Adult ; Female ; Humans ; Male ; Middle Aged ; Pesticides ; poisoning

Adult ; Female ; Humans ; Middle Aged ; Nurses ; Occupational Exposure ; prevention & control ; Operating Rooms ; Surveys and Questionnaires ; Universal Precautions ; Young Adult

Atherosclerosis is the important pathologic basis of coronary artery disease,cerebrovascular disease,peripheral arterial disease and other vascular diseases.Studies have shown that the atheromatous plaques occur mainly in regions of curvature,bifurcation,and branching of the artery,indicating that low flow shear stress is closely associated with the occurrence of atherosclerosis.Low flow shear stress involves in the induction of atherosclerosis through acting on mechanosensitive molecules in endothelial cell that transduce mechanical stimuli into chemical signals,activate the corresponding intracellular signaling pathways.This article reviews the relationship between low flow shear stress and atherosclerosis.

Adult ; Diagnosis, Differential ; Electrocardiography ; Female ; Humans ; Myocardial Infarction ; physiopathology ; Pheochromocytoma ; physiopathology

Objective To study the mechanism of hyperintensed signal at interpeduncle region in meseneephalon on DWI with diffusion sensitive gradient(Gd)applied perpendicular to the slice direetion (DWIs).Methods MRI scanning was centered at interpeduncle region.and the protocols were as follows:(1)With electrocardiograph(ECG)triggering,DWIs was acquired during systole or diastole periods respectively.(2)To observe the shape of hyperintense signal,DWIs with various slice directions was applied,which was set parallel to skull base,along Y axis,or along the oblique line between Y and Z axis with 30°to Z axis.(3)Fiber tracking was performed with the hyperintense signal as"seed"region,fractional anisotropy(FA)and ADC were derived from(multiple directions diffusion weighted imaging,MDDW).FA and ADC of hyperintease region were compared with those of the nearby fiber by using pair t-tesL Results Signal intensitv of hyperintense region on DWIs were 2296.28±38.19 and 153.81±37.91 for b=0 and b=1000 at systole period.while they were 295.36±38.84 and 154.03±37.52 at diastole period.No staffstical difference wag demonstrated between them(t=1.34,0.62,P＞0.05).The ADC valus on DWIs acquired during systole and diastole periods were(6.07±2.20)×10-4and(6.69±1.44)×10-4 mm2/s respectively.There was rio statistical difference between them(t=0.94,P＞0.05).DTI fiber tracking verified that the hyperintense region located at the decussation of superior eerebelum peduncle(SCP)in mesencephalon.It was in long and narrow heart shape or rectangular shape on DWIs.The shape depended on the direction of Gd The ADC value derived from MDDW at hyperintense region and the fiber below were(10.61±3.42)×10-4 and(9.24±2.21)×10-4 mm2/s respectively.No statistical difference was demonstrated between them(t=0.61.P＞0.05).While,the FA at hyperintensed region 0.43±0.13 was less than that of the fiber below 0.61±0.08(t=8.32.P＜0.05).Conclusions The shape of the hyperintense region on DWIs depends on the direction of Gd.The cardiac period has no effects on the hyperintense signal at peduncles,which may be attributed to the anisotropy of SCP decussation in mesencephalon.

Objective To explore the application of evaluation of measurement uncertainty in routine coagulation assays based on the data of internal quality control (IQC) and results of external quality assessment (EQA) .Methods Data of clinical coagulation assays of clinical laboratory of Xiangyang city hospital were collected ,Which came from six months of IQC and 3 times of EQA of ministry of health clinical laboratory center from 2012 to 2013 .The combined and expanded uncertainties of 3 measurements(PT , APTT and Fbg) were evaluated according to CNAS top-downapproach .Results When PT were in 12 .72 s and 21 .04 s ,its ex-panded uncertainty were 1 .06 s and 1 .96 s ,and APTT in 36 .6 s and 50 .6 s were 2 .59 s and 5 .30 s ,and Fbg in 3 .32 g/L was 0 .30 g/L .Conclusion Evaluation of measurement uncertainty by using internal quality control data and EQA results for routine coagula-tion detection index is economical and practical and acceptable way ,with good clinical practice .

