1.Strengthening a dental gypsum model by infiltration of cyanoacrylate.
Yan WEI ; Yuan GAO ; Jin LÜ ; Bin WANG ; Jinsong LIU
West China Journal of Stomatology 2014;32(3):229-232
OBJECTIVETo explore a simple but novel method of strengthening gypsum material by cyanoacrylate infiltration. To evaluate the influence of cyanoacrylate on the mechanical properties of dental gypsum models.
METHODSGypsum specimens were polished to the dimension of 35 mmx4 mmx4 mm. Butyl-cyanoacrylate was diluted with chloroform at different concentrations, namely 20% and 30% cyanoacrylate. Gypsum specimens were infiltrated by diluting one component of cyanoacrylate at different concentrations for 8 h and then dried for analysis. The changes in elastic modulus, fracture toughness, compressive strength, biaxial strength, brinell hardness were measured. The data were analyzed using software OriginPro 8.
RESULTSThe viscosity measurements indicated that diluted cyanoacrylate were Newtonian fluids and the viscosity increased slightly within the 48 hours of preparation but still similar as water at room temperature, which could be used to infiltrating gypsum. The gypsum infiltrated with cyanoacrylate exhibited good physicochemical properties. The biaxial strength, fracture toughness, compressive strength and brinell hardness of the gypsum were improved by 39%, 30%, 63% and 18%, respectively.
CONCLUSIONCyanoacrylate can significantly improve the strength of gypsum model which indicates the potential clinical application.
Calcium Sulfate ; Cyanoacrylates ; Dental Models ; Hardness
2.Effect of vitamin E on detrusor protection in BOO rabbits
Bin JIN ; Xiaofeng WANG ; Yaming CHU ; Zheng YAN ; Xiaodong ZHANG
Chinese Journal of Urology 2006;0(S1):-
Objective To explore the protective effect of vitamin E on the rabbit bladder after partial outlet obstruction artificially setup. Methods A total of 28 New Zealand white male rabbits were divided into 4 groups (group A in 6,group B in 6,group C in 8 and group D in 8).Group A,B and C were fed a regular diet,and group D were placed on a diet enriched with 600 mg vitamin E.After 4 weeks partial outlet obstruction was created in groups C and D,while group B underwent sham operation. After 4 weeks of obstruction each rabbit was sedated and cystometry was repeated.After cystometry the bladder was weighed.The gene expression of sarcoplasmic endoplasmic reticulum,calcium,magnesium,adenosine triphosphatase (SERCA2) in bladder was detected by using RT-PCR assay,while the protein level of SERCA2 was measured by Western blot analysis. Results All parameters measured were approximately identical in nomal rabbits(Group A) and shum operation rabbits(group B).Thus,these 2 groups were combined as the control group(Group A and B).Partial outlet obstruction resulted in bladder weight increased significantly in obstructed groups given vehicle group C(13.07?1.71)g and those vitamin E group D(11.80?2.01)g,4-fold higher than in the control group (2.81?0.30)g(P
3.Correlation between deceleration capacity of heart rate and heart rate variability in patients with hyper-tension complicated coronary heart disease
Jianhua ZHOU ; Changxia CHENG ; Bin WANG ; Yan LIU ; Haixia JIN
Chinese Journal of cardiovascular Rehabilitation Medicine 2014;23(2):152-154
Objective:To explore the correlation betWeen deceleration capacity of heart rate (DC)and heart rate var-iability (HRV)in patients With essential hypertension (EH)complicated coronary heart disease (CHD).Methods:DC and HRV Were monitored in 81 patients With EH + CHD (EH + CHD group)and 80 healthy volunteers (healthy control group)respectively.Results:Compared With healthy control group,there Were significant decrease in DC [(11.99±4.93)ms vs.(6.62±1.83)ms],standard deviation of normal to normal RR intervals calculated o-ver the 24 h period [SDNN,(129.94±15.22)ms vs.(63.66±12.13)ms],root-mean square of differences be-tWeen successive normal to normal intervals [rMSSD,(55.52±9.03)ms vs.(28.25±8.81)ms]and adjacent nor-mal RR interval difference > 50ms stroke accounted for a percentage of 24h total RR interval [PNN50,(13.99± 5.63)% vs.(7.29±1.11)%]of HRV in EH + CHD group,P <0.05 all;Pearson correlation analysis indicated that DC Was positively correlatedWith SDNN,rMSSD and PNN50 in EH+CHD patients (r =0.571,0.662,0.763, P <0.05 all).Conclusion:There is positive correlation betWeen deceleration capacity of heart rate and heart rate variability in patients With hypertension complicated coronary heart disease,Which can be jointly used as evidence for diagnosis and treatment.
