Objective:This study is to investigate the job satisfaction of nursing staff in public hospital and ana-lyze the influential factors, thus to provide evidence for improving the performance of human resource management in the hospitals. Methods:A sample of 100 nurses in the affiliated hospital of Weifang Medical University was se-lected to participate in this study with cluster sampling method. Job satisfaction was investigated using self-de-signed questionnaire, and data analysis was conducted with SPSS software. Results:The total score of job satisfac-tion is(92. 52 ± 18. 17). The highest is about their working group with 3. 89 ± 0. 58, while the lowest is about their labor return with(2. 19 ± 0. 89). Authorized strength, education, working years and monthly salary all impact the job satisfaction of nurses. Conclusions:At present, nurses′job satisfaction is at a media level. The managers of public hospitals should build a scientific compensation system and humanized duty system and so on, which can meet the different needs of nursing staff and improve their job satisfaction.

Objective To investigate the change of Gadd45?/? in HepG2 cells after UV radiation.Methods HepG2 cells were cultured in vitro followed by radiation with UV (254nm,10 J/m2)and harvested at 1,2,4 h respectively.The induction of Gadd45?/? mRNA by UV was analyzed using RT-PCR,and the induction of Gadd45?/? protein was determined by Western blot assay at the given interval simultaneously.Results The peak values of Gadd45?/? mRNA appeared at 4 h and 1h after induction respectively,while the both of the peak value of Gadd45?/? protein were found at 1h after UV radiation.Conclusion Gadd45?/? could be rapidly induced by UV radiation in HepG2 cells.

Objective To explore the association of activated blood platelet and large vacular disease in type 2 diabetes mellitus.Methods A total of 98 cases of type 2 diabetes mellitus patients were divided into two groups(50 cases with large vascular disease and 48 cases,as DM group)and 50 cases healthy subjects served as a control group.From 2002-05 to 2004-11,the doctors of the Department of Endocrinology,Subei Hospital to determined the expression of platelet activation PAC-1、CD62P in large vascular disease by technique of flow cytometry(FCM),and glycosylated hemoglobin(HbA_1c)、C-reactive protein(CRP)、fasting plasama glucose(FBG)、C-peptide(C-P)and blood lipids were measured and analysed for the correlation.Results Activated blood platelet was significantly increased in DM group as compared with that in control group(P

Objective To investigate the expression of Gadd45beta protein in human hepatocellular carcinoma(HCC) and its clinicopathologic significance.Methods Immunohistochemical method was used to detect the expressions of Gadd45beta in both hepatocellular carcinoma tissues and adjacent non-neoplastic liver tissues form 50 cases.Results The positive rates of Gadd45beta expression in the hepatocellular carcinoma tissues and adjacent non-neoplastic liver tissues were 26.0%(13/50) and 82.0%(41/50) respectively.There were significant difference for the positive rate between the two groups(P0.05).Conclusion Low expression of Gadd45beta protein in hepatocellular carcinoma tissues was exhibited,which may be related to hepatocarcinogenesis as well as biological behaviors and prognosis of HCC.

ObjectiveTo examine the mental health status and its changes among children exposed to the Wenchuan earthquake 2 and 3 years after it has passed,and evaluate the possible influencing factors of mental health.MethodsThe General questionnaire,the self-reported Children's revised impact of event scale(CRIES)and the Depression self-rating scale for children ( DSRSC ) were administered to the same children from the earthquake affected areas 2,3 years after the earthquake.ResultsBoth of the CRIES scores and DSRSC scores had decreased over the 2,3 years periods after the earthquake ( z =- 8.772,P =0.00; z=- 2.870,P =0.00 ).The risk factors associated with PTSD 3 years after the earthquake were:gender (OR =1.069,P =0.00 ),the severity of their family's injury( OR =1.141,P =0.02 ),the length of living in transferred safe places( OR =1.416,P =0.03 ),and whether or not witnessed collapse ( OR =1.014,P =0.01 ).The risk factors associated with depression 3 years after the earthquake were:gender( OR =1.367,P =0.00),age( OR =1.035,P =0.04),and despair( OR =2.490,P =0.01 ).ConclusionBoth of the symptoms of PTSD and depression among the children from earthquake affected areas have decreased over the 2,3 years periods after the earthquake.The mental health status of the children from the earthquake affected areas are influenced by their gender,age,the length of living in transferred safe places,the severity of their family's injury,whether or not witnessed collapse and despair.These factors should be taken into considerations when the long-term post-traumatic psychological interventions are planned.

Objective To investigate motor nerve function status in rats with diabetes mellitus by motor unit number estimation (MUNE), and discuss it′s early diagnostic value in diabetic peripheral neuropathy (DPN). Methods Diabetic rat model (DM group) was induced by streptozotocin. The MUNE of gastrocnemius muscle and motor nerve conduction (MCV, CMAP) of the sciatic nerve were measured at the 4th, 8th and 12th week after onset of hyperglycemia in the DM group and the control group (normal SD rats). The ultrastructure of sciatic nerve was observed by electron microscope. Results At the 4th week, MUNE of gastrocnemius muscle was significantly decreased in DM group compared to that of the control group (275.88 ± 87.87 vs 369.71 ± 75.64,P<0.05). There were no significant differences in MCV and CMAP of sciatic nerve be?tween two groups. The electron microscopy observation showed that most nerve fibers were normal;a small amount of axonal atrophy, and myelin lamellar structure was separated in DM group. At the 8th week, compared with the control group, MUNE were reduced in gastrocnemius muscle in DM group (357.49±72.68 vs 221.26±92.41, P<0.01). There were no significant dif?ferences in MCV and CMAP of the sciatic nerve between DM group and control group. The electron microscope observation showed that part of nerve fibers were normal, the myelin focal plate layer was loose and separated, axonal atrophy, the axonal membrane and myelin sheath inner layer was separated with big gap. At the 12th week, MUNE of gastrocnemius muscle (127.87±19.80 vs 366.85±51.25), sciatic nerve MCV [(35.06±4.43) m/s vs (50.47±6.07) m/s] and CMAP [(2.91±1.37) mV vs (5.98±2.14) mV] were significantly decreased in DM group than those of control group (P<0.01). The electron microscopy observation showed severely damaged myelin flex and axonal squeeze. Conclusion MUNE is much earlier in detecting ear?ly motor nerve dysfunction in DM than conventional motor nerve conduction test.

