thiobarbituric acid method was used to determine MAD content, the xanthine oxidase method was to determine SOD activity and the colorimetric method was to determine general capacity of anti-oxidation in serum.MAIN OUTCOME MEASURES: ① serum MAD content; ② serum SOD activity; ③ general capacity of anti-oxidation in serum.RESULTS: One mouse was dead in senile control and 3 mice in single group. Terminally, 36 mice entered result analysis. ① MAD content:Serum MAD content in zuoguiyin group and single group were lower than that in senile control [(5.280±1.204) μmol/L and (5.886 2.717) μmol/L,(9.533 3.494) μmol/L, (q=3.641-4.657, P ＜ 0.05)]. Serum MAD content in zuoguiyin group and single group were basically same. ② SOD activity: SOD activity in zuoguiyin group and removal group were higher than senile group [(7.008±0.782) mkat/L and (6.989±0.809) mkat/L, (5.908±0.392) mkat/L,(q=3.641-4.657, P ＜0.05)]. SOD activity in zuoguiyin group and removal group were basically same and that in single group and senile control were basically same. ③ General anti-oxidation capacity: The general capacity of anti-oxidation in serum in zuoguiyin group and single group were stronger than that in senile control, that in removal group was weaker than senile control, but the differences were not significant (P ＞ 0.05).CONCLUSION: Zuoguiyin remarkably reduces MAD content and improves SOD activity in serum of senile mice. It is indicated that zuoguiyin acts remarkably on improving metabolism of free radical in senile body,strengthening SOD activity and reducing organic injuries caused by MAD accumulation.

Objective To observe the efficacy of the combination of moxibustion and pressing beans on auricular in the treatment of elderly patients with chronic diarrhea.Methods 45 elderly patients met the diagnostic criteria for diarrhea were divided into two groups,the treatment group (23 cases) received the treatment of combined moxibustion with ear pressure beans,the control group (22 cases) received traditional Chinese medicine treatment.The efficacy was observed and compared after two weeks.Results The effective rate reached 95.6％ in the treatment group,81.8％ in the control group (statistically significant difference,odds ratio 1.16).Conclusion Combined ear pressure beans with moxibustion treatment will receive a significant effect for the elderly patients with chronic diarrhea.The therapy is worth clinical application.

ConclusionBushen-Yitalpowder adjuvant to progesterone therapy can improve the clinical outcome in patients with early threatened abortion.

Objective To investigate the value of modified NIHSS and MRI-PC-ASPECTS for the long-term prognosis in posterior circulation ischemic stroke. Methods Seventy eight patients with posterior circulation ischemic stroke were divided into two groups depended on MRS: good outcome group and bad outcome group. General information of patients was collected and vascular risk factors, NIHSS, MRI-PC-ASPECTS of the two groups were compared. Results The scores of admission NIHSS and highest NIHSS of bad outcome group were higher than that of good outcome group. The scores of MRI-PC-ASPECTS, T admission NIHSS (42-admission NIHSS) and T highest NIHSS of bad outcome group were lower than that of good outcome group (P<0.05). The areas under the ROC curves of MRI-PC-ASPECTS+T highest NIHSS, MRI-PC-ASPECTS, T highest NIHSS and T admission NIHSS were 0.907, 0.821, 0.870, 0.744, respectively (P<0.05). Conclusion NIHSS and MRI-PC-ASPECTS can predict the functional prognosis in patients with posterior circulation ischemic stroke.

