Aim To investigate the antagonistic action o f total saponins of panaxnotoginseng(PNS) on cardiac hypertrophy and its nervous mechanism.Methods (1)cardiac hypertrophy of rats due to pressure overload was induced by constricting of abdominal aorta. There were five groups in the experiments. The rats in Group A(control group)were sham operated . Group B was aorta-constricted group.The rats in Group C,D,E were given ip PNS 50,100,150 mg?kg?d -1 for three weeks respectively. Three weeks later, We measured the heart-weight(HW),left ventricular weight(LVW), the ratio of HW/BW,LVW/BW (LVI) and the cardiomyocyte diameters(MD) after dyeing by HE color.(2)The effects of PNS on the fast excitatory postsynaptic potential(f-EPSP),membrane depolarization induced by application of acetylcholine (ACh),membrane potential and membrane resistance of the isolated Stellate ganglion(SG)of the rats were investigated by means of intracellular recording techniques. Results (1)HW/BW, LVI and MD of Group E were significantly lower than that of Group B(P0.05).(2)At the concentration of 0.10 ～0.16 g?L -1, PNS reversibly depressed the amplitude of f-EPSP, but the ACh depolarization,membrane potential and membrane resistance were not significantly altered by PNS. Conclusion PNS can prevent cardiac hypertrophy due to pressure overload in rats and this action may underline its inhibitory action on presyn aptic effect of regulating calcium influx.

ObjectiveTo observe the clinic effect of Xueshuantong in subacute cerebral hemorrhage. Methods60 patients with cerebral hemorrhage were randomly divided into treatment group and control group, each grous 30 cases. The control group, only to conventional treatment, the control group addded Xueshuantong 300mg intravenous drip. On the basis treatment in 7 ～21 days of cerebral hemorrhage both groups were checked the NIHSS score and head CT. Before and after the teatment. ResultsAfter 21days,the hematoma volume in the treatment group was ( 3.3 ± 2. 6) ml, the control group was( 7.2 ± 3.7 ) ml, Treatment group was significantly better than the control group (t =4. 102,P ＜ 0.05 ). NIHSS score of treatment group was (4.7 ± 2.8 ) points, the control group was ( 8.6 ± 3.3 )points. Treatment group was significantly lower than the control group( t =5. 329,P ＜ 0.05). ConclusionXueshuantong could promote the absorption of hematoma and the nerve function recovery in subacute cerebral hemorrhage.

Objective To discuss the clinical value and nursing of the three-endoscope in the treatment of eholedoeholithiasis. Methods 45 eases of choledocholithiasis patients who were treated with LCDE (three-endoscope) were named as the research group.56 patients who received traditional open ab-dominal surgery were set as the control group. The average hospitalization time and satisfaction degree with nursing were compared, t test and χ2 test were adopted. Results The average hospitalization time was shorter and satisfaction degree with nursing was higher in the research group than those in the control group. Conclusions The treatment of choledochohthiasis with three-endoscope is safe and feasible, es-pecially when combined with antibiotics lavage and stone dissolution through naso-biliary duct.The opera-tion can widen the surgical indication,reduce the risk of surgery with little damage,clear stones completely, reduce postoperative complicatioas,make patients recover faster, shorten the hospital stay and achieve the same or better treatment results when compared to the traditional open abdominal surgery.

Objective To evaluate the curative effect and safety of Niaoshit on g pill in the treatment of urinary calculus.Method Multi- center randomized co ntrolled clinical trial was adopted. Three hundred and twenty cases were accepte d to the study, in which 200 cases were treated by Niaoshitong pill and 120 case s by Shilintong tablet as control. The effect of both groups was observed. Resul t 107 cases (53.5 % ) were cured, 53 cases(26.5 % ) effective, the total effe ctive rate being 80.0 % in the treatment group, and 27 cases(24.5 % ), 42 cas es (38.2 % ), and 62.7 % respectively in the control group. In a open group of 120 cases ,54 cases (45.0 % ) were cured, 44 cases (36.6 % ) were effective , the total effective rate being 81,6 % .Conclusion Niaoshitong pill can mark edly improve the clinical symptoms and exerts a strong lithagogue effect. It can promote the elimination of calculi after external blast lithotrity or ureterosc opic lithotrity, prevent the formation of 'stone street', and reduce the strictu re formed by the damage of ureter.

Child ; Child, Preschool ; Humans ; Vestibulocochlear Nerve Diseases ; diagnosis ; prevention & control

