Objective To seek the best method for security precaution of elderly inpatients.Methods Elderly patients(296 cases) from January to August in 2006 and elderly patients(336 cases) from January to August in 2007 were divided into the control group and the improved group.The improved group received selective safety precaution measures after assessment of crisis degree by nurse security scale.The control group was given routine security nursing measures.The hospitalization time and incidence of unsafe events were compared in the two groups. Results The two groups were different in the following aspects:hospitalization time, incidence of unsafe events,satisfaction degree of family members,shift time of nurses,satisfaction degree of nurses. Conclusions To supply selective security nursing for elderly patients after assessment of crisis degree by nurse security scale at the admission day could decrease the incidence of un-safe events and hospitalization time.At the same time it shortened the shift time and increased shift efficien-cy to realize the win-win relations between nurses and patients.

ObjectiveTo discuss the feasibility and effect of clinical application of the single-bag and double-chamber anus tube.MethodsPatients who needed retention enema and non-retention enema(80 cases) were divided into 2 groups with 40 cases in each group.The test group was given enema by single-bag and double-chamber anus tube.The control group was given enema by traditional rubber anus tube.The reaction and clinical effect were compared between the two groups.ResultsIn the test group,pain and malaise lessened,intestinal cleaning was good,enema liquid reservation prolonged,the satisfaction degree of patients improved,compared with that of the control group (P<0.01).ConclusionsClinical application of self-made single-bag and double-chamber anus tube can give lesser pain and better effect of patients,and it improved satisfaction degree of patients and efficiency of nursing work greatly.

The international Non-governmental Organizations ( NGOs) have been playing an important role in the field of global health, which also represents a channel for countries' international experience. This research fo-cuses on the role of NGOs in global health, their governance tools, global health program management experience, and current status of development for Chinese NGOs. In light of international experiences, we suggest the Chinese government to support a few top NGOs to engage intensely in global health, build management and cooperation mechanism with NGO which will be considered as important complemertary role. The Chinese government shonld implement “major support and comprehensive improvement” strategy.

Objective To discuss the application effect of new physical therapy technique in the rehabilitation of patients with hand injury afte operation. Methods Patients with hand injury after operation (150 cases) were divided into the observation group (87 cases) and the control group (63 cases) randomly. The control group received routine treatment and nursing. The observation group was given irradiation by He-Ne laser therapeutic instrument, vinegar therapy and static electromagnetic field therapy. The rehabilitative effect after operation was compared between the two groups. Results The time for disappearance of pain and swelling in the obsevation group was shorter than that of the control group (P<0.05). Conclusions Application of new physical therapy technique in patients with hand injury afte operation could shorten time of rehabilitation and improve rehabilitative quality.

Animals ; Hedgehog Proteins ; metabolism ; Humans ; Liver Diseases ; metabolism ; pathology ; Signal Transduction

Congresses as Topic ; Otorhinolaryngologic Surgical Procedures ; Rhinitis ; surgery ; Skull Base ; surgery

Child ; Cystitis ; Eosinophils ; Humans ; Male

Humans ; Liver Cirrhosis

Objective　To study the effects of transforming growth factor-β1 (TGF-β1) and transforming growth factor-α (TGF-α) on the growth and invasive potentials in human bladder tumor cells.　Methods　The effects of TGF-β1 and TGF-α on the growth of EJ cell line and the effects on MMPs and TIMP-2 were studied by means of MTT,Western Blot and RT-PCR methods.　Results　(1)TGF-β1 and TGF-α tended to inhibit the growth of EJ cells but statistically nonsigficant.(2)Higher levles of MMP-9 mRNA but lower levels of MMP-2,TIMP-2,MT1-MMP mRNA were found in EJ cells following the treatment with TGF-β1.The same was true for the expression of MMP-2,TIMP-2 mRNA in TGF-α groups.However,MMP-9 mRNA was not found in both TGF-α groups and the control groups. (3)TGF-β1 (0.1,1.0 ng/ml) enhanced MMP-2 protein but not the TIMP-2 protein,while TGF-β1 (5.0,10.0 ng/ml)decreased TIMP-2 protein but not on MMP-2.In TGF-α groups,when the concentration was 1.0,5.0,10.0ng/ml,TIMP-2 protein expression was decreased but MMP-2 did not.When the concentration reached 100.0 ng/ml,it increased MMP-2 protein level,not the TIMP-2 protein.　Conclusions　TGF-β1 and TGFα do not inhibit the proliferation of EJ cells whereas the enhanced-invasiveness and metastasis may be associated with regulating the expression of MMPs and TIMP-2.

BACKGROUND: In the tooth regeneration and dental tissue engineering study, finding suitable stem cells are the central issues.OBJECTIVE: To isolate and culture child exfoliated deciduous tooth pulp stromal cells, and compare difference in dentin sialophosphoproteln expression prior to and following mineralization.DESIGN, TIME AND SETTING: The cell observational experiment was performed at the Central Laboratory of Daqing Oil Field General Hospital from December 2006 to December 2007.PARTICIPANTS: Dental pulp were obtained from exfoliated deciduous molar tooth of eight children aged from eight to ten, four males, four females. The possibility of infectious diseases, endocrine disease, dental caries and periodontal disease was excluded.METHODS: Deciduous teeth pulp stromal cells were obtained by tissue block adhesion method, and then cultured in DMEM with 10% fetal bovine serum for 14 days. Following digestion and passage, cells were incubated in DMEM/F12 supplemented with 50 pmol/L laevo-ascorbic acid, 10 nmol/L vitamin D3. 10 mmol/L Na β-glycerophosphate and 10-8 mol/L dexamethasone for another 14 days. Cells cultured in complete DMEM served as controls.MAIN OUTCOME MEASURES: Cell morphology and growth rule were observed under a microscope. Difference in dentin sialophesphoproteln expression was determined prior to and following mineralization using immunocytochemical staining.RESULTS: Dental pulp stromal cells from human exfoliated deciduous tooth, adhered to the wall, were polygon-shaped and characterized by large cell size and a relatively large nucleus and plenty cytoplasm after mineralization for 14 days. Over 80% cells were positive for dentin sialophosphoprotein. Non-induced cells were spindle, and only 2% cells were positive for dentin sialophosphoprotein.CONCLUSION: Odontogenic inductive culture might improve odontoblastic differentiation. It seems that human exfoliatod deciduous tooth pulp represents an new reservoir of adult stem cells with odontogenic potential.