1.Research and practice of computer basic course at medical colleges
Jie AN ; Yan SHAN ; Shuang LI ; Hao CHEN
Chinese Journal of Medical Education Research 2011;10(5):579-581
Through the research and practice of computer basic course at medical colleges, the paper summarizes the experience of using modern technical means, to reform the traditional teaching ideas, teaching methods, teaching means and teaching management, to diversify teaching resources sharing. It also analyzes the shortcomings and makes recommendations to further strengthen the course development.
2.Effect of sequoyitol on expression of NOX4 and eNOS induced with glucose in human umbilical vein endothelial cells.
Xian-Wei LI ; Wei HAO ; Yan LIU ; Jie-Ren YANG
Chinese Journal of Applied Physiology 2014;30(2):147-152
OBJECTIVETo investigate the protective effect and mechanism of sequoyitol (Sep) on high glucose-induced human umbilical vein endothelial cells (HUVECs) injury.
METHODSHUVECs were cultured with high glucose (30 mmol/L) in the presence or absence of sequoyitol (0.1, 1 and 10 micromol/L) for 24 h. Cell proliferation was measured by BrdU marking and cell cycle was detected by flow cytometry. 2', 7'-dichlorofluorescein diacetate was used to evaluate intracellular reactive oxygen species (ROS) levels. The NO, malonydialdehyde (MDA) and H2O2 levels were determined by colorimetric method according to the manufacturer's instructions. The expression of endothelial nitric oxide synthase (eNOS) and NADPH oxidase 4 (NOX4) were measured by real-time PCR and Western blot.
RESULTSIn the present study, we found that sequoyitol pretreatment for 1 h significantly decreased cell injury, promoted cell proliferation. Meanwhile sequoyitol significantly down-regulated NOX4 expression and decreased the level of ROS, MDA and H2O2 and obviously increased NO levels and up-regulated eNOS expression.
CONCLUSIONSequoyitol alleviates high glucose-induced cell injuries in HUVECs via inhibiting oxidative stress and up-regulating eNOS expression.
Cell Proliferation ; Cells, Cultured ; Glucose ; toxicity ; Human Umbilical Vein Endothelial Cells ; drug effects ; metabolism ; Humans ; Hydrogen Peroxide ; metabolism ; Inositol ; analogs & derivatives ; pharmacology ; Malondialdehyde ; metabolism ; NADPH Oxidase 4 ; NADPH Oxidases ; metabolism ; Nitric Oxide Synthase Type III ; metabolism ; Oxidative Stress ; Reactive Oxygen Species ; metabolism
3.Effect of sequoyitol on expression of NOX4 and eNOS in aortas of type 2 diabetic rats.
Xian-Wei LI ; Wei HAO ; Yan LIU ; Jie-Ren YANG
Acta Pharmaceutica Sinica 2014;49(3):329-336
The aim of the present study is to investigate the effects of sequoyitol (Seq) on expression of eNOS and NOX4 in aortas of type 2 diabetic rats. Type 2 diabetic rats induced by high fat and high sugar diet and low dose of streptozotocin (STZ, 35 mg x kg(-1)) and were administered Seq (12.5, 25 and 50 mg x kg(-1) x d(-1)) for 6 weeks. The fasting blood glucose (FBG) and body weight were tested. Acetylcholine (Ach) induced endothelium-dependent relaxation and sodium nitroprusside (SNP) induced endothelium-independent relaxation were measured in aortas for estimating endothelial function. Aortic morphological change was observed with HE staining. The level of serum insulin was measured by radioimmunoassay. The total antioxidative capacity (T-AOC), malondialdehyde (MDA) and NO levels in aortas were determined according to the manufacturer's instructions. In addition, the expressions of eNOS and NOX4 in aortas were measured by immunohistochemisty, real-time PCR or Western blotting. The results showed that Seq significantly decreased FBG and insulin resistance, and improved aortic endothelium-dependent vasorelaxation function. The expressions of NOX4 and MDA content were obviously decreased, while the expression of eNOS, the levels of NO and T-AOC increased significantly in aortas of diabetic rats with Seq treatment. In conclusion, Seq protects against aortic endothelial dysfunction of type 2 diabetic rats through down-regulating expression of NOX4 and up-regulating eNOS expression.
