Objective To compare the clinical efficacy of manipulative reduction and splint external fixation and open reduction and internal fixation in the treatment of fractures of the proximal humerus .Methods 62 patients with proximal humeral fractures were randomly divided into the two groups .31 cases in the manipulation group re-ceived manual reduction and splint external fixation;31 patients in the open reduction and internal fixation group were treated by open reduction and internal fixation .The clinical efficacy was compared between the two groups .Results The average healing time of the manual reduction group was (98.34 ±0.42) d,which was shorter than (121.53 ± 0.45)d of open reduction and internal fixation group (t=209.76,P<0.05).The incidence rate of pain in the manu-al reduction group was 22.58%,which was lower than 48.39% in the open reduction and internal fixation group (χ2 =4.51,P<0.05).The excellent and good rate of the manual reduction group was 96.77%,which was higher than 80.65%of the open reduction and internal fixation group (χ2 =4.03,P<0.05).Conclusion The clinical curative effect of manual reduction and splint external fixation in the treatment of proximal humerus fractures is better than open reduction and internal fixation ,it is a reliable choice for treatment of fractures of the proximal humerus .

Objective To investigate the clinical effect of alendronate sodium on bone metabolism and bone mineral density (BMD) for treating the elderly female with osteoporotic distal radius fractures.Methods Ninety elderly female with osteoporotic distal radius fractures patients were divided into treatment group and control group with 45 cases each according to the method of random number list.The two groups were given the medication treatment after 3 d of external fixation stenting.The control group was given calcium dimension D and the treatment group was given alendronate sodium and calcium dimension D.The course of treatment was 6 months.Before and after treatment,the BMD and bone metabolism [bone alkaline pbosphatase(BALP),tartrate-resistant acid phosphatase-5b(TRACP-5b)] were detected in both groups.The wrist function of two groups was evaluated.Results Before treatment,the level of BMD of treatment group and control group were (0.472 ± 0.157),(0.519 ± 0.173) g/cm2,there was no significant difference (P ＞0.05).After treatment,the level of BMD of treatment group and control group were (0.893 ±0.298),(0.571 ± 0.184) g/cm2,the BMD of treatment group after treatment was significantly higher than that before treatment and control group after treatment(P ＜ 0.05).The level of BMD of control group after treatment had no significant difference compared with before treatment (P ＞ 0.05).Before treatment,the level of BALP,TRACP-5b of treatment group and control group were (53.76 ± 17.92),(5.61 ± 1.87) U/L and (51.86 ± 17.29),(5.54 ± 1.85) U/L,the level of BALP,TRACP-5b after treatment had significant difference compared with before treatment and control group after treatment (P ＜0.05).There was no significant difference in control group before treatment and after treatment (P ＞ 0.05).The good rate of rehabilitation of wrist function in treatment group was 91.1％ (41/45),significantly higher than that in control group [51.1％ (23/45)] (x2 =17.524,P ＜0.01).Conclusions The alendronate sodium has a role to suppress bone resorption and increase BMD.It can promote the recovery of wrist function.

OBJECTIVEThe aim of this study is to establish a transgenic mouse model for cleft palate relevant gene thyroid transcription factor-2 (TTF-2), which can be used to study palatal shelf development when the expression pattern and regular activation of TTF-2 is altered.

METHODSThe C57BL/6J mouse TTF-2 gene was cloned through polymerase chain reaction (PCR) from the mouse genomic DNA. The TTF-2 gene was inserted into the expression vector pBROAD3-mcs to construct the recombinant expression vector pBROAD3-TTF-2. This expression vector was then microinjected into the male pronuclei of the fertilized mouse ovum. Thus, the TTF-2 transgenic mice model was established. The genotype of the transgenic mice was identified by PCR and Southern blot analysis. Immunohistochemistry identified the consistent expression of TTF-2 gene during its palatal shelf development.

RESULTSTTF-2 genes were microinjected into 982 fertilized ova. A total of 580 two-cell-stage embryos cultured and transplanted into the oviducts of 48 pseudopregnant female mice. Overall, 68 embryos were obtained for analysis. The genotype of the mice was determined through PCR and Southern blot analysis using genomic DNA extracted from tail biopsies of the transgenic fetus. A total of 13 TTF-2 transgenic mice were detected. The expression of TTF-2 gene during the palatal shelf development of the transgenic mice was consistently detected by immunohistochemistry.