BACKGROUND: Panax notoginseng saponins (PNS) could significantly improve the learning and memory ability of rats, but its influence to peripheral nervous system still needs further investigation.OBJECTIVE: To observe the effects of PNS on the fast-excitatory postsynaptic potential (f-EPSP) in stellate ganglion (SG) of rats.DESIGN: Observation and controlled trial.SETTING: Pharmacological Laboratory of Guangxi Medical University.MATERIALS: The experiment was carried out at the Pharmacological Laboratory of the Experimental Center of Guangxi Medical University from January 2005 to February 2006. Thirty healthy male SD rats of clean grade and (220±20) g, provided by the Experimental Animal Center of Guangxi Medical University; SEN-7203 digital three track strip stimulator, microelectrode amplifier (MEZ8301, Japan NIHON KOHDEN COMPANY); glass microelectrode puller, and microelectrode manipulator, both the products of Narishige Company, Japan; PNS, provided by Kunming Jacobson Pharmaceutical Co., Ltd, and acetylchloride chline (Ach), the product of Sigma, U.S.A.METHODS: After the animals were executed acutely, their chest wall was opened to isolate SG rapidly under microscope, which was transferred to the perfusion chamber, and fixed with wire needles after peeling the connective tissue membrane. The ganglia were perfused continuously with the mixture of volume fraction 0.95 O2 and 0.05 CO2 plus Krebs solution with pH (7.4±0.05). Meanwhile, 0.08-0.16 g/L PNS was employed to perfuse and culture SG.①The glass microelectrode filled with 3 mmol/L KCI was used to puncture the isolated SG and record the amplitude and duration of depolarizing reaction of postsynaptic membrane.②PNS with the maximum concentration of 0.16 g/L, which could inhibit the f-EPSPs, was perfused to observe the effect of PNS on the amplitude and duration of depolarizing reaction of postsynaptic membrane induced by exogenous Ach (1 mmol/L, 1 minute).③PNS with the maximum concentration of 0.16 g/L, which could inhibit the f-EPSPs, was perfused to observe the effect of PNS on membrane resistance and membrane potential.MAIN OUTCOME MEASURES:①Amplitude of depolarizing reaction of postsynaptic membrane; ②Effect of PNS on the amplitude and duration of depolarizing reaction of postsynaptic membrane induced by exogenous Ach, and membrane resistance and membrane potential.RESULTS: Thirty rats were involved in the result analysis. ①PNS ranged 0.08 to 0.16 g/L could reversibly depress the f-EPSPs amplitude of, or change the forward active potential into f-EPSP; the higher the concentration of PNS, the more obvious the inhibition was. The depression appeared in 3-10 minutes after PNS perfusion, and the effect reached the peak at 0.16 g/L; f-EPSP was decreased evidently in 3 to 4 minutes. The inhibition nearly recovered to the control level after washing the ganglia with Krebs solution for 15 to 20 minutes. ②Effect of PNS on exogenous ACh-induced depolarization: The amplitude and duration of the Ach-induced depolarization did not significantly change before and 5 minutes after 0.16 g/L PNS perfusion [before: (15.5±2.4) mV, (256.1±21.5) seconds; after: (14.3±1.9) mV, (228.6±24.5) seconds, P＞0.05].③Effects of PNS on membrane potential and membrane resistance: The mean membrane potential and membrane resistance were not significantly changed after PNS perfusion [before:-(55.5±12.1) mV, (53.9±5.1) MΩ; after: -(54.3±10.4) mV, (55.1±4.8)MΩ, P＞0.05].CONCLUSION: PNS could reversibly depress the fast-excitatory postsynaptic potential in stellate ganglion of rats by presynaptic mechanism.

Objective To compare the curative effect between arthroscopic and open retrograde intramedullary interlocking nails in the treatment of femoral supracondylar fracture. Methods A total of 46 patients with femoral supracondylar fracture underwent the treatment by either arthroscopic (19 patients, Group A) or open (27 patients, Group B) retrograde intramedullary interlocking nails. Results The operation time, surgical blood loss and bone union time in the Group A were less than those in the Group B ( t =-4 193, -21 517 and -4 055, respectively; P =0 000). Follow-up for (10 3?3 6) months in the 46 patients revealed the results of therapy were, according to the Kolmert criteria, excellent in 15, good in 3, fair in 1 in the Group A while excellent in 7, good in 14, fair in 4 and poor in 2 in the Group B, without statistically significances between the two groups in the “good” or “excellent” rates (94 7% vs. 77 8%; ? 2 =1 345, P =0 246). Conclusions Use of arthroscopic retrograde intramedullary interlocking nails has advantages of shorter operating time, less blood loss and quicker bone union, benefiting the recovery of knee joint functions.

Objective To observe the efficacy of urinary kallidinogenase plus batroxobin in the treatment of acute cerebral infarction.Methods 105 patients with acute cerebral infarction were selected and divided into 3 groups:urinary kallidinogenase group (35 cases),combined treatment group (39 cases),batroxobin group (31 cases).The NIHSS score,Barthel Index,MRS score were evaluated before treatment and 10 days after treatment,and the clinical efficacy was compared.Results The NIHSS score significantly decreased after treatment in the three groups (all P ＜ 0.05).The effective rate of combined treatment group was better than that of the single treatment group (urinary kallidinogenase group or batroxobin group) [79.5 ％ (31/39),71.4 ％ (25/35),35.4％ (11/31) (x2 =15.801,P =0.001)].The Barthel Index and MRS score of combined treatmentgroup were better than those of the batroxobin group (P =0.003),not better than the urinary kallidinogenase group (P =0.766).Conclusion Urinary kallidinogenase plus batroxobin is effective in the treatment of acute cerebral infarction.

Abnormal activation of Notch1 plays pivotal roles in the molecular pathogenesis of human T-cell acutelymphoblastic leukemia (T-ALL). Activating Notch1 mutations present in over 60% of the T-ALL patients. However, so far,there is no therapy with little side effects that specifically targets the abnormally activated Notch1 pathway-induced T-ALL. Thepresent study briefly reviewed the effects of abnormal activation of Notch1 in the pathogenesis of T-ALL, as well as the currentapproaches targeting Notch1 and its limitations, thus providing some guidance for the research and development of clinicaltherapies targeting T-ALL.