OBJECTIVE:To explore the effect of vinblastine(VBL)on the proliferation and nitric oxide(NO)secretion of RAW264.7 cells. METHODS:MTT and flow cytometry were used to evaluate the inhibition effect of VBL on the proliferation of RAW264.7 cells and blockade effect of VBL on cell cycle distribution,respectively. The change of protein expression of cyclin B1 was detected by Western blot method. Griess kit was adopted to test the effect of VBL on LPS- induced NO secretion of cell. RESULTS:VBL can inhibit the proliferation and NO secretion of RAW264.7 cells,arrest cells in G2～M phase and reduce the levels of cyclin B1 in a concentration- dependent manner(P

Objective:To observe the influence of sidenafil citrate on the ovarian and uterine artery blood flow in the infertile women. Methuds:In the cases of IUI changes of the ovarian and uterine artery s pulsatility index(PI) and resistance index(RI) ,were observed using transabdominal color Doppler flow image before and after treatment with sidenafil citrate (Viagra) .The PI and RI were inspected and the uterine endometrial development was examined simultaneously. Results: The PI and RI of the ovarian and uterine artery blood flow was decreased significantly by sidenafil citrate (P

Objective:To assess the role of the estrogen level per ovum in the clinical pregnancy rate in IVF-ET.Methods:Serum estrogen levels on the day of the HCG(human chorionic gonadotrophin)administration were assayed by EIA(Magnetic Solid Phase).Results:When the estrogen level per ovum was 70～140 pg/ml on the day of HCG administration,the pregnancy rate was the highest.Conclusion:The estrogen-oocyte ratio is a strong index of success pregnancy rate in IVF-ET.

Objective:To study the effect of polycystic ovary-like environment on secretion of E_2 and P from human granulosa luteal cells in vitro.Methods:According to different additives granulosa cells cultured in vitro which were retrieved from infertility women with normal ovulation who were undergoing in vitro fertilization-embryo transfer(IVF-ET),were divided into four groups:A (androstenedione) group,LH group,INS (insulin) group and A+LH+INS group.Each group was subdivided into polycystic ovary-like environment group with higher levels of the additives and physiological environment group with lower levels of the additives.Levels of E_2 and P in medium were detected separately.Results:Levels of E_2 and P in polycystic ovary-like environment were significantly decreased in A group and A+LH+INS group,and significantly increased in LH group and INS group (P

Objective·To investigate the correlation between the serum haptoglobin (Hp) level and the metabolic parameters in patients with polycystic ovarian syndrome (PCOS), and explore the distribution of Hp genotype and allele frequency in PCOS patients and their correlations with PCOS. Methods·A total of 106 patients with PCOS and 86 cases of patients with non-hyperandrogenic and regular menstruation (control group) were enrolled. Serum Hp level was measured by enzyme-linked immunosorbent assay (ELISA); Hp genotype and allele frequency were determined by polymerase chain reaction (PCR), respectively. Results·The serum Hp level in PCOS group was significant higher than that in the control group (P=0.000). Correlation analysis reveals that serum Hp level was positively related to body mass index (BMI) (P=0.000) and homeostatic model assessment insulin resistance (HOMA-IR) (P=0.000). Compared with control group, proportion of Hp2-2 genotype (Hp2/Hp2) increased significantly in PCOS group (P=0.006), and the frequency of Hp2 allele was also higher (P=0.002). Conclusion·Serum Hp level was positively related to BMI and HOMA-IR, which maybe associated with the occurrence and development of obesity and insulin resistance. Patients with Hp2-2 genotype might have genetic predisposition to PCOS, and high frequency Hp2 allele was found in them.

Objective To evaluate the feasibility of application of video-laparoscopy to the treatment of acute severe inflammatory pelvic pain in women. Methods Forty-two women patients with severe acute inflammatory pelvic pain were divided randomly into two groups with 21 patients in each group. Conservative therapies were carried out in Group A while laparoscopic operations in Group B. Results Eighteen cases (85.7%) in Group A returned to health, and in the remaining 3 (14.3%), conservative measures failed and thus exploratory operations were performed. Meanwhile all the patients in Group B were treated successfully, with the operative time (42.5?20.6) min, the average blood loss 20ml and the mean hospital stay 7 days. Body temperatures returned to normal on the day of operation and no complications occurred. Conclusions Laparoscopy may be feasibly applied to the treatment of women with severe acute inflammatory pelvic pain, producing results superior to conservative therapies.

