Objective:To discuss the role of Fas/FasL-mediated colonic epithelial apoptosis in the pathogenesis of ulcerative colitis(UC).Methods:Expression of Fas,FasL and M30(a new epithelium apoptosis marker) antigen on colonic mucosa of 35 patients with UC and 15 control populations were detected by IHC.Each marker was analyzed by image analysis system.Co-expression of Fas and M30 was observed through serial sections and double staining IHC.Results:The expression of Fas,FasL as well as M30 in UC increased considerably compared with controls (P

Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Pneumoconiosis ; epidemiology

ObjectiveTo explore the effect of Songlingxuemaikang combined with losartan on the blood pressure and quality of life in female patients with menopausal hypertension.Methods87 female patients with menopausal hypertension were randomly divided into combined group (Songlingxuemaikang combined with losartan group,44 cases) and the control group(losartan alone,43 cases).Before and after treatment the ambulatory blood pressure (ABMP) was monitored for 24h,and blood lipids and clinical symptoms were observed.ResultsAfter two months treatment,the blood pressure variability and nighttime blood pressure decrease rate of the combination group significantly reduced,and better than those of the control group( all P ＜0.05) ;The effective rate and efficiency rate of the combination group were 90.9％,63.6％ respectively,and was higher than 74.4％,39.5％ in the control group( x2 =4.15,5.06,all P ＜ 0.05 ).The clinical symptoms improving,total cholesterol ( TC ) reducing,density lipoprotein (LDL-C) lowing in the combination group was superior to the control group( all P ＜ 0.05).ConclusionSonglingxuemaikang combined with losartan in treatment of female patients with menopausal hypertension had a good antihypertensive effect,and could reduce blood pressure variability in menopausal women,improve the symptoms of menopause and lipid metabolism,incresse the quality of lfie of patients with menopausal hypertensive.

Objective To explore influence of antibiotics and Jiuyi Dan combined with incision-thread-drawing procedure on perianal abscess patients' level of activin A (ACTA) and C-reactive protein (CRP).Methods120 cases of perianal abscess patients in our hospital during March 2015 to December 2016 Coloproctology were selected, they were divided into the experiment group and the control group by random number method.All of patients were taken incision-thread-drawing procedure.Then the experimental group were given nine one antibiotics and Jiuyi Dan, the control group were given antibiotics.The cure rate, 6months after the recurrence rate, average healing time of the wound, and wound edema rate of two groups were observed.Levels of ACTA and CRP expression levels of two groups of patients before and after operation were analyzed.ResultsPatients in the experimental group cure rate was 96.7% higher than that of the control group (P<0.05), the recurrence rate of patients in the experimental group than in the control group, the difference was not statistically significant;the patients in the experimental group the average healing time was (20.67±3.22) d shorter than the average healing time of patients in the control group (29.48±3.15) d, patients in the experimental group wound edema rate of 5% was significantly lower than in the control group 20% (P<0.05);3D ACTA, the expression level of CRP was significantly higher than the preoperative 7 d levels after operation in the two groups (P<0.05);after 7d, the expression level of the two groups of patients with ACTA and CRP were significantly lower than the preoperative level of expression of 7d (P<0.05), experimental group levels of ACTA were significantly lower than the control group, the difference was statistically significant (P<0.05);postoperative 14d patients, ACTA levels of two groups were significantly lower than the levels before operation (P<0.05).ConclusionPerianal abscess can be treated effectively and patients levels of Acta and CRP were obviously dereased by Jiuyi Dan combined with incision-thread-drawing procedure combined, postoperative inflammatory reaction could be importantly alleviated, it was worth clinical promotion.

OBJECTIVE: To study the effect of samples having different pH values in the bacterial endotoxins test (BET). METHODS: The BET was conducted as an assay of the concentration of endotoxins by using kinetic-turbidimetric technique. Samples having different pH values but containing same concentration reference standard endotoxin (RSE) were tested by TAL/LAL reagents. The average result on the standard curve was calculated and the mean recovery observed. RESULTS: The reagents had three highly sensitivities in pH 5.20, pH 7.83/pH 6.23 and pH 10.55. After adjustment to pH 6 to 8, the mean recovery was 76.5%～115.9% or 85.8%～99.8%. When the sample pH value was less than 3 or more than 12, the test was unsuitable for the inhibition test, and the mean recovery was less than 56.8%. CONCLUSION: It is necessary to adjust the pH of the solution to be tested in the BET, adjust the sample to pH (6.5～7.5) when using TAL reagents, and adjust to pH (6.2±0.1) or pH(6.7～7.8) when using LAL reagents.

Objective To study the regulatory effect of paclitaxel injection on vascular endothelial related factors in serum of uterine cancer patients.Methods 30 cases of uterine cancer patients treated with paclitaxel (observation group)and 30 cases of uterine cancer patients treated with surgery (control group)were selected.Chemiluminescence immunoassay (CLIA)was used to detect expressions of serum tumor necrosis factor(TNF), Interleukin-8(IL-8)and tumor specific growth factor (TSGF).Results Before treatment,there was no significant difference between the serum TNF, IL-8 and TSGF in observation group and control group(P<0.05).At 3 months after treatment,the serum TNF,IL-8 and TSGFwere significantly lower than before treatment ,which was statistically significant(P<0.05).Conclusion Paclitaxel can decrease serum TNF,IL-8 and TSGF in patients with uterine cancer,which provides a new theoretical basis for its anticancer effects.

Objective:To observe oncosis in human peripheral blood T lymphocytes.Methods:Peripheral blood T lymphocytes of healthy adult was separated with Percoll(1.073 g/ml)and harvested by using nylon column.The cultured cells were divided into control and dexamethasone(DXM)group,and cell morphology was observed through light microscope,electron microscope and fluorescent microscope.And incidence rate of oncosis was analyzed by flow cytometry.Results:①Oncosis could be observed in cultured T lymphocyte after 96h.②In different concentrations of DXM group(1?10-6,1?10-5,1?10-4,1?10-3mol/L),The incidence of oncosis T lymphocytes was(3.49?0.42)%,(5.17?0.48)%,(8.44?0.72)%,(17.93?1.50)%.③During different cultured period(48,72,96,120h),The oncositic rate of T lymphocytes in DXM group was(0.53?0.10)%,(6.36?0.80)%,(20.60?1.59)%,(25.56?1.76)%.Conclusion:Oncosis can be seen in human peripheral blood T lymphocytes,and DXM could induce oncosis.

Objective To summarize the application and advancement of liver transplantation for hepatic metastasis from neuroendocrine tumor. Methods Domestic and overseas publications on the study of liver transplantation for hepatic metastasis from neuroendocrine tumor in recent years were collected and reviewed. Results Liver transplantation can offer good relief of symptoms, long disease-free intervals, and potential cure in individual patients with hepatic metastatic tumor. Important selection criteria are well-differentiated tumors and a low proliferation rate (Ki67

Periodontal ligament stem cells(PDLSCs) are not only the crucial type of cells in maintaining dynamic homeostasis and wound repair of periodontal tissues but also the basis for novel therapeutic strategy for periodontal regeneration.Although possessing the excellent proliferation and differentiation ability,PDLSCs behave differently under various conditions representative of several influential factors.Understanding of these influential factors will help us to exploit more about PDLSCs,and more importantly,lead a new modality in treating periodontal diseases based on stem cells.Here we review multiple important factors affecting function of PDLSCs and propose its use in future regenerative therapy of periodontal defects.