Adult ; Ear Canal ; pathology ; Ear Neoplasms ; Female ; Humans ; Neurofibromatoses

Aim To study the protective effect of total extract of astragalus(TEA) on primary rat hepatocytes injured by CCl4 or H2O2 and its mechanism.Methods The primary hepatocytes were isolated by Ⅳ collaganase and injured by CCl4 or H2O2.The contents of malondialdehyde(MDA) and glutathion(GSH),glutathion peroxidase(GSHpx) activity of hepatocytes and the AST and /or ALT level in cultural supernatant were determined by general methods. Results (1) The elevation of MDA content of hepatocytes and AST level in supernatant of cultural hepatocytes,and the loss of GSH content and GSHpx activity induced by CCl4 were restored remarkably by TEA(5～80 mg?L-1);(2)The elevation of ALT level in the supernatant of hepatocytes and MDA content of hepatocytes, and the loss of GSH content and GSHpx activity induced by H2O2 were improved significantly by TEA(5～80 mg?L-1) treatment.Conclusion The results suggest that TEA possess direct protective action on primary hepatocyte in vitro injured by CCl4 or H2O2. These might be associated with its anti-oxidative activity.

Objective To explore the blood level lead and the related high-risk factors in Uigur and Han children in Urumqi by answer tree. Methods Cluster sampling was used to investigate blood lead level of 988 Uigur and Han children aged 6-10 years,the related high-risk factors were investigated by using a standardized questionnaire,the related high-risk of blood lead level was analyzed by answer tree. Results The geometric mean of blood lead concentration of children was 54.46 ?g/L,in 23.28% of the children,the blood lead concentration was more than 100 ?g/L . No significant blood lead level difference was seen between Uigur and Han children. Such high-risk factors as parents occupation,bad habits and housing condition were associated with the blood lead level. Conclusion The blood lead level and lead poisoning rate of children in Urumqi are higher,many factors may influence the blood lead level of children in Urumqi.

Objective To clinically analyze the feature of paroxysmal hemicrania in order to improve our cognition toward it.Methods Eight patients,3 men,5 women,aging 17 to 74 years old,were prospectively analyzed over the past 2 years in our hospital.Results Their age of onset was from 9 to 60years old(mean 42.5±16.3).Seven of the 8 cases were treated with indomethacin,out of whom 5 got an immediate and complete response and one of them remitted partially.Another stopped taking indomethacin because of gastroenteric side effects.She was treated with verapamil and prednisone and partial relief was gained.Conclusions Paroxysmal hemicranial is a rare benign disorder.which needs our improved understanding.The patient who is diagnosed with paroxysmal hemicranial should firsfly receive indomethacin.and standard anti-cluster headache medications or other non-steroid anti-inflammatory drugs is used if she/he can not get relief and (or)tolerate the adverse effects.

ObjectiveThe present study aimed to determine whether or not dual paroxysm proliferator-activated receptor (PPAR) agonist,WY14643,improved the dysfunctioned vascular endothelium in hypertension by reducing endothelium-derived contracting factors ( EDCFs ),and to explore the molecular mechanism it was involved in.MethodsIsometric tension in isolated thoracic aortic rings of spontaneously hypertensive rats was recorded.Endothelium-dependent contractions evoked by acetylcholine in the presence of L NAME were reduced by fenofibrate.Cyclooxygenase 1 ( COX1 ) activities were determined by analyzing the peroxidase activity of cyclooxygenase colorimetrically by using ELISA kit.ResultsCompared to the control group,WY14643 significantly decreased the vasoconstriction in aorta of the SHR rats(P=0.014).PPARα antagonist MK866 enhanced the vascular contractility of SHR rats that were incubated with 10.0μmol/L WY14643( P=0.021 ).PPARΥ antagonist GW9662 did not significantly affect the vascular contractility of SHR rats that were incubated with 10.0 μmol/L WY14643( P=0.061 ).The levels of serum PGFlα(P=0.012),2α( P =0.019) and TXB2(P=0.023) in SHR rats incubated with 10.0 μmol/L WY14643 were significantly lower than the control group,respectively.Under the condition of the existence of vascular endothelium,the expression of COX-1 in SHR rats incubated with WY14643 was significantly lower than that in SHR rats incubated without WY14643 (P=0.017).ConclusionsThose data showed that WY14643 reduced the release of EDCFs,it suggests that WY14643 protects against vascular diseases through the PPAR activators in spontaneous hypertension.

