1.Clinical analysis for the diagnosis and treatment of 25 cases with primary lacrimal gland epithelial tumor
Chinese Journal of Primary Medicine and Pharmacy 2016;23(22):3472-3475
Objective To explore the clinical manifestation,imaging features and treatment of primary lacri-mal gland epithelial tumor.Methods The clinical data of 25 cases with primary lacrimal gland epithelial tumors were retrospectively studied.Results All of 25 primary lacrimal gland epithelial tumor cases received surgical treatment. Fourteen primary orbital tumors cases were male and 11 cases were female.The mean age was 44 years old (ranged 23 to 65).The mean hospital stay was 12d(ranged 7 to 20).Among 25 primary lacrimal gland epithelial tumor cases, 11 cases were benign tumors which included 4 inflammatory pseudotumor,11 pleomorphic adenoma.Fourteen cases were malignant tumors which included 4 malignant pleomorphic adenoma,6 adenoid cystic carcinoma and 4 adenocar-cinoma.After opeation,visual acuity improved in 9 cases,unchanged 10 cases,decreased 6 cases.The patients were followed up for 16 -48 months(mean 27 months).There were 4 malignant tumors recurrence after operation and received radical operation.While 2 patients were lost and 2 patients died of tumor metastasis,the other 21 patients survived with tumor -free.Conclusion Primary lacrimal gland epithelial tumors have different clinical and imaging appearances.Combination of ultrasound,CT and MRI is important to ascertain the character,range and degree of primary lacrimal gland epithelial tumors.Surgical excision is the main and effective treatment for primary lacrimal gland epithelial tumors,while gamma knife treatment is safe and effective for malignant,unresectable,recurrences tumors.
4.The Analysis of Lung Function of 558 Patients with Chronic Obstructive Pulmonary Disease
Yan CHEN ; Ping CHEN ; Zhijun LIU
Journal of Chinese Physician 2001;0(02):-
Objective To analyse the lung function parameters of the patients with chronic obstructive pulmonary disease (COPD) to explore the clinical significance of lung function in the diagnosis and evaluation of COPD. Methods The lung function of 558 patients with COPD from January 2000 to April 2003 in our hospital was retrospectively analysed by using SPSS 10 0 software. Results The average ages of the 558 patients were 57 6?9 7 years, 78 9% of which were male. Patients with grades Ⅱ and Ⅲ COPD accounted for 70 2%. There was a negative relationship between FEV 1, FEV 1/FVC and smoking index(r=-0 039,-0 305,P
5.Survey of the standardization of medical records writing by physicians
Yanmin CHEN ; Yan LIU ; Yamin LIU
Chinese Journal of Hospital Administration 2011;27(1):12-15
Objective To study the standardization of medical records writing by residents in hospitals. Methods With both quantitative and qualitative analysis, medical records made by residents were sampled in groups to study their standardization. Results Recognition rate of such medical records was 82. 55%, and the excellence rate was 24. 50% in the grade evaluation. For senior residents, their rate of writing excellence falls below those of junior ones; whether the evaluators have medical background bears no significant difference for grade evaluation of medical records writing, yet a significant difference was found with the recognition rate. Conclusion Writing of medical records by residents is found with incompliance now and then, and the recognition rate ought to be improved.
