Objective To understand the learning needs of nursing students for nursing English in senior high vocational school.Methods A survey was carried out among 982 three-year nursing students in grade 2007-2009 in Cangzhou medical college by questionnaire.The data collected were analyzed using descriptive statistics with SPSS 17.0.Results Most students (93.0％) believed that nursing English was important and had a strong desire for most English skills,especially speaking and listening ability.They (90.7％) expected nursing teachers to take charge of or participate in teaching work and some students (30.6％) were dissatisfied with teaching material.They depended on teachers' instruction and preferred learning in real situations.Conclusions Nursing students in senior high vocational school have strong needs for nursing English,but there is a gap between current teaching situation and learning needs,which the course designers and teachers should take into full consideration in order to make the teaching more effective.

Objective To recommend the appropriate retrieval tools for scientific researchers and improve their re-trieval efficiency by comparatively analyzing the retrieval results from PubMed and Embase .com.Methods Eight retrieval terms from different subjects were used to retrieve PubMed and Embase .com by subject headings and basic retrieval methods than can automatically transfer their function .Results Six different results were retrieved from PubMed and Embase.com with 8 retrieval terms.Conclusion The recall ratio is higher in Embase.com than in PubMed when they are retrieved by subject headings while the accuracy ratio is higher in PubMed than in Embase . com when they are retrieved by basic retrieval methods than can automatically transfer their function although error transformation occurs in both PubMed and Embase.com.

Reference service is one of the key tasks in library.The current situation of reference service in library and its problems were analyzed from the need of readers, and the problems to which attention should be paid were pointed out from its convenience and time-effectiveness .

With the development of clinical pharmacy in hospital,the demand for pharmacy intravenous admixture services (PIVAS) has been gradually increased.By being built inside the pharmacies,PIVAS of this hospital has created a full range of integrated pharmacy service platform.With independent research and development of information management system,innovative design of the modules and equipments based on the concept of internet of things,and integration of artificial and computerized checking,PIVAS of this hospital has formed a system of prevention and control chain,it has been deepening the connotation of clinical pharmacy services,and providing higher quality pharmacy services to clinical drug using.

Humans ; Integrative Medicine ; methods ; Medicine, Chinese Traditional ; methods ; Neoplasms ; prevention & control ; therapy ; Quality Control ; Research Design

Viral myocarditis can cause severe myocardial injury and even acute heart failure, then evolve into dilated cardiomyopathy, known as inflammatory cardiomyopathy. Immune reaction plays a key role in the transformation from viral myocarditis into dilated cardiomyopathy, including virus RNA continuous replication,immune-mediated injury, cellular apoptosis, other cellular immune response , myocardial antibodies and other humoral immune response. In the clinical treatment, besides anti-heart failure and cardiac nutrition, the use of ribavirin, α1-receptor blockers, β-receptor blockers, calcium channel blockers, angiotensin converting enzyme inhibitors and spironolactone should be emphasized.

Objective:To investigate the infloence of RNA interference targeting P13K p85α on the invasion and metastasis of colorectal cancer cells. Methods:Foor shRNA vectors and one negative control vector were designed and stably transfected into LoVo cells. Western blotting analysis was used to screen for the groop which had the highest inhibitory rate; the cells were sobjected to woond closore assay and transwell assay to determine the migration of LoVo cells after stable transfection. Results: Western blotting analysis showed that cells transfected with shRNA/324 vector had the strongest inhibition of P13K p85α protein, with the inhibition rate being 77% , and cells in this groop were osed for woond closore assay and transwell assay. Wound cloaore assay showed that the healing ability of P13K p85α-depleted cells was significantly lower than that of the control cells (P<0.05). Transwell assay showed that the cells passing the polycsrhonate membranes was significantly less in P13K p85α-knockdown cells than in the control cells (P<0.05). Conclusion:Depletion of P13K p85α protein can obvioosly inhibit the migration of LoVo cells. P13K p85α may be a new therapeotic target for treatment of colorectal cancer metastasis.

OBJECTIVE: To explore the method for the objective evaluation of single limb function after in- jury in forensic medical practice. METHODS: The score of activities of daily living (ADL) were graded for a single limb function after injury from 47 cases. All cases were simultaneously evaluated using the different methods including Fugl-Meyer motor function assessment (FMA), weighting, look-up table (LUT). The correlation were compared between ADL and the other three methods. RESULTS: Injured part and the score using the three methods were correlated with ADL score (P < 0.05). The correlation coeffi- cient (|r| value) showed highest using LUT method, and lowest using FMA method. CONCLUSION The loss function of limb is affected by the injuried parts. The methods of FMA, weighting and LUT show a good accuracy for evaluating the limb function after injury and the correlation presents higher using LUT method.
Activities of Daily Living ; Disability Evaluation ; Extremities/injuries* ; Forensic Medicine/methods* ; Humans

Objective: To assess the synergistic anti-inflammatory effect of sodium ferulate (SF) and kushenin, i.e. oxymatrine (OMT). Methods: The anti-inflammatory effect of the combination of SF and OMT was evaluated on xylene-induced murine ear edema model, carrageenin-induced rat paw edema model, and rat tampon-induced granuloma model in vivo. In vitro, the effect of SF and OMT on the nitric oxide (NO) production induced by LPS was observed on RAW264.7 cells. The content of NO in the supernatant was detected with Griess reagent. Results: When SF was used in combination with OMT, the edema induced by xylene and carrageenin was markedly inhibited, the weight of granuloma caused by tampon was also reduced in the high dose group, and the inhibition rate was more considerable by combined treatment than those by SF or OMT alone. In vitro, the NO production induced by LPS was depressed by combined treatment, and a synergistic anti-inflammatory effect was observed. Conclusion: The present study demonstrate that the combination of SF and OMT could exert conspicuous synergistic anti-inflammatory effect, and the mechanism may be related to the inhibited effect of NO production in inflammatory factors induced by LPS.

Objective To investigate The effect of TAM(2.5 uM)in the chemotherapy of HCC and its mechanism. Methods An adriamycin-resistant human HCC cell subline(Hep-3B/ADM)was established,through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Modulation of ADM-induced apop- tosis of Hep-3B/ADM cells was assessed by Flow cytometry(FCM).PKC activity was determined by applying compet- itive protein binding method.Results TAM(2.5uM )decreased the IC50 of ADM from 1.64?g/mL to 0.23?g/mL in the Hep-3B/ADM cells.TAM exhibited a 2-fold potentiation on apoptosis caused by ADM in Hep-3B/ADM cells.With TAM,PKC activity was lower than that in control groups from 30 seconds to 24 hours.But this effect of TAM could be reversed by the co-treatment of the cells with TPA.Conclusion TAM(2.5 uM)significantly en- hanced ADM-induced cytotoxicity.The mechanism is that TAM potentiates the apoptosis induced by ADM through pro- tein kinase C inhibition.