Objective To identify potential candidate genes related to the cancerous phenotype by analyzing databases publicly available. Methods Using a data-mining tool called Digital Differential Display (DDD) from the Cancer Gene Anatomy Project database, ESTs from 17 different tumor types were analyzed for differential expression. Results We obtained 130 up-regulated and 159 down-regulated genes, most of which are related to cytoskeleton, ribosomal subunit, substance metabolism, cell cycle, signal conduction, transcription and translation. These genes appear most frequently on chromosome 12 but rarely on chromosome 21 and Y. Conclusion In silico identification is a high-throughput screening strategy. Our study may lay a foundation for identification of future caner markers and provide a new thought for screening strategy of cancer markers.

Objective Through analysis the amount and the types for the bacterial in the conjunctival sac between the warm meibomian gland massage and routine preoperative preparation for the inner eye operations,to compare the bacterial state before and after the nursing intervention,as to analysis of preoperative administration of the routine preoperative preparation on the effectiveness of prevention of postoperative intraocular inflammation,and so on peri operation period nursing intervention is effective in reducing or preventing endophthalmitis.Methods 40 patients(40 eyes) undergoing eye operation during March to December of 2013 in our hospital,they were divided into the experimental group and the control group,with preoperative warm meibomian gland massage the experimental group based in the implementation of internal eye operation on routine preparation.Based on all the patients in group (V1),on the morning of surgery (V2) before operation,after disinfection(V3),before the end of operation(V4),after the next morning(V5) and after 1 weeks of operation (V6) of the 5 conjunctival sac sampling cultured conjunctival sac fungi and bacteria population analysis.Results Two groups of cultured conjunctival sac and there were significant differences between the V1 and V6 for both groups in the amount and the tapes of the bacterial.Conclusions The inner eye operation patients with warm meibomian gland massage can effectively reduce or inhibit the bacterial amount after operation in the conjunctival sac,effectively reduce the occurrence of postoperative endophthalmitis of hidden dangers,this procedure should be schduled in the standerand SOP for the peri--operative preparation for the inner eye operations.

Objective In this report the proteomics technique was used for analysis of the Molecu-lar marker related acute rejection after liver transplantation.Methods Highly abundant proteins in human serum were deleted using Aurum Serum Protein Mini Kit and separated by 2-DE.The maps were analyzed by ImageMaster 2D Platinum software.The differential proteins on the gel were did in gel digestion with trypsin and then identified by nanoUPLC-ESI-MS/MS.Results Reproducible 2-DE image was successfully ob-tained.2 up-regulated expressed protein spots were identified clearly are serum amyloid A protein.Conclu-sions In this study, it was confirmed that SAA expression upregulate after transplantation in patients with acute rejection by proteomics technique, This method may provide clues to the early diagnosis of liver al-lograft rejection.

Objective: To analyze the cost of laparoscopy and laparotomy in the treatment of tubal pregnancy.Methods: The selected patients of tubal pregnancy were operated on in our hospital from Jan. 2000 to Jun. 2002. Eighty received laparoscopy and 13 received laparotomy.The off work day, hospital charge, cost of no work and total cost were analyzed and compared.Results: The mean hospital stay and off work days were significantly shorter in the laparoscopy group than in the laparotomy group( P

Objective: To introduce an improved direct method for first puncture and to study the safety of first puncture in laparoscopic surgery.Methods: Four kinds of techniques were used to perform the first puncture on 4 102 patients.Group A: trocar puncture were performed after pneumoperitoneum in 2 492 cases.Group B: trocar puncture was directly through the incision of umbilical skin in 798 patients; Group C: trocar puncture was directly through the incision of umbilic skin,subcutancous fat and fascia in 387 patients.Group D: opened umbilical hole and inserted the trocar shell without puncture in 425 cases.it was a success if entered the abdominal cavity at the first time,it was unsafe if punctured twice and a failure if the needle hadn't entered the abdominal cavity for 3 times or went into the subcutaneous vessels or viscera.Results: There were no vessels and viscera injury during punctures in our study.The puncture results were related to the different methods.Compared with group A, the success rate of group C and D were significantly improved( P 0.05).Success rates of group C and D were significantly higher than that of group A.The success rate was higher in group D than in group C( P

Sal-like protein 4 (SALL4), a newly discovered oncogenes, plays an important role in the formation of embryo, maintenance of embryonic stem cell pluripotency and self-renewal. SALL4 not only has a close relationship with hereditary disease, benign disease, malignant tumor, blood system tumor and germ cell tumors, but also chemoresistance and poor prognosis. SALL4 obtains biological functions by adjusting different signaling pathways, transcription factors and epigenetic modulation. Further mechanism study of SALL4 and its relationship with various diseases will provide new opportunities for the treatment of diseases.

