Objective To investigate the impact of psoriacis on quality of life in patients with psoriasis. Methods A questionnaire interview included daily life and activities social activities and psychologic status were conducted of. Results 48 patients, 14 patients(29%) were impacted on quatity of life in heavy, 23 patiemts(48%) in middle, 11 patients(23%) in mildly no impact in zero. Conclusion The psoriasis valgaris influenced the Quality of life.

Objective The aim of this study is to investigate the effects of the initial amount of total RNA input,adapter dilution and PCR amplification cycles on the results of small RNA library preparation and high-throughput sequencing.Methods Based on the sequencing reads number,the number of detected miRNAs and the accuracy of quantitative detection,we explored the effects of initial Total RNA,dilution ratio and PCR cycles on the quality of microRNA library preparation and high-throughput sequencing,respectively.Results For libraries preparation of the normal RNA input(1 μg),the adapter dilution combined with 22 PCR cycles could gain the best quality of sequencing.In the low-input libraries(10 ng),adapter dilution and increased PCR cycles would also improve the quality of sequencing,but as compared with the 1 μg library,there was lower correlation with microarray quantitative results in general.Conclusions The initial amount of RNAs input has the biggest effects on the quality of sequencing,and the quantity accuracy rate of low-input libraries is lower than the normal libraries generally.Increasing the PCR amplification cycles properly is indispensable to low-input libraries.

Acupuncture Therapy ; Adult ; Female ; Humans ; Psoriasis ; therapy

Objective To observe the efficacy of the combination of moxibustion and pressing beans on auricular in the treatment of elderly patients with chronic diarrhea.Methods 45 elderly patients met the diagnostic criteria for diarrhea were divided into two groups,the treatment group (23 cases) received the treatment of combined moxibustion with ear pressure beans,the control group (22 cases) received traditional Chinese medicine treatment.The efficacy was observed and compared after two weeks.Results The effective rate reached 95.6％ in the treatment group,81.8％ in the control group (statistically significant difference,odds ratio 1.16).Conclusion Combined ear pressure beans with moxibustion treatment will receive a significant effect for the elderly patients with chronic diarrhea.The therapy is worth clinical application.

Objective To investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of glutamine synthetase (GS) of cultured rat retinal Müller cells in high glucose environment in vitro.Methods Mtüller cells were isolated from retinas of 10 Sprague-Dawley rats at postnatal day three to five by trypsin digestion,and were randomly divided into six groups,including normal control group,high glucose group,high glucose +5 U/ml rhEPO group,high glucose+ 10 U/ml rhEPO group,high glucose+ 20 U/ml rhEPO group,high glucose+40 U/ml rhEPO groups.After 48 hours,the apoptosis of retinal Müller cells were assayed by terminal transferase-mediated DNA end labelling assay,and the expression levels of GS protein were detected with semi-quantitative immunocytochemistry.Results Compared with the normal control group,the cell viability and GS protein were reduced while the cell death increased in Müller cells cultured in high glucose,the difference was statistically significant (t =27.4,P ＜ 0.01).Compared with the high glucose group,rhEPO treatment reduced the apoptotic Müller cells (t=857.2,2 374.6,2 473.2,2 537.7; P＜0.01),induced the expression of GS proteins (t=3.2,18.0,22.5,26.4; P＜0.05).Conclusions rhEPO can protect Müller cells from apoptosis under high glucose condition.The mechanism may be related to its function to up-regulate the GS protein expression,promote glutamic acid cycle,and reduce the excitotoxicity effects of high concentration of glutamate.

The fetal stage is a critical period for brain development. Intrauterine growth restriction(IUGR)af-fects the growth of fetus and impairs the brain development severely,resulting in long - term neurological sequelae such as motor and behavioral disorders. Since their brain underdevelopment originated in the uterus,postnatal interventions usually fail to improve the brain function. Then antenatal interventions become urgent to protect the brain,taurine has appeared a high practical value in improving the brain development. The adverse effects of IUGR on fetal brain develop-ment and the possibilities of antenatal interventions were introduced in this paper in order to improve the prognosis of the nervous system.

Objective To identify G?quadruplex structures in the promoter region of MET. Methods CD spectroscopy,UV spectroscopy,non?denatured electrophoresis and PCR stop assay were applied to indicate the G?quadruplex structure and its function. Results The Pu23WT se?quence in the promoter of MET adopted an intramolecular parallel G?quadruplex structure under physiological conditions in vitro,which can stop the extension of Pmet. Conclusion G?quadruplex structure in the promoter might inhibit MET gene expression in vivo.

Objective:To discuss the important role of clinical pharmacists in the prevention of medication errors and rational drug use through medication reconciliation in endocrinology department. Methods: The medication reconciliation was given to the diabetic patients over 70 years old newly admitted in the endocrinology department from January to April 2016, and the consistency and appro-priateness of medication orders were verified and the results were collected and analyzed by the pharmacists. Results: Totally 84 pa-tients were enrolled into the research with the average age of (76. 3 ± 5. 0) and the average number of combined diseases of (3. 5 ± 1. 2). The average medications taken by the patients before the admission were (6. 7 ± 2. 9), and 22. 6% of the patients took tradi-tional Chinese medicines and 4. 76% of the patients took health products. The patients whose medications for 24h needed intervention accounted for 27. 38%, the medications orders needed intervention accounted for 3. 67%, and the intervention success rate of clinical pharmacist was 100%. Conclusion:To the patients over 70 years old with type 2 diabetes and multiple diseases treated with a variety of medications, medication reconciliation in 24 hours can effectively prevent the medication errors and promote the rational drug use.

Objective To explore the clinical characteristics and treatment of methylmalonic acidemia(MMA) in order to improve our understanding of it.Methods We analyzed the clinical manifestations,laboratory examinations and treatments of 26 cases of methylmalonic acidemia in children.Results Twenty-four cases were involved in nervous system.Nine patients were involved in renal system.Eight cases of hematological involved.Liver enzyme elevated in 2 cases as well as the cardiac system were involved in 3 cases.One case was with pneumonia onset.The laboratory findings showed metabolic acidosis in 12 cases,hyperhomocysteinemia in 8 cases and remarkable elevation of urinary methylmalonic acid concentration in all cases.Some abnormalities in globus pallidus and cerebral white matter as well as diffuse cerebral atrophy were noted by the brain CT and MRI in 15 cases.Sixteen children have received therapy of vitamin B12,and supplementation of L-carnitine with restricted-protein diet.The follow-up for a period ranging from 3 months to 1.5 years( mean 8.5 months) of 15 cases with medical therapy showed a favorable outcome of nervous system improvement in 12 cases,however,2 patients died from severe metabolic acidosis.8 patients with renal involvement were normal in urine routine and renal function.Conclusion Methylmalonic acidemia has different clinical features,so early urine organic acids analysis by GC/MS method is essential Long-term and reasonable treatment after diagnosis is an effective way to improve the prognosis.