OBJECTIVE:To put forward game strategy of drug safety regulation,and to provide reference for decision-making by drug regulation department. METHODS:Based on evolutionary game theory,evolutionary game model of drug safety regula-tion was established,and the stability of evolution during drug safety regulation was analyzed. RESULTS & CONCLUSIONS:There were four possible evolutionary game results of drug safety regulation,including to provide qualified drugs,regulation;to provide unqualified drugs,regulation;to provide qualified drugs,no regulation;to provide unqualified drugs,no regulation. The evolution direction mainly depends on the parameter values involved in the payoff matrix of drug safety regulation. The stable state of drug safety regulation is related to regulation strength,regulation cost,enterprise safety input,probability of accident,acci-dent-related treatment cost,et al. Key strategies to improve drug safety regulation include increasing punishment,improving moni-toring technology,strengthening coordination mechanism,promoting social cohabitation and improving industry self-control.

It is still a challenge for surgeons to deal with the complex abdominal wall defect.The purpose of surgical treatment is not only to restore the integrity of the abdominal wall, but also to maintain the function of the abdominal wall.Accurate classification and partition of the abdominal wall defects before operation are the base of surgical procedure for complex abdominal wall defects.Reinforcement of the abdominal wall defects with mesh, component separation technique and tissue flap technique are important methods to achieve functional repair of the abdominal wall defects.

OBJECTIVE:To provide reference for rational use of antiemetics in cancer patients. METHODS:Two thousand six hundred and sixteen pieces of medical orders of patients receiving antiemetics were collected from oncology department of our hospital via EMRS during Oct. 2015-Jun. 2016. RESULTS:Among 2 616 cases surveyed,1 301 cases conformed to inclusion and exclusion criteria,of which irrational use of drugs were found in 595 cases,760 times in total. Main manifestations were unsuitable drug selection(33.82%),irrational usage and dosage(25.26%),unsuitable drug combination(34.08%)and non-standard medication course(6.84%). The incidence of nausea in female was higher than male,with statistical sighificance(P=0.003),but gender had no significant effect on the incidence of nausea(P>0.05). Age had no significant effect on both nausea and vomiting(P>0.05). The control rate of nausea/vomiting in rational drug use group was higher than irrational drug use group. Average cost of antiemetic treatment of different emetic risk chemotherapy drugs in rational drug use group were all lower than irrational drug use group, indicating therapeutic efficacy could be guaranteed and economic burden was reduced in rational drug use group. CONCLUSIONS:The antiemetic plan should be selected rationally according to emetic risk degree of chemotherapy drugs. Patients receiving combined chemotherapy should select antiemetic plan according to highest emetic risk degree of chemotherapy drugs,and strictly master usage, dosage and duration. At the same time,treatment cost should be considered when selecting antiemetic plan to guarantee the safe, effective,economical and rational use of drugs.

Objective To research the binding specificity of new recombination toxin LHRH-PE40 and LHRH receptor on the surface of human colon carcinoma cell line Lovo and the mechanism of anti-proliferation,and determine the apoptosis.Methods Lovo cells were analysed by LHRH-PE40 marked with()~(125)Ⅰ;the cytocidal effect of the anti-tumor was evaluated by MTT assay,and the apoptotic rate was analysed by flow cytometry.Results Lovo cells had the binding of aglucone and receptor.Half lethal dose of human colon carcinoma cells Lovo with LHRH-PE40 was 0.24 mg?L~(-1).The apoptotic rate was increased when the LHRH-PE40 concentration was ranged from 0.1 to 10 mg?L~(-1)(P

The incretion secretion metrical technique is the important embranchment in clinical diagnosis presently,in which the magnetic separate enzyme immunoassay is the main technique.This paper introduces the principle and design of Chinese enzyme immunoassay instrument,and emphatically discusses the design of nonlinear data processing model.

Objective To explore the effect of osteopontin(OPN) as marker in colon cancer tissue and analyze the relationship between osteopontin and recurrence.Methods ELISA method was used to determine the serum levels of osteopontin in 58 patients with colon cancer(observation group) and 22 cases of normal subjects(control group);semi-quantity RT-PCR was used to detect the expression of osteopontin in tissues.The expression level of OPN mRNA in colon cancer tissue and serum OPN level in 23 recurrence cases and 35 non-recurrence cases one year after operation were observed.Results The OPN mRNA expression in colon cancer tissue and the serum osteopontin level in patients in observation group were higher than those in control group(P

Objective To compare the operation effect of endoscopic versus open component separation on abdominal wall reconstruction.Methods From January 2012 to December 2013,19 patients (ECST 8 and CST 11) with abdominal wall defects were repaired with biomaterials mesh.This study analyzed retrospectively the clinical information between two groups.Results No hernia recurrences were occurred during a mean follow-up of (14.96± 8.89) months.There was no significant difference in EBL,operation time,length of stay.The rate of recurrence wound complications in CST group is higher than the ECST group,but is not significant difference.Conclusions Endoscopic or open component separation with biomaterials mesh could effectively repair the abdominal wall defect and ECST definitely showed the advantage in reducing the incisional complication.But the large sample,long-term clinical follow-up should be confirmed.

