Objective:To investigate the preventive effect of nalmefene on sufentanil-induced cough in the patients with general anesthesia. Methods:Eighty patients with general anesthesia were randomly divided into the control group and the nalmefene group. The nalmefene group was intravenously given 0. 25μg·kg-1 hydrochloric acid natrium nalmefene 5 minutes before the induction, and physiological saline with the same capacity was given in the control group. Cough number and intensity in one minute after the injection of nalmefene were observed, and the changes of hemodynamic indices such as the blood pressure, heart rate and pulse oxygen satura-tion before the anesthesia induction (T0), 1min after sufentanil injection (T1) and after the intubation (T2) were observed and com-pared between the groups. Results:The incidence rate of cough was 37. 5% in the control group and 0% in the nalmefene group, and the incidence rate and strength of cough in nalmefene group were both lower than those in the control group (P<0. 05). The hemody-namic parameters at T1 showed notable changes when compared with those at T0 in the control group (P<0. 05), and had significant differences when compared with those in the nalmefene group (P<0. 05), and at T2, all the parameters recovered to the levels at T0. The parameters in the nalmefene group were much more steady than those in the control group (P>0. 05). Conclusion:Pretreatment with hydrochloric acid natrium nalmefene can prevent sufentanil-induced cough response during the induction of anesthesia without weakening the inhibitory effect of sufentanil on intubation response.

Humans ; Nerve Growth Factor ; physiology ; Pain ; physiopathology ; Pulpitis ; physiopathology ; Receptor, Nerve Growth Factor ; physiology ; Receptor, trkA ; physiology

Brain ; physiology ; Humans ; Magnetic Resonance Imaging ; Mastication ; physiology

Posterior capsular opacification (PCO) is one of the major complications of modern cataract surgery.Multiple studies had demonstrated that the extracellular matrix (ECM) plays an important role in the generation and development of PCO.A variety of ECM components,including fibronectin (FN),laminin (LN),collagen,lumican,osteoponin (OPN),decorin,hyaluronic acid and vimentin,affect the biological properties of lens epithelial cells (LECs) through different mechanisms,such as regulating cell migration,adhesion and proliferation.This review focuses on the role of ECM in PCO formation in order to explore the pathogenesis of PCO and provide a potential therapeutic approach for the prevention and management of PCO.

Objective To explore the correlation between chromosome fragile site in peripheral blood lymphocytes and proto-oncogene expression rate in patients with hepatocellular carcinoma.Methods The peripheral blood lymphocyte chromosome preparation was made routinely.The cells were cultivated with low concentrations of calf serum and folic acid,higher pH medium and G-banding method.The fragile sites in 20 patients with hepatocellular carcinoma(experimental group) and 18 healthy subjects(control group) were exactly located and analyzed.(Results The) fragile sites in experimental group were determined as 44 species mainly distributed on the chromosomes of A,B,C group.The expression rate of the fragite site in experimental group(9.90%) was significantly higher than that in control group(0.67%)(P

Aim To investigate the relationship be-tween phosphoprotein EBP50 and the proliferation of breast cancer in MCF-7 cells. Methods The quali-fied recombinant plasmid sh-EBP50-pGPU6/Neo was transfected into MCF-7 cells with EBP50 knocking down. The expression of EBP50, c-myc, p-ERK1/2, and ERK1/2 was detected by Western blot. The prolif-eration ability of cells was detected by sulforhodamine B assay. Results The EBP50 knocking down plasmid was constructed successfully. MCF-7 cells with EBP50 knocking down had been established successfully. Knocking down of EBP50 increased the proliferation of MCF-7 significantly, and partially augmented the ex-pression of c-myc and phosphorylation of ERK1/2 . However, knocking down of EBP50 did not impact the expression of ERK1/2 . Conclusion EBP50 suppres-ses the proliferation of breast cancer cell through inhib-iting the activity of ERK1/2 in MCF-7 cell line.

