Objective:To investigate the expression of serum galectin-3 (Gal-3), cystatin-C (Cys-C) and endothelin-1 (ET-1) in elderly patients with hypertension and analyze their correlation with left ventricular hypertrophy(LVH).Methods:A total of over 65 years old 110 patients with essential hypertension in Tieling Central Hospital from July 2018 to May 2019 were selected as hypertension group, according to the LVH, patients with essential hypertension were divided into (non LVH group, 66 patients) and LVH group (44 patients). At the same time, a total of 110 healthy people in the same period were selected as control group. The expressions of serum Gal-3, Cys-C and ET-1 in each group were compared, and the correlation between left ventricular mass index (LVMI) and Gal-3, Cys-C in patients with essential hypertension were analyzed.Results:The levels of Gal-3, Cys-C and ET-3 in the hypertension group were higher than those in the control group: (132.28 ± 38.18) μg/L vs. (110.60 ± 17.51) μg/L, (1.19 ± 0.12) mg/L vs. (0.81 ± 0.06) mg/L, (21.28 ± 0.72) ng/L vs. (16.50 ± 0.68) ng/L, and the differences were statistically significant ( P<0.05). The levels of Gal-3, Cys-C and ET-1 in the LVH group were significantly higher than those in the non LVH group: (159.75 ± 36.37) μg/L vs. (113.96 ± 26.79) μg/L, (1.24 ±) mg/L vs.(1.16 ± 0.08) mg/L, (21.84 ± 0.71) ng/L vs.(20.90 ± 0.41) ng/L, and the differences were statistically significant ( P<0.05). Pearson correlation analysis showed that LVMI was positively correlated with Gal-3( r=0.650, P<0.01), Cys-C( r=0.659, P<0.01) and ET-1( r=0.858, P<0.01). The receiver operating characteristic(ROC) curve showed that the area under the curve (AUC) of Gal-3 in diagnosing hypertensive LVH was 0.821, 95% CI 0.743-0.899, the cutoff value was 148.55 μg/L, and the sensitivity and specificity were 59.10% and 89.40%. The AUC of Cys-C in diagnosing hypertensive LVH was 0.672, 95% CI 0.558-0.786, the cutoff value was 1.320 mg/L, and the sensitivity and specificity were 43.20% and 98.50%. The AUC of ET-1 in diagnosing hypertensive LVH was 0.876, 95% CI 0.811-0.941, the cutoff value was 22.275 ng/L, and the sensitivity and specificity were 65.90% and 95.50%. The AUC of combined detection was 0.948, 95% CI 0.855-0.976, and the sensitivity and specificity were 84.10%, 98.50%. Conclusions:The serum levels of Gal-3, Cys-C and ET-1 in elderly patients with hypertension are significantly increased, and there are a good correlation between them and LVH, and combined detection can be used as a sensitive index for the diagnosis of hypertensive LVH.

In recent years,traditional English teaching has been greatly affected by network platform.As SMMU has been trying to perfect the Online English Writing Course,the questionnaire completed by the students taking the course shows that the course designs have proved effective in the improvement of research-based learning.

Objective To investigate on the students' network usage,addiction and influencing factors at a certain university and analyze its correlation with impulsive choice.Methods Multi-class sampling method was applied.1 777 students were selected from the School of Clinical Medicine,School of Nursing,School of Public Health,School of Mental Health,School of Medical Laboratory Science,Department of Stomatology,School of Pharmacy,School of Management and Department of Biology,including 1 534 medical majors,accounting for 86.3％ and 243 non-medical majors,accounting for 13.7％.Results 121 students were addicted to the internet,accounting for 6.8％ of the selected students,while 1 656 students were free from the internet addiction,accounting for 93.2％ of the selected students.Among all the addicts,96 were from medical majors,accounting for 79.3％ and 25 were from non-medical majors,accounting for 20.7％.As for the online activities,boys loved computer games while girls preferred to watch movies,listen to music and shop.At the same time,most of the junior students and non-medical majors played games online.As for the length of time,students spending 5 and more hours everyday on the internet accounted for 3.4％.As for the sites,medical majors usually used their cell-phones while non-medical majors mostly got on line at their dorms.Probably due to the boys' preference for computer games,a larger proportion of boys getting on line at school dorms and internet bars off the campus.Most of the girls chose to use their cell-phones.More junior students surferred the internet at dorms while more senior students used their cell-phones.As for the function of the medical knowledge on the prevention and treatment of internet addiction,9.8％ of the students held that medical knowledge had a great effect on the prevention and treatment of internet addiction,61.5％ believed in a general effect,while 28.6％ of the students thought it useless.The multi-factor analysis showed that grade,home address and the communication with parents all attribute to internet addiction.Internet addiction was significantly associated with impulsiveness,and 3 dimensionality of impulsive choice (P＜ 0.01).Conclusion There is higher incidence of internet addiction among the junior students,students from the urban areas and those who lack communication with parents.Grade,home address and situation of communicating with parents are the important factors for internet addiction.Internet addiction is close association with impulsive choice.