4.Effects of plant growth regulator uniconazole on plant morphology and biomass allocation of Salvia miltiorrhiza.
Shu-rui GAO ; Zhi-gang ZHAO ; Jun-ling HOU ; Wen-quan WANG ; Yan SONG ; Bin-bin YAN ; Yan-qing JIN
China Journal of Chinese Materia Medica 2015;40(10):1925-1929
In this study, we use pot experiment to evaluate the effect of plant growth regulator on plant morphology and biomass allocation of Salvia miltiorrhiza. Different concentrations of uniconazole were supplied to S. miltioohiza by means of foliar spray. Height, breadth and stem diameter were measured dynamically, the biomass of leaf, stem, flower and fruit, root biomass and biomass ratio were also examined at the harvest time. Owing to the treatment, plant morphology showed significant changes, the height had been greatly reduced and the breadth decreased largely. Meanwhile, the biomass allocation changed too. The biomass ratio of leaf and stem had been notably reduced while the biomass ratio of root had been increased remarkably. It appears that foliar application of uniconazole during vigorous growth period in S. miltioohiza has dramatic effect on dwarfing plant and improving resistant to lodging. This measure could also be applied to condensed cultivation of S. miltioohiza to increase production.
Biomass
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Plant Growth Regulators
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pharmacology
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Plant Leaves
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drug effects
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growth & development
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Plant Roots
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drug effects
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growth & development
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Plant Stems
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drug effects
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growth & development
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Salvia miltiorrhiza
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drug effects
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growth & development
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Triazoles
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pharmacology
5.A sensitive and convenient enzyme-linked immunosorbent assay method in serum MG7 antigen detection in gastric cancer
Bin JIN ; Xin WANG ; Yan JIN ; Zheng CHEN ; Kaichun WU ; Daiming FAN
Chinese Journal of Digestion 2016;36(3):188-191
Objective To explore a highly sensitive and highly specific method to detect the serum MG7 antigen (Ag) level for early gastric cancer diagnosis.Methods The serum MG7-Ag level was detected by enzyme-linked immunosorbent assay (ELISA) method in 116 preoperative gastric cancer patients,63 postoperative gastric cancer patients,41 patients with precancerous lesion,37 patients with precancerous diseases,50 healthy individuals and 281 patients with other cancers.Meanwhile,the expression of MG7-Ag was also examined with immunohistochemistry in patients with gastric cancer or precancerous lesion.Chi-square test was used for comparing positive rates of the two detection methods.Results The positive rate of MG7-Ag determined by ELISA was 83.6%(97/116) of preoperative gastric cancer patients,45.2%(28/62) of lung cancer patients,45.5%(20/44) of rectal cancer patients,17.6% (12/68) of colonic cancer patients,14.2% (6/42) of breast cancer patients,47.6% (30/63) of postoperative gastric cancer patients,19.5 % (8/4 1) of patients with precancerous lesions,5.4 % (2/37) of patients with precancerous diseases and 0 of healthy individuals.The sensitivity of ELISA (83.6 %) was similar with that of immunohistochemistry (94.0%)(P>0.05).However,the false positive rate of ELISA (12.8 %) was lower than that of immunohistochemistry (51.3 %) (x2 =26.491,P<0.01).There was statistically significant difference in MG7 Ag expression in gastric cancer with different clinical stages (x2=15.564,P<0.01).Conclusion This ELISA method might be a non-invasive screening method for population with high risk of gastric cancer.
