AIM: To investigate the roles of forkhead box c2(Foxc2) in axial skeloton. METHODS: Mice lacking Foxc2 locus were produced by targeted mutation and the developmental anomalies in axial skeleton were analyzed. RESULTS: Foxc2 protein increased markedly in Myoblasts C2C12 after treating with BMP-2, and alkaline phosphatase activity and production of osteocalcin in Myoblasts C2C12 were significantly higher than that in cell lines transfecting antisense Foxc2 sequence. Expressed median cleft palatine, dysplasia of auditory ossicles and vertebral and anteroposterior facial dysplasia were found in all 15 homozygous mouse neonates with targeted mutation of Foxc2. CONCLUSION: These results suggest that Foxc2 has indispensable roles during skeletogenesis in mesoderm and cells derived from the neural crest. [

Objective To evaluate the value of serum granulocyte colony-stimulating factor(G-CSF),interlukin-8(IL-8)and white blood cell count(WBC)in detecting acute surgical bacterial infections.Methods A total of 36 patients with infections and 32 with traumatic diseases admitted in Beijing Tongren Hospital without use of antibiotics before admission were recruited,as well as 30 normal healthy controls.Three milliliters of peripheral blood were collected from each of them.Serum G-CSF and IL-8 were measured by enzyme-linked immunosorbent assay(ELISA).Results Of all the 68 patients,serum G-CSF Was(82±71)ng/L in average in those with infections,significantly higher than that in those with trauma(15±7)ng/L(P＜0.01)and controls(7±4)ng/L(P＜0.01).Conclusions Serum G-CSF may be used as a sensitive indicator for acute surgical bacterial infections.

Objective:To compare the preventive effects of dezocine or parecoxib used alone or combination on emergence pain and agitation in the patients undergoing radical hysterectomy. Methods: Sixty ASA Ⅰ~Ⅱ patients undergoing radical hysterectomy were randomly divided into three groups. At the time of sewing incision, the dezocine group (group D, n=20) received dezocine in-travenous injection at the dosage of 0. 1 mg·kg-1, the parecoxib group (group P, n=20) received parecoxib intravenous injection at the dosage of 0.8 mg·kg-1, and the combination group (group DP, n=20) received 0.1 mg·kg-1dezocine and 0.8 mg·kg-1 parecoxib. When the operation was finished, the patients were transferred to the recovery room with endotracheal tubes, and recovered and extubated without the administration of reversal agents. Visual analogue scale ( VAS) for pain and Aono' s four-point scale for e-mergence agitation ( EA) were measured. The recovery time, extubation time, VAS, degree of EA and side effects such as nausea, vomiting, respiratory depression and hypersomnia during the emergence were also evaluated and recorded. Results:There were no sig-nificant differences in recovery time and extubation time among the three groups (P >0. 05). The VAS score and degree of EA in group DP were lower than that in group D (4. 65 ± 1. 69) and group P (5. 95 ± 1. 82) (P<0. 05), and the VAS score in group D was lower than that in group P (P<0. 05). The incidence of moderate and severe pain during the emergence in group DP (20%) was low-er than that in group D and group P (75% and 85%, respectively, P<0. 05), and that was lower in group D when compared with that in group P(P<0. 05). There were no significant differences in the incidence of degree of EA higher than 3 among the three groups (P>0. 05), and no side effects such as nausea, vomiting, respiratory depression and hypersomnia were detected during the emergence. Conclusion:Intravenous injection of 0. 1 mg·kg-1 dezocine combined with 0. 8 mg·kg-1 parecoxib at the time of sewing incision shows effective analgesia and emergence agitation reduction without obvious complications in the patients undergoing radical hysterecto-my.

The paper analyzes the challenge of big data environment to the traditional medical information services,presents the practical significance and basis of constructing the medical information service platform,introduces the construction objectives,architecture,function feature of the platform,describes the medical information service mode based on the digital platform,including medical information resource services,intelligence study services and health decision-making support services,etc.

Objective To investigate the morbidity and influencing infactors of plantar fasciitis in nurses. Methods The clinical nursing staff of 829 people were included in this series. 71 people of them conform to the standard of plantar fasciitis. According to the proportion of 1:1, 71 nurses without plantar fasciitis were included in the control group. The thickness and hardness index of the plantar fascia were detected by ultrasound elasticity. The survey contents including age, the average working hours every day (h), the average standing time in working day (h), body weight, body mass index (BMI), number of night shift, average walking time every day (min) and the average weekly running time (hour) were conducted. The single factor and multi-factor regression method were used to analyze the influence factors of the onset of plantar fascia. Results 71 cases of 829 nurses were with plantar fasciitis, and the incidence was 8.56%. The thickness in observation group was (3.86±0.53) mm, which was significantly smaller than (2.67±0.39) mm of the control group (P<0.05). Hardness index was 2.01±0.23, lower than 3.83±0.70 in control group (t=14.09, P<0.05). Single factor analysis results showed that the average standing time in working day, weight, BMI, average walking time every day, the average weekly running time were different between the two groups (P<0.05). The regression equation: the plantar fascia hardness index =-25.34 + 4.78 × the average standing time in working day + 3.45 × weight + 1.22 × BMI. The equation of the ability to explain the plantar fascia hardness index reached 72.2%, and had a good effectiveness. Conclusions Body weight and BMI, prolonged standing work may induce the nurse plantar fasciitis, and they are independent risk factors.

