BACKGROUND:Deep venous thrombosis is one of the common and fatal complications of total joint arthroplasty.For our clinical practice,we require duplex ultrasound and venography to evaluate the presence of deep venous thrombosis,but the diagnostic tools have several disadvantages.OBJECTIVE:To explore the appropriate and non-invasive biomarkers in the early diagnosis of deep venous thrombosis.METHODS:With the key words of Thrombosis,DVT,Biomarker,Total Joint Arthroplasty,D-dimer,Factor VIII,Thrombin Generation,Fibrin Monomer,P-selectin,Inflammatory Cytokines,Microparticles,Leukocyte Count,Genetic factors,MicroRNAs,a computer-based search was performed for articles published in PubMed database from January 1995 to March 2015.After the initial screening,the reserved articles were further detailed,summarized and concluded.RESULTS AND CONCLUSION: Totaly 66 articles were colected.This brief review is used to analyze the mechanism of the biomarkers which are classified into three levels as folow: detection of blood coagulation,inflammation markers and molecular biology and genetics-based biomarkers.The review also showed a great need of economic and safer biomarkers for the clinic and provided the theoretical basis to diagnose and predict deep venous thrombosis in an early stage.

Professor CHAO En-xiang accumulates abundant clinical experience,thinks highly of syndrome differentiation and treatment in clinic,emphasizes the pathogeny and method of treatment in accordance with formula and Chinese medicine,notices avoiding to be limited by individual experience.He cautiously grasps pathogenesis in clinical diagnosis and treatment,and is good at applying the theories of TCM,emphasizes the application of the theory of the lung and the large intestine being interior-exteriorly related in treating lung system diseases.He is expert in treating acute diseases with purgative therapy. He learns from ancient physicians,but not limited by their academic thinking,and has formed the special thinking on syndrome differentiation and treatment.

Objective To investigate the relationship among the plasma ET,CGRP and chronic atroph-ic gastritis (CAG). Methods One hundred and eighteen cases with GAG, 30 cases with chronic superficial gastritis( CSG) ;30 cases healthy individuals served as control group. The levels of plasma ET,CGRP were measured by radioimmunoassay. Results (1) ET levels were significantly higher in GAG than in CSG and control (P 0. 05) . (2) The levels of plasma ET is statistically different among mild, moderate and severe CAG(P 0.05). (3)The levels of plasma ET were negatively correlated with the levels of plasma CGRP (r = -0. 214,P =0.02). Conclusion It is suggested that ET and CGRP were possibly involved in the pathogenesis of CAG, and their variations in concentrations were related with the extent of CAG.

Objective:To investigate the expression of latent membrane protein-1(LMP-1),NET-1,and vascular endothelial growth factor(VEGF)in non-keratin nasopharyngeal carcinoma(NK-NPC)and its clinical significance.Methods :Sixty biopsy specimens from pathologically-confirmed NK-NPC patients,who were treated in the Affilitated Hospital of Nantong University from May 1999 to May 2003,were included in the present study.Using immunohistochemical techniques (Envison two-step),we examined the expression of LMP-1,NET-1 and VEGF protein in the specimens.The relationship between their expression and elnicopathological parameters and the prognosis was anayzed.Ten specimens of chronic nasopharyngitis served as control.Results:(1)The positive rates of LMP-1,NET-1 and VEGF in NK-NPC were significantly higher than those in the chronic nasopharyngitis(P0.05).(3)NET-1 and VEGF expression in NK-NPC specimens were positively correlated with lymph node metastasis and distant metastasis(P

Objective To evaluate the clinical efficacy and safety for the CAG regimen combined with thalidomide in elderly patients with acute myelogenous leukemia (AML).Methods 51 untreated elderly cases with AML were randomly divided into treatment group (treated with CAG regimen combined with thalidomide,26 cases) and control group (treated with CAG regimen alone,25 cases),and the clinical efficacy and safety were observed.Results After 2 cycles of treatment,the complete remission (CR) rates of treatment group and control group were 61.5 ％ (16/26) and 48.0 ％ (12/25) respectively,the difference was not significant (P =0.404).With regard to prostecdtive efficacy,the difference was markedly significant,1-year CR rates of treatment group and control group were 53.8 ％(14/26) and 24.0 ％ (6/25) respectively (P =0.045),and 2-year CR rates were 42.3 ％ (11/26) and 12.0 ％ (3/25) respectively (P =0.027).During the follow-up of 2 years,overall 1-and 2-year overall survival rates were 73.1％ and 46.1％ in treatment group versus 52.0 ％ and 24.0 ％ in control group.During the follow-up of 2 years,the estimated mean overall survival time in treatment group was 22 months and it was 13 months in control group (P =0.018).The induction mortality rates and myelosuppression showed no difference between the two groups.Compared with control group,the side effect rate of nausea and vomiting was significantly decreased in treatment group (P =0.025).Conclusion The CAG regimen combined with thalidomide is effective with little side effects for elderly AML,and is worthy of further study and clinic application.