Objective To observe the effect of rosuvastatin on the expression of vascular cell adhesion molecule-1 (VCAM-1) of renal tissue in patients with the early-stage diabetic nephropathy.Methods Sixty patients with early-stage diabetic nephropathy were divided into two groups by random digits table:rosuvastatin group and regular group.The patients were given 10 mg/d rosuvastatin besides the conventional treatment in rosuvastatin group.The levels of blood glucose,blood fat,renal function,high sensitivity C-reactive protein(hs-CRP),urine albumin excretion rate(UAER) and the expression of VCAM-1 were observed.Results Compared with those before treatment,the levels of total cholesterol (TC),low density lipoprotein cholesterol (LDL-C),trtglyeride (TG) showed no decrease after treatment [(5.83 ± 1.02),(3.48 ±0.60),(7.12 ±0.99) mmol/L] in regular group (P ＞0.05),but significantly decreased in rosuvastatin group [ (4.51 ± 0.95 ),( 3.18 ± 0.47),(7.02 ± 0.87 ) mmol/L ] (P ＜ 0.01 ).The levels of UAER and hs-CRP were significantly decreased in regular group and rosuvastatin group after treatment [ (78.3 ±31.7),(53.8 ± 24.9) μ g/min,and (2.15 ± 0.48),( 1.59 ± 0.55) mg/L] (P＜ 0.01 ),then rosuvastatin groupwas more conspicuous than regular group (P ＜ 0.05 ).The expression of VCAM-1 was less in rosuvastatin group than that in regular group.Conclusion Rosuvastatin can down-regulate the expression of VCAM-1 of kidney tissues in patients with the early-stage diabetic nephropathy and these indicate that rosuvastatin can reduce urine protein by inhibiting the inflammatory process.

Objective To observe the retinal toxicity of repeated intravitreal injection with bevacizumab(Avastin)in diabetic rats.Methods Forty male Sprague Dawley(SD)rats were randomly divided into normal group(Group A,10 rats)and diabetes mellitus group(30 rats).The rats in diabetes mellitus group were induced with streptozotocin injection for diabetic retinopathy model.And then randomly divided into diabetic retinopathy(DR)group(Group B,10 rats),the rats were not intervened;the left eyes of the other 20 rats were intravitreal injected with bevaeizumab 3 μ1(25 mg/m1)for 3 times as experimental group(Group C);the right eyes of the 20 rats were not intervened as experimental control group(Group D),20 days after last intravitreal injection,retinal function was measured by Flicker Electroretinogram (F-ERG);retinal vascular pattern was determined by fluorescence microscopy of ethidium bromide(EB)stained retinal flat mounts;retinal morphological changes were determined by light microscope on hematoxylin-eosin (HE) stained sections;Thy-1 and VEGF expression was measured by immunohistochemistry staining.Results F-ERG showed that-the differences of a-and b-waves-the b-wave amplitude and the Ops-wave amplitude in the implicit time between group A,B,C and D were significant (F=33.165,36.162,19.955,23.243;P=0.000);the differences of a-wave amplitude between group A,B,C and D was not significant(F=0.097,P=0v961).Retinal blood vessel pattern was normalin Group A;retinal vascular vessels were tortuous and irregularly expanded in Group B:retinal vascular vessels of Group C were regular and thinner than Group A;microaneurysm were showed in Group D.Light microscope displayed that the layers of the rat retina of Group A were regular,the retinal architectures of Group B were irregular,the retinal layers were regular in Group C,the retinal layers were irregular in Group D.Immunohistochemistry staining discovered that Thy-1 and VEGF were mainly expressed in ganglion cell layer(GCL).Conclusion Repeated intravitreal injection of bevacizumab iS toxic tO retina of diabetes mellitus rats.

Objective To determine quantity of type Ⅰ collagen and mRNA expression of decorin and transforming growth factor (TGF)- β_1 in the upper portion of the anterior vagina in women with pelvic organ prolapse (POP). Methods Transvaginat biopsies were obtained from the anterior vaginal wall in 27 POP(POP group) and 21 non-poP(control group). The concentration of type Ⅰ collagen was determined by ELISA technique. RT-qPCR was used to verify the mRNA level of decorin and TGF- β_1. Results The mean concentration of type Ⅰ collagen in the premenopausal women in POP group [(47.94 ± 6.82) mg/g total protein (TP)]was significantly lower than that in control group [(62.33±6.57) mg/g TP],in the postmenopausal women in POP group, it was (41.82±5.96) mg/g TP, significantly lower than that in control group[(61.21 ± 4.84)mg/g TP](P< 0.01). The mRNA expression of decorin was 1.61 ± 0.10 in POP group, significantly lower than that in control group 2.77±0.60 ,while that of TGF-β_1 was higher in POP greup10.86 ± 0.23 than that in control group 9.87 ± 0.92 (P < 0.05). In the postmenopausal women in two groups, there were no significant difference in the mRNA expression of decorin and TGF-β_1 (P > 0.05). Conclusions A decrease in quantity of type Ⅰ coUagen in the connective tissue of anterior vagina may compromise the tensile strength and an increase susceptibihty to prolapse. Deeorin might be involved in the pathogenesis in premenopausal women with POP. The pathogenesis of POP is muhifactorial.