Humans ; Practice Guidelines as Topic ; Research Design

Meta-Analysis as Topic

Due to the serious shortage of donor cornea materials and the donor limit, clinical popularization of penetrating keratoplasty is severely restricted. lt is a hot spot of current research that applying tissue engineering in vitro to culture corneal endothelial cells ( CEC ) with high density, regular hexagonal shape and healthy endothelial function. ln this article, we reviewed the latest progress in the study of source of CEC seeder cells, selection of cultivating carries, type of CEC transplantation and immune mechanism that summarized the current research problems and made a prospect to the future.

Animals ; Evidence-Based Medicine ; Health Knowledge, Attitudes, Practice ; Humans ; Infant ; Meta-Analysis as Topic ; Milk Hypersensitivity ; diagnosis ; prevention & control ; Milk Proteins ; adverse effects ; Practice Guidelines as Topic ; standards ; Professional Staff Committees ; organization & administration

Child ; Humans ; Incisor ; abnormalities ; Maxilla ; Occlusal Adjustment ; Orthodontic Extrusion ; Tooth Abnormalities ; diagnosis ; etiology ; therapy ; Tooth, Impacted ; diagnosis ; etiology ; therapy

[Abstract ] Objective Explore the clinical pathological characteristics and diagnostic methods on ent mucous epidermoid carcinoma.Methods Collection of 20 cases with ent mucous epidermoid carcinoma in our hospital,using immunohistochemical method to detect patients with CK and EMA,CEA and CK8 ex-pression.Results Twenty patients were men,of which the high differentiation of 17 cases,3 cases of poorly differentiated.CK ,EMA and CEA in patients with tumor tissue and CK8 were positive expression. Conclusions Ent mucous epidermoid carcinoma is a kind of relatively rare ent tumor,its clinical patholog-ical features and tumor tissue in CK and EMA,CEA and CK8 expressed help to identify and diagnosis.

The essence of thinking was the procedure for processing information by knowledge in the frame of world view,which depending on the world view and the existing knowledge and information.The characteristic of traditional Chinese medicine (TCM) thinking was reflected by its unique world view.The world view of TCM thinking was natural view,holism concept and dialectic view,compared with modern scientific thinking mostly in reduction analysis.To develop TCM,the modern TCM thinking system was established through the reforming and innovating of TCM thinking characteristic based on preservation and promotion of them.

This study aimed at exploring the modeling and identification method of autoimmune POF by the ZP3 polypeptide with the provision of scientific basis for the prevention and treatment of POF.B6AF1 female mice,the hybridization of A/J male mice and C57BL/6 female mice,was immunized by zona pellucida polypeptide fragments.Then,the mice estrous cycle was detected by vaginal exfoliated cells Pasteur's staining.After the 14-day modeling,the mice model was identified on the 7th and 14th day,respectively.Ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change were detected by H&E staining and ZP3 immunofluorescence staining.Serum hormone levels of E2 and FSH in the mice were tested by ELISA,with the combination of estrous cycle,ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change to judge the success of the POF mice model.It was found that ZP3 polypeptide immunized mice appeared the disorder of estrous cycle after 14 days,so did the marked inflammation observed by ovarian biopsy,a clear transparent zone by ZP3 immunofluorescence staining,and the decrease of serum E2 declined and the increase of serum FSH by ELISA.However,no significant change was found on the 7th day in the modeling.In conclusion,ZP3 polypeptide induced autoimmune POF mice model was established successfully,featuring high incidence and simple method and laying a foundation for the further study of autoimmune POF.

OBJECTIVE:The bioassay and HPLC methods were established and compared for the determination of levofloxacin(Lev) in human plasma.METHOD:In bioassay,medium Ⅱ and staphylococcus aureus were used.The HPLC method was chromatographed with a mobile phase of methanol-0.02mol/L potassium dihydrogen phosphate solution (3∶7)，the sample was treated by adding 30% trichloroacetic acid to precipitate protein,and detected at 276mm.RESULTS:The average recovery of bioassay was within 95.90%～106.68%,the within-day and day-to-day precisions were below 6%.The average recovery of HPLC was within 97.24%～101.70%,the within day and day-to-day precisions were blow 5%.Lev tablets were given to 6 patients in two ways.Drug concentrations in plasma were determined by bioassay and HPLC assay,respectively,with no significant difference (P＞0.05).CONCLUSION:The bioassay was simple,cheap,time saving,and reliable.The HPLC method wsa rapid,sensitive,accurate.The two methods may be selected to use in actual conditions.