Objective:To investigate the effects of propofol on pyramidal neuron apoptosis in hippocampus of neonatal rats. Methods: Forty-twoseven-day-old male and female Sprague Dawley rats were randomly divided into three groups(n=14):group A received intraperitoneal 0.9% normal saline(7.5 ml/kg)once per day for seven days as control;group B received 0.9% normal saline(7.5 ml/kg)once per day for six days and propofol(75mg/kg)on the seventh day;group C received propofol(75 mg/kg)once per day for seven days. Two animals of each group were tested for blood glucose with tails cut off 3 hours after the last intraperitoneal injection,and six animals of each group were decapitated 5 hours after the last intraperitoneal injection for determination of caspase-3 expression with immuno-histochemistry. The other six animals of each group were decapitated after 3 weeks for microscopic examination. Results:Compared with group A,caspase-3 expression significantly increased while the number of pyramidal neurons decreased to different degree in both group B and C. More evident changes of the above were discovered in group C than that in group B,and the morphous of pyramidal neurons altered were only found in group C. Conclusion:The fact that increased caspase-3 expression leads to pyramidal neurons deletion to different degree was found in the rats which had received propofol.But the morphous ofpyramidal neurons altered onlywhen propofol had been repeatedlyinjected.

This paper describes the development and challenge of HIV/AIDS testing laboratory network and quality assurance system in China. At present, the HIV/AIDS testing laboratories includes three classes, the National AIDS Reference Laboratory, HIV/AIDS confirmatory laboratories and HIV/AIDS screening laboratories. All of them are accredited by the health authorities, and each class of laboratories take charge of their function strictly according to the "National Management of HIV/AIDS Detection (2006)". A complete quality assurance and quality control system for HIV/AIDS testing has been developed, which includes technical training, strict laboratory monitoring and approval,examination or proficiency testing on HIV/AIDS detection, and quality evaluation and supervision of HIV/AIDS diagnostic kits. Besides conduct the routine anti-HIV antibody test, more and more laboratories began to conduct other tests, such as CD4+ T lymphocyte cell counting, HIV viral load, HIV DNA PCR, genotyping, drug resistance, and HIV-1 recent infection test. The primary challenges faced by the HIV/AIDS testing laboratory network are in the areas of laboratory management and quality control. For example, the provincial PT program is inefficient, the internal quality control is conducted perfunctorily, personnel training can not met the needs of the workplace, which need to be improved.

Microvessel density (MVD) is the gold standard to evaluate tumor angiogenesis. Contrastenhanced sonography has been developing fast in recent years. By measuring perfusion parameters, it can represent the degree of new angiogenesis of gynecologic malignant tumors indirectly, and evaluate the biobehavior and prognosis of tumor. The advances of correlations between contrast-enhanced sonographic appearance and MVD of gynecologic malignant tumors were reviewed.

Objective: To establish an HPLC method for the determination of gastrodin, prim-O-glucosylcimifugin, 5-O-methylvisammioside, imperatorin and isoimperatorin in Yuzhen powder.Methods: A Phenomenex Gemini C18 column(250 mm×4.6 mm, 5μm) was used and the column temperature was 25℃.The flow rate was 1.0 ml·min-1 and the mobile phase consisted of methanol-water with gradient elution.The detection wavelength was 230 nm and the sample size was 5 μl.Results: The linear range of gastrodin was 2.68-214.0 μg·ml-1(r=0.999 9), and the average recovery was 100.2%(RSD=0.9%, n=6);the linear range of prim-O-glucosylcimifugin was 5.22-418.0 μg·ml-1(r=0.999 7), and the average recovery was 99.9%(RSD=0.9%, n=6);the linear range of 5-O-methylvisammioside was 4.57-365.8 μg·ml-1(r=0.999 5), and the average recovery was 99.7%(RSD=1.0%, n=6);the linear range of imperatorin was 5.22-417.2 μg·ml-1(r=0.999 6), and the average recovery was 99.0%(RSD=0.9%, n=6);the linear range of isoimperatorin was 5.29-423.2 μg·ml-1(r=0.999 8), and the average recovery was 100.2%(RSD=0.8%, n=6).Conclusion: The method is simple and accurate, and the contents of the five ingredients can be determined simultaneously.The method can be used for the quality control of Yuzhen powder.

A clinically isolated syndrome(CIS) is a term that describes a first clinical episode in which a patient has symptoms and signs suggestive of an inflammatory demyelinating disorder of the central nervous system.And it often affects optic nerves,the brainstem,or the spinal cord,and 30％-70％ of the CIS patients may develop multiple sclerosis(MS).Disease-modifying treatments may delay the development from CIS to MS.Their use in CIS is limited because of uncertain long-term clinical prognosis,treatment benefits and adverse effects.This article reviews the advances of predictive markers of conversion to MS after a CIS,in order to provide the reference for clinical treatment.