6.Significance of coronary collaterals in patients with acute coronary syndrome
Yan LIU ; Shaoliang CHEN ; Zhizhong LIU
Chinese Journal of Clinical Pharmacology and Therapeutics 2002;0(06):-
AIM: To study the relation between development of coronary collaterals and clinical outcome in patients with acute coronary syndrome(ACS).METHODS:251 patients with clinical diagnosed ACS were consecutively enrolled into the present study and divided into two groups according to the presence of coronary collaterals by quantitative coronary angiography(QCA).Serial venous blood samples were collected at admission for determining of high-sensitive C-reactive protein(hs-CRP),brain natriuretic peptide(BNP),and cardiac troponin I(cTnI).Baseline left ventricular ejection fraction(LVEF)was determined by cardiac echo.RESTULTS: Coronary collaterals were detected in 38 patients(as CC group),another 213 patients without coronary collaterals were served as control group.Serum levels of cTnI in patients in CC group was significantly higher than in control group((0.91)?(1.13) vs(0.29)?(0.23)(ng?ml~(-1)),P(0.05)).Serum levels of cTnI in patiemets of CC group was significantly lower than those of control group((0.29)?(0.23) vs(0.91)?(1.13)(ng?ml~(-1)),P
7.Long-term Follow-up for Patients with Stage Ⅰ Endometrial Cancer after Laparoscopic Surgery
Haifang LIU ; Xu CHEN ; Yan LIU
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To compare the long-term prognosis of patients with stage Ⅰ endometrial cancer (EMC) after laparoscopic surgery or open laparotomy. Methods Totally 83 patients with stage Ⅰ EMC who underwent laparoscopic surgery or open laparotomy between 1993 and 2008 were enrolled in this study. The clinical data were analyzed retrospectively to compare the survival time during long-term follow-up between the two groups. Results The total survival rate was 95.2% (79/83) in the 83 cases. The median survival time was 77,51,and 31 months respectively in stages Ⅰa,Ⅰb,and Ⅰc EMC patients. No significant difference was found in the survival rate between open and laparoscopy groups [94.2% (49/52) vs 96.8% (30/31),Z=0.028,P=0.978]. For the stage Ⅰa,stages Ⅰb and Ⅰc,and G2 and G3 patients,the median survival time was similar between the open and laparoscopy groups (80 month vs 63 months,P=0.483; 49 months vs 48 months,P=0.781; and 51 months vs 49 months,P=0.635). However,in the G1 patients,significantly difference was detected in the median survival time between the two groups (77 vs 51 months,P=0.037). Conclusion Laparoscopic surgery should be used as a routine surgical approach for patients with stage Ⅰ EMC considering its minimal invasiveness and promising prognosis.
8.Determination of lead in workplace air by portable X-ray fluorescence spectrometry.
Juntong LIU ; Yan LIU ; Ya CHEN ; Yan ZHAO ; Tong WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(4):299-301
OBJECTIVETo investigate the permance index ofof portable X-ray fluorescence spectrometer in the determination of lead on filter membrane and to provide data for the determination of lead in workplace air.
METHODSIrradiated with X-ray, the lead would emit specific X-ray fluorescence during the process from the excited state back to the ground state. Rapid determination of lead was completed using fluorescence energy and wave length for qualitative analysis and fluorescence intensity for quantitative measurement. Under set conditions, a series of customized calibration samples were measured to create a standard curve for quantitative analysis of lead on filter membrane.
RESULTSThe regression equation obtained using a portable X-ray fluorescence spectrometer to determine the lead on filter membrane was y=0.004x-0.182 (r2= 0.9999). The linear range was 0.00 -10.40 mg/m3, the minimum detectable concentration was 0.53 µg/m3, and the minimum quantifiable concentration was 1.76µg/m3. The relative standard deviation (RSD) of within-run precision of samples with different concentrations was 0.48%-6.22%, the RSD of between-run precision was 2.51%-5.09%, and the degree of accuracy was in the calibration range of standard samples.
CONCLUSIONPortable X-ray fluorescence spectrometry is a simple, rapid, repeatable, and accurate method for the determination of lead on filter membrane.
