Objective To investigate the advantage and diagnosis rate of different image examination in breast diseases.Methods From January 2010 to February 2014,all image examination data of 389 breast disease pa-tients who received surgical operation were collected and analyzed retrospectively.All patients underwent color Doppler ultrasound examination.Breast X-ray photographic examination was performed in 325 patients,and MRI ex-amination in 188 patients,while 122 patients received MSCT examination.Results All surgical specimens were ex-amined and diagnosed pathologically.Of all the patients,176 patients were diagnosed with breast cancer(including 117 cases of infiltrating ductal carcinoma,48 cases of mucinous carcinoma,and 11 cases of carcinoma simplex),213 patients were confirmed as benign lesions (including 86 cases of adenomas,35 cases of intraductal papilloma,14 cases of lipoma,26 cases of atypical hyperplasia,34 cases of cystic hyperplasia,3 cases of fibroids,12 cases of adenomyosis, 2 cases of phyllodes tumor,and 1 cases with inflammation).The diagnosis rate with color Doppler ultrasound was 91.0%(354 /389),and the results showed suggestive of malignancy was 75.0%(132 /176 cases).The diagnosis rate with breast X-ray photographic examination was 89.5% (291 /325 cases),and suggestive of malignancy rate was 89.8%(158 /176).The diagnosis rate with MRI was 92.0%(173 /188),suggestive of malignancy was 93.8%(165 /176).The diagnosis rate with MSCT was 91.8% (112 /122),while the suggestive of malignancy was 92.0%(162 /176).Conclusions Both MRI and MSCT have a high diagnostic value than other image examination in discriminat-ing benign and malignant breast diseases.However,there is no difference in diagnosis rate in breast diseases whenever color Doppler ultrasound,breast X-ray photographic examination,MRI or MSCT was used.

Objective:To explore the effect of zuoguiyin and its partial prescriptions on cellular immune function in senile mice.Methods:Healthy male ICR senile mice were divided into 4 groups at random: old contrast group, ZGY group, prepared rhizome of rehmannia(PROR) group and none prepared rhizome of rehmannia(NPROR) group. The senile mice had been drunk zuoguiyin and its partial prescriptions for 75 days, then observing the differences through thymic index, spleen index, activity of T lymphocyte and the level of IL-2 after using different drugs.Results:Acted by ZGY, ZGY had enhanced cellular immune function of senile mice, appearing that thymic index, spleen index, activity of T lymphocyte and the level of IL-2 were distinctly higher than those of the old contrast group. Compared to ZGY,prepared rhizome of rehmannia also enhanced the activity of T lymphocyte. However, it had no effect on thymic index, spleen index and the level of IL-2.So its effect on anti-senility was not better than ZGY. None prepared rhizome of rehmannia group had no distinct effect on those indexes.Conclusion:Zuoguiyin had an action of antisenility by improving cellular immune function.

Objective To discuss the feasibility and safety of early enteral nutrition (EN) on treatment of severe acute pancreatitis (SAP) and its influence. Methods The advancement about application of early EN on treatment of SAP in recent years were reviewed. Results In patients with SAP, early EN via catheter placed in the jejunum could protect the integrity of intestinal barrier and reduce infectious complications. But no consensus had been reached about the starting time, ingredient and infusion mode yet. Conclusion Early EN may have positive effects on treatment of SAP, but further researches are still needed.

Objective To detect normal rat jejunum epithelial cells to learn about the distribution of peptide YY(PYY)receptor and measured two important parameters of PYY receptor so as to explore the target that PYY binds to and display its physiological function.Methods Receptor radioligand assay was conducted by using 125I-PYY to detect normal rat jejunum epithelial cells.We also measured receptor dissociation constant(Kd),the affinity or the ability that the receptor could bind ligand;maximum binding capacity(Bmax),the concentration of binding site;and Hill coefficient.Results There were PYY receptors in normal rat jejunum epithelial cells.Dissociation constant(Kd)of PYY receptor was(386.69?129.95)pmol/L.Maximum binding capacity(Bmax)was(303.21?116.85)fmol/mg protein.Hill coefficient was 1.PYY receptor had high affinity.Conclusion There are distributions of PYY receptors in normal rat jejunum epithelial cells.Kd means that PYY receptor has high affinity.Bmax means that the number of receptors is limited and decides the characters of saturability.We think that PYY directly inhibits secretion by binding to PYY receptor in jejunum epithelial cells when PYY inhibiting jejunum secretion.

