Objective To investigate the clinical advantages and safety profile of the combinational treatment of acute progressive cerebral infarction with aspirin,clopedogrel and atorvastatin.Methods 170 cases with acute progressive cerebral infarction were randomly divided into the treatment group and control group,85 patients in each group.Both two groups were initially treated with aspirin and atorvastatin.After excluding the possibility of hemorrhage, the treatment group additionally received clopedogrel,while the control group was treated with aspirin and atorvastatin. The difference in clinical efficacy was evaluated between before treatment and 7 days,14 days or 28 days after treatment by using the NIH Stroke Scale (NIHSS)and Barthel Index (BI).Results The NIHSS scores of the treatment group after 7d,14d and 28d were (12.52 ±3.25)points,(9.10 ±3.21)points and (6.87 ±2.85)points, which of the control group were (13.65 ±2.93)points,(10.73 ±3.41)points and (9.07 ±2.96)points respectively, the differences between the two groups were statistically significant(t =2.340,3.170,4.877,all P <0.05).The BI scores of the treatment group after 7d,14d and 28d were (35.26 ±11.18),(53.73 ±13.74)and (74.61 ±17.35), which of the control group were (31.98 ±9.12),(46.65 ±11.79 )and (63.87 ±15.73 )respectively,the differences between the two groups were statistically significant(t =2.131,3.752,4.456,all P <0.05).The overall effective rates of the treatment group after 7d,14d and 28d were 62.7%,79.5% and 94.0%,which of the control group were 51.2%,68.3% and 84.1% respectively.The differences of overall effective rates were statistically significant after 14d and 28d(χ2 =4.711,8.531,all P <0.05).Few reverse reactions were observed in both two groups.Conclusion Compared with the aspirin and atorvastatin therapy,combinational treatment of acute progressive cerebral infarction together with clopedogrel has a better efficacy,safety profile and significant promotion on neurological recovery.

Objective:To isolate and identify the periodontal ligament stem cells.Methods:Periodontal ligament tissues were digested with a mixture of collagenase and Dispase,then the periodontal ligament stem cell clones were isolated by limited dilution method.The transmission electron microscopy(TEM),flow cytometery and immunohistochemistry procedure were employed to study the ultrastructure,cell cycle and surface marker of the cloned cells.Results:The obtained cells showed the characteristics of undifferentiated morphology at ultrastructural level.94.4% of the cells were in phase G_0/G_1.The cells were Vimentin,STRO-1,ON and BSP positive.Conclusion:Cloning incubation may be the effective way to isolate and purify the periodontal ligament stem cells.

OBJECTIVE:To observe the effect of Xuezhikang on serum MCP-1 and endothelial function of brachial artery in patients with chronic heart failure but with normal cholesterol level. METHODS:Patients with chronic heart failure (NYHAⅡ-Ⅳ) were randomly allocated into two groups(n=24 each). Both groups received routine treatment in accordance with the guide of CHF treatment,additionally,the treatment group received Xuezhikang 0.6 g bid for 8 weeks. Serum levels of MCP-1 before and after treatment were measured by ELISA and endothelial function of brachial artery was detected by ultrasonography. RESULTS:In Xuezhikang-treated group compared with the control group,serum level of MCP-1 decreased significantly(P

Objective To observe the therapeutic effect and security of Thermal Moxibustion combined with Yougui Pills in the treatment of premature ovary failure(POF) of spleen and kidney Yang-deficiency.Methods One hundred and forty-two patients with POF of spleen and kidney Yang-deficiency were randomly divided into the treatment group and control group,71 cases in each group.The observation group were treated with Thermal Moxibustion combined with Yougui Pills,while the control group took oral Yougui Pills,12 weeks as a course of treatment.After treatment,the clinical therapeutic effects,K upperman score,related hormones levels(E2,FSH,LH) and occurrence rate of adverse reactions were compared between the two groups.Results The total efficacy rate of the observation group was 95.77％,which of the control group was 77.46％,and the observation group was significantly better than the control group(P＜0.05);there were significant improvement in the Kupperman score and levels of related hormones after treatment in the two groups(P＜0.05),but the improvement degree of the observation group was superior to the control group(P＜0.05).Conclusion Thermal Moxibustion combined with Yougui Pills can alleviate the clinical symptoms with high safety and higher clinical application value