Objective To investigate the influencing factors of stereopsis and ocular position changes before and after intermittent exotropia sur-gery. Methods A total of 70 cases diagnosed with intermittent exotropia(squint angle>15△and<85△)and treated in the Department of Ophthal-mology,the First Hospital of China Medical University from January 2010 to December 2014,were enrolled for the study. Operation methods was designed according to the pre-operative apparent distant strabismus ,and operations should be taken on the non-dominant eyes to lateral rectus re-cession combined with medial rectus shortening or lateral rectus recession. Patients were divided into different groups according to age ,age of exo-tropia onset,the angle of squint. The outcomes included best corrected visual acuity of eyes,refractive status,stereopsis,squint angle position of eye with intermittent exotropia after surgery. Results Compared with preoperative status,the stereopsis symptom was significantly improved after surgery(P<0.05). Stereopsis recovery and orthophoric rate were significantly increased according to the surgery age in children below 9 years old compared with those over 9 years old(P<0.05). Compared with the group of age≤9 years old,the rate of recovery of stereopsis and the rate of or-thophoria were not statistically significant(P>0.05). There was no statistical difference in the recovery rate of stereopsis and the orthostatic rate of eyes between the groups of 45△and more than 45△. Conclusion Intermittent exotropia surgery can effectively improve the stereopsis and eye po-sition,and the outcomes of surgery are related to the age of surgery. The recovery rate of stereopsis is higher in the patients with intermittent exotro-pia during the visual development period. Regardless of the size of the angle of squint ,early surgery is benefit to the recovery of stereopsis.

Acute liver failure and end-stage liver diseases are llfe-threatening.So far,orthotopic liver transplantation is the unique treatment available for these late-stage liver diseases,which,however,is limited by the lack of donor liver.While hepatocyte transplantation,bioartificial liver and tissue engineered liver are potential alternative treatments,the limited availability of functional hepatoeytes is the major hurdle.Due to their extensive capacity for self-renewal and pluripotency to differentiate into cells,embryonic stem cells represent a potential unlimited cell source for therapy.The clinical application of embryonic stem cells in liver diseases requires well-defined and efficient protocols for differentiation,characterization and purification in vitro.Hepatic differentiation of embryonic stem cells in different culttire systelns and the problems remained are reviewed in this article.

BACKGROUND:A cell mass from bone marrow can transform into oval cells and then differentiate into hepatocytes and biliary tract endothelial cells.It will bring a new hope for repairing irreversible damage of the liver.OBJECTIVE:To review several recent research results of the differentiation of bone marrow stem cells into hepatocytes.RETRIEVAL STRATEGY:A computer-based search was conducted in Pubmed and HighWire for English articles about bone marrow stem cells for liver ailments published between January 1999 and June 2007 with the key words of "bone marrow stem cells,mesenchymal stem cells,hemopoietic stem cells,hepatocytes,hepatic fibrosis,hepatic cirrhosis".Meanwhile,Vip Database was undertaken to identify the relevant articles on bone marrow stem cells in the treatment of hepatic cirrhosis and hepatic fibrosis published between January 1999 and June 2007 with the key words of "bone marrow stem cells,mesenchymal stem cells,hemopoietic stem cells,hepatocytes,hepatic fibrosis,hepatic cirrhosis" in Chinese.The data were selected primarily,and then quotations of each article were checked.Inclusive criterion:The articles related to curing hepatic fibrosis and hepatic cirrhosis with bone marrow stem cells were selected.Exclusive criteria:the articles with repetitive research or Meta analysis were excluded.A total of 49 related English articles and 42 Chinese articles were collected,34 literatures of them in accordance with the inclusive criterion were reviewed.Fifty-seven articles with old or repetitive articles were excluded.LITERATURE EVALUATION:Of the 34 inclusive articles,18 were on transplanting in vivo,12 were about cultivation and induction in vitro and 4 were about the problems and prospect.DATA SYNTHESIS:Bone marrow stem cells include mesenchymal stem cells and hemopeietic stem cells,which are characterized by plasticity.Growth factors in vitro and special microenvironment in vivo could induce the differentiation of bone marrow stem cells into the precursor of hepatocytes and mature hepatocytes.Moreover,hepatic function could be improved obviously.The superiorities of bone marrow stem cells included affluent source,low cost,little injury,without embolism and graft rejection in autologous transplantation,which offer a new style for curing hepatic diseases.Bone marrow stem cells would be the cell resource of artificial liver.CONCLUSION:Using bone marrow stem cells therapy for all kinds of refractory hepatopathy,such as hepatitis,liver cirrhosis and hepatoma,indicates a great achievement in clinic.

Acupuncture Points ; Acupuncture, Ear ; Child ; Child, Preschool ; Combined Modality Therapy ; Female ; Humans ; Male ; Moxibustion ; Urinary Incontinence, Urge ; physiopathology ; therapy ; Urination