CONCLUSIONThe recombinant expression vector pBROAD3-TTF-2 was integrated into mouse genome through microinjection. The transgenic mouse in the palatal shelf that consistently expressed TTF-2 was successfully established and displayed a cleft palate phenotype.

Animals ; Cleft Palate ; Disease Models, Animal ; Female ; Forkhead Transcription Factors ; Genotype ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Thyroid Gland

AIM:To detect the changes of cystathionine β-synthase (CBS) expression in the brain following ischemia-reperfusion of hind limbs in the rats and to elucidate their significance .METHODS:Hind limb ischemia was in-duced by clamping infrarenal aorta with a microvascular clip and brain injury was made by following reperfusion .The brain tissue was obtained from the animals subjected to sham operation , 4 h of ischemia without reperfusion and 3 h, 6 h, 12 h, 24 h of reperfusion following 4 h of ischemia .The expression of CBS at mRNA and protein levels was measured at different time points by reverse transcription-polymerase chain reaction ( RT-PCR) and Western blotting .The CBS activity and hy-drogen sulfide ( H2 S) concentration in the brain were determined by a universal microplate reader .The observation of path-ologic changes of the brain was made following the inhibition of CBS by hydroxylamine .RESULTS:The relative expression of CBS at mRNA and protein levels in IR group significantly increased compared with sham group .It reached a peak at 12 h (P<0.01), and returned to baseline at 24 h (P>0.05).This time course correlated with increased CBS activity and H2 S concentration .No statistically significant difference in the above indexes between sham operation group and ischemia group was observed (P>0.05).Inhibition of CBS activity induced more severe brain injury in IR group .CONCLUSION:Up-regulation of CBS/H2 S pathway is involved in the protection for neurons during the ischemia-reperfusion of hind limbs .

Objective: To further understand the role of folic acid supplements rivaling MTHFR gene silencing in pathogenesis of NCLP, RNA interference (RNAi) was applied to knock down MTHFR in mouse embryonic palatal mesenchymal (EPM) cells. Methods: MTHFR ShRNA expression vector were transfected into the primary cultured EPM cells. MTT was used to observe cell proliferation after MTHFR gene silencing. FCM was used to observe cell cycle after MTHFR gene silencing. Results: The results showed the cells proliferation had an inequality amelioration after using folic acid supplements in MEPM cells with MTHFR gene silencing. Using folic acid supplements rivaled the effect of MTHFR gene silencing had a dose-dependent manner. Using 20 μg/ml folic acid supplements could improve the cell proliferation to achieve normal level of cell proliferation. Conclusion: MTHFR gene is an important candidate gene of NCL/P. Using folic acid supplements could prevent teratogenic MTHFR gene silencing for embryonic palate development.

Objective To investigate the effects of probiotics on the occurrence of ovalbumin (OVA) induced food allergy and the regulatory effects on immune function of rat models. Methods Thirty female Brown-Norway rats aged 3 weeks were randomly divided into blank control group,food allergy group and probiotics intervention group(n=10).The levels of serum OVA-IgE and intestinal sIgA were measured by ELISA method.Splenic lymphocytes were isolated and cultured in vitro,and the Treg lymphocyte subgroups in the spleen were analyzed by flow eytometry.The levels of IFN-γ,IL-4 and IL-10 in the supernatant of cultured splenic lymphocytes were measured by ELISA method. Results The serum OVA-IgE level in food allergy group was significantly higher than that in blank control group(P<0.05),while that in probiotics intervention group was significantly lower than that in food allergy group(P<0.05).Compared with food allergy group and blank control group,the level of intestinal sIgA in probiotics intervention group was significantly higher(P<0.05).The percentage of CD4~+CD25~+T lymphoeytes in food allergy group was significantly lower than that in blank contml group(P0.05).The levels of IL-4 and IL-10 in food allergy group were significantly higher than those in blank control group(P<0.05).Probiotics intervention group had significantly lower levels of IL-4,IL-10 and IFN-γ than food allergy group(P<0.05).The ratio of IFN-γ/IL-4 in food allergy group was significantly lower than that in blank control group(P<0.05),while that in probiotics intervention group was significantly higher than that in food allergy group(P<0.05). Conclusion Probioties intervention could prevent the occurrence of food allergy in animal models by modulating the Th1/Th2 eytokine balance.

