Cytological characteristics of a case of adult T-cell leukemia were studied by detection of morphologic, cytochemical and immunological methods. The leukemic cells found in the peripheral blood belonged to mature T-lymphocytes and presented the changes of polymorphous nucleus which had diagnostic significance in association with clinical features and positive ATLA antibody. The mechanism of formation of cytological characteristics of adult T-cell leukemia is also discussed.

Inhibition of proliferation and induction of differentiation by 1, 25-dihydroxyvitamm D3 of human promyelocytic leukemic cell line (HL-60)are reported. At the concentration range of 109 to 106 mol/ L, 1, 25-dihydroxyvitamin D3 had effect in inhibiting cell proliferation with time-dependent and dose-dependent manner. Strong inhibition of colony cell growth (74.8?11.1%)was observed at 107 mol/L concentration. It was also found that HL-60 cells could be induced into monocytes by 1, 25-dihydroxyvitamin D3at the same concentrations,which were demonstrated by cytochemistry and specific antigen of cell surface and NBT reduction test. In addition, the relationship between proliferation and differentiation is discussed according to the result of flow cytometry.

HSP70 Heat-Shock Proteins ; analysis ; Hemorrhagic Fever with Renal Syndrome ; metabolism ; pathology ; Hepatitis Antigens ; analysis ; Hepatitis, Viral, Human ; immunology ; pathology ; Humans ; SARS Virus ; isolation & purification ; Severe Acute Respiratory Syndrome ; pathology ; virology

In order to probe into the mechanism of low-dose chemotherapeutic agents in the treatment of leukemia, we studied the effects of low concentrations (l-40ng/ml) of daunorubicin (DNR) on proliferation and differentiation of human promyelocytic leukemia cell line (HL-60). 1 ng/ml DNR could inhibit the proliferation of the cells, but no induction of differentiation was found. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation. Through the count of survival cells, [3H]TdR and [3H]UR incorporation, cloning efficiency, cell morphology and NBT reduction test, it is concluded that low-dose DNR could have inhibitory and cytotoxic effects on the cells, and its inducing differentiation was not important.

The effects of 1, 25 (OH)2D3 in combination with low-dose chemotherapeutant on the human promyelocytic leukemic cells (HL-60) were investigated. In 4-day-cultured cells, 1, 25 (OH)2D3 and low-dose harringtonine (HH) or daunorubicine (DNR) had synergic effect in inducing differentiation of HL-60 cells, and HH or DNR significantly potentiated the effect of 1, 25 (OH)2D3 in inhibiting the cell proliferation, which were demonstrated by NBT reduction test and [3H]-TdR incorporation, respectively. Cell cycle analysis by flow cytometery revealed that the cells in S phase decreased remarkably and most cells stopped at G0/G1 phase. The results provide a new way for the inducers in treatment of leukemia.

Objective To study the factors which affect the functional restoration of the elbow for adults with supracondylar or intercondylar fractures of the humerus treated with open reduction and internal fixation. Methods 22 patients with supracondylar or intercondylar fractures of the humerus were treated with open reduction and internal fixation from January, 1999 to December, 2004. They were followed up for an average of 30 months and complete clinical data were kept. The function of the elbow was evaluated with the Mayo elbow performance index. The relationship between the results and the factors —initial time of postoperative rehabilitation, fracture classification, operative approach and interval from injury to operation —were analyzed with cumulative logistic regression model of SAS software V8.2 (SAS institute, Cary, North Carolina). Results Of the 22 cases, 7 were excellent, 9 good, 5 fair and 1 poor. The excellent and good rates were 72.7%. And the odds ratio estimates of the 4 factors were 0.786, 0.299(B/A)0.221(C/A), 1.038, 0.197 respectively. Conclusions The initial time of postoperative rehabilitation, the fracture classification and the interval from injury to operation affect the functional restoration whereas the operative approach does not. Early operation, anatomical reduction, stable fixation and early rehabilitation will help improve the functional restoration.