Objective To evaluate the effectiveness of multiple quantitative fluorescence PCR ( QF-PCR) as a rapid technique for prenatal diagnosis of common chromosome aneuploidies , in order to optimize the prenatal diagnosis and shorten the period of diagnosis.Methods Totally 731 amniotic fluid samples of pregnant subjects ,who were referred to the Women′s Hospital School of Medicine Zhejiang University during August 2013 and September 2015, were analyzed with conventional karyotype and the QF-PCR technique by short tandem repeat(STR) markers to detect chromosomes 13,18,21,X and Y aneuploidies.There were 558 samples detected by single blind method , 173 samples detected by double blind method.Results All of the 731 amniotic fluid samples were tested in this study by QF-PCR and the results were compared to the conventional cytogenetic analysis results of the same sample.Totally 558 samples with single blind method detected 5 trisomy 21, 2 trisomy 18, 1 trisomy 13, 1(45,X), 1(47,XXY), 1(47,XYY), 1(47,XXX) and 1(69,XXX), 173 samples with double blind method detected 1 trisomy 21 and 1 trisomy 18.The rapid QF-PCR assay was successful to detect all aneuploidies involving chromosomes 21, 18, 13, X and Y in prenatal diagnosis , which were verified by chromosome karyotype analysis.The results of QF-PCR method were compared with the results of chromosome karyotype analysis , the positive rate was 15/16, the negative rate was 100%(715/715).Non chimeric chromosome abnormality detection rate was 15/15.Conclusions The multiple QF-PCR was a reliable method of detecting common chromosome aneuploidies for rapid prenatal diagnosis.As an important supplement of karyotype analysis , it was of great significance to optimize and improve the prenatal diagnosis system , and might provide more appropriate diagnostic methods for pregnant women.

Objective: To explore the molecular biological mechanism of acupuncture in intervening visual deprivation.
Methods: Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 acupuncture groups (group C1: acupuncture at the unaffected side in early stage; group C2: acupuncture at the affected side in early stage; group D1: acupuncture at the unaffected side in mid-stage; group D2: acupuncture at the affected side in mid-stage; group E1: acupuncture at the unaffected side in late stage; group E2: acupuncture at the affected side in late stage) by the random number table, 6 rats in each group. Rats in the normal group didn’t receive any interventions. The rat model of deprivation amblyopia was established by unilateral eyelid suture in the model group and each acupuncture group. After successful modeling, rats in model group didn’t receive any treatments; rats in the acupuncture groups received acupuncture intervention which began respectively on the 3rd, 12th and 21st day after modeling. Pattern visual evoked potential (P-VEP) and N-methy D-aspartatreceptor-1 (NMDAR1) mRNA expression in visual cortex area 17 were detected at the end of acupuncture intervention in each group.
Results: After the intervention, the P-VEP waveform was significantly changed, with a significantly delayed P100 value (P<0.01) and significantly decreased amplitude of N45-P100 in the model group versus the normal group (P<0.01); the P-VEP waveform was significantly improved, with obviously earlier P100 (P<0.01) and increased amplitude of N45-P100 (P<0.05) in each acupuncture group versus the model group. The improvement effect of acupuncture on the P-VEP waveform in group C1 and C2 was more significant than that in group D1, D2, E1 and E2. The expression of NMDAR1 mRNA of the rat visual cortex area 17 in the model group was significantly lower than that in the normal group (P<0.01); and the expression of NMDAR1 mRNA in the visual cortex area 17 of each acupuncture group was significantly higher than that in the model group (P<0.05); the effect of acupuncture on NMDAR1 mRNA expression in group C1 and C2 was more significant than that in group D1, D2, E1 and E2; and the effect of acupuncture on NMDAR1 mRNA expression was better in group C2 than in group C1 (P<0.05); there was no significant difference in the expression of NMDAR1 mRNA between group D1 and D2, neither between E1 and E2 (P>0.05).
Conclusion: P-VEP waveform is abnormal and NMDAR1 mRNA expression in visual cortex area 17 is decreased in rats with monocularly-deprived amblyopia. Acupuncture in the sensitive period can significantly regulate the abnormal P-VEP waveform and the down-regulate the NMDAR1 mRNA expression of the visual cortex of rats with monocularly-deprived amblyopia. Early treatment in the sensitive period should be the key to obtaining the curative effect.