Objective To study the prognostic value of immunophenotypes in the patients with diffuse large B-cell lymphoma (DLBCL) and its prognostic significance of immunohistochemical markers for rituximab.Methods The data of 186 initial DLBCL patients were retrospectively analyzed.According to the international prognostic index,all patients were divided into relatively high-risk group and relatively low-risk groups,and the patients in both groups were divided into chemotherapy (CHOP) group and immune chemotherapy (R-CHOP) group,respectively,hnmunohistochemical method and Hans typing method were applied to immune classification in all of groups.The prognostic significances of the immunological subtypes in different prognosis group were compared by Log-rank test.Results The 5-year OS rate of the GCB subtype patients in the CHOP group was significantly higher than that of the non-GCB subtype (58.82 ％ vs 32.00 ％,X2 =8.482,P=0.004),while that was no significant difference between GCB and non-GCB subtypes patients in the R-CHOP group (72.97 ％ vs 61.54 ％,X2 =2.694,P =0.101).Further analysis results showed that non-GCB subtype patients treated with R-CHOP had significantly higher 5-year OS rate than those treated with CHOP (61.54 ％ vs 32.00 ％,X2 =7.385,P=0.007).In the 107 cases of low risk group,there was no significant difference in 5-year OS rate between GCB and non-GCB subtype patients in the CHOP group or in the R-CHOP group (all P ＞ 0.05),and there was no significant difference in 5-year OS rates between the CHOP and R-CHOP groups in the GCB group or in the non-GCB group (all P ＞ 0.05).In the 79 cases of high risk group,the 5-year OS rate in the GCB subtype group was significantly higher than that in the non-GCB subtype in the CHOP group (40.00 ％ vs 22.72 ％,X2 =3.978,P =0.045),while there was no significant difference in 5-year OS rates between GCB and non-GCB subtype patients in the R-CHOP group (62.50 ％ vs 42.31％,X2 =2.072,P =0.150).Conclusions By immunohistochemistry,DLBCL can be divided into two immunophenotype subgroups,which have guiding significance for early prognosis and therapeutic strategies,but which are no longer or weak in prognostic significance in the era of post-rituximab.R-CHOP improve significantly survival of non-GCB subgroup patients.However,its function needs to be further explored in the GCB subgroup.

Objective To investigate the organic pollutants in the contaminated water irrigation area soil and to study the lipid peroxidation effect of the pollutants on mouse. Methods The organic pollutants of the polluted irrigation soil and control soil were extracted using the supersonic oscillator. The ingredients of the organic pollutants were measured by the GC-MS spectrum. The 40 sanitary Kunming mice were randomly divided into five groups: the dimethylsulfoxide control group,low dose group of control soil,high dose group of control soil,low dose group of polluted soil,high dose group of polluted soil. The administered doses of the low and high dose groups were 25.5 g dry soil/kg body weight per day by oral for two weeks. The activities of T-SOD,GSH-Px and the content of MDA in serum of mice were determined. Results Phenanthrene,yrene,diphenyl,fluorene,naphthalene,dimethyl-naphthalene,2,3,6-trimethyl-naphthalene,anthracene,dimethyl-anthracene,1,4-dimethyl-anthracene,fluoranthene,4-methyl-pyrene,dibenzothiophen,4-methyl-dibenzofuran,dibenzofuran,butylated-hydroxytoluene,dodecane,neopentyl-cyclohexane,trimethyl-heptane,1,2-dimethyl-cyclohexanimine,butethal were detected in the polluted irrigation soil and their levels were 14.3,2.1,1.3,1.1,0.8,1.3,0.4,2.9,7.2,2.3,9.1,0.2,0.4,1.4,1.7,0.37,0.9,0.11,0.02,0.02 and 0.13 mg/kg respectively in the polluted irrigation soil. Trimethyl-heptane,dimethyl-heptane and 1,2-dimethyl-cyclohexanimine were detected,and their levels were all 0.01 mg/kg in control soil. Compared with the solvent of dimethylsulfoxide group,the activities of T-SOD and GSH-Px of the high dose group of control soil and two dose groups of polluted soil all decreased,the differences were all significant(P0.05). Conclusion The soil has been contaminated with organic pollutants in the investigated area and the soil extract may produce a certain effect on lipid peroxidation in mice.

Objectives To compare the effects of different early enteral nutrition formula on improving metabolic status of burned patients. Methods Thirty cases of burned patients with total burn surface area (TBSA) more than 30% were randomly divided into two groups:⑴Group F, enteral feeding with Fresubin 750MCT containing MCT/LCT as fat source, ⑵Group N, enteral feeding with Nutrison containing only LCT as fat source. All of the thirty patients received isocaloric, isonitrogenous and isolipidic enteral feeding with intragstric tube and enteral feeding pump, within 24h of postburn. Plasma insulin, glucagons, tumor necrosis factor (TNF) and 3-methylhistidine in urine were measured on day 1,4,7and 10 days of postburn. Results The results showed that the patients of group F had an increased blood concentration of insulin and the ratio of insulin/ glucagons, a decreased plasma concentration of TNF, and a reduced excretion of 3-methylhistidine in urine as compared with the patients of group N. Conclusions An enteral compound diet containing MCT/LCT in the early enteral feeding in burned patients can improve the metabolic status of burned patients more effectively.

Chemical Industry ; Humans ; Occupational Exposure ; Risk Assessment