Objective To evaluate the performance of the body fluid mode on the automatic analysis system-XT-4000i .Methods XT-4000i analyzer was used to detect the precision ,accuracy ,carryover rate ,meanwhile to validate the detection limit of the pa-rameters(WBC and RBC) .Results The range of the precision for WBC-BF was 0 .90% -5 .54% ,for RBC-BF was 1 .13% -5 .13% .The carry over rates were 0 .09% for WBC and 0 .14% for RBC ,respectively .As for as the analyzer range ,the results of the linearity and the detection limit verification were in line with the requirements of the analyzer ;the comparison between the man-ual microscopy results and the analyzer results meet the requirements of XT-4000i analyzer .Conclusion The performance of XT-4000i analyzer is in line with the requirements of the instrument range ,which can be used for clinical body fluid analysis and detection .

Objective To explore the clinical effect of probiotics combined with saline enema in the treatment of infantile diarrhea and its effect on serum inflammatory factors.Methods 186 cases of infantile diarrhea were selected,they were randomly divided into three groups according to the random number table method:probiotics group,montmorillonite group and control group.Patients in probiotics group were administered with clostridium butyricum combined with saline enema,and patients in the montmorillonite group were treated by montmorillonite combined with saline enema,and the control group was given routine treatment.Then,the clinical symptom relief time,total effective rate,serum inflammatory factor concentration and adverse reaction were observed and compared.Results The total effective rate of the probiotics group was 98.39%,and that of the montmorillonite group was 96.77%,both of which were significantly better than 80.65% of the control group,and the differences were statistically significant(χ2 =11.408,P =0.000;χ2 =8.432,P =0.018).After treatment,the serum levels of inflammatory factor tumor necrosis factor -α(TNF -α)in the probiotics group,montmorillonite group,control group were (8.30 ±2.33)pg/mL,(8.54 ± 2.47)pg/mL,(12.49 ±3.44)pg/mL,and the difference was statistically significant(F =5.120,P =0.014),also, the serum levels of interleukin -6(IL -6)in the three groups were (10.08 ±5.46)pg/mL,(11.24 ±5.84)pg/mL, (14.02 ±5.97)pg/mL,the difference was statistically significant(F =34.015,P =0.000).There were 2 cases of constipation in the montmorillonite group,the incidence rate was 3.23%,and there was no adverse reaction in the other two groups.Conclusion The effect of probiotics combined with saline enema in the treatment of infantile diarrhea is similar to that of montmorillonite,which can promote the children to alleviate the symptoms,shorten the length of hospital stay,reduce inflammation,and has no adverse reactions such as constipation,it is worth to be recom-mended widely for clinical application.

Anti-Bacterial Agents ; adverse effects ; Azithromycin ; adverse effects ; Chemical and Drug Induced Liver Injury ; etiology ; Humans ; Infusions, Intravenous

Aristolochic Acids ; adverse effects ; Drugs, Chinese Herbal ; adverse effects ; Humans ; Kidney Tubular Necrosis, Acute ; chemically induced ; prevention & control

Eighty-eight patients with obstructive sleep apnea hyperpnoea syndrome (OSHAS) were assigned to the mild (n =21 ), moderate (n =30), or severe group (n =37) according to apnea-hypopnea index( AHI ). Twenty-five healthy individuals were selected as normal controls. Color Doppler ultrasonography was used to measure the intimae-media thickness of the carotid coteries and the occurrence of sclerosis plagues. Our data found that increased severity of OSHAS led to the elevated intimae-media thickness of the carotid coteries and more sclerosis plagues, which suggests OSHAS may influence the development of the carotid artery arthrosclerosis.

Objective To explore the mechanism of cerebral infarction caused by hyperhomocystinemia.Methods A hundred and nineteen paitents with acute cerebral infarction were chosen for case group.According to their levels of plasm total homocystine,they were divided into two groups: hyperhomocystinemia group and nonhyperhomocystinemia group.Forty patents without cerebrovascular disease,hepatophy,nephrosis and thyroid gland disease were chosen as control subjects.Plasm levels of total homocystine,serum levels of MDA and IL-8 were measured respectively,their correlations were also studied.Results Plasma levels of tHcy(?mol/L)and serum levels of MDA(nmol/L)and IL-8 (ng/ml)showed a significant increase in case group(19.97,4.41?0.84,0.23?0.08)in comparison with control subjects(9.83,3.24?0.64,0.12?0.08),t values were 8.139,8.021,7.767 respectively(P

OBJECTIVE:To approach the correlations of carbamazepine concentration in serum with clinical therapeutic effect,dosage,sex,age and body weight in epileptic children.METHODS:158 monitoring records for serum concentration of carbamazepine in children with epilepsy in our clinical pharmacy department were consulted retrospectively.Data such as serum concentration,sex and age were recorded into data base and were analyzed using SPSS 13.0 software.RESULTS:No significant correlation was noted between serum concentration of carbamazepine in epileptic children and sex.However,serum concentration of carbamazepine was closely correlated with clinical therapeutic effect,dosage,age and body weight.CONCLUSION:The individual difference in the metabolic processes of carbamazepine was concerned with age and body weight,so it's necessary to make individualized regime according to child's specific condition and monitoring results.

Objective To observe the suppressive effect of combination of tissue plasminogen activator(t-PA),heparin and homoharringtonine on the formation of proliferative vitreoretinopathy (PVR) after vitreoretinal surgery. Methods Forty-three cases (44 eyes)of complicated retinal detachment who receivedvitreoretinal surgery were divided into 2 groups.Twenty cases(20 eyes)in group A were treated by intravitreal injection of above mentioned drugs at the end of operation,while no intraocular injection of drugs given in 23cases(24 eyes)in group B.The mean follow-up period was 7.9 months. Result The rate of recurrent PVR in group A was 15.8%(3 of 19),and 45.5%(10 of 22) in group B (P

Objective To investigate the reversal effect of abnormal savda munziq total flavonoids on leukemic cell lines.Methods CCK -8 method was used to detect the cytotoxicity of abnormal savda munziq total flavonoids on leukemia cells,by multivariate analysis of variance in the chemotherapy drug intervention,further calculated the reversal fold.Results CCK -8 results showed that with the increase of concentration of ASMq800(μg/mL)compared with 200(μg/mL)and 400(μg/mL),the A value of K562 /ADR cells gradually decreased,the growth curve suggested that with the increase of ASMq concentration in K562 /ADR cell,survival time was shortened,the inhibition of the cells was significantly increased.Different concentrations of ASMq on the adriamycin toxicity with increase of concen-tration of adriamycin,the inhibition of ASMq on multidrug resistance cell line was significantly higher.The reversal multiples calculated that with the increase of ASMq concentration 600 μg/mL and 900 μg/mL,the reversal index increased significantly 2.485 times and 3.651 times.Conclusion ASMq on human leukemia multidrug resistance cell line K562 /ADR could reverse the drug resistance,the reversal mechanism needs further study.