OBJECTIVE: To develop a method for determination of the content of Dexamethasone acetate in Compound tretinoin ointment by RP-HPLC. METHODS: The separation was performed on C18 column with methanol: water (70∶30) as a mobile phase, wavelength of UV detector at 240nm. RESULTS: The linear range of Dexamethasone acetate was within 0.028 3～0.849 0?g(r=0.999 9). The average recovery of Dexamethasone acetate was 101.13%, with RSD at 1.28%. CONCLUSION: The method is simple, rapid and capable of producing good separation results. It can be applied to the quality control of Compound tretinoin ointment.

OBJECTIVE:To investigate situation and trend of proton pump inhibitors in 19 hospital of Hangzhou area and to provide reference for the research,production,sales and clinical application of this kind of drugs. METHODS:Utilization of proton pump inhibitors in 19 hospital of Hangzhou area during the period of 2004～2008 were analyzed statistically in respect of consumption sum,DDDs and DDC. RESULTS:Both consumption sum and DDDs of proton pump inhibitors increased quickly in recent five years. Amount of oral omeprazole(capsule and tablet)was the greatest among those categories and increased steadily. Amount of rabeprazole and esomeprazole as new type of proton pump inhibitors increased rapidly. CONCLUSION:Proton pump inhibitors have great potential for development. Omeprazole are widely used in the clinic as mature proton pump inhibitors.

Echocardiography, Three-Dimensional ; Heart Defects, Congenital ; diagnostic imaging ; therapy ; Humans

Objective To explore the clinical significance of gastric hyperplastic polyp pits under magnifying endoscopy. Methods Summary confirmed by magnifying endoscopy and pathological examination gastric hyperplastic po]yp's performance of magnifying endoscopy,Hp positive and the results of pathology during February 2004 to De-cember 2006. Results 55 patients found 116 gastric polyp,41.4% (48/116) with type A,26.7% (31/116) with type B,19. 8% (23/116) with type C,8. 6% (10/116) with type D,2. 6% (3/116) with type E,one case gastric pits blurred(type F) 0. 9% (1/116). 4 cases with type E and type F found moderate-severe atypical hyperplasia. Conclu-sion Gastric hyperplastic polyp pits with type E and type F ,their results of pathology to display atypical hyperplasia.

Objective To study the clinical effects of breast-preserving surgery in early-stage breast cancer. Methods From March 2002 to March 2007, 27 cases of early-stage breast cancer were treated with breast-preser-ving surgery. The control group had 28 cases, treated with radical mastectomy. Survival rate and recurrence rate were observed. Results The short-term progress of breast-preserving surgery in early-stage breast cancer had no significant difference with that of radical mastectomy. Plastic effects of breast were satisfied. Conclusion Breast- preserving surgery in early-stage breast cancer had favorable effects both in progress and in plastic. Therefore, it is warranted for wide use.

Objective To explore the influence of recombinant human erythropoietin(rhEPO) injection about nitric oxide(NO) and nitric oxide synthase(NOS) level in the brain and endothelin(ET) level in the blood after hypoxia-ischemia brain damage(HIBD) in neonatal rats,and elucidate the possible mechanism of the neuroprotective effect of rhEPO administered to the rat pup prior to cerebral hypoxia-ischemia.Methods Seven days-old SD rats were divided into control group,hypoxia-ischemia group(HI) and EPO group.NO activity,NOS level in the brain and ET in the blood were determined at 6 h after hypoxia-ischemia.Results NO and NOS levels in the brain and ET level in the blood were increased after HIBD in neonatal rats(P

OBJECTIVE:To compare the licensed pharmacist system in China and the USA,and to provide reference for im-proving pharmaceutical care and promoting public safety of drug use in China. METHODS:The relevant literatures in recent 10 years were retrieved from CJFD and Wanfang database. The differences of legal status,access qualification,legal obligations and responsibilities of licensed pharmacists in the USA and China were compared,and suggestions on improving the licensed pharma-cists system in China were put forward. RESULTS & CONCLUSIONS:At present,the problems of licensed pharmacist system in China are mainly that the legal status is not high,the access threshold is low,the legal obligations and responsibilities are not clear. However,the licensed pharmacist system in the USA has been developed for a century and formed a relatively strict legal sys-tem. Model State Pharmacy Act promulgated by National Association of Boards of Pharmacy in USA has clearly defined the access qualifications,legal obligations and responsibilities of licensed pharmacists. The pharmacy acts of the states were enacted on its blueprint. In view of current problems in China,it is necessary to learn from the above experience of the USA to improve the legal status,access qualification,legal obligations and responsibilities of licensed pharmacists and the quality of pharmaceutical care.

Objective To observe the connections between enkephalin immunoreactive(ENK-ir) terminals and ?-aminobutyric acid immunoreactive(GABA-ir) neurons in the rostral portion of the nucleus tractus solitarious(rNTS) of the rat. Methods Double-immunofluorescent labeling method and the pre-embedding immunohistochemical staining combined with immuno-gold particles labeling technique for electron microscopic detection were used in the present study. Results Under the laser scanning confocal microscope,dense ENK-ir fibers and terminals and some GABA-ir neurons were observed in the rNTS.Close contacts between ENK-ir terminals and GABA-ir cell bodies were also detected.By using the electron microscopic technique,ENK-ir reaction products were found to be mainly localized on the surface of round clear vesicles and dense-cored vesicles in the axon terminals.ENK-ir terminals formed symmetric,which was a dominant pattern,and asymmetric synaptic connections with GABA-ir and immunonegative cell bodies and dendrites.Conclusion The ENK-ir terminals might take part in the transmission and regulation of the taste information in the rNTS through inhibiting,exciting the GABAergic neurons or inhibiting directly the activity of neurons within the rNTS.

0.05)but a statistical difference between groups I and groups III(P