Objective To investigate the impact of psoriacis on quality of life in patients with psoriasis. Methods A questionnaire interview included daily life and activities social activities and psychologic status were conducted of. Results 48 patients, 14 patients(29%) were impacted on quatity of life in heavy, 23 patiemts(48%) in middle, 11 patients(23%) in mildly no impact in zero. Conclusion The psoriasis valgaris influenced the Quality of life.

Child ; Humans ; Neoplasms

Objective To explore the clinical characteristics and treatment of methylmalonic acidemia(MMA) in order to improve our understanding of it.Methods We analyzed the clinical manifestations,laboratory examinations and treatments of 26 cases of methylmalonic acidemia in children.Results Twenty-four cases were involved in nervous system.Nine patients were involved in renal system.Eight cases of hematological involved.Liver enzyme elevated in 2 cases as well as the cardiac system were involved in 3 cases.One case was with pneumonia onset.The laboratory findings showed metabolic acidosis in 12 cases,hyperhomocysteinemia in 8 cases and remarkable elevation of urinary methylmalonic acid concentration in all cases.Some abnormalities in globus pallidus and cerebral white matter as well as diffuse cerebral atrophy were noted by the brain CT and MRI in 15 cases.Sixteen children have received therapy of vitamin B12,and supplementation of L-carnitine with restricted-protein diet.The follow-up for a period ranging from 3 months to 1.5 years( mean 8.5 months) of 15 cases with medical therapy showed a favorable outcome of nervous system improvement in 12 cases,however,2 patients died from severe metabolic acidosis.8 patients with renal involvement were normal in urine routine and renal function.Conclusion Methylmalonic acidemia has different clinical features,so early urine organic acids analysis by GC/MS method is essential Long-term and reasonable treatment after diagnosis is an effective way to improve the prognosis.

Objective To investigate the application value of warming humidification oxygen therapy in patients after tracheotomy.Methods Forty-one patients after tracheotomy were collected consecutively for this study.All patients were randomly assigned into the treatment group (21 cases)and the control group (20 cases).The treatment group adopted warming humidification oxygen therapy,and the control group adopted common airway moistening method.Moist effect judgment,viscosity of sputum,phlegm scab formation,respiratory irritation symptoms,airway mucosal bleeding,air way resistance,lung infection cases,average days in the ICU were investigated.Results There were statistically significant differences in moist effect judgment,viscosity of sputum,phlegm scab formation,respiratory irritation symptoms,airway mucosal bleeding,air way resistance,lung infection cases,average days in the ICU between the two groups.Conclusions Warming humidification oxygen therapy is more suitable to patients with tracheotomy than common airway moistening method.

Objective To investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of glutamine synthetase (GS) of cultured rat retinal Müller cells in high glucose environment in vitro.Methods Mtüller cells were isolated from retinas of 10 Sprague-Dawley rats at postnatal day three to five by trypsin digestion,and were randomly divided into six groups,including normal control group,high glucose group,high glucose +5 U/ml rhEPO group,high glucose+ 10 U/ml rhEPO group,high glucose+ 20 U/ml rhEPO group,high glucose+40 U/ml rhEPO groups.After 48 hours,the apoptosis of retinal Müller cells were assayed by terminal transferase-mediated DNA end labelling assay,and the expression levels of GS protein were detected with semi-quantitative immunocytochemistry.Results Compared with the normal control group,the cell viability and GS protein were reduced while the cell death increased in Müller cells cultured in high glucose,the difference was statistically significant (t =27.4,P ＜ 0.01).Compared with the high glucose group,rhEPO treatment reduced the apoptotic Müller cells (t=857.2,2 374.6,2 473.2,2 537.7; P＜0.01),induced the expression of GS proteins (t=3.2,18.0,22.5,26.4; P＜0.05).Conclusions rhEPO can protect Müller cells from apoptosis under high glucose condition.The mechanism may be related to its function to up-regulate the GS protein expression,promote glutamic acid cycle,and reduce the excitotoxicity effects of high concentration of glutamate.

Objective To observe the efficacy of the combination of moxibustion and pressing beans on auricular in the treatment of elderly patients with chronic diarrhea.Methods 45 elderly patients met the diagnostic criteria for diarrhea were divided into two groups,the treatment group (23 cases) received the treatment of combined moxibustion with ear pressure beans,the control group (22 cases) received traditional Chinese medicine treatment.The efficacy was observed and compared after two weeks.Results The effective rate reached 95.6％ in the treatment group,81.8％ in the control group (statistically significant difference,odds ratio 1.16).Conclusion Combined ear pressure beans with moxibustion treatment will receive a significant effect for the elderly patients with chronic diarrhea.The therapy is worth clinical application.

Aging ; Arteriosclerosis ; pathology ; Blood Vessels ; pathology ; Cellular Senescence ; Humans ; Medicine, Chinese Traditional

Acupuncture Therapy ; Adult ; Female ; Humans ; Psoriasis ; therapy

Bimolecular fluorescence complementation (BiFC) assay is an innovative approach to investigate protein interactions, based on the reassembly of protein fragments of the fluorescent proteins which directly report interactions. The fluorescent proteins tolerate circular permutation and insertions of foreign proteins with maintenance of fluorescence. So when the proteins fused to the reporter fragments interact with each other, a direct readout of the association would be given from the facilitating reassembly of the active reporter protein. Moreover, with distinct spectra difference of the fluorescent protein family members, BiFC assay is expanded to multicolor BiFC assay which enables visualization of interactions between different proteins in the same cell and comparison of the efficiencies of complex formation with alternative interaction partners. From it first reported in Molecular Cell in 2002 till now, this approach has been used on the networks of protein interaction in mammal cells, plant cells or even E.coli, and researches on transcription factors, G protein ?? dimmers, protein ubiqutination and so on.