Objective To acquire the physiological parameters such as pulse,blood oxygen saturation and ECG to realize personal health information monitoring and tailored medical service.Methods A personal health information acquisition terminal was developed,which was composed of an information acquisition module,personal computer and system software.The information acquisition module was developed with USB interface and unified circuit mode.Health information was transmitted to the personal computer at real time with USB interface,and then stored by system software and uploaded to the database.Results The terminal gained advantages in design,convenience and practicability,which realized realtime acquisition,data storage,remote auxiliary diagnosis,printing and etc of personal health information,so that personal information realtime monitoring was implemented.Concluslon The terminal can be used for health information recording,storage,acquisition and analysis,and extends medical service of hospital.

OBJECTIVETo investigate whether there is an association between DRD2/ANKK1 Taq IA polymorphism and early infant temperament.

METHODSDRD2/ANKK1 Taq IA polymorphism (rs1800497) was determined using polymerase chain reaction-ligase detection reaction (PCR-LDR) techniques in 149 Chinese Han infants from Changsha City. Their mothers were asked to complete the Early Infant Temperament Questionnaires (EITQ) when the infants were 1 to 4 months old (mean: 2.75 months). There were three genotypes found in these infants: C/C, T/T and C/T. The subjects were subdivided into T-carrier (CT, TT) and non-T-carrier (CC) groups for statistical analysis.

RESULTSThere were no differences in the temperament style distribution between the T-carrier and non-T carrier groups. There were also no statistically significant differences between the two groups in the score of the nine temperament dimensions.

CONCLUSIONSDRD2/ANKK1 Taq IA polymorphism is not associated with early infant temperament.

AIM: To confirm the differential expression genes in the rat ischemic brain. METHODS: The middle cerebral artery occlusion ischemic model was set up in rats. Fluorescence differential display reverse transcriptase-polymerase chain reaction (FDD RT-PCR) and reverse Northern blotting were used to fast confirm the differential expression genes. RESULTS: Nine differential expression sequence tags, including 6 known sequences and 3 unknown sequences, were confirmed. Among the known sequences, mus musculus ab1-interactor1,homo sapiens CGI-99 protein, tissue inhibitor of metalloproteinase 3 and homosapiens nuclear receptor co-repressor were up-regulated while homo-sapiens nuclear matrix protein p84 and coatomer protein complex, subunit gamma 2 were down-regulated. CONCLUSIONS: ① Combination of fluorescence differential display reverse transcriptase-polymerase chain reaction (FDD RT-PCR) and reverse Northern blotting is a method to fast-confirm the differential expression genes; ② There are differential expression genes in ischemic brain regions compared to non-ischemic parts. [

AIM: To Screen and identify differentially expressed genes that involved in apoptosis model in rat cerebellar granule neurons (CGNs).METHODS: The rat cerebellar granule neurons were isolated and primarily cultured. Fluorescent differential display RT-PCR (FDD RT-PCR) was performed to screen differentially expressed ESTs in the apoptosis model of primarily cultured rat CGNs. ESTs were subcloned into pGEM-T EasyTM vector and then sequenced. Alignment assay in non-redunant database was applied for encoding information. Reverse Northern blotting was used to appraise the results from DDRT-PCR.RESULTS: 164 pieces of differentially expressed ESTs were obtained by FDDRT-PCR. 17 of them were subcloned and sequenced. 5 ESTs of 17 were confirmed to be positive results by reverse Northern blotting. CONCLUSION: DD-PCR is a rapid, simple-operation and sensitive method for screening differentially expressed genes, which would contribute to the molecular mechanisms of apoptosis/survive of CGNs.