Objective To establish the quality control method for Antenoron filiforme. Methods Antenoron filiforme was identified by TLC,and the content of gallic acid was determined by HPLC. The chromatographic conditions were as follows:Aichrom Reliasil C18 column (4. 6 mm×250 mm,5 μm) was used with mobile phase consisted of acetonitrle-0. 1% phosphoric acid(4∶96),the flow rate was 1. 0 mL·min-1 ,and the detection wavelength was 270 nm. Results TLC identification for An-tenoron filiforme was highly specific. Gallic acid content had a good linearity in the range of 0. 08-0. 56μg (r=1. 000 0). The av-erage recovery was 101. 12% and RSD was 2. 20% (n=6). Conclusion The method is simple, feasible,and reproducible, and can be used for the quality control of Antenoron filiforme.

Objective To observe the clinical efficacy of Tiao Ren Tong Du (regulating the Conception Vessel and unblocking the Governor Vessel) needling method plus bloodletting at the Jing-well points in treating post-stroke spastic state. Method Sixty-seven eligible patients were randomized into a group to receive Tiao Ren Tong Du needling plus bloodletting at the Jing-well points (treatment group) and a rehabilitation group (control group). For the two groups, the treatment was given once a day, 6 times as a treatment course, for 4 courses in total. The Modified Ashworth Scale was used to evaluate the spasticity intensity, and the Patient Reported Outcome (PRO) for post-stroke spastic hemiplegia was used to measure the patients from the following four aspects:subjective symptoms, objective symptoms, mental state, and social function. Result After intervention, the scores of spasticity, and physiological, mental, social, and environmental factors were significantly improved in both groups (P<0.01,P<0.05). Compared to rehabilitation, Tiao Ren Tong Du needling plus bloodletting at Jing-well points can more significantly release the muscular spasticity and reduce the PRO score (P<0.01). The recovery plus markedly-effective rate was 70.6% in the treatment group versus 42.4% in the control group. Conclusion Tiao Ren Tong Du needling plus bloodletting at Jing-well points can effectively improve post-stroke spastic state and the quality of life.

AIM To evaluate whether l glutamine monofluorophosphate(MFP) together with Alendronate sodium may further increase bone mass in ovariectomized rats. METHODS Forty two 3 month old Sprague Dawley female rats were randomized into six groups:group 1 rats were sham operated(Sham),group 2 rats were ovariectomized controls(Ovx),and groups 3～6 were ovariectomized and received either 1 mg?kg -1 ?d -1 of alendronate sodium ,270 mg?kg -1 ?d -1 of calcium gluconate,5 6 mg?kg -1 ?d -1 of MFP or combination of 5 6 mg?kg -1 ?d -1 of MFP and 1 mg?kg -1 ?d -1 of alendronate sodium for 3 months. All animals received double bone fluorochrome labeling prior to sacrifice. At the end of experiment,the left tibiae were havested for histomorphometrical evaluations. RESULTS Alendronate sodium increased trabecular bone volume significantly with suppressed bone resorption and bone formation. Administrated calcium gluconate had no influence on the bone mass. MFP also could not restore cancellous bone of ovariectomized rats. Administration of both MFP and alendronate sodium increased bone mass significantly but did not increase bone mass compared with alendronate sodium. CONCLUSIONS The results indicated administration of both alendronate sodium and MFP could not further increase bone mass of ovariectomized rats.

Objective To develop a software through which transfusion labels can be created and printed automatically based on No.1 Military Medical Project,in order to resolve the problems of time-and-labor-consuming and errors due to transcription. Methods Based on No.1 Military Medical Project,the software of creating and printing transfusion labels was developed using PB computer programming language. Results The software can effectively avoid the errors due to copying transfusion labels by hand and save much time and effort. It optimizes nurses' working procedure. Conclusion The software is very practical for all the hospitals using the No.1 Military Medical Project.