Animals
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Aorta
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metabolism
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pathology
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Blood Glucose
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metabolism
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Body Weight
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Diabetes Mellitus, Experimental
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chemically induced
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metabolism
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physiopathology
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Diabetes Mellitus, Type 2
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chemically induced
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metabolism
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physiopathology
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Hypoglycemic Agents
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pharmacology
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Inositol
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analogs & derivatives
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pharmacology
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Insulin
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blood
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Insulin Resistance
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Male
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Malondialdehyde
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metabolism
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NADPH Oxidase 4
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NADPH Oxidases
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metabolism
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Nitric Oxide
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metabolism
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Nitric Oxide Synthase Type III
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metabolism
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Oxidation-Reduction
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drug effects
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Rats
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Rats, Sprague-Dawley
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Streptozocin
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Vasodilation
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drug effects
4.Analysis of clinical and pathological features of primary nephrotic syndrome in elderly patients
Yali ZHANG ; Jie FENG ; Yan LI ; Dapeng HAO ; Xueliang FENG
Chinese Journal of Geriatrics 2013;32(8):843-846
Objective To study the clinical and pathological features of primary nephrotic syndrome in elderly patients.Methods Clinical data of patients with primary nephrotic syndrome aged ≥ 60 years underwent renal biopsy were retrospectively analyzed and patients with primary nephrotic syndrome aged <60 years were selected as control group.Results Male patients with primary nephrotic syndrome were common in the elderly group and control group,and there was no significant difference in gender composition between the two groups (62.0% vs.61.5 %,P>0.05).The degree of edema and hypertension,levels of blood urea nitrogen,serum albumin,blood IgG and ratio of IgG /IgM were higher while levels of urinary protein and blood cholesterol were lower in elderly group than in control group (all P < 0.05).No significant differences in the incidence of hematuria,serum levels of creatinine,IgA,and complement were found between the two groups (all P>0.05).The risk of primary nephrotic syndrome was higher in elderly group than in control group (P<0.01).The level change of blood IgG was positively associated with plasma albumin,while negatively associated with urinary protein and blood cholesterol in both groups (r=0.327,-0.147,-2.860,respectively,all P<0.05).Membranous nephropathy was the most common type in elderly patients,accounting for 49.77%,while only accounting for 23.6% in control group,which had a significant difference between the 2 groups (x2 =62.390,P < 0.01).Mesangial proliferative glomerulonephritis was common in both groups,but no significant difference(x2 =62.390,P>0.05).Conclusions Male patients are more common than female patients in primary nephrotic syndrome.The clinical manifestations including urinary protein and serum albumin are milder but the risk is much greater in elderly patients than in the non-elderly patients.The change of blood IgG level is associated with urine albumin,plasma albumin and plasma cholesterol.Membranous nephropathy is the most common type followed by mesangial proliferative glomerulonephritis in elderly patients with nephrotic syndrome.
5.Thirteen cases of yawn symptom treated by Sun's transcranial repeat stimulation.
Yan-Jie SHANG ; Hao WU ; Yu-Ming WANG
Chinese Acupuncture & Moxibustion 2014;34(3):292-292
Acupuncture Therapy
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Adolescent
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Adult
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Female
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Humans
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Male
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Middle Aged
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Respiration Disorders
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physiopathology
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therapy
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Yawning
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Young Adult
6.A case of eumycetoma on the submaxilla due to Madurella
Jie YAN ; Jun DENG ; Fei HAO ; Bai-Yu ZHONG ; Qin-Jie LI ; Cun-Jian ZHOU
Chinese Journal of Dermatology 2003;0(08):-
Objective To investigate the clinicopathological features,diagnosis,treatment,prognosis and causative agent of a case of eumycetoma on the submaxilla.Methods A case of eumycetoma diagnosed in our department was assessed for its clinical and pathological features as well as mycologic and molecular identification.Related literature was reviewed.Results The patient was primarily characterized by swelling of the submaxilla,with multiple sinuses draining many black granules.Pathologic examination revealed a pyogenic granulomatous inflammation,and a number of lotus rhizome node-like hypha were observed in tissue samples through PAS staining.Sequence analysis of multiple loci of the isolates,including ITS 1,ITS2 and D1/D2,showed that it was mostly similar to Madurella mycetomatis with a homology of 97%.Conclusion This is a case ofeumycetoma on the submaxilla induced by a novel species of Madurella.
7.Teaching design of innovation experiment for closed-loop control of optogenetics
Hao CHEN ; Weiwei ZHANG ; An ZHOU ; Jie ZHANG ; Zhongxiang YAO ; Zhi'an HU ; Bo HU ; Jie YAN
Chinese Journal of Medical Education Research 2021;20(3):283-286
Combining with advances in optogenetics and feedback control of physiological function, we have utilized self-made PPDP (preview, presentation, demonstration, promotion) teaching method to clarify how various physiological functions are regulated by the nervous system and carried out physiological innovation experiment activities. The innovative experiments aim to cultivate students' self-study capability, broaden their vision, enhance their interest in physiology, and finally promote the effect of physiological theory teaching. We herein summarize our practice of closed-loop control of innovative experimental teaching in optogenetics from the following four facets: education concept, students and teacher resources, teaching design, and teaching experience. This summary is trying to explore new experiences of promoting students' participation in teaching activities and improving the teaching quality of physiology.
