Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Animals ; Cattle ; Humans ; Infant ; Infant Formula ; Milk Hypersensitivity ; Milk Proteins ; adverse effects

Objective To understand the difference of Demodex prevalence among medical students living and studying together with different classes, sexes and nationalities. \ Methods\ Demodex folliculorum and Demodex brevis were detected by using cellophane tape on the nasolabial grooves. \ Results\ The overall prevalence was 51\^5% in 2 248 medical students. Prevalence in different classes and sexes was: freshman 42\^6%, sophomore 49\^6% and third year students 66\^8%; 55\^7% in males and 48\^7% in females. Prevalence in students with different nationalities was: Mongolian 49\^8%, Han 52\^8% and other minorities 64\^3%. \ Conclusion\ The Demodex prevalence in students of senior classes was higher than those from junior ones (P

Objective To evaluate the effectiveness of music therapy on anxiety and adverse reactions induced by chemotherapy in breast cancer patients after modified radical mastectomy. Methods One hundred and ten breast cancer patients were divided into intervention group and control group. The patients in the intervention group received music therapy,progressive muscle relaxation and guided inner image in addition routine nursing care. State Anxiety Inventory and Rotterdam Symptom Checklist (RSCL) were administered before the 2nd chemo-cycle and before the 3rd chemo-cycle. Results There was trend of lower scores on the Rotterdam Symptom Checklist total score and the State Anxiety score in intervention group than in control group (P＜0.05). Conclusion Music relaxation therapy can alleviate chemotherapy related adverse reactions and anxious.Therefore,music relaxation therapy is a practical psychological behavioral intervention for the breast cancer patients during the chemotherapy after modified radical mastectomy.

The severity of coronary artery diseases is assessed based on morphological and functional stenosis.The study Oil detection of coronary artery diseases based on morphological lesions has unveiled its short comings for evaluation of the seriousness of the disease.Therefore,many research groups diagnose the coronary artery diseases by functional stenosis.From functional aspect,studies of x-ray angiography algorithms for blood flow and velocity measurement are potentially clinically important and are an actively researched subject,which Can accurately diagnose the coronary diseases from functional stenosis aspect.Based on the angiographic images,the article describes formulation and characteristics of existing coronary blood flow and velocity techniques such as tracking and computational methods.Tracking methods can be divided into four classes:bolus transport time algorithms,continuous velocity determination from two-dimensional(time-distance)parametric images,contrast traversal distance algorithms,and droplet tracking algorithms.Computational methods can be divided into six classes:indicator-dilution or Stewart-Hamilton method,first-pass distribution analysis,inverted continuity equation algorithms,inverse mass transport problem approach,optical flow type algorithms,and the fluid continuity method.

Objective To discuss changes of anxiety and adverse reaction before and after chemotherapy for patients with breast cancer, giving selective nursing intervention for coping with chemotherapy in order to reduce patients pain and increase life quality. Methods The changes of anxiety level and adverse reaction degree of 50 patients with breast cancer after modified radical mastectomy were investigated with questionnaires before the first, second and third time of chemotherapy. Results The postoperative patients showed significant difference in total score of Rotterdam symptom checklist, score of physiological domain, psychological domain and state anxiety, among which the adverse reactions such as lack of appetite, fatigue, lack of energy, nausea, acid ingestion, sore mouth, hair lost, irritability, depressed mood and tension increased after beginning chemotherapy. Conclusions Nurses should help patients to understand correctly chemotherapy and its possible adverse reaction, teach them correct coping method in order to reduce uncomfortable symptom, promote whole rehabilitation and increase life quality.

Biliary tract infection is the most commonly seen severe complication of malignant obstructive jaundice (MOJ)with a high mortality rate. Bile bacterial infection in MOJ patients is related with a variety of clinical factors and the therapeutic approaches including surgical operation,ERCP and percutaneous transhepatic cholangial drainage( PTCD). Bile bacterial culture combined with drug sensitivity test is of great importance for selection of optimal antibacterial agents. In this article,the related factors,current status of therapeutics,biliary bacterial spectrum and drug sensitivity in MOJ with biliary tract infection were reviewed for defining the clinical indicators and guiding the use of antibacterial agents.

