[Objective] To discuss the clinical effect of nasal intestinal obstruction(IO) conduct catheterization on IO after ovarian cancer operation. [Method] Choose 25 cases of IO after ovarian cancer operation, randomly divide them into treatment group 10 cases and control one 15 cases. The control group take general nasogastric tube imbedding, the treatment group take nasal IO conduct catheterization;observe their post-operational complications and clinical cure effect. [Result] On complications in control group, 8 cases had electrolyte disturbance, with complication rate 53.33%, the part of clinical symptoms was re-lieved, the IO signs did not disappear or were reduced partly with X-ray;5 cases were cured clinical y, 3 had marked effect, 3 were effective, 4 had no effect, the total effective rate was 73.33%;for the treatment group, al cases were successful y imbedded and made decompression and drainage, there ’s no severe side effects after imbedding tube, the IO symptoms were effectively relieved;1 case was transferred to operation;6 cases were cured clinical y, 3 had marked effect, 1 was effective, 0 had no effect, the total effective rate was 100%;after cathetering, there ’s no severe complications, among which, 2 cases had electrolyte dis-turbance, the post-operation complication rate was 20%. The comparison of complication rate and clinical effective rate of both groups had difference of sta-tistical meaning. [Conclusion] Nasal IO conduct imbedding treating IO after ovarian cancer operation had more effect on relieving IO symptoms and reduc-ing complications than nasogastric tube.

Objective To investigate the expression patterns of osteopontin (OPN) , vascular endothelial growth factor-A (VEGF-A) , and hypoxia-inducible factor-1α (HIF-1α) in primary hepatocellular carcinoma (HCC) and determine the clinical significance of this differential expression profile.Methods Immunohistochemical staining of OPN, VEGF-A, and HIF-1α was carried out on primary HCC tissues from 90 patients, HCC-adjacent cirrhosis tissues from 20 of those patients, and normal liver tissues from 15 healthy controls.Correlations between expression levels and HCC clinicopathological characteristics were assessed by Spearman's correlation coefficient.Results The majority of HCC tissues showed positive immunostaining for OPN (69/90, 76.67%) , VEGF-A (64/90, 71.11%) , and HIF-1α (66/90, 73.33%) .OPN-and VEGF-A-positivity were significantly higher than the results from the cirrhosis tissues and normal tissues.HIF-1α-positivity was similar between the HCC and cirrhosis tissues, but both were significantly different from the normal tissues.The differential expressions of OPN, VEGF-A, and HIF-1α were significantly correlated with tumor thrombus, capsular integrity, tumor differentiation and stage, and metastasis (P<0.05) .Conclusion HCC tissues overexpress OPN, VEGF-A, and HIF-1α and this differential profile may be related to HCC progression.Future investigations of this triad of factors may provide novel insights into the biological characteristics of HCC and reveal important targets of molecular therapy.

Objective To investigate the expression of uPAR and VEGF-C in hepatocellular carcinoma (HCC) and its effect on the biological behavior of HCC. Methods The expression of uPAR and VEGF-C in liver tissue specimen of 33 patients with HCC was detected by immunohistochemical staining. Results The positive expression of uPAR and VEGF-C in HCC tissues was located in cytoplasma of cancer cells. The positive rates of uPAR and VEGF-C were 69.7 %(23/33) and 78.8 %(26/33) respectively in 33 cases of HCC. The expression rates of uPAR and VEGF-C were significantly higher in HCC with metastasis than in HCC without metastasis(P<0.05). But there were not significant correlation between the expression of uPAR or VEGF-C and tumor size, tumor grade, AFP level, hepatic cirrhosis. There was a close correlation between uPAR and VEGF-C in HCC(r=0.676, P<0.01). Conclusion The expression of uPAR and VEGF-C is correlated with metastasis, uPAR and VEGF-C may be signs of aggressive phenotype for evaluating treatment and prognosis of HCC. Examination of these two proteins may be helpful to .judge biological behavior of HCC.

Objective: To establish a cephalometric norms of Downs analysis for Korean population in China. Methods: 72 Korean adults in Yanbian with normal occlusion and harmonious face were selected. Lateral cephalometric radiographs were taken and analyzed with Downs analysis. The data was compared with the norms of the Korean in Seoul, Republic of Korea, which reported by Park. Results: Means, standard deviation of Downs polygon of our samples were presented. Differences between males and females were found in our samples: Facial angle in males group was smaller than that in females group. Angle of Yaix, L1 to mandibular plane, L1 to occlusal plane and U1 to AP plane in male group were larger than female group(P<0.05). Statistically significant differences were detected between our samples and Park's: Facial angle in Yanbian group was smaller than Soul group, while, Yaxis and occlusal plane was larger in Yanbian group. Angles of convexity of Yanbian males were larger than Soul males(P<0.05). Statistically significant differences were detected between our sample and Han group in Northeast China: Facial angle and AB-NP angle were larger than Han group. Angle of mandibular plane, angle of Yaix, L1 to mandibular plane, L1 to occlusal plane, angle of U1 to L1were smaller than Han group (P<0.05). Conclusion: Korean males in Yanbian district tend to have more convex profiles than females. Compared with Korean population in Seoul, Korean in Yanbian have more convex facial profile. Facial characteristics of Korean in Yanbian are between Korean population in Soul and Han population in Northeast China.

