Objective:To investigate the endocrine functions of the islet ? cells and insulin sensitivity in the adult male rats,with intrauterine growth retardation induced by maternal starvation during pregnancy.Methods:The foods were available ad libitum throughout the pregnancy to the control group(n=8) and the rats in the experimental group(n=9) were fed 50% of the control group.The average birth weight of the offspring in the experimental group were below the mean values of the birth weights of the control group more than 2SD,defined as IUGR.The glucose tolerance test and hyperinsulinemic-euglycemic clamp were done in the adult male offspring(n=9 for each group).Results:(1)Litter size was not affected by maternal undernutrition.The birth weights of the rats in the maternal starvation during the pregnancy were significantly lower(4.37?0.34g)than those in the control group(6.42?0.29g) as defined as IUGR.The postnatal survival rate of the IUGR rats(75.4%) is significantly lower than that of the normal offspring(94.9%).The mean weight of the IUGR male rats was still lower than that of control group (279.8?30.3g vs 341.3?36.6g,P

Objective To observe the effects of storage condition on the quality of Semen Armeniacae Amarum. Methods Orthogonal design was adopted. The storage condition was optimized with temperature,humidity and storage time as the observation factors,with the content of amygdalin,and the content,acid value and peroxide value of fatty oil as the indexes.The content of fatty oil,acid value and peroxide value were measured according to the methods provided by 2005 edition of Chinese Pharmacopoeia. The content of amygdalin was measured by HPLC. Results Temperature was the main influencing factor of amygdalin content,then came humidity and storage time. Temperature was also the main influencing factor of the concent and the acid value of fatty oil,and the humidity was the second. For peroxide value,humidity was the main factor,then came temperature and storage time. Conclusion The optimal conditions of storing Semen Armeniacae Amarum as follows:storage in dry place with the temperature of 2～ 8 ℃,and in short storage time.

Objective: To investigate the islet ?-cell function and insulin sensitivity in adult male rats born intrauterine growth retardation caused by uterine placenta insufficiency. Methods: Bilateral uterine artery ligation (UAL) was performed on day 17 of gestation in the pregnant Wistar rats; sham-operated pregnant rats served as controls. The birth weights of the offspring in the UAL group were below the mean values of the birth weights of the control group more than 2SD, defined as IUGR. The tests were done in the adult male offspring (n=9 for each group). The glucose tolerance test was processed and Modified Beta-Cell function Index (MBCI) was calculated to evaluating the islet ?-cell endocrine function. Hyperinsulinemic- euglycemic clamp, the gold standard method to test the insulin resistance in the periphereal tissue was performed and insulin induced glucose infusion rate (GIR) was used to evaluate the insulin sensitivity. Results: (1) Though the mean birth weight of the newborn in the UAL group(4.49?0.56)g is much more than two standard deviations below the mean weight of that of the control group(6.16?0.30)g,P

Objective To study the clinical characteristic and the surgical treatment of the patients with familial adenomatous polyposis(FAP). Methods Retrospective analysis was made on the clinical feature and operative procedures of 28 patients with FAP in our hospital in recent 14 years. Of the 28 patients, 20 patients had familial history, 6 had cancerization. Results All the patients received operation, ileal pouch- anal anastomosis(IPAA) were performed on 25 cases(89.29%) , 3 cases underwent other operations. No severe complications had taken place postoperatively. All the patients received non-steroid anti-inflammatory drugs(NSAIDs) or tamoxifen treatment. All the patients were followed-up for 2-14 years, 4 patients died(14.29%) during follow-up period. Life quality and bowel function after operation were good in the aliving patients. No polyp in ileum pouch was seen. Conclusions Surgical treatment is the main methods for FAP, it can prevent bowel polyp carceration. Use of NSAIDs or tamoxifen as an adjuvant treatment of FAP is useful in the prevention of FAP recurrence.

With the empirical research methods,the author conducted detailed research into the school-running characteristics of Guangzhou University of Traditional Chinese Medicine.From the connotational perspective,the school-running characteristics can be summarized as follows: the progressive autonomy of the scientific orientation,developing the advantaged academic disciplines,advocating classic and the applied characteristics and the doctor-teacher characteristics.

The article introduced detailed research in the school-running characteristics of Guangzhou University of Traditional Chinese Medicine and from the extensional perspective summarize the school-running characteristics as follows:cultivating and training disease-cured Chinese clinical doctor,carrying out modernizational research of Chinese medicine; and establishing the southern-characteristic Chinese medicinal institute.

Critical Illness ; therapy ; Fluid Therapy ; Humans

Objective To observe the effect of enhanced extracorporeal counterpulsation (EECP) on intraocular pressure (IOP). Methods 25 patients were measured their IOP bilaterally with Schoitz tonometer before and after EECP. Results The IOP decreased in both left and right eyes after EECP (P<0.01). Conclusion EECP can reduce intraocular pressure.

Objective To construct vectors based on adeno-associated virus type 2 (rAAV2) carrying bone morphogenetic protein-7 (BMP-7) and observe their expression in adipose-derived adult stem cells, which can be served as a new gene therapy method and cell source for bone tissue engineering. Methods The coding sequence (1. 3 kb) of BMP-7 was amplified by PCR from the pcDNAl. 1 ( + ) plasmid containing the human BMP-7 cDNA. After purified, the gene fragments were cloned into a plasmid pUC18 and termed plasmid pUC18-hBMP7. The recombinant pUC18-hBMP7 was digested and further ligated to the pSNAV by T4DNA ligase and termed plasmid pSNAV-hBMP7. BHK-21 cells were transfected with the purified pSNAV-BMP7 plasmid according to a standard calcium phosphate precipitation method. The cells were isolated and the integrity of hBMP7 gene was determined by polymerase chain reaction (PCR) using the above PCR primers. To package the virus, stably transfected BHK-21 cells were subsequently infected with recombinant herpes simplex virus type 1 (rHSV-1). The collected cells were processed by chloroform treatment, PEG8000/NaCl precipitation and chloroform extraction for purification. The tiler was determined using quantitative DNA dot blots and the purity was examined by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. Following infection with rAAV2-BMP7 at multiplicities of infection of 1?105 vector genomes per cell and subsequent culture, adipose-derived adult stem cells were assessed qualitatively for BMP7 production. Results The recombinant plasmid pSNAV-hBMP7 was identified by PCR and digested with restriction enzyme. Transfection showed an efficiency of 90 % in ADAS cells. BMP-7 expression in ADAS cells was identified by Western blot. Conclusions The hBMP-7 recombinant adeno-associated virus vectors can be successfully constructed in vitro and rAAV2-hBMP7 can infect ADAS cells.