Hemoperfusion ; Humans ; Metabolic Clearance Rate ; Paraquat ; blood ; pharmacokinetics

This study investigated the inhibitory effects of curcumin on proliferation of hematological malignant cells in vitro and the anti-tumor mechanism at histone acetylation/histone deacetylation levels. The effects of curcumin and histone deacetylase inhibitor trichostatin A (TSA) on the growth of Raji cells were tested by MTT assay. The expression of acetylated histone-3 (H(3)) in Raji, HL60 and K562 cells, and peripheral blood mononuclear cells (PBMCs) treated with curcumin or TSA was detected by immunohistochemistry and FACS. The results showed curcumin inhibited proliferation of Raji cells significantly in a time- and dose-dependent fashion, while exhibited low toxicity in PBMCs. Curcumin induced up-regulation of the expression of acetylated H(3) dose-dependently in all malignant cell lines tested. In conclusion, curcumin inhibited proliferation of Raji cells selectively, enhanced the level of acetylated (H(3)) in Raji, HL60, and K562 cells, which acted as a histone deacetylase inhibitor like TSA. Furthermore, up-regulation of H(3) acetylation may play an important role in regulating the proliferation of Raji cells.
Acetylation/drug effects ; Antineoplastic Agents/*pharmacology ; Cell Proliferation/*drug effects ; Curcumin/*pharmacology ; HL-60 Cells ; Histone Deacetylase Inhibitors/pharmacology ; Histones/chemistry ; Histones/*drug effects ; K562 Cells ; Triclosan/pharmacology

CD44 is a transmembrane glycoprotein,which is a family of highly specific single chain mem-brane surface glycoproteins by a single gene.CD44 is mainly involved in the specific adhesion between cells and cells,cells and matrix.CD44v6 is a member of the CD44 family,and its correlation with metastasis and prognosis of non small cell lung cancer( NSCLC) has become a hot research topic in recent years.The current research pro-gress of CD44v6 and NSCLC are reviewed in this paper.

OBJECTIVE:To observe the clinical efficacy and ADR of Fuzheng huaji pills in the adjunctive treatment of mid-dle and advanced primary liver cancer. METHODS:122 patients with middle and advanced primary liver cancer who had lost the chance or were unwilling to accept surgery,radiotherapy or chemotherapy were randomly divided into control group (61 cases) and study group(61 cases). The control group received conservative treatment such as hepatoprotecives;the study group was treat-ed with Fuzheng huaji pills 1 pills per time,3 times a day,additionally for 30 days. The tumor volume changes,major clinical symptom scores,quality of life,hemorheology indexes and alpha-fetal protein(AFP),lab index and ADR were compared between 2 groups. RESULTS:After treatment,tumor stable rate of study group was 67.2%,the improvement rate of life quality 27.9%, and AFP(563.35±143.17)pg/L;the scores of liver pain,abdominal distension,loss of appetite and fatigue,and plasma viscosity, whole blood viscosity,erythrocyte sedimentation rate decreased greatly,there were significant differences,compared to control group (P<0.05). One patient in study group suffered from diarrhea,and the symptom was relieved after symptomatic treatment;no obvious ADR was found. CONCLUSIONS:Through stabling tumor, improving major clinical symptom, life quality and hemorheology indexes,Fuzheng huaji pills play a synergistic treatment effect on primary liver cancer.

Objective To investigate the effect of hyperoxia on the expressions of cysteinyl aspartate specific proteinase -3(Caspase -3)and proliferating cell nuclear antigen (PCNA)in bone marrow mesenchymal stem cells (BMSCs).Methods Primary BMSCs from SD rats were cultured in vitro,BMSCs grew to 70% -80% degrees of fu-sion and then were randomly divided into room air group and hyperoxia exposure group.Each group was divided into 5 sub -groups,and cultured for 0,3,6,1 2,and 24 h respectively.Morphology investigation with inverted phase contrast microscope was adopted.The expressions of Caspase -3 and PCNA protein levels were detected by Western blot. Results (1 )As time wenty by,compared with room air group,the gap between cells increased,some of the cells be-came circular,and cell detachment and floating appeared in the hyperoxia -exposure group (>1 2 h)compared with room air group.(2)As time went on,compared with the room air group,the expression levels of Caspase -3 protein in the hyperoxia -exposure group were increased,and the difference was significant after 6 hours of culture (0.27 ± 0.04 vs 0.1 4 ±0.02,t =5.03,P =0.007).(3)Compared with room air group,the PCNA levels of the hyperoxiaexpo-sure group(6 h)decreased,and the difference in PCNA protein expression levels was significant after 6 hours of culture (0.27 ±0.04 vs 0.38 ±0.04,t =3.37,P =0.028).Conclusions Hyperoxia exposure increases Caspase -3 expres-sion levels and decreases PCNA expression,which may affect the proliferation and apoptosis of BMSCs.