AlM: To compare the anterior segment measurements derived from optical low coherence reflectometer ( Lenstar LS900 ) and combined Scheimpflug - Placido disk topographer ( Sirius) .
METHODS: ln this study, we enrolled healthy myopic subjects 54 ( 87 eyes ) . The central corneal thickness (CCT), anterior chamber depth (ACD), flat keratometry ( FK) readings , steep keratometry ( SK ) readings and white to white ( WTW ) were measured by LS900 and Sirius. Evaluation and analysis were performed using paired t tests, the Pearson correlation, and Bland-Altman analyses.
RESULTS: The CCT and ACD measurements were significantly lower whereas FK, SK and WTW measurements were higher with LS900 (P<0. 001). Mean differences were -6. 11 ± 6. 32μm,-0. 09 ± 0. 07mm, 0. 18 ± 0. 25D, 0. 21 ± 0. 36D and 0. 25 ± 0. 39mm with statistical significant (P<0. 01). Pearson correlation analysis showed high correlation between the 2 devices for all measurements (P<0. 001). On Bland-Altman analysis, 95% limits of agreement for all measurements were 6. 26 to -18. 49μm; 0. 04 to 0. 22mm; 0. 68 to 0. 32D; 0. 92 to 0. 50D and 1. 00 to 0. 5mm.
CONCLUSlON: Anterior segment parameters evaluated with LS900 and Sirius systemare correlated well and achieve good agreement. However, there are significantly statistical differences which may be caused by the different measurement modes, so they may not be interchangeable use under certain clinical circumstances.

AIM： To estabish a HPLC assay for the determination of pirarubicin（Pir） in plasma.METHODS： Daunomycin（DM） was used as the internal standard.Plasma samples were extracted with CHCl3∶ CH3OH（2∶ 1） .0.4M NH4Cl buffer（pH=9.0） solution： CH2OH（1∶ 9） and the internal standard were added.Separation was carried out on a 250mm× 4.6mm（5μ m） phenomenex column with 0.04M KH2PO4（pH=3.0） ∶ CH3CN（68∶ 32） as mobile phase.Fluorescent detector was set at an excitation wavelength of 480nm and an emission wavelength of 550nm.RESULTS： The calibration curves for serum Pir was linear over the range of 5～1 000ng/ml（r=0.9 997） .The recovery of Pir was 95.3％ .The within-day and between-day variations were less than 5％ .T1/2β ， CLs， Vd and AUC of Pir were（12.8± 5.9） h， （128.3± 52.6） L/（m2· h） ， （1 754.3± 478.2） L/m2 and （428.7± 137.2） ng/（h· ml） ， respectively.CONCLUSION： The method is suited for monitoring blood concentration and pharmacokinetic study of Pir.

Objective To investigate the expression of thiredoxin domain containing 5 (TXNDC5) in the synovial tissues and blood samples of various arthritic conditions and autoimmune diseases to further confirm the previous findings, investigate the relations between the expression level of TXNDC5 and clinical parameters of RA. Methods The expression of TXNDC5 in the synovium was quantitatively analyzed by immunohistochemistry, real-time quantitative PCR and Western blotting. The levels of TXNDC5 in blood and synovial fluid was determined using sandwich ELISA in patients with RA, osteoarthritis (OA), systemic lupus erythematosus (SLE), ankylosing spondylitis (AS) and normal controls. One-way ANOVA, LSD test and Spearmen' s correlation were used for statistical analysis. Results Immunohistochemistry indicated that TXNDC5 expression was significantly higher in the synovial tissues of RA (100%, 40±9) than in those of OA and AS(200%,4±4). Real time PCR and western blotting confirmed the above findings (P<0.01). Sandwich-ELISA indicated significantly elevated level of TXNDC5 in the blood and synovial fluid of patients with RA (A=1.31±0.37), but not in those of OA, SLE, and AS, the healthy controls (P<0.01). The level of TXNDC5 in the blood of RA patients (A=0.8185±0.299) was positively correlated with the level of anti-CCP (r=0.350, P =0.027). Conclusion The results suggest that the pronounced increase of TXNDC5 expression may stimulate synovial pannus formation in the hypoxic environment of RA.