6.Investigation of the roles and functions of community nurses in Xi′an
Yan GUO ; Xiaomei LI ; Wenhui JIANG ; Jin LI ; Xiaoyuan GUO ; Bin ZHANG
Chinese Journal of Practical Nursing 2015;(35):2712-2715
Objective To explore the roles and functions of the community nurses in community health service centers and their main work content in Xi′an. Methods A total of 320 community nurses in Xi′an were investigated by General Information Questionnaire and revision of Community Nurses′Roles and Functions Questionnaire. Results The work often undertaken by community nurses were: health education, community prevention, providing direct care and chronic disease management, however, the hospice care, care for people with mental disorders, family planning guidance and rehabilitation nursing were seldom involved. The scores of each role from high to low were as follows:the organizers and managers, coordinators and partners, health educators, caregivers, observer and researcher and spokesman with(2.56±0.99),(2.43±0.97), (2.38±0.84),(2.34±0.87),(2.29±0.10),(2.07±0.99) points. Conclusions The job of community nurses in Xi′an includes the medical service, disease prevention, healthcare, rehabilitation and health education etc. However, the well done jobs only are limited to some of simple functions, such as health education and health guidance are done well. The work of hospice care and mental care which need more professional abilities and interpersonal cooperation need to be promoted.
7.Research Progress in Acupuncture Treatmentfor Male Infertility
Jie CHEN ; Debiao YU ; Yan YANG ; Lin CHEN ; Dongmei ZHANG ; Fuchun WU ; Bin SHAO ; Xing JIN
Shanghai Journal of Acupuncture and Moxibustion 2016;35(8):1021-1024
The incidence of male infertility increased year by year, which seriously affects family welfare and social development. This article reviews and sorts out recent years’ studies on acupuncture treatment for male infertility. The results show that acupuncture treatment has produced a good clinical effect on this disease. Some progresses have been made in clinical acupuncture point selection, operating methods, complex treatment and therapeutic mechanism. The clinical effect and the mechanism of action of acupuncture treatment for this disease have been deeply explored from symptom differentiation and classification, acupoint selection and prescription, acupoint stimulation mode, and combined acupuncture and moxibustion therapy so as to provide new ideas for clinical acupuncture treatment and basic study.
8.Correlation analysis between interleukin-18,interleukin-1β,copeptin and acute cerebral infarction
Yan ZHAO ; Xiangling MA ; Yanmei ZHANG ; Xiangdong CHEN ; Bin LIN ; Jin ZHEN ; Yinling FENG
Chinese Journal of Postgraduates of Medicine 2012;(31):4-6
Objective To investigate the dynamic changes of serum interleukin(IL)-18,IL-1β and copeptin in acute cerebral infarction(ACI),and analyze the relationship with the serious degree of ACI.Methods The levels of serum IL-18,IL-1β and copeptin were measured by Double-antibody sanduicb enzyme-linked immunosorbent assay(ELISA)in 83 patients with ACI who were admitted to the hospital within 24 h,and neurological impairment were evaluated by European stroke scale(ESS)at the 1st,3rd and 7th day respectively after hospitalization.At the same time,the patients with ACI were compared with 32 normal adults.Results The levels of serum IL-18,IL-1β and copeptin at the 1st,3rd and 7th day after hospitalization were(131.30±31.62),(168.30±28.12),(141.26±24.23)ng/L,(0.35±0.04),(0.82±0.10),(0.52±0.21)μ g/L,and(3.64±0.26),(4.18±0.53),(3.26±2.41)μ g/L respectively.There were significant differences among different times respectively(P<0.05),furthermore they were higher than those in normal adults[(119.12±27.42)ng/L,(0.21±0.08)μ g/L,(2.63±0.23)μ g/L](P<0.05).Univariate Logistic regression analysis showed that age,copeptin,IL-18,IL-1β and ESS score was the influencing factor in the serious degree of ACI.There was no significant difference in assessing the serious degree of ACI by receiver operating characteristic(ROC)curve between IL-18 and age,copeptin,IL-1 β,ESS score(P>0.05).Conclusions The serum levels of IL-18,IL-1β and copeptin are increasing after ACI,indicating that the inflammatory and immune factors may be involved in the development process of ACI.The serum levels of IL-18,IL-1β and copeptin can reflect the serious degree of ACI.
