Objective To probe into the methods of postoperative nursing care for the patient undergoing domino liver transplantation.Methods The key of postoperative care included:monitoring of vital signs,the condition of cruor and the state of drainage rigorously; keeping the balance of intake and output; taking the immunosuppressive drugs correctly; proper diet; prevention of complication;giving psychological nursing.Results The patients passed the perioperative period successfully and were all cured.Conclusions To aim at the characteristic of this operation,implement holistic nursing care,is an important link to assure the patients of domino liver transplantation to recover smoothly after the operation.

Animals ; Cells, Cultured ; Dioxins ; pharmacology ; Insulin-Like Growth Factor II ; genetics ; metabolism ; Osteoblasts ; drug effects ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Wistar

Objective To study the protective effect of basic fibroblast growth factor ( bFGF ) on endothelial function and structure of the basilar artery of atherosclerotic rats .Methods A total of forty-eight male adult Wister rats were randomly divided into the normal control , the atherosclerosis ( AS) model and the bFGF treatment groups .The AS model group and the bFGF treatment group were injected with a single dose of vitamin D 3 (6 ×105 IU/kg) and loaded with high fat diet for six consecutive weeks .The bFGF (9.5μg/kg, twice one day) was injection into the abdominal cavity after six weeks in the bFGF treatment group for two weeks , and an identical volume saline was given for the AS model group and the normal control group .After eight weeks , all the rats were sacrificed .The relaxation percentages of the isolated basilar artery in response to acetylcholine ( Ach) were detected and the pathological lesions of them were observed under a light microscope .ELISA and colorimetry assayed the content of serum VEGF and basilar arterial nitric oxide ( NO) .The basilar artery was used for primary culture of both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). The influence of bFGF on the proliferation vitality of VECs was measured in vitro with MTT assay.TRITC-phalloidin labeling the cytoskeleton microfilament of VSMCs was observed by laser confocal microscopy .Results The early AS plaques were presented after six weeks by hyper lipid foods .Compared with the AS model group , the relaxation percentage of the isolated basilar artery , the content of both serum VEGF and basilar arterial NO in the bFGF treatment group were obviously increased, but the pathologic injury of the basilar artery was significantly decreased (P<0.05).The proliferation vitality of VECs was obviously increased (P<0.05); the cytoskeleton microfilament of VSMCs was of obviously improvement .Conclusion AS may aggravate the basilar arterial injury , but bFGF may efficiently improve the arterial endothelial function and decrease the pathological lesion of the basilar artery in the AS model rats , which may promote the arterial protective effect .

Solid state fermentation involves the growth of microorganisms on moist solid substrates in the absence of free flowing water. It has gained considerable attention of late years due to its several advantages over submerged fermentation. This paper mainly introduces the characteristics and mathematical models of solid state fermentation and summarizes the development situation of various bioreactors, the design standards and applications.

Objective To observe whether leptin protects rat islet ?-cells from lipotoxicity induced by free Taity acid ( FFA). Methods Pancreatic islets were isolated from Wistar rats. Isolated islets were divided into 4 groups; ( 1 ) Control group; (2) Leptin group (murine recombinanl leptin 100?g/L) ; (3) FFA group [0. 25 mmol/L long chain fatty acid (oleate: palmitate =2: 1 ) ] ; (4) FFA + Leptin group (both leptin and FFA were added to the culture medium). Alter being cultured lor 72 hours, triglyceride ( TG) content in islets, insulin secreted under basal and glucose stimulated conditions, and the mRNA expression of fatty acid oxidase-carnitine palmityl transferase- 1 ( CFT- I ) , fatty acid synthetase-acyl-CoA carboxylase ( ACC) , peroxisome proliferator activated receptors ( PPAR)a and PPARr in islets were examined. Results ( 1 ) TC content in islets was elevated in FFA group as compared with control group ( P

Objective To investigate the effect of fluoride on expression of Runx2 mRNA and protein in bone tissue of rats. Methods Fourteen SD rats were randomly divided into two groups: control group(tap water with fluoride ＜ 0.06 mg/L), and fluorosis group(fluoride 50 mg/L in water). After 4 moths, expressions of both mRNA and protein of Runx2 in rat bone tissue were determined by RT-PCR and Western blotting. Results The results showed that the expression of Runx2 mRNA and protein in fluoride-treated bone tissue were 2.287 ± 0.261 and 0.929 ± 0.229, respectively, both of which were significantly higher than those of control group(0.995 ± 0.123,0.317 ± 0.068, t = 11.85,6.78, P ＜ 0.05). Conclusions Fluoride can increase the expression of Runx2 mRNA and protein in bone tissue of rats, and Runx2 may be involved in the pathogenesis of bone injury caused by fluoride.

