Objective To compare the operation effect of endoscopic versus open component separation on abdominal wall reconstruction.Methods From January 2012 to December 2013,19 patients (ECST 8 and CST 11) with abdominal wall defects were repaired with biomaterials mesh.This study analyzed retrospectively the clinical information between two groups.Results No hernia recurrences were occurred during a mean follow-up of (14.96± 8.89) months.There was no significant difference in EBL,operation time,length of stay.The rate of recurrence wound complications in CST group is higher than the ECST group,but is not significant difference.Conclusions Endoscopic or open component separation with biomaterials mesh could effectively repair the abdominal wall defect and ECST definitely showed the advantage in reducing the incisional complication.But the large sample,long-term clinical follow-up should be confirmed.

Objective To observe the effect of heat-producing needling (Shao Shan Huo) and cool-inducing needling (Tou Tian Liang) on the gait cycle after operation for gluteus contracture. Method Sixty-six patients after operation for gluteus contracture were randomized into a treatment group and a control group, 33 in each group. The control group was intervened by rehabilitation, while the treatment group was by acupuncture in addition to the rehabilitation training. The topical points Zhibian (BL 54), Huantiao (GB 30), and Juliao (GB 29) were treated with reducing cool-inducing needling technique; Biguan (ST 31), Futu (ST 32), Zusanli (ST 36), Fenglong (ST 40), Xuehai (SP 10), Diji (SP 8), and Yanglingquan (GB 34) were treated with reinforcing heat-producing needling technique. The motor time parameters were observed before and after intervention.Result After intervention, the supporting phase of the affected side, supporting phase of the healthy side, gait cycle, swinging phase of the affected side, swinging phase of the healthy side, and bilateral supporting phase of the treatment group were significantly different from that of the control group (P<0.05).Conclusion Heat-producing and cool-inducing needling techniques can effectively shorten the gait cycle after operation for gluteus contracture, and can improve the walk function.

Objective To determine quantity of type Ⅰ collagen and mRNA expression of decorin and transforming growth factor (TGF)- β_1 in the upper portion of the anterior vagina in women with pelvic organ prolapse (POP). Methods Transvaginat biopsies were obtained from the anterior vaginal wall in 27 POP(POP group) and 21 non-poP(control group). The concentration of type Ⅰ collagen was determined by ELISA technique. RT-qPCR was used to verify the mRNA level of decorin and TGF- β_1. Results The mean concentration of type Ⅰ collagen in the premenopausal women in POP group [(47.94 ± 6.82) mg/g total protein (TP)]was significantly lower than that in control group [(62.33±6.57) mg/g TP],in the postmenopausal women in POP group, it was (41.82±5.96) mg/g TP, significantly lower than that in control group[(61.21 ± 4.84)mg/g TP](P< 0.01). The mRNA expression of decorin was 1.61 ± 0.10 in POP group, significantly lower than that in control group 2.77±0.60 ,while that of TGF-β_1 was higher in POP greup10.86 ± 0.23 than that in control group 9.87 ± 0.92 (P < 0.05). In the postmenopausal women in two groups, there were no significant difference in the mRNA expression of decorin and TGF-β_1 (P > 0.05). Conclusions A decrease in quantity of type Ⅰ coUagen in the connective tissue of anterior vagina may compromise the tensile strength and an increase susceptibihty to prolapse. Deeorin might be involved in the pathogenesis in premenopausal women with POP. The pathogenesis of POP is muhifactorial.

Objective: To investigate the expression of apoptosis-related proteins Fas and caspase-3 in bladder transitional cell carcinoma (BTCC) and their clinical significance. Methods: The bladder tissues were isolated from 45 BTCC patients and 10 healthy controls and embedded in paraffin. The expression of Fas and caspase-3 were detected by streptavidin peroxidase inummohistochemical method. The results were analyzed associated with clinical records. Results: The positive rate of Fas expression was 46.7% (21/45) in TCC and 100% in the 10 normal bladder tissues. The positive rate of caspase-3 expression was 37. 8% (17/45) in BTCC and 90% (9/10) in normal control group. The difference was significant (P < 0.05). The expression of Fas was significantly related with the histological grades, the initiation of BTCC, either primary or recurrent, and the tumor number and but not associated with clinicopathological stages. While the expression of caspase-3 correlated with the initiation of BTCC, either primary or recurrent; but it did not correlate with the histological grades, tumor numbers, and the pathological stages of BTCC. There was a positive correlation between the expression of Fas and caspase-3 in BTCC. Conclusion: The decrease in the selective expression of Fas is closely associated with the differentiation degree of BTCC. The expression of caspase-3 is down-regulated in BTCC. Expression of Fas and caspase-3 are important to indicate the prognosis of BTCC.

Objective: To study the mechanism that erythrocytic stages Plasmodium berghei chloroquine resistant strain (RC strain) were eliminated in their hosts. Methods: Interactions of leukocytes with the parasites in mice livers infected with the RC strain or chloroquine sensitive strain (N strain) of P. berghei were studied by electron transmission microscopy. Results: None of leukocytes proliferated and infiltrated in mice livers infected with the N strain. Whereas in mice livers infected with the RC strain, proliferation and infiltration of monocyte macrophages, lymphocytes and nuetrophils occurred within portal areas and hepatic sinusoids with sequestration of numerous parasitized erythrocytes. The activated monocyte macrophages adhered to the parasitized red blood cells with their surface membranes. A lot of the parasites became crisis form within the parasitized erythrocytes which directly contacted with the phagocytes or not. Phagocytosis of free merozoites by macrophages was rarely revealed, but of the whole parasitized erythrocytes was not found. Conclusion: The crisis form of the parasites induced by the activated monocyte macrophages, rather than the direct phagocytolysis, is mainly responsible for the elimination of the P. berghei RC strain in their hosts. [