Objective To assess stress degree of osteosarcoma caregivers and explore the impact factors of stress response. Methods We used CRA scale to investigate stress state of the primary caregivers of osteosarcoma patients, the demographic characteristics, status of patients, the objective amount of care and scores for each latitude underwent multiple regression analysis. Results The pressure of caregivers from the city was relatively light, annual income more than 50 thousand yuan and with help from the families to take care patients could effectively alleviate the pressure. The caregivers came from rural areas, household income less than 20 thousand yuan, caregivers were the patients' parents, had their own job presently, with no one to help were high - pressure groups. Conclusions Caregivers of osteosarcoma patients with high-pressure need focused intervention, more help and support should be given to these population.

Objective To establish a liquid chromatographic-mass spectrometric method ( LC-MS/MS method ) for determination of dextrorphan in human liver microsome. Methods LC-MS/MS was adopted with carbamazepine serving as an internal standard.The separation was performed on Agilent ZORBAX XDB-C18 column (2.1 mm×50 mm, 3.5μm), with mobile phase consisting of 0. 05% formic acid methanol-0. 05% formic acid in gradient elution. Dextrorphan and carbamazepine were detected on multiple reaction monitoring(MRM)mode by transitions from precursor to production(m/z 258.1→199.1, 237.1→194.1). Results The linear range of dextrorphan concentration was 19.22-768 960 ng.L-1(r=0.999 8), and the lowest quantification limit was 19.22 ng.L-1.The relative recoveries were 94.02%-98.74%, and the RSDs of intra-day and inter-day were within 10%.IC50 of psoralen on CYP2D6 was 0.6μmol.L-1. Conclusion The LC-MS/MS method is proved to be rapid, sensitive and reproducible, psoralen is a strong inhibitor of CYP2D6.

Objective To observe the effect of acupuncture on post-stroke belching. Method Ninety-six patients with post-stroke belching were randomized into a control group and a treatment group by random number table, 48 cases in each group. The control group was intervened by conventional Western medication, while the treatment group was by acupuncture in addition to the medication given to the control group. The blood Cl﹣ and Ca2﹢ contents, as well as the symptoms score and occurrence of adverse reactions were compared before and after treatment. Result After intervention, the blood Cl﹣ and Ca2﹢ contents increased and the symptoms score decreased to different extent in both groups; except for the blood Ca2﹢ content, there were significant differences in comparing all the parameters between the two groups after intervention (P<0.05); there was a significant difference in comparing the occurrence of adverse reactions between the two groups (P<0.05), and there were less adverse reactions in the treatment group. Conclusion Acupuncture can effectively control the post-stroke belching and reduce the happening of adverse reactions.

Objective To explore the relationship of course experience,course engagement and learning outcomes in medical nursing.Methods Adapted Course Experience Questionnaire and Selfestablished Course Engagement Questionnaire were used to collect data of 142 nursing undergraduates of which 126 were valid.The data were analyzed by regression analysis.Results Course experience had significant impact on course engagement,t=4.621,2.477,4.538,P＜0.01 or 0.05.Course engagement had significant impact on learning outcomes,t=2.439,P＜ 0.05.Course experience had significant impact on learning outcomes,P＜ 0.05.Conclusions It is proposed that the clinical courses should provide students with good experience,increase the deep learning engagement,and reform evaluation methods to improve the students' academic achievement.

Objective: To improve the diagnosis and treatment level of secretory otitis media (SOM) of children. Method :Clinical information of 80 children with SOM were colletcted and analysed. All of the 80 cases (144 ears)were treated by myringotomy with Grommet insertion. Among them, 30 cases by adenotonsillectomy. 10 cases by palatoplasty. Secretions of 54 ears were carded out by bacterial culture. Result:80 cases were followed up for 6 to 12 mothes. 120 ears of 144 were cured (83％) ,with hearing improved. The tympanograms and hearing threshold of pure tone were found to be normal. The Gram＇s positive and negative germs of bactevial culture were 20.7％ (6/29 samples ) and 24％ (6/25 samples ) respectively. Conclusion: Children with secretory otitis media should be treated as early as posible to improve the patients＇ hearing and develope their speech.

Objective To analyze the distribution rule of TCM syndromes and syndrome elements in chronic atrophic gastritis with osteoporosis by retrospective study method, and provide evidence for TCM treatment. Methods TCM symptoms, syndromes and syndrome elements of 127 cases of chronic atrophic gastritis with osteoporosis were retrospectively investigated and statistically analyzed. Results The distribution of TCM syndromes in chronic atrophic gastritis with osteoporosis was as follows:there were 51 cases of asthenia syndrome of both qi and yin, accounted for 40.16%;28 cases of yang deficiency syndrome of spleen and kidney, accounted for 22.05%;21 cases of syndrome of qi deficiency and blood stasis, accounted for 16.54%. The distribution of syndrome elements was:there were 82 cases of qi deficiency, accounted for 64.57%;61 cases of yin deficiency, accounted for 48.03%;28 cases of yang deficiency, accounted for 22.05%;21 cases of blood stasis, accounted for 16.54%;12 cases of qi stagnation, accounted for 9.54%;6 cases of dampness-heat, accounted for 4.72%. Conclusion The basic pathogenesis of chronic atrophic gastritis with osteoporosis is deficiency of both spleen and kidney. Deficiency of kidney is mainly qi deficiency, yin deficiency, yang deficiency, or even deficiency of both yin and yang. Reinforcing spleen and kidney is the important principle of treatment.