Objective:The one-way communication between traditional LIS system and equipment has been unable to meet the needs of business development of medical laboratory in our hospital, we need to upgrade our equipment and to develop the bidirectional communication interface between LIS and equipment.Methods: Our hospital has invested a lot of money to upgrade previous One-way communication interface based on ASTM, the new instrument specifications conform to HL7 standard, and develop bidirectional transmission program based on HL7.Results: After the implementation of the bidirectional communication, testing equipment can automatically identify bar code to get the test request information, after the completion of the test instrument, the inspection unit can automatically send sample results to LIS.Conclusion: Bidirectional transmission improves the work efficiency, transmission based on HL7 is not only fast, but also conducive to the sharing of data between heterogeneous medical information systems, and it is a general tendency.

Objective To investigate the clinical advantages and safety profile of the combinational treatment of acute progressive cerebral infarction with aspirin,clopedogrel and atorvastatin.Methods 170 cases with acute progressive cerebral infarction were randomly divided into the treatment group and control group,85 patients in each group.Both two groups were initially treated with aspirin and atorvastatin.After excluding the possibility of hemorrhage, the treatment group additionally received clopedogrel,while the control group was treated with aspirin and atorvastatin. The difference in clinical efficacy was evaluated between before treatment and 7 days,14 days or 28 days after treatment by using the NIH Stroke Scale (NIHSS)and Barthel Index (BI).Results The NIHSS scores of the treatment group after 7d,14d and 28d were (12.52 ±3.25)points,(9.10 ±3.21)points and (6.87 ±2.85)points, which of the control group were (13.65 ±2.93)points,(10.73 ±3.41)points and (9.07 ±2.96)points respectively, the differences between the two groups were statistically significant(t =2.340,3.170,4.877,all P <0.05).The BI scores of the treatment group after 7d,14d and 28d were (35.26 ±11.18),(53.73 ±13.74)and (74.61 ±17.35), which of the control group were (31.98 ±9.12),(46.65 ±11.79 )and (63.87 ±15.73 )respectively,the differences between the two groups were statistically significant(t =2.131,3.752,4.456,all P <0.05).The overall effective rates of the treatment group after 7d,14d and 28d were 62.7%,79.5% and 94.0%,which of the control group were 51.2%,68.3% and 84.1% respectively.The differences of overall effective rates were statistically significant after 14d and 28d(χ2 =4.711,8.531,all P <0.05).Few reverse reactions were observed in both two groups.Conclusion Compared with the aspirin and atorvastatin therapy,combinational treatment of acute progressive cerebral infarction together with clopedogrel has a better efficacy,safety profile and significant promotion on neurological recovery.

Diabetes mellitus is a common disease of serious harmful to human health,but its pathogenesis is not entirely clear.Mitochondria are the important organelles to generate energy in eukaryocytes,and play a pivotal role in the regulation of reactive oxygen species generation,intracellular calcium homeostasis,and apoptosis signal transduction.The possible causes of mitochondrial dysfunction include oxidative stress,Ca2+ disturbances,reduction of mitochondrial biosynthesis,opening of mitochondrial permeability transition pore,mitochondrial DNA mutations,and etc..Many studies demonstrate that mitochondrial dysfunction is associated with β-cell dysfunction of type 1 diabetes mellitus and insulin resistance of type 2 diabetes mellitus.Moreover,mitochondrial dysfunction plays important roles in the development of diabetic cardiomyopathy.This present article reviewed the current status of studies on the relationship of mitochondrial dysfunction and diabetes mellitus and diabetic cardiomyopathy.It is very important to understand and study mitochondrial dysfunction and its important roles in diabetes mellitus and diabetic cardiomyopathy in order to clarify the pathogenesis of diabetes mellitus and explore new approaches of prevention and treatment for diabetes mellitus.