Objective To build occupational management skills training mode for the community patients with psychosis in recovery stage by using action research method. Methods Action research method was applied to mental rehabilitation patients in Sunshine Psychiatric Garden of Shanghai to manage vocational rehabilitation support. By literature verification and empirical experience, the key problems were found out, and goals were established. Then management skills according to planning, action, observation, reflection and correction were implemented. This circle were spiraled for 2-3 times stages, and verification and evaluation before and after action practice based on Negative Symptom Rating scale (SANS) and Social Disability Screening Scale (SDSS). Results The community mental vocational rehabilitation was built with action research and based on the evidence of foreign psychic rehabilitation supportive individual placement and support. The mode should consider the professional characteristics of technical, social and economic. Also, it should contain professional ability, social skills and job adaptability training and performance evaluation, and offer salary allowance. The factors of SANS in 21 patients after practice were significantly improved (P < 0.01), and most of the factors in the SDSS were significantly improved (P < 0.01). Conclusions Community psychic vocational rehabilitation using action research method is a kind of advanced nursing practice, and also a process of evidence-based practice, which can provide a systematic, specific, practical work method for community mental rehabilitation institutions to force vocational rehabilitation training.

Objective To explore the relationship between calcium channel ?1 subunit (Cav1.1) gene intron 26 -67 A/G polymorphism and thyrotoxic periodic paralysis(TPP). Methods Cav1.1 gene polymorphism at position -67 was determined by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) in 46 male patients with TPP, 68 male Graves’ disease (GD) patients without TPP and 72 healthy male controls. The difference of genotype and the variation of allele frequencies were analyzed by Chi-square test. Results (1) Frequencies distribution of AG+GG genotype in TPP, GD and control groups were 47.83%, 14.71% and 29.17% respectively, and those of allele G were 44.57%, 13.24% and 27.78% for the three groups respectively. (2) Frequencies of -67 AG+GG genotype in TPP group were significantly higher than those in GD and CON group(OR=5.32, P

The effects of monoterpene perilly alcohol (POH) alone or in combination with STI571 on the proliferation and apoptosis of the cell line K562 positive for Bcr/Abl were investigated. By using cell culture, the effect of the drugs on the proliferation of the cells was studied. TUNEL and flow cytometry assay of FITC-Annexin V and PI labeled cells were applied to detect the effects of the drugs on the apoptosis of the cells. The results showed that at 36 h, IC50 of POH on K562 positive for Bcr/Abl and HL-60 negative for Bcr/Abl were 81.0 +/- 11.3 micromol/L and 113.6 +/- 23.4 micromol/L respectively (P>0.05). POH could inhibit the proliferation of K562 in a time- and dose-dependent manner with the inhibitory rate of 100 micromol/L POH on K562 cells at 36 h being (53.2 +/- 3.65)%. K562 cells were more sensitive to STI571 than POH. IC50 of STI571 on K562 cells in 36 h was (0.256 +/- 0.054) micromol/L. In a time- and dose-dependent manner, POH induced the apoptosis of K562 cells with the percentage of apoptotic cells by 100 micromol/L POH at 40 h being (21.0 +/- 3.3)%. Both 100 micromo/L POH and 0.2 micromol/L STI571 had the same inhibitory effects on the K562 cells at 36 h. But at 12 and 24 h, the inhibitory rate of POH was significantly higher than that of STI571 (P<0.05) and the ability of STI571 inducing apoptosis at 36 h was greater than that of POH. 50 micromol/L, 100 micromol/L and 200 micromol/L POH in combination with 0.2 micromol/L STI571 could obviously increase the inhibitory effects on the cellular proliferation. Combined use of 50 micromol/L, 100 micromol/L, 200 micromol/L with 0.2 micromol/L STI571 could strongly induced apoptosis, especially 200 micromol/L POH in combination with 0.2 micromol/L STI571. It was concluded that the antileukemia effect of POH had no obvious Bcr/Abl positive selectivity. POH can inhibit the proliferation of K562 and induce the apoptosis in a time- and dose-dependent manner. K562 cells were more sensitive to STI571 than POH. POH in combination with STI571 could obviously enhance the abilities of STI571 inhibiting the proliferation and inducing apoptosis of K562 cells.
Antineoplastic Agents/*pharmacology ; Apoptosis/*drug effects ; Dose-Response Relationship, Drug ; Drug Synergism ; Fusion Proteins, bcr-abl/analysis ; HL-60 Cells ; K562 Cells ; Monoterpenes/*pharmacology ; Piperazines/pharmacology ; Pyrimidines/*pharmacology