Objective To investigate the changing patterns of Staphylococcus aureus (SA) and its resistance from a paediatric clinic in Beijing Children's Hospital Affiliated to Capital University of Medical Sciences.Methods SA isolated from hospitalized patients in Beijing Children's Hospital Affiliated to Capital University of Medical Sciences from Jan.2003 to Dec.2012 were analyzed.Antimierobial susceptibility was determined by disk diffusion method and Phoenix 100 microbiological system.Results were analyzed according to the 2012 guidelines of Clinical and Laboratory Standard Institute.WHONET 5.6 software was used to analyze the data.Results The clinical isolates of SA from inpatient were 2 843.The positive rates of SA were 10.0％,8.7％,9.0％,11.6％,12.7％,11.7％,10.5％,10.8％,11.9％ and 11.7％ form 2003 to 2012,respectively.The positive rate of Methicillin-resistant Staphylococcus aureus (MRSA) from inpatients was 10.9％.The positive rates of MRSA were 3.1％,5.4％,6.4％,4.1％,6.0％,10.6％,11.2％,12.7％,14.1％ aid 20.1％ form 2003 to 2012,respectively.The resistant rate of Methicillin-susceptible Staphylococcus aueus and MRSA to Penicillin were 91.4％ and 100.0％,Oxacillin were 0 and 100.0％,Cefuroxime were 0.5％ and 87.7％,Ceftriaxone were 0.1％ and 90.3％,Amoxicillin-clavulanic acid were 0.9％ and 86.7％,Erythromycin were 72.4％ and 92.2％,Clindamycin were 50.6％ and 80.3％,Ciprofloxacin were 2.2％ and 30.1％,Trimethoprim-sulfamthhoxazol were 12.2％ and 13.9％.Vancomycin-intermediate and Vancormycin-resistant Staphylococcus aureus were not found.Conclusions The prevalence of MRSA is increasing in paediatric inpatients.And MRSA isolates exhibit multidrug resistance.The sequential surveillance about SA is very important for guiding rational antimicrobial therapy and effective control of infections in paediatric patients.

Objective The current study was to investigate the clinical and pathological features of macroscopic hematuria associated acute kideny injury (MH-AKI) in patients with IgA nephropathy (IgAN) and its associated prognostic factors.Methods We performed an analysis on the clinical and pathological features of biopsy-proven IgAN patients diagnosed during 2004 to August of 2007,who had experienced MH associated AKI.The patients enrolled in the study were followed up.The renal lesions were evaluated with Katafuchi pathological scores of IgAN.Results 5 patients with IgAN had experienced at least one episode of MH-AKI,accounting for 1.3% of IgAN diagnosed during the same period and 13.5% of IgAN patients with macroscopic hematuria.High proportion of tubules filled with red blood cell casts and tubular injury were demonstrated in all biopsy specimens.Renal function of 3 patients completely recovered within 14 days.One patient (patient 4) got quick recovery in the first month of the disease but completely recovered 418 days later.The other patients only had an incomplete recovery during the 20-month follow-up.The incompletely recovered one had relatively an older age,longer persistence of MH,more sclerotic glomeruli and a more severe tubule-interstitial damage.Conclusion AKI is commonly seen during episodes of MH in patients with IgAN.Not all patients with MH-AKI showed a complete recovery.Age,duration of MH,proportion of glomerular sclerosis and the severity of tubular necrosis might be the risk factors of an incomplete recovery of renal function.

0.05). Conclusion: The PGI 2 level in dental pulp tissue is closely related to the degree of pulp pain.

Objective: To study the microstructure of e-polytetra fluoroethylene (e-PTFE) membrane and chitin membrane and to observe the growth of osteoblasts on the membrane. Methods: Light microscope and scanning electronic microscope were used to study the microstructure of e-PTFE membrane and chitin membrane. Cell culture methods and cell counting were used to observe the growth of osteoblasts on the two kinds of membrane. Results: The chitin membrane showed a compact structure without any pore in it. There were many shallow dents with the diameter of 5-8 ?m on it's surface. However, in e-PTFE membrane many tiny lined cracks (1-5 ?m in width and 5-15 ?m in length) in elliptic structure were observed. The adhesion and proliferation of osteoblasts on the two kinds of membranes were both well. ALP measurement showed that there were no significant difference between each of the two membrane groups and the control. Conclusions: Both membranes have no negative effects on the growth of osteoblasts. Both membranes are biocompatibile and their microstructure is appropriate for guided bone regeneration materials.

AIM: To dissociate and determine the chemical components of volatile oil from Salsola col-lina Pall.in Shandong Province METHODS: The chemical components of volatile oil were analyzed by gaschromatography-massspectraphy(GC/MS). RESULTS: Fifty-nine constituents were identified,which accounted for 87.63% of volatile oil,the major chemical componets in the volatile oil were terpenes and terpene ramifications. CONCLUSION: This method is reliable,stable and repeatable.the data can provide resources for expolitation of Salsola col-lina Pall.