Air Pollutants, Occupational ; analysis ; Lead ; analysis ; Spectrometry, X-Ray Emission ; Workplace
9.Mechanisms of tumor necrosis factor-related apoptosis inducing ligandcombined with Triptolide in inducing the apoptosis of pancreatic cancer cells
Yan JIANG ; Wei LIU ; Zhiyu CHEN
Chinese Journal of Digestive Surgery 2015;14(4):321-328
Objective To investigate the mechanisms of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) combined with Triptolide in inducing the apoptosis of pancreatic cancer cells.Methods (1) The pancreatic cancer cells (MiaPaca-2 cells) were divided into 4 groups:blank control group (no drugs were added),TRAIL + Triptolide-group (only TRAIL was added),TRAIL-Triptolide + group (only Triptolide was added) and TRAIL+ Triptolide+ group (TRAIL and Triptolide were added).The vitality of cells in all the 4 groups was assessed by CCK-8.The expressions of poly ADP-ribose polymerase (PARP),cysteinyl aspartate specific proteinase-3 (Caspase-3) and Caspase-8 were detected by Western blot.The vitality of cells was detected by CCK-8 and the vitality of Caspase-8 was detected by Caspase-Glo assays after adding Z-IETD-FMK,a specific inhibitor of Caspase-8.The expressions of myeloid cell leukemia-1 (Mcl-1),Bcl-xL and Bcl-2 were detected by Western blot.(2) The MiaPaca-2 cells were divided into 8 groups:①TRAIL-Mcl-1 siRNA-group (no TRAIL was added and Mcl-1 siRNA cells were not transfected),TRAIL+ Mcl-1 siRNA-group (TRAIL was added and Mcl-1 siRNA cells were not transfected),TRAIL-Mcl-1 siRNA + group (TRAIL was not added and Mcl-1 siRNA cells were transfected)and TRAIL+ Mcl-1 siRNA+ group (TRAIL was added and Mcl-1 siRNA cells were transfected).②TRAIL-Bcl-xL siRNA-group (TRAIL was not added and Bcl-xL siRNA was not transfected),TRAIL+ Bcl-xL siRNA-group (TRAIL was added and Bcl-xL siRNA was not transfected),TRAIL-Bcl-xL siRNA + group (TRAIL was not added and Bcl-xL siRNA was transfected) and TRAIL+ Bcl-xL siRNA+ group (TRAIL was added and Bcl-xL siRNA was transfected).The vitality of the cells in all the groups was detected by CCK-8.The expressions of Caspase-3 and Caspase-8 protein were detected by Western blot.The measurement data with normal distribution were presented as (x) ± s.The comparison among groups was done by ANOVA,and the pairwise comparison was done by LSD-t test.Results (1) The vitalities of MiaPaca-2 cells in the blank control group,TRAIL + Triptolide-group,TRAIL-Triptolide + group and TRAIL + Triptolide + group were 100.0% ± 1.1%,81.2% ± 2.3%,78.6% ± 3.6%and 40.1% ± 2.5 %,and the relative expressions of PARP protein were 0.510 ± 0.028,0.720 ±0.072,1.250 ±0.023 and 2.560 ± 0.220,the relative expressions of Caspase-3 were 0.080 ± 0.004,0.080 ± 0.003,0.110 ±0.005 and 2.720 ± 0.003,and the relative expressions of Caspase-8 were 0.070 ± 0.003,0.080 ± 0.005,0.120 ±0.003 and 0.990 ± 0.006,with significant differences among the 4 groups (F =203.607,1 457.785,332 421.900,35 437.218,P < 0.05).The vitality of M iaPaca-2 cells in the TRAIL + Triptolide + group was significantly different from those in the blank control group,the TRAIL + Triptolide-group and the TRAIL-Triptolide + group (t =34.583,355.936,36.271,P < 0.05).The relative expression of PARP protein of MiaPaca-2 cells in the TRAIL+ Triptolide + group was significantly different from those in the blank control group,TRAIL+ Triptolidegroup and TRAIL-Triptolide + group (t =591.784,63.739,2 268.987,P < 0.05).The relative expression of Caspase-3 protein of the