Objective To study the effects of the RNA secondary structure on the RNA interference efficacy and to optimize the RNAi target sequence selection.Methods N-Ras mRNA in K562 human chronic erythro-leukemia cell line was selected as the target for RNAi experiments.Four siRNAs were designed aiming at secondary structure region and none-secondary structure region separately.The N-Ras expression change and the cell growth was tested by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR),light microscopy,MTT,and flow cytometry.Results Knock-down efficiency was recorded in all of the 4 experiment groups,but the degree varied a lot.The siRNAs targeting none-secondary structure region presented higher silencing efficiency than those targeting secondary structure region.Conclusion The secondary structure of mRNA closely relate to the RNA interference efficiency,which should be considered in the processing of the target sequence selection.

Objective To investigate the significance of peroxisome proliferator-activated receptor (PPAR)γexpression in the lung tissue of rats with chronic hypoxic pulmonary hypertension (HPAH). Methods Forty male Sprague-Dawley rats were randomly divided into four groups (n=10 for each group):normal control group (NC), hypoxia control group-one-week (HC-1w), hypoxia control group-two-week (HC-2w) and hypoxia control group-three-week (HC-3w). Normal control group was raised under normal oxygen condition in ventilated animal cage for three weeks. The other HC groups were placed in a low oxygen chamber (O2 concentration of 10%) from 9:00 AM-5:00 PM (8 h/d) everyday by one week, two weeks and three weeks. The mean pulmonary arterial pressure (mPAP), right ventricular systolic pressure (RVSP) were detected. The index of right ventricular hypertrophy RV/(LV+S) was measured by dissecting rat heart. The morphological changes of the small pul-monary arteries were observed by HE staining, and the percentage of vascular wall thickness (WT%) was calculated. The ex-pression level of PPARγprotein was detected by Westren blot assay. Results The mPAP, RVSP and RV/(LV+S) were sig-nificantly higher in HC groups than those of NC group (P<0.05). The morphology of pulmonary arteries showed vessel wall thickening and vessel lumina stenosis in HC groups compared with that of NC group. The PPARγexpression in lung tissue was significantly lower in HC groups than that of NC group, and the downward trend was more obvious with the extension of time. Conclusion PPARγplays an important role in the occurrence and development of chronic hypoxic pulmonary hyper-tension.

Objective To study the comparison of the therapeutic effect of Ultrasound guided and freehand botulinum toxin type of the treatment in children with cerebral palsy.Methods From March 2014 to September 2016,98 cases of children with cerebral palsy in our hospital by injection of botulinum toxin were treated.According to the way of injection,the children were divided into ultrasound group and free group(n =49).They were respectively gave botulinum toxin injection by guided Ultrasound guided and freehand ultrasound injection of hotulinum toxin injection.The amount of two groups of children was 3U/kg.Children muscle spasm degree of before and after treatment was assessed by modified Ashworth spasm scale.And exercise capacity of children before and after treatment was assessed by the gross motor function rating scale.The follow-up time was 3 months,the clinical curative effect of two groups of children was observed.Results The total efficiency of ultrasound guided group (98.0％) was significantly higher than the free group (79.6％),with statistically significant (P ＜ 0.05).Both groups before and after treatment in children with Ashworth score and GMFM score were significantly improved,with statistical significance (P ＜ 0.05).Ashworth score and GMFM scores of ultrasound guided group were significantly better than the free group,with statistically significant (P ＜ 0.05).Conclusion Botulinum toxin injection in the treatment of cerebral palsy children can effectively solve the spastic problem.Treatment of children with cerebral palsy by injecting botulinum toxin with ultrasound guided injection of botulinurn toxin is better than manual injection of botulinum toxin.

The mechanism of invasion and metastasis of colorectal carcinoma was associated with many aspects, including correlative genes such as k-ras,c-met,nm23,c-SRC,HES-6 and P107; immunoreaction; angiogenesis; correlative molecules interacting with extracellular matrix,nitric oxide. The paper summarized the current development in this field.

OBJECTIVE: To observe the efficacy of rhinoplasty with autogenous nasal septal cartilage for saddle nose caused by nasal bone and nasal septum fracture after trauma. METHOD: During the treatment process of 30 cases of nasal trauma, simple nasal bone fracture reposition and nasal septum correction was done, and rhinoplasty was done using the nasal septal cartilage. All the patients were followed up to evaluate subsequent effect. RESULT: All the patients were satisfied with the result of one stage repairment. After following-up for 3-42 months, 28 cases (93.3%) were of long-term satisfaction. CONCLUSION Rhinoplasty with autogenous nasal septal cartilage for saddle nose was an effective method to repair the nasal trauma.
Adolescent ; Adult ; Female ; Humans ; Male ; Middle Aged ; Nasal Cartilages ; transplantation ; Nasal Septum ; surgery ; Nose Deformities, Acquired ; surgery ; Rhinoplasty ; methods ; Transplantation, Autologous ; Treatment Outcome ; Young Adult

Objective To observe the clinical efficacy of RuPi decoction in the treatment of breast hyperplasia. Methods 100 cases with breast hyperplasia were randomly recruited into a treatment group, which was treated with the RuPi decoction, and a control group, which was treated with RuNing tablet. Three months constituted one course of treatment for both groups. Observe the clinic curative effects, and improvement of major symptoms and signs. Results The total effective rate of the treatment group and control group was 96% and 84% respectively, showing significant difference between the tow groups(X2=5.01, P<0.05). Conclusion Modified RuPi decoction was effective in the treatment of Breast Hyperplasia.

This paper analyzed the Chinese Medical Science and Technology Award gained by military health system during 2001- 2009,which showed the importance and necessity of social awards to the military health system.We also discussed the problems including insufficient importance attached by the military health system,inadequate enthusiasm and motivation of individual researchers,uneven distribution of the scientific level,as well as the number of awards and subjects.

Clinical Trials as Topic ; Humans ; Leukemia ; drug therapy

As a major ingredient of high medical college education,experiment practice plays an important role for medical students in integrating the medical theory with practice,training their capability of analyzing and thinking to definitively solute the problems in their study.Microbiology experiment is characterized with strong comprehensiveness and manipulation,which requests the students both aseptic operation and consciousness of bio-safety.To promote the student's comprehensive diathesis and to improve the experiment teaching quality,several common problems of students in medical microbiology experiment practice were analyzed and discussed in this article.

ObjectiveTo investigate the level of plasma stromal cell-derived factor-1 (SDF-1) in essential hypertension and the effect of angiotensin-converting enzyme inhibitor Enalapril on it. MethodsThe levels of plasma SDF-1 in 34 patients with hypertension and 28 normal healthy people were detected with ELISA. Hypertension patients were detected again after 2-week Enalapril treatment. ResultsThe level of SDF-1 in hypertension patients was （4.211±1.132） mmol/L, higher than control (3.568±0.936) mmol/L （P<0.05）, and became （4.950±1.050） mmol/L after treatment, higher than pre-treatment （P<0.05）. ConclusionThe level of SDF-1 increases in patients with hypertension, and Enalapril can further increase the level.