Objective To Investigate the disorder of colonic epithelium apoptosis and proliferation as well as its role of the epithelial barrier break down in ulcerative colitis(UC). Methods Fifty biopsy specimens were obtained endoscopically from 35 patients of UC and 15 controls respectively.The specimens were used for diagnosis of UC and investigation of epithelium proliferation an d apoptosis by IHC with markers of ki－ 67 and M30 respectively. Results Apopto sis index(marked by M30) and ratio of apoptosis to proliferation increased obvio usly in UC compared with the controls(P0.05);Disorder of epithelium apoptosis an d proliferation take place in UC.Apoptosis cells were found not only at luminal surface but also at the base of crypt;however,proliferation cells may extend to superior part of the crypt. Conclusion Unlimited and premature apoptosis as wel l as disorder of apoptosis to proliferation is maybe one of the causes which lea ding to breakdown of the epithelial barrier function i n UC.

This paper reported a new method of Armillaria mellea isolation-Gastrodia elata tissue isolating. Compared with normal isolating method-rhizomorph isolating method, it showed that the success rate of new method (78% ) was higher than the rhizomorph isolating method (16% ) , besides this, the new method was easier, and growth characteristic of obtained strain was superior to that obtained from rhizomorph isolating method.

A coupling principle of reflecting impedance based on resonance is designed to achieve impedance detection with high sensitivity. It is characterized by small impelled current, high sensitivity and simple circuit. The principle can be used not only in detecting human respiratory impedance, but also in detecting the bio-impedance of other human organs. It may find wide application in this aspect.
Electric Impedance ; Equipment Design ; Humans ; Respiratory Function Tests ; instrumentation ; methods ; Software Design

Objective To evaluate the effect of FOCUS- PDCA model in hypoglycemia management during hemodialysis in patients with diabetic nephropathy. Methods Totally 107 patients with diabetic nephropathy were divided into two groups. Patients in the control group received traditional hypoglycemia management, while FOCUS- PDCA model was implemented in the experimental group in hypoglycemia management, including reducing insulin dosage before hemodialysis,injecting 50%glucose and taking hyperglycemia food during hemodialysis,enhancing the monitoring of blood glucose and health education. Compared the incidence of blood glucose levels and hypoglycemia in two groups of patients at different time of dialysis. Results The frequency of hypoglycemia in the experimental group at the beginning of dialysis [ (9.04±2.08) mmol/L ], 2 hours after dialysis[ (5.78±1.82) mmol/L ]and at the end of dialysis[ (6.94±2.04) mmol/L ] were significantly lower than that in the control group[ (8.60±2.02) mmol/L, (5.20±1.62) mmol/L, (4.97±1.05) mmol/L ], (P <0.05), and the total incidence rate of hypoglycemia was 1.6%(61/3 889), which significantly lower than that 4.8%(185/3 816) in the control group, the difference was statistically significant (χ2=5.288, P < 0.05). Conclusions The application of FOCUS- PDCA model in the hypog1ycemia management of patients with diabetic nephropathy can reduced the incidence of hypoglycemia during hemodialysis. It deserves to be widely used in clinical practice to improve the patients′quality of life.

Objective To study the effect of neuroepithelial cell transforming gene 1 (Net1) on the cellular radiosensitivity and underlying mechanism.Methods Real-time quantitative PCR was used to measure the variations in Net1 expression level upon irradiation.Radiosensitivity was analyzed by colonyforming assay after Net1-siRNAs.Net1-associated proteins were identified by co-immunoprecipitation.Results The Net1 mRNA level in the cells was increased significantly (t =-10.52,P ＜ 0.05) after irradiation.Compared to the control group,siRNA-mediated silencing of Net1 enhanced cell radiosensitivity (t =15.31,11.65,P ＜0.05).Net1 was found to interact with Ku70,Ku80 and DNA-PKcs under either normal conditions or after irradiation.Conclusions Net1 could protect cells from irradiation by interaction with DNA repair proteins in non-homologous end joining pathway.