8.Support of acellular porcine corneal stroma for growth of corneal epithelium and stromal cell in vitro
Xu-Chu, LIN ; Yan-Nian, HUI ; Hao, MENG ; Yong-Jie, ZHANG ; Yan, JIN
International Eye Science 2008;8(7):1293-1295
AIM:To determine whether acellular porcine cornea stroma (APCS) could support the growth of the rabbit corneal cells in vitro.METHODS: APCS was prepared. The rabbit's corneal epithelium and stromal cells were cultured and seeded on, APCS in vitro.The observation of phase contrast photograph and histological examination were performed.RESULTS: Histological examination showed the epithe- lium grew on the scaffold of APCS in 2-3 layers at 10th day. The stromal cells adhered to the surface of the scaffold after 24 hours and invaded into the interlaminar of the material at 5th day.CONCLUSION: These results indicate that APCS can support the growth and proliferation of the corneal epithelium and stromal cells in vitro.
9.Effects of alkaloids from coptidis rhizoma on blood lipid metabolism and low-denstity lipoprotein receptor mRNA in golden hamsters.
Hao WU ; Yan-Zhi WANG ; De-Zhen WANG ; Jie PANG ; Xiao-Li YE ; Xue-Gang LI
China Journal of Chinese Materia Medica 2014;39(11):2102-2105
To study the effects of alkaloids from Coptidis Rhizoma on low-density lipoprotein receptor (LDLR) mRNA expression and antihyperlipedemic levels. The LDLR mRNA expression were detected by real time fluorescence quantitative PCR, and the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-c) and high-density lipoprotein cholesterol (HDL-c) in serum were measured at the first and last examination. The results show that, after the drug treatment, compared with the model group, each drug group showed a lipid-lowering effect. Especially, coptisine, palmatine, jatrorrhinze were significantly reduced TC, TG, LDL-c (P < 0.05, P < 0.01), and increased HDL-c (P < 0.01). In addition, they also increased mRNA expression of the LDLR in liver and HepG2 cells. The results showed that alkaloids from Coptidis Rhizoma can regulate lipid metabolism disorder, and coptisine have the best lipid-lowering effect.
Alkaloids
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administration & dosage
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Animals
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Cholesterol
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metabolism
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Cricetinae
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Drugs, Chinese Herbal
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administration & dosage
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Humans
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Hyperlipidemias
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drug therapy
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genetics
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metabolism
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Hypoglycemic Agents
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administration & dosage
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Lipid Metabolism
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drug effects
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Lipids
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blood
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Lipoproteins, LDL
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metabolism
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Mesocricetus
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Receptors, Lipoprotein
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genetics
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metabolism
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Triglycerides
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metabolism
10.Study on Foxp3 promoter region methylation of mononuclear cells in patients with rheumatoid arthritis
Yiqun HAO ; Xiumei LIU ; Xin YAN ; Jie YANG ; Zili FU ; Dongping LUO ; Kai WANG
Chinese Journal of Rheumatology 2013;17(4):264-267
Objective By detecting the expression levels of Foxp3 in CD4+CD25+ regulatory T cells of peripheral blood mononuclear cells (PBMCs) in rheumatoid arthritis (RA),and the Foxp3 gene promoter region methylation to explore its role in the pathogenesis of RA.Methods Twenty-five RA patients and 10 healthy controls were selected,and the PBMCs were extracted by density gradient centrifugation.Foxp3 expression levels of CD4+CD25+ regulatory T cells were detected by flow cytometry.The real-time fluorescence quantitative PCR assay was used to detect the Foxp3 mRNA expression in PBMCs; and bisulfate processing gene sequencing was used to determinethe differences in Foxp3 gene promoter sequence methylation level of PBMCs.The comparison between groups was analyzed using one-way ANOVA; two sets of qualitative data were compared using Fisher's exact test.Results The expression levels of Foxp3 mRNA in the CD4+CD25+regulatory T cells of active RA patients (2.31±0.25) was significantly lower than inactive RA group (3.68±0.26) and healthy controls (5.67±0.34),the difference was statistically significant (P<0.05).The Foxp3 mRNA expression level in inactive RA group was lower than that of the healthy controls (P<0.05).Foxp3 promoter region-67,-74 sites of methylation level in PBMCs of RA patients (46%) was significantly higher than that of the healthy controls (6%).Conclusion Reduction in the number of CD4+CD25+ regulatory T cells may be involved in the pathogenesis of RA and Foxp3 gene promoter methylation levels plays a key role in this process.