9.Risk factors, clinical and angiographic features of women aged 50 or less with coronary artery disease
Hong YAN ; Junfu BI ; Bin ZHANG ; Taiming DONG ; Handong WU ; Huimin YU ; Liju JIN
Chinese Journal of Interventional Cardiology 2014;(5):308-312
Objective To investigate the risk factors, clinical and angiographic features of women aged 50 or less with coronary artery disease(CAD). Methods One hundred and seventy-three female CAD patients comifrmed by coronary angiographic aged 50 or less were classiifed as group A, while another 494 non-CAD women aged 50 or less as group B. The differences in CAD risk factors, clinical and angiographic features between the 2 groups were analyzed. Results There were more women with diabetes, positive CAD family history, dyslipidemia or hypertension (especially diastolic hypertension) in group A than in group B. Patient in group A had higher diastolic pressures and serum glucose level than those in group B but both groups had similar body weights, systolic pressures and menopause ages. The serum total cholesterol and triglyceride levels were higher in patients in group A than those in group B while high-density-lipoprotein (HDL) cholesterol and apolipoprotein A levels were lower in group A. The low-density-lipoprotein (LDL) and apolipoprotein B were higher in group A than in group B but without signiifcance. There were more women with positive urine protein in group A than in group B. In group A, more than 50%of patients were with single diseased artery while another 15%with slight coronary artery atherosclerosis or even normal arteries. Most of the lesions were found in left anterior descending artery (LAD) and its branches. Conclusions Risk factors of CAD included diabetes, positive CAD family history, dyslipidemia, hypertension(especially diastolic hypertension) and positive urine protein in women aged 50 or less Menopause alone, without other CAD risk factors, would not lead to CAD. Single vessel disease was more commonly found in this group of patients.
10.Application of label-free quantitative proteomics in the screening for differentially expressed serum proteins in patients with atopic dermatitis
Sujun LIU ; Jian XU ; Bin ZHAO ; Huijun ZHU ; Yan SUN ; Jin XU ; Hong SHEN
Chinese Journal of Dermatology 2014;47(8):588-590
Objective To screen differentially expressed serum proteins by label-free quantitative proteomics in patients with atopic dermatitis,in order to discover serum protein markers for atopic dermatitis.Methods Serum samples were collected from 6 patients with atopic dermatitis and 6 healthy human controls.Then,the samples from the patients were pooled together,so with those from the controls.Proteins were extracted from the two joint serum samples followed by separation with one-dimensional sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE).Peptides were obtained by enzymolysis in protein gels,which were subsequently identified by high performance liquid chromatography tandem mass spectrometry.The Mascot software was used for database searching,and the Scaffold software for relatively quantitative analysis of proteins.Results Totally,76 proteins with a difference of greater than 1.5 folds were identified between the serum samples of patients with atopic dermatitis and controls.There were 50 up-regulated and 26 down-regulated serum proteins in the patients compared with the healthy controls.Among these differentially expressed proteins,26 were only detected in the sera of the patients,and 14 proteins in those of the controls.Conlusion Differences exist in the expression of serum proteins between atopic dermatitis patients and healthy people.