ObjectiveTo investigate the expression of ATA1 in the synovial tissue from patients with ankylosing spondylitis (AS) and to localize expression of ATA1 in AS synovial membranes.In addition,tag SNPs were genotyped to determine the possible association of this gene with AS risk.MethodsWestern blotting analysis was applied to determine the expression of ATA1 in the synovial tissues by comparing the expression profiles of AS(n=8),rheumatoid arthritis(RA,n=9) and osteoarthritis(OA,n=9) samples.Immuno-histochemistry was used to localize the expression of ATA1 in the synovial membrane.The levels of ATA1 in the synovial fluid of patients with AS were determined using ELISA with OA and RA as controls.Taqman method was used to genotype tag SNPs (rs2753934,rs2749531 and rs6575424) in 56 AS cases,260RA cases and 160 healthy controls.ANOVA,LSD test andx2 test were used for statistical analysis.Results Increased expression of ATA1 in synovial membranes of AS was found when compared with samples from RA and OA.ELISA results showed significantly elevated level of ATA1 in the synovial fluid of patients with AS (1.6+0.6),but not in samples of RA(1.4±0.5) and OA (1.2±0.5)(P＜0.05).Haplotype analysis did not reveal a haplotype association in AS or RA(P＞0.05).ConclusionThe current findings suggest that upregulation of ATA1 may play an important role in the pathogenesis of AS.

Objective To explore the relationship between osteoporosis knowledge and activities and fear of falling of elderly in the community.Method 420 elders in community were investigated with osteoporosis knowledge assessment tool (OKAT) and the modified survey of activities and fear of falling in the elderly (MSAFFE).Result The overall average score of MSAFFE was (25.36±4.58),and exercise capacity dimension scored higher (13.89±2.41).The overall average score of OKAT was (9.11±3.81).The correlation coefficient of MSAFFE and OKAT was-0.11～ 0.58(P＜0.05).The level of knowledge osteoporosis could explain 24％,18％,16％ variation of activities and fear of falling in each dimension.Conclusion The level of knowledge osteoporosis in the elderly is closely related to activity and fear of falling.Health education should be strengthened to improve the life quality of older people.

Our study investigated the host cell protein which can interact with SARS-CoV N protein, and explored the functional connections. The eukaryotic expression vectors pEGFP-N1/SARS-CoVN and pdsRed2-N1/ CXCL16 were constructed and used to co-transfect HEK293FT cells by the calcium phosphate method. The HIS-tagged fusion protein SARS-CoVN-GFP was then built and purified for the binding assay in vitro. The co-localization of SARS-CoVN and CXCL16 in the cytoplasm of HEK293FT cells was also shown using confocal laser scanning microscopy. It is suggested that their interaction might be through direct combination. Under a fluorescence microscope, it was observed that the purified fusion protein SARS-CoVN-GFP was attached to the cell membrane of CXCL16-transfected cells, indicating that SARS-CoVN and CXCL16 can be mutually combined.

Objective To explore the effects of improved preparation before urodynamic examination in the elderly patients with voiding disfunction.Methods Totally 424 elderly patients underwent urodynamic examination after improvement of the preparation approach according to pathophysiological characteritics of the elderly patients,and 248 elderly patients before the approach improved in our center were collected as control.The compliance,discontinuation and repetition rate of urodynamic examination were compared between the two groups.Results The compliance of patients who used the improved preparation approach was significantly higher than control (92.5％ vs.59.3％,x2=106.428,P＜0.001).Whereas the discontinuation and repetition rates of urodynamic examination in the improved group were decreased as compared with control group ( 1.2％ vs.6.9％,x2 =14.176,P=0.0002; 8.5％ vs.21.4％,x2 =21.487,P＜0.001).Conclusions The improved preparation approach according to the pathophysiological characteritics of elderly patients is worthy to popularize in the urodynamic centers.