Objective To investigate the application value of warming humidification oxygen therapy in patients after tracheotomy.Methods Forty-one patients after tracheotomy were collected consecutively for this study.All patients were randomly assigned into the treatment group (21 cases)and the control group (20 cases).The treatment group adopted warming humidification oxygen therapy,and the control group adopted common airway moistening method.Moist effect judgment,viscosity of sputum,phlegm scab formation,respiratory irritation symptoms,airway mucosal bleeding,air way resistance,lung infection cases,average days in the ICU were investigated.Results There were statistically significant differences in moist effect judgment,viscosity of sputum,phlegm scab formation,respiratory irritation symptoms,airway mucosal bleeding,air way resistance,lung infection cases,average days in the ICU between the two groups.Conclusions Warming humidification oxygen therapy is more suitable to patients with tracheotomy than common airway moistening method.

The establishment of new teaching model in the open teaching environment has promised dramatic changes in the way we teach and learn in medical English.Through the combination of the character of medical English teaching and our experience of On-line Medical English Teaching,we introduce a cooperative model in Web-based course design for enhancing traditional face-to-face classroom teaching for further improvement in the process of reform.

Background Studies have showed an increasing prevalence of glaucoma in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS).However,this view remains controversial.Objective This Meta analysis was to assess whether there is an association between glaucoma and OSAHS.Methods A systematic search in PubMed database was carried out with the subject headingssleeping apnea andglaucoma.The literature type was limited to cases-controlled studies and prospective cohort studies with the publishing duration from January 1,1982 to January 30,2015 in English.The risk of glaucoma among OSAHS patients was analyzed,and Review Manager 5.2 was applicated for Meta analysis.The pooled odds ratio (OR) and 95％ confidence interval (CI) was calculated to assess the strength of the association.Results Twelve independent retrospective casescontrolled studies were included in this review,including 11 592 subjects in the OSAHS group and 25 931 subjects in the control group.The study quality was scored 6-8 by Newcastle-Ottawa Scale which was acceptable.The random effects model was accepted because of the heterogeneity among the studies (x2=34.20,P＜0.05,I2 =68％).The prevalence of glaucoma was higher in the OSAHS group than that in the control group (OR =1.87,95％ CI:1.21-2.90,Z=2.82,P＜0.05).The subgroup analysis of OSAHS showed that the OR (95％ CI) of mild,moderate and severe OSAHS groups versus control group was 3.61 (0.56-23.43),4.17 (0.47-36.91) and 6.95 (1.14-42.26),respectively.Sensitivity analysis confirmed that the OR value fluctuated in 1.74-2.16 and closed to 1.87.Funnel graphy exhibited a asymmetry appearance among the literatures,which suggested a possible publication bias.Conclusions OSAHS is one of the risk factors of glaucoma.The serious OSAHS is associated with an increased risk of glaucoma.

Objective To comprehend the characters of sensory integration of children with brain injury and identify the effect of sensory integration training.Methods 127 children with brain injury were determined by the check form of sensory integration, Wechsler Preschool and Primary Scale of Intelligence (WPPSI), Wechsler Intelligence Scale for Children-China Recension (WISC-CR) and comprehensive function assessment for disabled children. These children were divided into the trial group (n=92) and control group (n=35). All children of two groups received rehabilitation training, but children of the trial group also received sensory integration training. The Results of them were compared before and after rehabilitation.Results The scores of the check form of sensory integration of the children with cerebral palsy were significantly higher than that of children with mental retardation (P<0.01). After rehabilitation training, the raising scores of comprehensive function assessment for disabled children of the trial group were significantly higher than the control group (P<0.01).Conclusion The ability of sensory integration are abnormal in children with brain injury. The sensory integration training can accelerate the comprehensive function of children with brain injury effectively.

Objective To establish the fingerprint of Gentiana straminea from Qinghai Province by HPLC. Methods By orthogonal design and related methods based on information theories and chemometrics to optimize the proper HPLC conditions so that the fingerprint spectra of ten groups of different samples have been formed. Results The experimental results are the fact that the common fingerprint peaks, precision, and repeatability in the fingerprint totally meet the relative regulations required in Technical Requirements of the Fingerprint Research in Injection of Chinese Materia Medica (Tentatine Standard). Conclusion The chromatographic fingerprint can be used to identify and evaluate the quality of G. straminea.

Objective To investigate the killing effect of allitride on fungus inside Candida albicans biofilm (BF) .Methods The polyascchride components of BF was labeled by FITC-conA .The fluorescence microscopic was adopted to observe the effect of al-litride on the BF forming process of Candida albicans in morphology .The specimens were divided into 3 groups ,the control group (allicin concentration of 0) ,0 .25 mg/mL allicin group ,0 .5 mg/mL allicin group ,1 .0 mg/mL allicin group ,2 .0 mg/mL allicin group .The XTT reduction assay was performed to quantitatively detect the killing effect of allitride on fungus inside Candida albi-cans BF .Results After the treatment by different concentrations of allitride ,the BF structure was observed by fluorescence micros-copy .The fluorescence intensity of high concentration group was weakened compared with the low concentration group and the con-trol group .The ODA450 values of the XTT reduction assay after acting on Candida albicans BF for 5 h by different concentrations of allitride (0 .5 ,1 .0 ,2 .0 mg/mL) were 1 .87 ± 0 .31 ,1 .64 ± 0 .25 and 1 .30 ± 0 .29 ,respectively ,and the OD450 value of the control group was 2 .11 ± 0 .26 ,there was statistically significant difference between the allitride groups and control group (P<0 .05);after acting on Candida albicans BF by 0 .25 mg/mL allitride ,the OD450 value was 1 .93 ± 0 .31 ,the difference had no statistical signifi-cance compared with the control group(P>0 .05) .Conclusion Allitride can kill the fungus inside BF of Candida albicans and has the inhibiting effect on biofilm .Moreover the inhibiting effect on biofilm is increased with its concentration increase within certain concentration range .

