The initiation and development of diabetic chronic complications is associated not only with the level of blood glucose,but also with the glucose fluctuation.The damage in large artery and microvessel may be caused by glucose fluctuation.There are two causes which induce glucose fluctuation in clinical:one is postprandial hyperglyceamia with not well controlled,the other is hypoglycemia causes by unsuitable therapy.A lot of effective new types of substances were applicated in clinical,such as ?-glucosidase inhibitor,glinide-insulin secretagogus,fast-acting and long-acting insulin analogues.With these new powerful tools,there is possibility to treat diabetes more correctly and safely,and control the blood glucose in a stable and good level.

Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

The incidence of malnutrition among patients with chronic kidney disease (CKD) constantly increases despite the development of health care and dialysis techniques.New nutritional targets for CKD patients upon maintenance treatment have been developed.Before dialysis,evidences have shown that a long-term nutritional care plan,with a control of protein intake,can effectively correct metabolic disorders including proteinuria and acidosis.During the maintenance dialysis,the optimal protein and energy intakes have been recently challenged.The new classification of nutritional disorders in CKD patients may help the easily identification of protein-energy wasting.

Arthroplasty, Replacement, Hip ; trends ; China ; Hip Prosthesis ; Humans ; Prosthesis Failure

Many transcription factors, kinases and phosphorylase regulate the expression and activity of stuthmin. The abnormality of stathmin expression or its activity is closely involved in lots of pathological or phys-iological events such as cell proliferation, development of tumor. Many kinds of malignant tumors have high ex-pression of stathmin, and inhibiting the expression of stathmin protein is a new biotherapy target in tumor geneth-erapy. Moreover, stathmin protein can influence curative effect of some chemotherapeutics ,which target microtu-bular. This has some significance with regard to the guidance of clinical medication.

Humans ; Osteotomy ; adverse effects ; methods ; Scoliosis ; surgery

Humans ; Minimally Invasive Surgical Procedures ; Spine ; surgery

Community Language Learning was employed to strengthen English class cohesion and improve students performance, and positive results have been achieved with regard to classroom atmosphere, students involvement and CET-4 scores. Initial studies and experiments show that application of foreign linguistic teaching theories in the Chinese setting is feasible and rewarding.

OBJECTIVE:To study the anticoagulative and antithrombotic activity of Tongsai ruanmai tablets.METHODS:The effects of Tongsai ruanmai tablets on coagulation time, prothrombin time(PT),the fibrinogen(Fbg) content and the dissolution rate of blood clot were observed. The antithrombotic effect of Tongsai ruanmai tablets was also studied by thrombogenesis dissolution test in vivo and in vitro in rats. RESULTS: At the dose of 0.5, 1.0 and 2.0 g?kg-1,Tongsai ruanmai tablets could significantly prolong the coagulation time and PT in rats, decrease the Fbg content, increase the dissolution rate of blood clots, prolong carotid artery thrombosis formation time, shorten the length of thrombosis of rats in vitro, lessen the wet weight and dry weight of thrombosis of rats.CONCLUSION:Tongsai ruanmai tablets possess anticoagulative and antithrombotic activity.

Objective:To construct a eukaryotic vector containing human TACI-linker-BR3-IgGFc double receptor fusion gene and to investigate its expression in COS-7 cells.Methods: The standard cloning technology was employed to construct the(title) vector,which was then used to transfect COS-7 cells by lipofectamine 2000.The expression of target protein and their interaction with BAFF and APRIL protein were examined by Western blot,ELISA,and immunohistochemistry methods.Results: The expression of the fusion protein was observed in transiently transfected COS-7 cells, with the transfection efficiency being 48%.The fusion protein was detected in the supernatant.It suggested that the fusion protein interacted well with BAFF and(APRIL) proteins;the IgGFc tag did not influence the natural assembly and the biological activity of fusion proteins.Conclusion: A vector containing TACI-linker-BR3 double receptor and IgGFc fusion gene has been successfully constructed and the(subsequent) fusion protein is bioactive,which paves a way for further research on the pathogenesis of autoimmune diseases and biotherapy strategy.