Objective To assess the function of motor nerve fiber in patients with diabetic peripheral neuropathy (DPN) by single-fiber conduction studies.Methods According to the diagnostic standard of DPN issued in Toronto meeting in 2009,on the basis of the result of peroneal nerve conventional conduction study,a total of 65 patients with DPN in the Department of Endocrinology and the Department of Neurology of Tianjin Third Central Hospital from October 2012 to October 2013 were enrolled into the study,from whom 33 had abnormal sensory conduction (sensory-diabetic peripheral neuropathy group,S-DPN group),32 had abnormal sensory motor conduction (sensory motor-diabetic peripheral neuropathy group,SM-DPN group).Single-fiber conduction velocity (SF-CV) and single-fiber distal motor latency (SF-DML)were detected in all subjects.The obtained results were compared with the data from 34 healthy volunteers (control group).The relationship of SF-CV,SF-DML and the duration of diabetes mellitus,fasting glucose,HbA1 c was also studied in DPN patients.Results The SF-CV ((43.1 ± 3.6) m/s) was decreased in S-DPN group compared with control group ((47.5 ± 3.3) m/s,t =5.077,P ＜ 0.01).There were no significant differences in SF-DML ((3.6 ± 0.7) ms),motor nerve conduction velocity (MCV (49.5 ± 2.6)m/s) and DML ((3.4 ± 0.6) ms) in S-DPN group compared with that of control group ((3.4 ± 0.5) ms,(50.9 ± 3.5) m/s,(3.2 ± 0.5) ms,respectively).SM-DPN group had lower SF-CV ((35.2 ± 3.6)m/s,t =9.119,14.219),MCV ((40.9 ± 3.2) m/s,t =11.131,13.025) and increased SF-DML ((4.5±0.7) ms,t=5.692,7.231),DML ((4.2 ±0.7) ms,t=5.561,6.975) compared with the other two groups (P ＜0.01).SF-CV in DPN patients was negatively related to the diabetic duration (r =-0.340,P =0.006),while SF-DML had no correlation with duration of DM,fasting blood glucose and HbAlc.Conclusions Detection of SF-CV is easy to find early motor nerve dysfunction in DPN patients.SF-CV is decreased with the increasing duration of diabetes.

Background and purpose:High mobility group 1 (HMGB1), frequently found to be over-expressed in many human tumors, plays an important role in tumor progress and metastasis. This study aimed to investigate the mechanism of HMGB1 promoting A549 cell metastasis.Methods:A549 cells were untreated or treated with HMGB1 (200 ng/mL) in absence or presence of NF-κB inhibitors 6-amino-4-quinazoline (QNZ, 40 nmol/L) or Bortezomib (Bort, 20 nmol/L). Scratch assay and Transwell assay were performed to evaluate A549 cells migration and invasion ability. The activity of NF-κB was examined by luciferase reporter assay. NF-κBp65 and αvβ3 expressions were detected by Real-time RT-PCR or Western blot.Results:HMGB1 increased A549 cells migration and invasion ability. HMGB1 enhanced NF-κB protein level and NF-κB activity in A549 cells. Real-time RT-PCR and Western blot showed that HMGB1 up-regulated αvβ3 expression in A549 cells. NF-κB inhibitors QNZ or Bort reserved the promot-ing effects of HMGB1 on A549 cells migration and invasion, NF-κB expression and activity as well as αvβ3 expression. Conclusion:HMGB1 promotes A549 cell migration and invasion through activating NF-κB and up-regulating αvβ3.

Objective To discuss the effects of extract of Ginkgo BilobaLeaves(EGb) on expression of cytokine of renal interstitial fibrosis induced by transforming growth factor-?1 (TGF-?1) and extracellular matrix. Methods Cultured human kidney cells(HKC) were divided into three groups: control group,TGF-?1(8 ng/mL) group,and TGF-?1(8 ng/mL) added EGb(25,50,100,150 mg/L)group.After 72 h,expression of ?-SMA was detected by cell immunochemistry ABC,and collagen type I by Real-time PCR and Western blotting. Results Treated with TGF-?1(8 ng/mL) for 72 h,expression of ?-SMA and collagen type Ⅰ were up-regulated markedly compared with control group.Treated with EGb(25,50,100,150 mg/L)and TGF-?1(8 ng/mL)concomitantly for 72 h,expression of ?-SMA and collagen typeⅠ were down-regulated in dosage dependent manner compared with TGF-?1 group. Conclusion EGb can inhibit expression of ?-SMA and collagen type I in HKC induced by TGF-?1,and the possible mechanism might be related to the inhibition of EGb on renal tubular epithelial-myofibroblast transdifferentiation.

Adolescent ; Altitude ; Ghrelin ; blood ; Humans ; Overweight ; blood ; Sleep