Hepatocellular carcinoma (HCC) is the fifth most common cancer and the second leading cause of cancer-related death worldwide.Increase in incidence has been shown in HCC over the last few years and more than half of the cases were reported in China,where hepatitis B virus (HBV) infection is the main etiologic factor.Heterogeneity in HCC's distribution worldwide and difference in etiology may result in a more complicated issue about prognosis-estimation and choosing of treatment options for HCC patients.In the past decade,there have been several clinical staging systems developed in terms of relevant prognostic factors.Among them,the Barcelona Clinic Liver Cancer (BCLC) and the Hong Kong Liver Cancer (HKLC) staging system are the only two classifications that link prognostic classification to treatment indications.In this review,we mainly focus on the application of BCLC and HKLC staging systems in guiding decision making for HCC,the respective advantages and disadvantages of each classification,and the future perspective of our own prediction model in which some new clinical factors will be integrated.

Objective To study the effect of letrozole (LE) on ovulation in patients with polycystic ovary syndrome (PCOS).Methods One hundred and sixty-four patients with PCOS were divided into LE treatment group (n=68) and chloramiphene (CC) treatment group (n=96).Growth rate of follicles,serum hormone level,endometrial thickness,and cervix mucus were observed before and after treatment.Results The growth rate of follicles was 1.67 and 1.78 mm/d,respectively,in LE and CC treatment groups (P=0.14).The serum T level on the day of LH peak was 2.1?1.9 and 2.0?1.7ng/dL,respectively,in LE and CC treatment groups (P=0.93).The serum testosterone (T) level on day of LH peak and on day 3 of menses was 2.1?1.9 and 1.4?1.5 ng/d,respectively,in LE and CC treatment groups (P=0.09).The serum E2 level on day of LH peak was 89.0?59.5 and 108.1?46.0 pg/ml,respectively,in LE and CC treatment groups (P=0.13).The endometrial thickness was 11.3?1.6 and 9.7?1.4 mm,respectively,in LE and CC treatment groups (P=0.07).The score of cervix mucus was 11.0?1.1 and 8.3?3.1,respectively,in LE and CC treatment groups (P=0.003).Conclusion Letrozole can effectively induce ovulation in patients with polycystic ovary syndrome,and does not increase serum T level.

Objective To explore the effects of dexamethasone on ovulation induction in polycystic ovary syndrome (PCOS).Methods This clinical study included 68 women with PCOS, which of them were divided into two groups: 34 in dexamethasone treating(dexamethasone combined with ovulation induction) and 34 in con-trol(ovulation induction treatment only).Subsequently, comparisons were made between pre and after drug treat-ment in two groups, the main indexes were the levels of testosterone(T), luteinizing hormone(LH), follicle stim-ulating hormone(FSH), growth rate of follicle, and body mass index(BMI).And we have also considered the o-vulation rate and pregnancy rate, and compared these indexes in dexamethasone treating group with control group.Results There were significant differences between pre and after dexamethasone treatment in the levels of T in treatment group(P < 0.01), and there were not significant differences between pre and after treatment in the lev-els of T in control group (P >0.05).LH had decreased significantly after treatment in both two groups (P <0.01), FSH had not significant differences between pre and after treatment in two groups(P >0.05).The growthrate of follicle of two groups had elevated after treatment (P < 0.01).And the ovulation rate in dexamethasone treating group was higher than that in control group(P < 0.05), but there were not significant differences between the two groups in the clinical pregnancy rates(P >0.05).There were not significant differences between pre and after treatment in the levels of BMI in both two groups (P > 0.05).Conclusions Dexamethasone can decrease the T in plasm levels.It is good for ovulation induction in PCOS.

Objective: To investigate the effect of NS-398, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation and invasion of human colon carcinoma cells in vitro, so as to determine the possibility of COX-2 as a new target for treatment of colon carcinoma. Methods: The expression of COX-2 in colorectal cancer cells (CW-2, COLO-320) was detected by RT-PCR and Western blotting. COLO-320 cell proliferation was measured by MTT after treatment with NS-398. Cell invasion ability was measured using migration and invasion chamber systems. Western blotting assay was used to examine the influence of NS-398 on MMP 2 expression. Results: Our results showed that CW-2, COLO-320 cells expressed COX-2 mRNA and protein. NS-398 inhibited the proliferation of COLO-320 cells in a time and concentration-dependent manner. Invasion test showed that NS-398 inhibited the migration and invasion of COLO-320 cells. Western blotting revealed that NS-398 inhibited the expression of MMP-2 in COLO-320 cells. Conclusion: The selective COX-2 inhibitor NS-398 can inhibit COLO-320 cell proliferation and invasion, indicating COX-2 may serve as a new target for colon carcinoma treatment.