Objective To investigate the effects of USP4 on the proliferation of human hypertrophic scar fibroblasts(HSFB).Methods HSFB were cultured in vitro.The fourth generation of HSFB in logarithmic growth phase was selected in the experiment.HSFB were intervened by Vialinin A which was the inhibitor of USP4 for 0,12,24,48 h,then collected cells and the their expression of USP4 and TβRI and Smad7 protein was detected by Western blot.MTT assay was used to detect the effect of Vialinin A on the proliferation of HS fibroblasts and the cells were divided into experimental group and control group(without intervention).Results After treatment with Vialinin A in HSFB,the expression of USP4 and TβRI protein in HSFB decreased gradually,especially in 12 h(P<0.05),and Smad7 protein expression was increased gradually(P<0.05).The proliferative activity of intervened HSFB reduced gradually,the difference between experimental group and control group was statistically significant(P<0.05).Conclusion USP4 might inhibit the proliferation of scar cells and down-regulation of USP4 expression in hyperplastic scar fibroblasts,which can slow proliferative activity of intervened HSFB by regulating TGF-β/Smad signaling pathway.

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Through the research and practice of computer basic course at medical colleges, the paper summarizes the experience of using modern technical means, to reform the traditional teaching ideas, teaching methods, teaching means and teaching management, to diversify teaching resources sharing. It also analyzes the shortcomings and makes recommendations to further strengthen the course development.