MiaPaca-2 cells in the TRAIL + Triptolide + group was significantly different from those in the blank control group,the TRAIL + Triptolide-group and theTRAIL-Triptolide + group (t =3 266.153,9 145.228,1 738.713,P <0.05).The relative expression of Caspase-8 protein of the MiaPaca-2 cells in the TRAIL+ Triptolide +group was significantly different from those in the blank control group,the TRAIL+ Triptolide-group and the TRAIL-Triptolide + group (t =663.953,l 432.878,327.584,P < 0.05).The vitality of caspase-8 in the TRAIL+ Triptolide+ group was 711.0% ± 5.1% before adding Z-IETD-FMK,and then the vitality of MiaPaca-2 cells and caspase-8 changed to 70.0% ± 4.8% and 73.0% ± 2.4%,with significant differences (t =17.956,55.027,P < 0.05).The relative expressions of Mcl-1 protein in the blank control group,the TRAIL + Triptolidegroup,the TRAIL Triptolide + group and the TRAIL + Triptolide + group were 1.68 ± 0.22,2.08 ± 0.11,0.73 ±0.15 and 0.58 ± 0.18,the relative expressions of Bcl-xL protein were 0.65 ± 0.03,0.47 ± 0.03,0.32 ± 0.03and 0.26 ±0.05,the relative expressions of Bcl-2 protein were 0.65 ± 0.03,0.67 ± 0.03,0.62 ± 0.05 and 0.67 ± 0.03,with significant difference among the 4 groups (F =55.178,88.683,3.411,P < 0.05).The relative expressions of Mcl-1 protein of the MiaPaca-2 cells in the TRAIL-Triptolide + group and the TRAIL+ Triptolide +group were significantly different from those of the blank control group (t =23.506,47.631,P < 0.05) and the TRAIL + Triptolide-group (t =58.457,37.115,P < 0.05).The relative expressions of Bcl-xL protein of the MiaPaca-2 cells in the TRAIL-Triptolide + group and the TRAIL + Triptolide + group were significantly different from those of the blank control group (t =38.105,42.219,P < 0.05) and the TRAIL + Triptolide-group (t =32.476,15.814,P < 0.05).The relative expressions of Bcl-2 protein in the TRAIL-Triptolide + group and the TRAIL+ Triptolide + group were not significantly different from those of the blank control group (t =4.724,1.732,P > 0.05) and the TRAIL + Triptolide-group (t =3.464,0.000,P > 0.05).(2) The vitalities of MiaPaca-2 cells of the TRAIL-Mcl-1 siRNA-group,TRAIL + Mcl-1 siRNA-group,the TRAIL-Mcl-1 siRNA + group and the TRAIL + Mcl-1 siRNA + group were 100.0% ± 2.2%,79.3% ± 1.8%,71.2% ± 3.2% and 37.3% ± 5.4%,the relative expressions of Caspase-8 protein were 0.100 ± 0.003,0.100 ± 0.005,0.100 ± 0.003 and 0.350 ±0.005,and the relative expressions of Caspase-3 protein were 0.020 ± 0.003,0.060 ± 0.003,0.020 ± 0.003 and 0.590 ±0.004,with significant differences among the 4 groups (F =136.681,2 717.391,44 471.429,P <0.05).The vitality of MiaPaca-2 cells of the TRAIL + Mcl-1 siRNA + group was significantly different from those in the TRAIL-Mcl-1 siRNA-group,the TRAIL + Mcl-1 siRNA-group and the TRAIL-Mcl-1 siRNA + group (t =33.937,20.207,26.689,P < 0.05).The relative expression of Caspase-8 protein of the TRAIL + Mcl-1 siRNA +group was significantly different from those in the TRAIL-Mcl-1 siRNA-group,the TRAIL + Mcl-1 siRNA-group and the TRAIL-Mcl-1 siRNA + group (t =216.506,433.013,144.338,P < 0.05).The relative expression of Caspase-3 protein of the TRAIL + Mcl-1 siRNA + group was significantly different from those in the TRAIL-Mcl-1 siRNA-group,the TRAIL + Mcl-1 siRNA-group and the TRAIL-Mcl-1 siRNA + group (t =329.09,458.993,987.269,P <0.05).The vitalities of MiaPaca-2 cells of the TRAIL-Bcl-xL siRNA-group,the TRAIL+ Bcl-xL siRNA-group,the TRAIL-Bcl-xL siRNA + group and the TRAIL+ Bcl-xL