Objective To summarize the adverse events of Measles,mumps,rubella combined attenuated live vaccine (MMR) during 2008 in Changning District of Shanghai.To research the effective monitoring pattern of adverse event following immunization (AEFI),and to provide evidence for establishing the safe monitoring system.Methods Using MMR as the surveillance vaccine,the AEFI monitoring research with the active and passive methods was implemented.Results 95 reports were collected.Most of the adverse events were common.The imformation from the active method was more than the imformation from the passive method.It was established the communication mechanism of the Centers for Disease Control and Prevention (CDC) between the different levels.Conclusion Active and passive methods will improve the susceptibility and the utilization rate of data reporting.

Principles of constructing medical information technology curriculum system were pro-posed revolving round the core problem of how to combine needs of developing life science with culturing talents who can independently apply information technology knowledge in studying and researching. De-tailed implementing measures for constructing medical information technology curriculum system were summarized after repeated practical teaching. Longitudinal classification based on discipline and trans-verse classification based on teaching level were combined to construct the whole standard course. Finally, courses were appropriately distributed according to detailed culturing objectives for students in medical universities and colleges,thus a suitable medical information technology curriculum system in medical universities and colleges was formed.

Objective To study the influence of evidence-based health education in improving the ability of daily life and nursing satisfaction of patients.Methods A total of 80 schizophrenic patients were randomly divided into a control group (40 persons) and an experimental group (40 persons).The health education of routine ways was adopted on the control group.As for the experimental group,we posed questions,collected related literature and evidence through evidence-based support,set the health education based on the actual situation for individual and adjusted flexibly according to the affect of the health education during practice process.Two groups of patients were assessed by daily activity scale,positive and negative symptom scale weekly.We use questionnaire to compare with the satisfaction of two groups for patients when they left hospitals,including the disease-related knowledge,the satisfaction of the ways of the health education,the satisfaction of nurses.Using SPSS 18.0,the data between two groups were compared by t test,or Z test,or chi square test.Results After the implementation of evidence-based health education,the experimental group started at the second week,the PANSS scale score was lower than the control group,and the difference was statistically significant (P＜0.05).The score of experimental group'ADL scale compared with control group reached normal level in the third week and had statistic significance,P＜0.05.The questionnaire results showed that the average score of disease related knowledge and the average score of health education methods were higher in the experimental group than in the control group,and the difference was statistically significant (P＜0.05).In the evaluation of the most satisfactory nurses,patients' satisfaction in experimental group was higher than the control group,and the difference was statistically significant (x2=16.56,P=0.000).Conclusion The implementation of evidence-based health education for patients with schizophrenia can effectively improve their daily living ability,improve their mastery of disease-related knowledge and satisfaction with nursing.

Objective To research the binding specificity of new recombination toxin LHRH-PE40 and LHRH receptor on the surface of human colon carcinoma cell line Lovo and the mechanism of anti-proliferation,and determine the apoptosis.Methods Lovo cells were analysed by LHRH-PE40 marked with()~(125)Ⅰ;the cytocidal effect of the anti-tumor was evaluated by MTT assay,and the apoptotic rate was analysed by flow cytometry.Results Lovo cells had the binding of aglucone and receptor.Half lethal dose of human colon carcinoma cells Lovo with LHRH-PE40 was 0.24 mg?L~(-1).The apoptotic rate was increased when the LHRH-PE40 concentration was ranged from 0.1 to 10 mg?L~(-1)(P

Objective To analyze the adverse events following immunization(AEFI) in Changning District of Shanghai in 2006,evaluate the safety of vaccines,and provide the evidence for establishing the safe monitoring system of standard vaccination.Methods The AEFI cases were collected and reported by the Vaccine Adverse Events Surveillance System(VAESS).The data were classified as nonserious,serious reaction according to the symptoms and medical records.Results In 2006,178 AEFI cases related to 12 kinds of vaccines from 126459 doses of 19 kinds of vaccines.Among the adverse events reported,amount of nonserious and serious events were 166 cases and 12 ases respectively.The most commonyl reported type of non-serious adverse events were low-grade fever and non-serious local reaction.AEFI cases caused by DTP combined vaccine was in the first rank.Supplementary immunization of DTP combined vaccine had more cases of AEFI than routing immunization.Conclusions Establishing and perfecting the standardized VAESS will improve the susceptibility and the utilization rate of data on AEFI.To reduce the AEFI,we should implement vaccination regulations,distinguish contraindications,and introduce safer vaccines.