Objective: To establish a method of quantitative analysis of multi-components by single-marker (QAMS) for simultaneous determining six saponins in Caulophyllum robustum. Methods: Cauloside G was used as the internal reference standard; The relative correction factors of cauloside H, leonticin D, cauloside D, cauloside B, and cauloside C in C. robustum were calculated. To achieve the QAMS, the contents of indicative saponins were calculated by relative correction factors. At the same time, the external standard method was used to detect the contents of six kinds of ingredients in medicinal materials, and feasibility of QAMS was verified. Results: The relative correction factor had good reproducibility, there were no significant differences among the calculated values of multi-evaluation and measured values of the external standard method. Conclusion: In the absence of reference substance situation, QAMS can be used for the quality evaluation of C. robustum, and the method is accurate and reliable.

OBJECTIVETo analyze the literature on power frequency electric field testing data and to propose views and suggestions for current testing.

METHODSThe literature on power frequency electric field testing data published in the previous years was searched to identify 306 articles involving 193 valid testing data. Mann-Whitney test and Wilcoxon W test were used for analyzing the testing data.

RESULTSThe classification of data was carried out according to one quarter of occupational exposure limit (1.25 kV/m), one half of the exposure limit (2.5 kV/m), and the exposure limit (5 kV/m). The structure of testing data showed a significant difference between the non-power facility group and the power facility group (P<0.05).

CONCLUSIONAs occupational hazard factors, the radiation exposure from power frequency electric field is extensive. However, the power frequency electric field testing data in actual workplaces except high-voltage power facilities are far less than the occupational exposure limit with little harmfulness. There is a phenomenon of excessive testing at present.

BACKGROUND:Our previous study has already manifested that Chinese medicine Xiaokening can effectively prevent and treat the early diabetic nephropathy. OBJECTIVE:To compare the effect of rhein and archen on the apoptosis of mesangial cells of rats cultured with high glucose. METHODS:Mesangial cells were stimulated by different concentrations of rhein and archen (20, 40 and 80 μmol/L). Morphology of apoptotic cells was observed by hematoxylin-eosin staining. Karyon apoptosis was examined by fluorescence staining of DAPI. cellapoptosis rate was detected by flow cytometry. RESULTS AND CONCLUSION:The outcome of hematoxylin-eosin staining and DAPI staining indicated that the effect of rhein was much stronger than that of archen on the apoptosis of mesangial cells in the rats cultured with high glucose. Comparison of the apoptosis rate also indicated that the rhein had a stronger effect on the earlier and the later stage apoptotic rate of mesangial cells than archen. Both rhein and archen with different concentrations can induce apoptosis of mesangial cells, but the effect of rhein is much stronger.

Objective To observe the effect of combined therapy of external point application and herbal concentrate-granules on patients with asthma (hot wheezing in TCM).Methods 60 patients suffering from onset period of bronchial asthma were randomized to a control group and a treatment group.The control group was given budesonide inhaler and theophylline sustained release tablets.The treatment group was given combined therapy of external point application and herbal concentrate-granules.The course of treatment was 10 days.To observe the value ofFEV1％,ACT scores and TCM syndrome scores etc.Results After treatment,the value of FEV 1％ [treatment group:(82.83 ± 11.35) ％,control group:(83.85 ± 16.72) ％] and ACT [treatment group:(19.86±2.32),control group:(19.66±2.54)] in both groups were markedly increased with statistical significance (P＜0.01); and the difference between the two groups were also significant; TCM syndrome scoring [treatment group:(4.27±3.65),control group:(5.05±4.14)] was notably decreased in both groups compared with the values before the treatment with statistical significance (P＜0.01); but the difference between the two groups was not significant (P＞0.05).Conclusion The combined therapy of external point application and herbal concentrate-granules can treat bronchial asthma in the acute clinical course and improve lung function.