siRNA + group were 100.0% ± 2.3%,87.2% ± 4.1%,74.1 ± 3.7% and 56.3% ± 5.4%,and the relative expressions of Caspase-3 protein were 0.060 ±0.004,0.070 ± 0.003,0.060 ± 0.004 and 0.390 ± 0.003,with significant differences among the 4 groups (F =70.074,4 643.478,P < 0.05).The vitality of MiaPaca-2 cells of the TRAIL + Bcl-xL siRNA + group was significantly different from those in the TRAIL-Bcl-xL siRNA-group,the TRAIL+ Bcl-xL siRNA-group and the TRAIL-Bcl-xL siRNA + group (t =24.416,41.170,18.136,P < 0.05).The relative expression of Caspase-3 protein of the TRAIL + Bcl-xL siRNA + group was significantly different from those in the TRAIL-Mcl-1 siRNA-group,the TRAIL + Mcl-1 siRNA-group and the TRAIL-Mcl-1 siRNA + group (t =285.788,554.256,190.526,P < 0.05).Conclusion Triptolide could induce the apoptosis of MiaPaca-2 cells by inhibiting the expressions of Mcl-1 and Bcl-xL,sensitizing TRAIL and activating Caspase-8 and Caspase-3.
10.Infection Distribution and Drug Resistance Analysis of Pseudomonas Aeruginosa in Third District Hospital in Shenzhen Area
Qiaobin CHEN ; Aisheng LIU ; Yan WEN
Journal of Modern Laboratory Medicine 2015;(3):147-150
Objective To understand the shenzhen longhua new district and the light district three third district hospital pseudomonas aeruginosa infection the clinical distribution and drug resistance,for clinical provides the basis for scientific and medical treatment.Methods Collected 3 176 clinical specimens in three district hospital from June 2013 to November 2014 and they were done bacteria identification with VITEK-32 bacteria identification instrument of French biomerieux.For pseudomonas aeruginosa specimens using the K-B method and trace the broth dilution method (MIC)to do drug sensitive test,and the inspection results were statistically processed.Results 3 176 specimens pseudomonas aeruginosa isolated total separation rate was 51.16% (1 625/3 176),including respiratory sputum specimens was 52.8% (858/1 625),followed by bronchoalveolar lavage and pus,were 20.1% (327/1 625)and 16.7% (271/1 625).Ward,neurosurgery and thoracic sur-geons are mainly distributed in the ICU,were 41.6% (676/1 625),15.9% (259/1 625)and 19.1% (310/1 625).Carbon penicillium,resistance to carbon alkene sensitive penicillium alkene and extensive drug resistance rate of pseudomonas aerug-inosa isolated were 67.1% (1 090/1 625),31.6% (514/1 625)and 1.29% (21/1 625).Resistance to carbon penicillium al-kene the drug resistance of pseudomonas aeruginosa from penicillium carbon alkene sensitive serious,in addition to the poly-myxin B resistance to both comparative difference was statistically significant (χ2 = 12.617~ 12.617,P <0.05~0.001),2 cases of resistance to carbon penicillium alkene pseudomonas aeruginosa to polymyxin B resistance,in addition to amikacin, gentamycin,tobramycin has high sensitivity,the rest of the 11 kinds of antimicrobial drug resistance to all>60%.Conclusion Clinical pseudomonas aeruginosa had a high separation rate,mainly comes from the respiratory tract and the distribution of the ICU ward.Penicillium carbon alkene resistant pseudomonas aeruginosa than carbon penicillium sensitive resistance was serious,should pay close attention to carbon blue mould resistant pseudomonas aeruginosa resistance development,take effective measures of preventing transmission and infection of scientific use of antimicrobials,put an end to resistance to car-bon penicillium alkene and the spread of drug resistance pseudomonas aeruginosa .