We designed an elective course of its professional characteristic——online reading for undergraduates majoring in medicine,in order to probe the mode of reform in education of English.

BACKGROUND:Repair of large-area burns and severe post-traumatic skin defects has always been urgent clinical breakthrough technology bottleneck. With the development of tissue engineering, epidermal stem cel s are increasingly being used in tissue engineering, cel replacement therapy and genetic engineering. Therefore, the isolation and identification of epidermal stem cel s is becoming the research focus of concern.
OBJECTIVE:To review the research progress in biological markers of epidermal stem cel s.
METHODS:The Chinese Biomedical Literature database, CNKI database, China Academic Journals Ful-text database, PubMed database and EMbase database were retrieved for articles about specific markers of epidermal stem cel s using the keywords of“epidermal stem cel s, integrin, keratin, P63, CD71, telomerase, ACE, cx43, hoechst”in Chinese and English. Older theoretical perspectives and repetitive research were excluded.
RESULTS AND CONCLUSION:Final y, only 40 articles were included in result analysis. Epidermal stem cel s bring a new source for skin tissue repair. Epidermal stem cel s distribute in the fol icle eminence and basal layer of the epidermis. About 4%cel s in the basal layer, however, are stem cel s. Therefore, it is critical to correctly isolate, culture and identify skin stem cel s. As a reason, specific markers of epidermal stem cel s become a hotspot. Currently, a great progress in the biological markers of epidermal stem cel s has been made, but there is stil no absolute and proven marker for epidermal stem cel s. Most studies are focusing on integrin, keratin, P63, CD71, connexin 43, and telomerase. In addition, hoechst, CD90, CD98, CD200 have been reported recently. Each marker has its own shortcomings, and there are stil many problems that need to be solved.

Objective To investigate the relationship between the expression level of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in placenta tissue and placenta increta.Methods Thirty singleton pregnant women who received antenatal care and underwent cesarean section in Fujian Maternity and Child Health Hospital between November 2013 and August 2014,were enrolled in this study.They were divided into placenta previa group,placenta increta group and control group,with ten patients in each group.Placenta tissue was collected from each patient.Expressions of VEGF and its mRNA were detected by immunohistochemistry,Western blot,and real-time polymerase chain reaction.MVD in placenta tissue was measured by immunohistochemistry.Rank sum test,t test,Kruskal Wallis test,one-way ANOVA and Spearman correlation test were used for statistical analysis.Results (1) Gestational age at admission and delivery in placenta previa and placenta increta groups was lower than in control group (all P＜0.05).Compared with control group,the placenta previa and placenta increta groups had more blood loss,and longer operating duration and hospital stay (all P＜0.05).(2) The expression levels of VEGF and its mRNA in placenta increta and placenta previa groups were higher than in control group (VEGF:0.691±0.032,0.695 ± 0.027 and 0.518±0.025,respectively,F=373.401;VEGF mRNA:1.667±0.661,1.832±0.678 and 0.767±0.269,respectively,F=27.399;both P＜0.05).But there was no significant difference between placenta previa and increta groups.(3) There was no significant difference of VEGF expression in increta location,border site and normal site in placenta increta group,but its mRNA was decreasing (2.519± 0.116,1.482 ± 0.232 and 1.000± 0.000,respectively,F=240.827,P＜0.05).(4) Expression level of VEGF at the attachment of umbilical cord,upper and lower margin of placenta in placenta previa group was higher than in control group (0.702 ± 0.026 vs 0.528± 0.020,t=12.302;0.698 ± 0.026 vs 0.519±0.035,t=12.715;and 0.685±0.029 vs 0.509±0.010,respectively,t=17.891;all P＜0.05).Expression of VEGF mRNA in placenta previa group was higher than in control group (2.080± 0.539 vs 1.024±0.272,t=8.093;1.587±0.757 vs 0.546±0.083,t=2.401;1.828±0.704 vs 0.731 ±0.157,t=4.259;all P＜0.05).(5) MVD in placenta increta group and placenta previa group was higher than in control group (171.2± 14.7,155.7± 14.6 vs 147.8±12.3,respectively,F=7.277,P＜0.05).(6) Expression level of VEGF in placenta increta group and control group was positively associated with MVD (r=0.825,P＜0.05).Conclusions There may be some common mechanisms in the occurrence of placenta previa and placenta increta.Overexpression of VEGF in placenta and abnormal formation of villous vessels may be important factors in the pathogenesis of placenta increta.

OBJECTIVE:To establish the HPLC method for content determination of Azithromycin suspension.METHODS:The determination was performed on Venusil XBP-C18(L) (150 mm?4.6 mm,5?m) column with mobile phase consisted of 0.05 mol?L-1 dipotassium phosphate (pH value adjusted to 8.2 by 20% phosphoric acid)-acetonitrile (45:55) at flow rate of 1.2 mL?min-1.Injection volume was 20 ?L and UV detection wavelength was set at 210 nm.RESULTS:The good resolution between peaks of azithromycin and that of adjacent purity was more than 1.5.The linear range of azithromycin was 0.01～5 mg?mL-1 (r= 0.999 9) with an average recovery of 98.83%(RSD=0.54%,n=6).The precision of intra-day,inter-day and with-day were all lower than 2.5%.CONCLUSION:The method is specific and accurate for the content determination of Azithromycin suspension.