Digestive diseases, are various types of frequently encounted clinical disorders .at present, diagnosis and differential diagnosis of digestive diseases , in addition to laboratory tests (biochemistry, immunology, cytology and bacterial genetics , etc.), to a large extent still rely on imaging studies.Flow cytometry is a function of the level of single cells or other biological particles for quantitative analysis and sorting means of detection , which can be tens of thousands of high -speed analysis of cells and can simultaneously from a cell a plurality of measured parameters , high speed, high precision, accuracy, etc., become the most advanced cellular quantitative analysis techniques .In recent years, flow cytometry is widely used in the diagnosis and treatment of gastrointestinal cancer , liver cirrhosis, Crohn′s disease, ulcerative colitis and other digestive diseases.

Objective To investigate the change of Th22 cells in peripheral blood of patients with Primary sjogren’s syndrome (pSS)and evaluate clinical significance.Methods 37 patients with pSS from January 2014 to November 2015 were enrolled the study as the observation group.Then 37 healthy adults receiving check-up during the same period were selected as con-trol groups in accordance with the proportion of 1∶1.Flow cytometry was performed to evaluate the levels of Th22 cells in peripheral blood,and tenzyme-linked immuno sorbent assay (ELISA)was used to measure the serum IL-22 levels.Pearson analysis was performed to investigate the relationship between Th22 level and serum IL-22 level,C3,C4,anti-SSA,anti-SSB, ANA antibody,EULAR Sjogren's syndrome disease activity index (ESSDAI)score in observation group.Then the levels of Th22 cells and serum IL-22 were compared among different labial gland pathologic stage in patients with pSS.Results The levels of Th22 cells and serum IL-22 in the observation group were (2.53±1.56)%,718.6±176.8 pg/ml respectively,and significantly higher than (1.24±0.51)%,258.9±72.4 pg/ml in control group (P<0.05).Th22 cell level was positively related with the level of serum IL-22,anti-SSA,anti-SSB and ESSDAI score,and negatively related with the content of C3 and C4 (P<0.05).There were no significant differences in the levels of Th22 cells and serum IL-22 different labial gland pathologic stage in patients with pSS (P>0.05).Conclusion The levels of Th22 cells and serum IL-22 significantly in-creased in patients with pSS,and related to other inflammatory indexes and disease activity,so they may participate in the genesis and development of pSS.

Objective To set up an apoptosis model of prostate cancer cell line and to clone and study the apoptosis related genes. Methods An apoptosis model of prostate cancer cell line DU-145 has been set up through induction by all transretinoic acid (ATRA).During the process of cell apoptosis the apoptosis-related gene was cloned by means of improved PCR-based subtractive hybridization from the apoptosis prostate cancer cell line DU-145 prostate cancer cells. Results During the process of DU-145 cell apoptosis,c-erb B-2 expression,TNF genes and some unknown apoptosis-related gene were observed.This has been accepted by Genebank,the accession number being AF174394. Conclusions ATRA-induced apoptosis of DU-145 cells is a complex process with multiple genes involved,some of which being unknown yet.