Objective To investigate the incidence and risk factors of concomitant atherosclerotic renal artery stenosis(ARAS)and coronary heart disease(CAD).Methods In the study period 247 patients with known or suspected CAD underwent combined cardiac catheterization and renal artery angiography.Results Incidence of ARAS was 20%(44/220)in CAD.Logistic regression analysis showed only age,degree of coronery artery stenosis,peripheral atherosclerosis disease were independently predictor of ARAS.Conclusions Renal artery angiography should be performed routinely to indentify ARAS,especially in the patients with mutil-vessel lesions,old age and peripheral atherosclerosis disease.

Objective To study the upper airway structure characteristics of children with obstructive sleep apnea hypopnea syndrome (OSAHS) using electronic pharyngorhinoscopy.Methods Seventy-eight children with OSAHS (34 cases mild group,44 cases moderate and severe group) and 52 children with chronic pharyngitis in the same period (control group) were selected.Each group was examined by electronic pharyngorhinoscopy.The adenoid-nasopharynx ratio (ANR),degree of palatopharyngeal anteroposterior diameter collapse,degree of palatopharyngeal right-and-left diameter collapse,degree of palatopharyngeal cavity collapse,degree of glossopharyngeal anteroposterior diameter collapse,degree of glossopharyngeal right-and-left diameter collapse,degree of glossopharyngeal cavity collapse were compared,and the relevance between thegn were analyzed.Results The ANR in mild group,moderately and severe group was significantly higher than that in control group (0.84 ± 0.21 and 0.69 ± 0.25 vs.0.34 ± 0.12),and the ANR in moderate and severe group was significantly higher than that in mild group.There were statistical differences (P ＜ 0.05).The degree of palatopharyngeal anteroposterior diameter collapse,degree of palatopharyngeal right-and-left diameter collapse,degree of palatopharyngeal cavity collapse,degree of glossopharyngeal anteroposterior diameter collapse,degree of glossopharyngeal right-and-left diameter collapse,degree of glossopharyngeal cavity collapse in moderately and severe group were significantly higher than those in control group and mild group (0.81 ± 0.25 vs.0.41 ± 0.17 and 0.54 ± 0.20,0.66 ± 0.23 vs.0.31 ±0.08 and 0.43 ±0.14,0.51 ±0.18 vs.0.14 ±0.04 and 0.23 ±0.11,0.55 ±0.21 vs.0.29 ±0.14 and 0.32 ± 0.13,0.69 ± 0.28 vs.0.42 ± 0.12 and 0.43 ± 0.11,0.33 ± 0.12 vs.0.10 ± 0.03 and 0.14 ± 0.05),and there were statistical differences (P ＜ 0.05).There were no statistical difference in the indexes between mild group and control group (P ＞ 0.05).In children with OSAHS,the ANR,degree of palatopharyngeal anteroposterior diameter collapse,degree of palatopharyngeal cavity collapse,degree of glossopharyngeal right-and-left diameter collapse,degree of palatopharyngeal cavity collapse were positively correlated with AHI (P ＜ 0.05),while there was no correlation between the degree of palatopharyngeal right-and-left diameter collapse,degree of glossopharyngeal anteroposterior diameter collapse and AHI (P ＞ 0.05).Conclusions Upper airway structural changes in children with OSAHS have certain correlation with the severity of sleep apnea.And there is clinical value by using electronic pharyngorhinoscopy in children with OSAHS to research airway morphology.

Animals ; Antibodies ; chemistry ; genetics ; Genetic Engineering ; Humans ; Immunoglobulins ; genetics ; Immunohistochemistry ; Protein Engineering

Objective: To identify the risk factors of fever in critically ill patients with acute stroke in a neurological intensive care unit (NICU). Methods: The frequency of risk factors of fever in 137 stroked patients during the first 7. days after admission were retrospectively studied. Fever was defined as a patient's axillary temperature ≥37.5°C in two separate determinations or >37.8°C in one determination. We categorized the fever into 4 kinds: Circled digit oneany fever, Circled digit twoexplained infectious fever, Circled digit threeexplained noninfectious fever and Circled digit fourunexplained fever based on Commichau's methods. Then logistic regression analysis was used to ascertain the risk factors of the 4 kinds of fever. Results: Any fever, explained infectious fever, unexplained fever and explained noninfectious fever occurred in 65%, 48.9%, 15.3% and 0.7% respectively. Multivariable logistic regression analysis demonstrated that patients older than 65 years, impaired consciousness, large area cerebral infarction/cerebral hemorrhage volume ≥30 ml, and deep vein catheterization used were involved into regression equation for any fever. In addition to these variables, length of NICU stay was also involved into regression equation for explained infectious fever. Brain midline shift, initial peripheral leucocytes count more than 12.0 × 10 9/L were involved into regression equation for unexplained fever. Conclusion: The risk factors differ in various kinds of fever in critically ill patients with acute stroke. Effective measures should be taken for different types of febrility.