OBJECTIVE:To provide reference for rational use of antiemetics in cancer patients. METHODS:Two thousand six hundred and sixteen pieces of medical orders of patients receiving antiemetics were collected from oncology department of our hospital via EMRS during Oct. 2015-Jun. 2016. RESULTS:Among 2 616 cases surveyed,1 301 cases conformed to inclusion and exclusion criteria,of which irrational use of drugs were found in 595 cases,760 times in total. Main manifestations were unsuitable drug selection(33.82%),irrational usage and dosage(25.26%),unsuitable drug combination(34.08%)and non-standard medication course(6.84%). The incidence of nausea in female was higher than male,with statistical sighificance(P=0.003),but gender had no significant effect on the incidence of nausea(P>0.05). Age had no significant effect on both nausea and vomiting(P>0.05). The control rate of nausea/vomiting in rational drug use group was higher than irrational drug use group. Average cost of antiemetic treatment of different emetic risk chemotherapy drugs in rational drug use group were all lower than irrational drug use group, indicating therapeutic efficacy could be guaranteed and economic burden was reduced in rational drug use group. CONCLUSIONS:The antiemetic plan should be selected rationally according to emetic risk degree of chemotherapy drugs. Patients receiving combined chemotherapy should select antiemetic plan according to highest emetic risk degree of chemotherapy drugs,and strictly master usage, dosage and duration. At the same time,treatment cost should be considered when selecting antiemetic plan to guarantee the safe, effective,economical and rational use of drugs.

OBJECTIVE:To put forward game strategy of drug safety regulation,and to provide reference for decision-making by drug regulation department. METHODS:Based on evolutionary game theory,evolutionary game model of drug safety regula-tion was established,and the stability of evolution during drug safety regulation was analyzed. RESULTS & CONCLUSIONS:There were four possible evolutionary game results of drug safety regulation,including to provide qualified drugs,regulation;to provide unqualified drugs,regulation;to provide qualified drugs,no regulation;to provide unqualified drugs,no regulation. The evolution direction mainly depends on the parameter values involved in the payoff matrix of drug safety regulation. The stable state of drug safety regulation is related to regulation strength,regulation cost,enterprise safety input,probability of accident,acci-dent-related treatment cost,et al. Key strategies to improve drug safety regulation include increasing punishment,improving moni-toring technology,strengthening coordination mechanism,promoting social cohabitation and improving industry self-control.

Objective To compare surgical and interventional treatment in congenital ventricular septal defect in membrane position.Methods Among ventricular septal defect (VSD)in 384 cases in children,202 cases had surgical repair thoracotomy (tho-racotomy group),and 182 cases had interventional treatment (intervention group).The comparison items included operative time, success rate,intraoperative and postoperative blood transfusion,complication rate,postoperative recovery for several days after ICU monitoring time,CPB time,pericardial drainage time,duration of mechanical ventilation.Results Children in the intervention group did not need blood transfusions,ICU care,pericardial drainage,mechanical ventilation.In the thoracotomy group,the blood transfu-sion was (372.45±200.88)mL,postoperative ICU monitoring time was (3.21 ±2.1 7)days,CPB time was (71.09 ±34.92)mi-nutes,pericardial drainage time was (3.52 ± 1.22)days,mechanical ventilation time was (67.09 ±43.83)minutes.The operative time and postoperative recovery time in the intervention group was significantly shorter than thoracotomy group (P <0.05 ).The incidence of postoperative complications in the intervention group was significantly lower than thoracotomy group (P <0.05).Con-clusion Under the circumstances of the same indication,the interventional treatment is more beneficial to physical and mental health of children.

It is still a challenge for surgeons to deal with the complex abdominal wall defect.The purpose of surgical treatment is not only to restore the integrity of the abdominal wall, but also to maintain the function of the abdominal wall.Accurate classification and partition of the abdominal wall defects before operation are the base of surgical procedure for complex abdominal wall defects.Reinforcement of the abdominal wall defects with mesh, component separation technique and tissue flap technique are important methods to achieve functional repair of the abdominal wall defects.

To insight into genetic differences between chloroquine-resistant line (RC) and chloroquine-sensitive strain (N) of Plasmodium berghei. MethodsAfter continous cbloroquine-pressure upon RC line at higher dosage (50mg/kg. d) ,total RNAs from the RC line and the N strain of P. berghei were isolated for simplified differential display reverse transcript polymerase chain reaction (sDDRT-PCR ). The generated differential fragments had been repetitively rescued and identified by PCRs before one pair of suspected differential fragments (N25 and R25 )were cloned and sequenced. Then, their sequences were analyzed through PC-gene program and BLAST search. ResultsThough the identity of two nucleotide sequences of N252 and R251 ,cloned separately from the N25 and R25 fragments, were up to 99.8％ ,their predicted amino acid secondary structures were quite different due to multiple mutations. Compared with the published sequences in GeneBank,EMBL,DDBJ and PDB database ,no similar gene was found ,using BLAST search. However their partial nucleotide sequences (62nt from query 128nt to189nt bore highly homology to part sequence(from 1053nt to1114nt)of rattus norvegicus mRNA for phospholipase B,up to 93.5％ in N251 and 91.9％ in N252 respectively. ConclusionIt is feasible to isolate chloroquine-resistant related genes by using simplified DDRT-PCR combined repetitively rescuing and PCR identifying the interest differential DNAs together with sequence analyses.