Objective To evaluate the dynamic MR imaging findings of peripheral cholangiocarcinoma (PCC) of the liver. Methods Seventeen patients with PCC underwent dynamic MRI during the early, middle, and delayed phase after contrast medium administration. Enhancement pattern of the tumor, the wedge-like enhancement area peripheral to the tumor, dilatation of the intrahepatic biliary ducts, vascular involvement, and extrahepatic tumor invasion were evaluated. Results Early peripheral enhancement with progressive and concentric filling by contrast medium was the most frequent enhancement pattern (9 patients). A wedge-like enhancement area was observed in 10 patients. Ductal dilatation was found in 11 patients. Vascular involvement and extrahepatic invasion were seen in 8 and 2 patients, respectively. Conclusion Enhancement pattern is relative to the character of pathology (quantity and distribution of cell and fibrosis). Early peripheral enhancement with progressive and concentric filling by contrast medium may be specific for PCC.

The effects of monoterpene perilly alcohol (POH) alone or in combination with STI571 on the proliferation and apoptosis of the cell line K562 positive for Bcr/Abl were investigated. By using cell culture, the effect of the drugs on the proliferation of the cells was studied. TUNEL and flow cytometry assay of FITC-Annexin V and PI labeled cells were applied to detect the effects of the drugs on the apoptosis of the cells. The results showed that at 36 h, IC50 of POH on K562 positive for Bcr/Abl and HL-60 negative for Bcr/Abl were 81.0 +/- 11.3 micromol/L and 113.6 +/- 23.4 micromol/L respectively (P>0.05). POH could inhibit the proliferation of K562 in a time- and dose-dependent manner with the inhibitory rate of 100 micromol/L POH on K562 cells at 36 h being (53.2 +/- 3.65)%. K562 cells were more sensitive to STI571 than POH. IC50 of STI571 on K562 cells in 36 h was (0.256 +/- 0.054) micromol/L. In a time- and dose-dependent manner, POH induced the apoptosis of K562 cells with the percentage of apoptotic cells by 100 micromol/L POH at 40 h being (21.0 +/- 3.3)%. Both 100 micromo/L POH and 0.2 micromol/L STI571 had the same inhibitory effects on the K562 cells at 36 h. But at 12 and 24 h, the inhibitory rate of POH was significantly higher than that of STI571 (P<0.05) and the ability of STI571 inducing apoptosis at 36 h was greater than that of POH. 50 micromol/L, 100 micromol/L and 200 micromol/L POH in combination with 0.2 micromol/L STI571 could obviously increase the inhibitory effects on the cellular proliferation. Combined use of 50 micromol/L, 100 micromol/L, 200 micromol/L with 0.2 micromol/L STI571 could strongly induced apoptosis, especially 200 micromol/L POH in combination with 0.2 micromol/L STI571. It was concluded that the antileukemia effect of POH had no obvious Bcr/Abl positive selectivity. POH can inhibit the proliferation of K562 and induce the apoptosis in a time- and dose-dependent manner. K562 cells were more sensitive to STI571 than POH. POH in combination with STI571 could obviously enhance the abilities of STI571 inhibiting the proliferation and inducing apoptosis of K562 cells.
Antineoplastic Agents/*pharmacology ; Apoptosis/*drug effects ; Dose-Response Relationship, Drug ; Drug Synergism ; Fusion Proteins, bcr-abl/analysis ; HL-60 Cells ; K562 Cells ; Monoterpenes/*pharmacology ; Piperazines/pharmacology ; Pyrimidines/*pharmacology

The source, super-antioxidant activity, biological safety and application in medicine, health food and animal feed industry of natural astaxanthin are reviewed in present paper. The industrialization prospect of astaxanthin in China is also proposed.

OBJECTIVE To introduce process management to procedure and standardization of infection management in common ward.METHODS Establishing and applying hospital infection control process,to use it in internal medicine and surgery wards(eight wards).Retrospective investigation was conducted to analyze the data.RESULTS Before and after using infection control process,there were significant differences between incidence of hospital infection in inpatients(P

This paper expatiates and analyzes the various problems in the course of using and maintaining the medical equipment in hospital.It also puts forward some substantive countermeasures to solve the problems such as training for operators,setting of management regulations,broadening the position and team construction of qualified personnel in medical engineering,etc.

AIM:To observe the effect of lidocaine to the activation of NF-?B and apoptosis in human neutrophils(PMN)in vitro.METHODS:Human PMN were divided into five groups:control group(0.9% sodium chloride);TNF-?(200 ng/L);TNF-?+lidocaine(1.0 mmol/L);TNF-?+lidocaine(2.0 mmol/L),and TNF-?+ lidocaine(4.0 mmol/L).The expression of NF-?B subunit p65 mRNA in the nuclear and I-?B mRNA in the cytosol extracts were analyzed by Western blotting,after co-incubation for 3 h and the content of p65 protein were observed by RT-PCR.PMN apoptosis was detected on flow cytometry after co-incubation 12 h and 24 h.RESULTS:The expression of NF-?B mRNA was significantly decreased and the expression of I-?B mRNA was markedly increased in lidocaine groups(P